• Journal of Microbiology and Biotechnology
• /
• v.22 no.12
• /
• pp.1644-1652
• /
• 2012

Iron plays a key role in host-pathogen interactions. Microbial pathogens require iron for survival and virulence, whereas mammalian hosts sequester and withhold iron as a means of nutritional immunity. We previously identified two paralogous genes, CFT1 and CFT2, which encode homologs of a fungal iron permease, Cft1 and Cft2, respectively, in the human fungal pathogen Cryptococcus neoformans. Cft1 was shown to play a role in the high-affinity reductive iron uptake system, and was required for transferrin utilization and full virulence in mammalian hosts. However, no role of Cft2 has been suggested yet. Here, we identified the third gene, CFT3, that produces an additional fungal iron permease homolog in C. neoformans, and we also generated the cft3 mutant for functional characterization. We aimed to reveal distinct functions of Cft1, Cft2 and Cft3 by analyzing phenotypes of the mutants lacking CFT1, CFT2 and CFT3, respectively. The endogenous promoter of CFT1, CFT2 and CFT3 was replaced with the inducible GAL7 promoter in the wild-type strain or in the cft1 mutant for gain-of-function analysis. Using these strains, we were able to find that CFT2 is required for growth in low-iron conditions in the absence of CFT1 and that overexpression of CFT2 compensates for deficiency of the cft1 mutant in iron uptake and various cellular stress conditions. However, unlike CFT2, no clear phenotypic characteristic of the cft3 mutant and the strain overexpressing CFT3 was observed. Overall, our data suggested a redundant role of Cft2 in the high-affinity iron uptake and stress responses in C. neoformans.

• The KIPS Transactions:PartB
• /
• v.12B no.2 s.98
• /
• pp.131-136
• /
• 2005

Motion Estimation (ME) has been developed to remove redundant data contained in a sequence of image. And ME is an important part of video encoding systems, since it can significantly affect the qualify of an encoded sequences. Generally, ME consists of two stages, the integer pixel motion estimation and the half pixel motion estimation. Many methods have been developed to reduce the computational complexity at the integer pixel motion estimation. However, the studies are needed at the half pixel motion estimation to reduce the complexity. In this paper, a method based on the correlations between integer pixel motion vectors and half pixel motion vectors is proposed for the half pixel motion estimation. The proposed method has less computational complexity than the full half pixel search method (FHSM) that needs the bilinear interpolation of half pixels and examines nine half pixel points to the find the half pixel motion vector. Experimental results show that the speedup improvement of the proposed method over FHSM can be up to $2.5\~80$ times faster and the image quality degradation is about to $0.07\~0.69(dB)$.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.8 no.2
• /
• pp.245-249
• /
• 2004

In this paper, we have proposed a clock recovery algorithm of Orthogonal Frequency Division Multiplexing/Quadrature Phase Shift Keying Modulation-Digital Microwave Radio(OFDM/QPSK-DMR) system using Band Limited-Pulse Shaping Filter(BL-PSF) and compared the clock phase error variance of OFDM/QPSK-DMR system with that of single carrier DMR system. The OFDM/QPSK-DMR system using windowing method requires training sequence or Cyclic Prefix (CP) to synchronize the clock phase of received signal. But transmit efficient is increased in our proposed DMR system because of no using redundant data such as training sequence or CP. The proposed clock recovery algorithm is simply realized in the OFDM/QPSK-DMR system using BL-PSF. The simulation results confirm that the proposed clock recovery algorithm has the same clock phase error variance performance in a single carrier DR system under Additive White Gaussian Noise(AWGN) environment.

• The KIPS Transactions:PartA
• /
• v.9A no.4
• /
• pp.451-458
• /
• 2002

In this article, we present, design, and implement a software and hardware for an embedded RAID system. The merits and drawbacks of our system are presented by performance evaluation. The proposed hardware system consists of three fibre channel controllers for the interface with fibre channel disks and hosts. Embedded Linux in which a RAID software is implemented is ported to the hardware. A SCSI target mode device driver and a target mode SCSI module are designed for that our RAID system is considered as a block device to a host computer. Linux Multi-device is used as RAID functions of this system. A data cache module is implemented for high performance and the interconnection between Linux Multi-device and the target mode SCSI module. The RAID 5 module of Multi-device is modified for improvement of read performance. The benchmark shows that the new RAID 5 module is superior to the original one in overall performance.

• Journal of KIISE:Computer Systems and Theory
• /
• v.35 no.2
• /
• pp.66-74
• /
• 2008

Since modem embedded systems need to access, manipulate or store sensitive information, it requires being equipped with cryptographic file systems. However, cryptographic file systems result in poor performance so that they have not been widely adapted to embedded systems. Most cryptographic file systems degrade the performance unnecessarily because of system architecture. This paper proposes ISEA (Indexed and Separated Encryption Approach) that supports for encryption/decryption in system architecture and removes redundant performance loss. ISEA carries out encryption and decryption at different layers according to page cache layer. Encryption is carried out at lower layer than page cache layer while decryption at upper layer. ISEA stores the decrypted data in page cache so that it can be reused in followed I/O request without decryption. ISEA provides page-indexing which divides page cache into cipher blocks and manages it by a block. It decrypts pages partially so that it can eliminate unnecessary decryption. In synthesized experiment of read/write with various cache hit rates, it gives results suggesting that ISEA has improved the performance of encryption file system efficiently.

• Journal of Korean Clinical Nursing Research
• /
• v.25 no.2
• /
• pp.170-178
• /
• 2019

Purpose: The purpose of this study was to determine the mediating effects of self-efficacy and the belief about medication on the association between depression and medication adherence in patients with systemic lupus erythematosus. Methods: 128 patients aged ${\geq}19years$, who were regular outpatients or admitted patients diagnosed with systemic lupus erythematosus at a tertiary hospital in B city, participated in this study. Data were collected by using a self-administered questionnaire. Testing of mediating effects was analyzed by a parallel redundant mediated model using the PROCESS macro for SPSS version 3.3. Results: They scored an average of $16.71{\pm}11.13$ for depression, $694.14{\pm}170.68$ for self-efficacy, $3.05{\pm}4.60$ for the belief about medication, and $90.14{\pm}15.37$ for medication adherence. The direct effect of depression on medication adherence was not statistically significant, but the indirect effects of depression mediated with self-efficacy and belief about medication were statistically significant. Conclusion: It is necessary to develop and apply a nursing intervention program that can not only relieve depression but also promote self-efficacy and the belief about medication with the objective of improving medication adherence among patients with systemic lupus erythematosus.

• The Journal of the Korea Contents Association
• /
• v.18 no.12
• /
• pp.35-45
• /
• 2018

Motion Estimation(ME) has been developed to reduce the redundant data in digital video signal. ME is an important part of video encoding system, However, it requires huge computational complexity of the encoder part, and fast motion search methods have been proposed to reduce huge complexity. Multi- view video is obtained by capturing on a three-dimensional scene with many cameras at different positions and its complexity increases in proportion to the number of cameras. In this paper, we proposed an efficient motion method which chooses a search pattern adaptively by using the temporal-spatial correlation of the block and the characteristics of the block. Experiment results show that the computational complexity reduction of the proposed method over TZ search method and FS method can be up to 70~75% and 99% respectively while keeping similar image quality and bit rates.

• KIPS Transactions on Software and Data Engineering
• /
• v.8 no.1
• /
• pp.27-36
• /
• 2019

In this paper, we propose a method to find the most similar answer to the user's response from the question-answer database in order to avoid generating a redundant question in retrieval-based automatic question generation system. As a question of the most similar answer to user's response may already be known to the user, the question should be removed from a set of question candidates. A similarity detector calculates a similarity between two answers by utilizing the same words, paraphrases, and sentential meanings. Paraphrases can be acquired by building a phrase table used in a statistical machine translation. A sentential meaning's similarity of two answers is calculated by an attention-based convolutional neural network. We evaluate the accuracy of the similarity detector on an evaluation set with 100 answers, and can get the 71% Mean Reciprocal Rank (MRR) score.

• Mycobiology
• /
• v.50 no.2
• /
• pp.121-131
• /
• 2022

The rare edible and medicinal fungus Antrodia cinnamomea has a substantial potential for development. In this study, Illumina HiSeq 2000 was used to sequence its transcriptome. The results were assembled de novo, and 66,589 unigenes with an N50 of 4413 bp were obtained. Compared with public databases, 6,061, 3,257, and 2,807 unigenes were annotated to the Non-Redundant, Gene Ontology, and Kyoto Encyclopedia of Genes and Genomes databases, respectively. The genes related to terpene biosynthesis in the mycelia of A. cinnamomea were analyzed, and acetyl CoA synthase (ACS2 and ACS4), hydroxymethylglutaryl CoA reductase (HMGR), farnesyl transferase (FTase), and squalene synthase (SQS) were found to be upregulated in XZJ (twig of C. camphora) and NZJ (twig of C. kanehirae). Moreover, ACS5 and 2,3-oxidized squalene cyclase (OCS) were highly expressed in NZJ, while heme IX farnesyl transferase (IX-FIT) and ACS3 were significantly expressed in XZJ. The differential expression of ACS1, ACS2, HMGR, IX-FIT, SQS, and OCS was confirmed by real-time quantitative reverse transcription PCR. This study provides a new concept for the additional exploration of the molecular regulatory mechanism of terpenoid biosynthesis and data for the biotechnology of terpenoid production.

• BMB Reports
• /
• v.56 no.3
• /
• pp.153-159
• /
• 2023

Neuronal differentiation is highly coordinated through a cascade of gene expression, mediated via interactions between trans-acting transcription factors and cis-regulatory elements of their target genes. However, the mechanisms of transcriptional regulation that determine neuronal cell-fate are not fully understood. Here, we show that the nuclear transcription factor Y (NF-Y) subunit, NFYA-1, is necessary and sufficient to express the flp-3 neuropeptide gene in the IL1 neurons of C. elegans. flp-3 expression is decreased in dorsal and lateral, but not ventral IL1s of nfya-1 mutants. The expression of another terminally differentiated gene, eat-4 vesicular glutamate transporter, is abolished, whereas the unc-8 DEG/ENaC gene and pan-neuronal genes are expressed normally in IL1s of nfya-1 mutants. nfya-1 is expressed in and acts in IL1s to regulate flp-3 and eat-4 expression. Ectopic expression of NFYA-1 drives the expression of flp-3 gene in other cell-types. Promoter analysis of IL1-expressed genes results in the identification of several cis-regulatory motifs which are necessary for IL1 expression, including a putative CCAAT-box located in the flp-3 promoter that NFYA-1 directly interacts with. NFYA-1 and NFYA-2, together with NFYB-1 and NFYC-1, exhibit partly or fully redundant roles in the regulation of flp-3 or unc-8 expression, respectively. Taken together, our data indicate that the NF-Y complex regulates neuronal subtype-specification via regulating a set of terminal-differentiation genes.