• Title/Summary/Keyword: red spot

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Antagonistic Effect of Lactobacillus sp. Strain KLF01 Against Plant Pathogenic Bacteria Ralstonia solanacearum (세균성 시들음병에 대한 식물성 유산균(Lactobacillus sp.)의 저해효과)

  • Shrestha, Anupama;Choi, Kyu-Up;Lim, Chun-Keun;Hur, Jang-Hyun;Cho, Sae-Youll
    • The Korean Journal of Pesticide Science
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    • v.13 no.1
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    • pp.45-53
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    • 2009
  • An antagonistic bacterial strain KLF01 was isolated from rhizosphere of tomato and identified to be Lactobacillus sp. by biochemical and genetic analysis. This strain showed antagonism against the used plant pathogenic bacteria like Ralstonia solanacearum, (bacterial wilt), Xanthomonas axonopodis pv. citri, (Citrus canker), Xanthomonas campestris pv. vesicatoria (Bacterial spot), Eriwinia pyrifoliae (Shoot-blight) and Eriwinia carotovora subsp. carotovora group (Potato scab) through agar well diffusion method. In planta test done by drench application of strain KLF01 $(4{\times}10^8 cfu/ml)$ into the experimental plot containing tomato (Solanum lycopersicum L.) cultivar 'Lokkusanmaru' and red pepper (Capsicum annuum L.) cultivar 'Buja' plants, in pot test post-inoculated with the plant pathogenic bacteria, R. solanacearum significantly reduced the disease severity, compared to the non-treated plants.

Quality Control of Ginseng Products(Part I) - The saponins isolated from ginseng roots and leaves - (인삼제품(人蔘製品)의 품질개량(品質改良)에 관(關)한 연구(硏究) (제일보(第一報)) - 인삼근(人蔘根) 및 엽(葉) Saponin의 비교연구(比較硏究) -)

  • Cho, Han-Ok;Cho, Sung-Hwan;Kim, Soo-Ja
    • Applied Biological Chemistry
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    • v.22 no.1
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    • pp.10-17
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    • 1979
  • The saponins isolated form the herb of Panax ginseng C.A. Meyer were investigated as compared with ginseng root saponins. By adopting DEAE cellulose ion exchange chromatography the pure saponins were isolated from Korean ginseng roots and leaves. The ginseng root and leaf saponins showed some differences in the pattern of the two-dimensional thin layer chromatogram. The ratio of panaxadiol to panaxatriol in the saponins was 1.7 in the roots and 3.5 in the leaves. Infra-red spectrum of ginseng leaf saponins isolated by liquid chromatography was identical with that of root saponins.

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Identication of Bean Yellow Mosaic Virus from Trifolium repens (흰 토끼풀에서 분리한 강낭콩황반모자이크 바이러스의 동정)

  • Ryu Gab Hee;Lee Soon Hyung;La Yong Joon
    • Korean Journal Plant Pathology
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    • v.2 no.1
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    • pp.48-52
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    • 1986
  • The virus isolated from white clover, Trifolium repens showing mosaic symptom was identified as bean yellow mosaic virus (BYMV) based on the host range, physical properties, aphid transmission, serology and morphology of the virus particles. Chenopodium amaranticolor and C. quinoa produced local lesions on the inoculated leaves and chlorotic spot on the upper leaves. Broad bean and cowpea produced local lesions on the inoculated leaves and mosaic with vein necrotic symptoms on the upper leaves. French bean showed vein necrosis on the inoculated leaves, yellow mosaic on the upper leaves and bud blight. The average size of virus particles was 740nm in length. The virus was also transmitted by Myzus persicae. The thermal inactivation point of the virus isolate was $60\;to\;65^{\circ}C$, the dilution end point $10^{-3}\;-\;10^{-4}$ and the longevity in vitro was 3 days Serological tests with the virus purified from Trifolium repens were positive to BYMV antiserum.

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Strawberry Virus Diseases Occurring in Korea, 2007-2008 (우리나라에서 발생하는 딸기 바이러스병(2007-2008))

  • Choi, Gug-Seoun;Lee, Jin-A;Cho, Jeom-Deog;Chung, Bong-Nam;Cho, In-Sook;Kim, Jeong-Soo
    • Research in Plant Disease
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    • v.15 no.1
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    • pp.8-12
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    • 2009
  • Virus disease surveys of strawberries cultivated and preserved as germplasm resources in Korea was conducted during 2007-2008. Virus detection was conducted by RT-PCR using total RNAs extracted from strawberry samples. We detected the infection with Strawberry mild yellow edge virus (SMYEV), Strawberry mottle virus (SMoV), Strawberry vein banding virus (SVBV) and Strawberry pallidosis associated virus (SPaV) while no infection with Strawberry crinkle virus (SCV), Strawberry necrotic shock virus (SNSV), Strawberry latent ring spot virus (SLRSV) and Arabis mosaic virus (ArMV) was observed. The infection rate of virus disease on 4 cultivars including Seolhyang, Maehyang, Gumhyang, and Dahong, bred in Korea, was 0.1, 1.9, 0, and 0%, respectively. Surprisingly, however, cultivar Red Peal introduced from Japan in 1997 revealed 48.3% virus infection rate. SMYEV, SMoV and SPaV were also identified in strawberries growing in the farm fields of Korea. In the field, however, SMYEV was the most predominant virus (97.4%) among those 3 identified viruses. SVBV was detected only in strawberry kept as a germplasm.

Seasonal survey on the respiratory diseases of slaughtered pigs in Jeonbuk, Korea (전북지역 도축돈 호흡기질병 조사)

  • Lim, Mi-Na;Kim, Chul-Min;Park, Young-Min;Song, Ju-Tae;Jin, Jae-Kwon;Cho, Hyun-Ung
    • Korean Journal of Veterinary Service
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    • v.39 no.4
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    • pp.231-237
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    • 2016
  • The present study was conducted to investigate the lesion of red internal organs in slaughtered pigs and provided assistant data for pig farms. During March to December 2015, a total of 1,160 lung samples out of 58 herds were collected randomly from pigs slaughtered in Jeonbuk province. In addition, 290 hilar lymph nodes from pig with pneumonic lung lesion (5 samples per herd) were screened for selected viral and bacterial pathogens. Gross lesions of lungs such as swine enzootic pneumonia (SEP), pleuritis, pleuropneumonia, pericarditis and liver white spots were examined. The overall prevalence of SEP was 64.3% (746/1,160). In the analysis of seasonal prevalence, there was an increase of occurrence during the spring months (287/400, 71.8%) and decrease during the fall months (93/200, 46.5%) among the whole herds. The mean number of SEP score per pig was $1.20{\pm}1.28$. The prevalence of pleuropneumonia, pleuritis, pericarditis, and milk spot was 25.5% (296/1,160), 44.1% (512/1,160), 3.8% (44/1,160) and 17.6% (204/1,160), respectively. The most frequent region with lung lesion was diaphragmatic lobes (left 17.1%, right 17.3%). In the detection of viral pathogens by PCR, porcine circovirus type2 (PCV2) was positive in 86.9% (252/290), while porcine reproductive and respiratory syndrome virus (PRRSV) was not detected, In the case of bacterial pathogens, 50 microorganisms were isolated by PCR and/or microbiological test. The most frequently isolated bacteria was Streptococcus suis (20, 34.4%), followed by Pasteurella multocida (17, 29.3%), Streptococcus spp. (11, 3.4%), Actinobacillus pleuropneumoniae (2, 8.9%).

Apple Intermediate Parent of Spur Type "Wonkyo Ga-Dangwagi 1" (사과 단과지성 중간모본 "원교 가-단과지 1호")

  • Heo, Seong;Hwang, Jeong Hwan;Shin, Il Sheob;Shin, Yong Uk;Kim, Ki Hong
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.543-546
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    • 2009
  • Intermediate parent "Wonkyo Ga-Dangwagi 1" (Malus domestica Borkh.) was released from Fruit Research Division, National Institute of Horticultural & Herbal Science, Korea in 2008. It was derived from the cross between "Starkrimson" and columnar type "McIntosh Wijcik" in 1992 after selection tests from 2000 to 2008. Trees are moderately vigorous and have spreading branching habit. It bears abundant flower buds on one-year-old branches and fruits mainly on spurs or short branches. Harvesting time of this variety is late September, 7 days later than that of "Hongro" in Suwon. The fruit is conical to narrow conical in shape and bright red in skin color. Fruit size is small with weight of 200~240g on an average and fruits have $14^{\circ}Bx$ soluble solid and medium acidity. It is moderately susceptible to alternaria leaf spot (Alternaria alternata). "Wonkyo Ga-Dangwagi 1" is a promising apple cultivar and will appeal to young people with good sugar-acid balance.

First Record of Bothus pantherinus (Bothidae, Pleuronectiformes) from Korea (한국산 둥글넙치과(Bothidae) 어류, Bothus pantherinus의 첫기록)

  • Si-Yeong Jeong;Jin-Koo Kim
    • Korean Journal of Ichthyology
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    • v.35 no.1
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    • pp.44-49
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    • 2023
  • A single specimen of the genus Bothus (family Bothidae) was collected for the first time at the intertidal zone of Moseulpo Port, Daejeong-eup, Seogwipo-si, Jejudo Island, on 14 August 2022. The specimen was identified as Bothus pantherinus in having following morphological traits: dark spots and ring-shaped patterns near eyes and the pectoral fins, one distinct spot in the center of the lateral line, 75 lateral line scales, and seven hourglass shaped supracranial pterygiophores. As a result of analyzing 603 bp of mitochondrial DNA COI sequences, our specimen was perfectly matched to those of B. pantherinus registered in NCBI. It has been known that the species is widely distributed throughout the Indo-Pacific Ocean from Red Sea to Hawaiian (32 degrees north to 32 degrees south), but this study revealed that its distribution expanded to the waters of Jejudo Island (33 degrees north), Korea. We propose its new Korean name "Beot-kkoch-mu-nui-dung-geul-neob-chi".

Comparison of Taste Compounds of Red Sea Bream, Rockfish and Flounders Differing in the Localities and Growing Conditions (산지 및 성장조건별 참돔, 조피볼락, 넙치의 정미성분에 대한 연구)

  • Kim, Hee-Yun;Shin, Jae-Wook;Park, Hee-Ok;Choi, Sung-Hee;Jang, Young-Mi;Lee, Soo-Oh
    • Korean Journal of Food Science and Technology
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    • v.32 no.3
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    • pp.550-563
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    • 2000
  • This study was carried out in order to elucidate the fundamental data on the taste compounds between wild and cultured fishes produced on Chungmu and Wando at the southern coast areas of Korea. For this purpose, the food components of cultured fishes such as red sea bream Pagnus major, Sebastes pachycephalus and flounder, Paralichthys olivaceus being spot lighted for the main sea fish, the staple food and high economic fish were investigated and compared with those of the wild ones. There was a little appreciable difference in the proximate compositions of all the species from localities between wild and cultured fishes. But according to the growing conditions, wild fishes were higher in moisture contents and lower in crude lipid content than those of cultured fishes and little difference was seen in protein and ash contents between the two. With regard to the nucleotides and their related compounds, i.e. ADP, IMP and inosine were detected but ATP and hypoxanathine were not from them. On the other hand, there were little difference in the total taste compounds of all the species from localities and the growing conditions between wild and cultured fishes. But all the species were higher in IMP content. The total of seventeen amino acids were detected in samples. The highly contents of glutamic acid, lysine, aspartic acid, proline, leucine, alanine, valine and alginine were showed and less low contents of cystein, histidine, methionine, tyrosine and phenylalanine were detected. The total amino acids of the others were much alike in that composition. Little difference was seen from localities and the growing conditions between wild and cultured ones. The free amino acids were much alike in that composition of all the species. There was little difference in the free amino acid compositions all the species from localities and the growing conditions between wild and cultured fishes. But taurine was dominant, showing from 39% to 65% of the free amino acid content and it is followed by hydroxyproline, lysine, alanine and glycine in other. There were differences in the organic acid compositions of all the species from localities and the growing conditions between wild and cultured fishes. In addition, cultured fishes were more abundant in the total organic acid compositions than those of wild ones.

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Characteristics of the Strains Selected from Crosses between Introduced Interspecific Hybrids and Cultivars in Hibiscus Species (종간교잡 유래 도입 무궁화와 국내 선발 품종과의 교잡에 의해 육성된 계통들의 특성)

  • Kang, Ho-Chul;Ha, Yoo-Mi;Kim, Dong-Yeob;Han, In Song;Noh, Kwang-Mo
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.1
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    • pp.55-63
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    • 2011
  • This study was carried out to develop new cultivars of Hibiscus species from crosses between introduced interspecific hybrids and cultivars in Hibiscus species. Fruit setting of interspecific crosses of Hibiscus strains was less than 10% and the number of seeds in the fruit was also in low level. Three individuals of specific flower and leaf characteristics were selected from crosses between introduced interspecific hybrid, 'Fujimusme'(♀), and H. syriacus 'Namwon'(♂) in 2004. A new strain, Hibiscus ${\times}$ 'W-26', was selected from the crossing of interspecific hybrid, 'Fujimusme'(♀), and H. syriacus 'Namwon'(♂), which had white flower and narrow separated petal. Hibiscus ${\times}$ 'WRB-2' was selected from the crossing of interspecific hybrid, 'Fujimusme'(♀), and H. syriacus 'Namwon'(♂), which had white flower and blue eye spot. Hibiscus ${\times}$ 'R-141' was selected from crosses between introduced interspecific hybrid, 'Shichisai'(♀) and H. syriacus 'Namwon'(♂), which had large flowers over 13 cm diameter and revealed tall tree type. Hibiscus ${\times}$ 'R-142' was selected from the crossing of interspecific hybrid, 'Shichisai'(♀), and H. syriacus 'Namwon'(♂), which had large flowers over 13 cm diameter and revealed tall tree type. The characteristics were succeded after grafting. Flower of 'R-142' had reddish violet color with red eye spot, whereas its parent had blue and purple flowers.

Effect of Cultivation Using Plastic-Film House on Yield and Quality of Ginseng in Paddy Field (논토양에서 비닐하우스를 이용한 재배방법이 인삼의 수량 및 품질에 미치는 영향)

  • Kim, Dong Won;Kim, Jong Yeob;You, Dong Hyun;Kim, Chang Su;Kim, Hee Jun;Park, Jong Suk;Kim, Jeong Man;Choi, Dong Chil;Oh, Nam Ki
    • Korean Journal of Medicinal Crop Science
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    • v.22 no.3
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    • pp.210-216
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    • 2014
  • The average and maximum temperature were $29.5^{\circ}C$ and $33.2^{\circ}C$ at 2:00 p.m. respectively, in the plastic-film house covered with shade net, and both of temperature were lower $0.6^{\circ}C$ and $1.3^{\circ}C$ than those of conventional shade. Light transmittance was 14% in the plastic-film house, while 9.9% in conventional shade during growing season from May to October. Withering time of aboveground part was on October 3rd in conventional shade with 60% of withering leaf, while it was on November 10th with 3.7% of withering leaf in the plastic-film house, about 40 days longer survival. The main disease incidence were 15% of anthracnose, 17% of leaf spot, 5% of phytophthora blight and 3% of gray mold in the conventional shade, while 0 ~ 0.1% disease incidence and 95% of emergence rate in the plastic-film house. The growth in the aboveground and underground part of ginseng was totally better, particularly characteristics affecting yield such as root length, main root length and diameter in the plastic-film house. The fresh weight was increased by 128% compared to the conventional shade and harvested roots per $3.3m^2$ were 36 roots in the conventional shade and 58 roots in the plastic-film house and futhermore yield per $3.3m^2$ was increased by 216% compared to the conventional shade. As covering materials, the rice straw in the plastic-film house was excellent. The ginsenoside contents affecting the quality of ginseng were higher in the plastic-film house indicating 0.333% of Rg1, 0.672% of Rb1, 0.730% of Rc and rate of red rusty root was less than 4.0 ~ 6.1%. Above the results, the quality of ginseng grown in the plastic-film house covered with shade net was improved than that of the conventional shade.