• Biotechnology and Bioprocess Engineering:BBE
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• 제10권3호
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• pp.262-269
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• 2005

Many researchers have employed cryopreserved amniotic membrane (CAM) in the treatment of a severely damaged cornea, using corneal epithelial cells cultured on an amniotic membrane (AM). In this study, two Teflon rings were made for culturing the cells on the LAM and CAM, and were then used to support the AM, which is referred to in this paper as an Ahn's AM supporter. The primary corneal epithelial cells were obtained from the limbus, using an ex-plantation method. The corneal epithelium could be reconstructed by culturing the third­passage corneal epithelial cells on the AM. A lyophilized amniotic membrane (LAM) has a higher rate of graft take, a longer shelf life, is easier to store, and safer, due to gamma irradiation, than a (AM. The corneal epithelium reconstructed on the LAM and (AM, supported by the two­Teflon rings, was similar to normal corneal epithelium. However, the advantages of the LAM over that of the (AM make the former more useful. The reconstruction model of the corneal epithelium, using AM, is considered as a good in vitro model for transplantation of cornel epithelium into patients with a severely damaged cornea.

• KSBB Journal
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• 제20권4호
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• pp.305-310
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• 2005

동결건조 양막과 자체 제작한 양막 지지체를 이용한 동적 배양법을 통하여 토끼 각막 상피층의 재구성 효과를 검토하였다. 각막 상피세포는 토끼 각막 윤부로부터 분리 배양 후 동결보존하여 사용하였고, 세포증식은 10계대까지 가능하였다. 동적배양을 한 경우가 정치배양보다 기저층이 잘 형성되었고, 세포 증식과 분화를 촉진하여 치밀한 상피층을 형성하였다. 이렇게 재구성된 각막 상피층은 심한 각막 손상을 입은 환자의 조직 이식에 좋은 생체외 모델이 될 것으로 기대된다.

• 대한수의학회지
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• 제52권3호
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• pp.157-162
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• 2012

Corneal injury is very common clinical condition in veterinary medicine and delayed or incomplete corneal healing has the potential of vision loss due to the loss of corneal transparency. For the reconstruction of corneal epithelium, tissue graft and cell transplantation have been prosperously investigated. The purpose of this study was to evaluate the clinical value and short-term safety of application of cultured allogenic mesenchymal stem cells (MSCs) in the treatment of canine experimental corneal defect. Corneal defects were surgically generated in the central corneas of healthy beagle dogs and cultured canine allogenic MSCs were transplanted via subconjunctival injection. Although mean healing time, the rate of epithelial regeneration, and the degree of corneal transparency were not significantly improved after MSC transplantation, significant immune reaction or incompatibility reaction was not detected except transient local irritation. These results propose the possibility of MSC application as a new regenerative medicine in canine ocular disorders.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.393-394
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• 2005