• Research in Plant Disease
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• v.29 no.4
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• pp.452-458
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• 2023

In the Goesan region, located in Chungcheongbuk-do, Korea, a significant outbreak of soft rot infections was documented in August 2021, affecting fruits of Momordica charantia, commonly known as bitter melon or bitter gourd. The symptoms included a noticeable transition to yellowing in the affected fruits, eventually leading to their collapse. The bacterial strain KNUB-09-21 was isolated from the diseased fruits. Molecular analysis, using the sequences of the 16S rRNA region and three housekeeping genes (dnaX, recA, and leuS), along with the results of compound utilization in the API ID 32 GN system, provide strong evidence for the identification of the isolate KNUB-09-21 as Pectobacterium brasiliense. The pathogenicity of strain KNUB-09-21 on M. charantia was confirmed through a controlled inoculation test. Within two days, inoculated fruits displayed soft rot symptoms closely resembling those observed in naturally affected fruits. This is the first report of soft rot on M. charantia in Korea.

• Research in Plant Disease
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• v.30 no.1
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• pp.88-94
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• 2024

In July 2021, symptoms of soft rot were observed on the stems of Ficus carica in Yeongam, Jeollanamdo, Korea. To accurately diagnose the cause, infected stem was collected and bacterial strain was isolated. Among these, the pathogenic strain KNUB-08-21 was identified as Pectobacterium aroidearum through 16S rRNA gene sequencing and phylogenetic analysis based on the concatenated sequences of the dnaX, leuS, and recA genes. The affiliation of the isolate with this bacterial species was also confirmed by its biochemical characteristics obtained using API ID 32 GN system. Artificial inoculation confirmed the strain's pathogenicity in figs, causing significant damage to both stems and fruits. To our knowledge, this is the first report of P. aroidearum causing soft rot disease in F. carica in Korea.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.85-85
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• 2015

현재 태양광에너지 시장은 해마다 빠른 속도로 성장하는 추세이며 50 GWp/년 이상의 시장으로 변화하였고 앞으로도 전체적인 성장세는 지속 유지될 것으로 판단된다. 하지만 이와 아울러 각 Value chain 별로 많은 기업들이 생겨나게되어 각각의 기업들이 제품을 고효율 저가화 함으로서 경쟁력을 확보하기 위해 많은 노력들이 기울이고 있으며 본 강연에서는 이러한 측면에서 태양광 에너지의 분야별 고효율/저가화를 위한 기술동향을 살펴보고자 한다. 태양광 산업은 아직은 다소 높은 발전단가로 인해 일부 정부의 지원이나 정책에 의해 산업의 규모가 결정되게 되는데 주요한 지원제도는 RPS 제도와 FIT 제도가 있으며 우리나라는 초기 FIT 제도로 국가에서 태양광에서 생산된 전기를 높은 가격에 사주었으나 근래에들어 RPS 제도를 운영하게 되었으며 매전을 하면서 SMP에 준하는 수익을 창출하고 이와 아울러 REC 를 확보하여 확보된 REC 단가에 의해 추가적인 수익을 창출하는 구조의 발전사업이 진행되고 있다. 그리고 RPS나 FIT와 같은 정부의 지원없이도 발전단가의 경쟁력을 확보하는 시점을 그리드패러티라고 하며 이는 매우 중요한 의미를 갖는다. 태양광의 저가화는 그리드패러티 달성을 확보하기 위해 필수적으로 필요한 사안이며 앞으로도 이러한 저가화 / 고효율화 기술노력은 계속 진행될 것으로 판단된다. 우선 소재의 가격을 줄이기 위해 웨이퍼의 두께가 점점 박형화 되어가고 박형화 되면서도 안정적인 공정수율 및 효율을 향상시키기 위한 기술개발이 진행되고 있으며 Cell 분야에 있어서도 고효율을 위한 다양한 Texturing 기술 및 패시베이션 기술의 개발이 이루어 지고 있으며 고효율 컨셉의 MRT cell, Back contact cell 등 고효율 구조의 cell의 양산을 진행하고 있는 등 최근 n-type 기반의 고효율 cell 기술이 활발하게 양산화 검토가 이루어 지고 있다. 모듈 분야에 있어서는 저가/고효율화와 아울러 제품의 신뢰성 확보가 무엇보다도 중요하게 다루어 지고 있으며 이는 모듈이 최소 25년 이상 Field 에서 운용되어 수익창출이 가능해야 하므로 가장 중요한 요소중에 하나라고 할 수 있다. 신뢰성 측면에서 중요하게 다루어 지고 있는 것 중 하나가 PID 저감을 위한 노력이며 이와 관련된 각 소재의 개발이 가장 활발하게 진행되고 있으며 이와 아울러 장수명을 보장하기 위한 내구성이 겸비된 봉지재의 개발 또한 많은 관심을 불러 일으키고 있다. 저가/고효율화를 위해 CTM loss를 줄이기 위한 다양한 시도가 이루어지고 있으며 특수 형태의 리본으로 빛의 흡수를 증가시키거나 컨택저항을 최소화 하기위한 소재의 개발이 이루어 지고 있다. 태양광 시스템 분야의 경우 발전량과 수익창출에 있어 직접적인 영향을 미치는 분야로서 전체 시스템의 loss 를 줄이고 최적의 환경에서 최대한의 발전량을 확보하기 위한 array 설계 및 운용기술이 활발하게 개발되고 있으며 시스템에서의 loss를 줄여줄수 있는 마이크로 인버터나 multi string 인버터의 적용도 이루어 지고 있으나 저가화를 위한 추가적인 노력이 필요한 상황이다. 본 강연의 마지막으로 이러한 노력들의 산물인 특수 태양광 제품 및 시스템의 기술동향에 대해 살펴하고자 한다. 사막은 전체면적의 1/3을 차지할 정도로 넓은 면적을 자랑하지만 밤과 낮의 기온차 그리고 계통 선로의 부재 등 적용하기 어려운 환경적인 제약도 함께 존재하며 이러한 문제를 해결하기 위한 방안에 대해 살펴보고 최근 Hot issue 중의 하나인 수상 태양광 시스템의 장, 단점과 기술적 특성 등을 살펴보고자 한다.

• Journal of the Korea Organic Resources Recycling Association
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• v.22 no.1
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• pp.11-19
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• 2014

A study on combustion characteristic and evaluation of cost benefit were carried out using dried sewage sludge to evaluate co-combustion with coal in a coal-fired power plant. In the result of proximate analysis, sewage sludge has 78.09%, 79.65% of moisture content in A STP(Sewage Treatment Plant) and B STP, respectively. The dried sewage sludges show 0.12, 0.14 of fuel ratio value, respectively and over 30,000kcal/kg of combustible index. It means that the dried sewage sludges needs to reform from the results of fuel ratio and combustible index. As a results of the economical benefit evaluation of dried sewage sludge, about 73.4 million won through using the dried sewage sludges instead of coal and 56.4 million won through REC(Renewable Energy Certificate) cost were saved, respectively. On the other hand, it occurs 4.2 million won of additional cost related to ash disposal and 2.6 million won of investment/operation cost for co-combustion facility. In conclusion, co-combustion of dried sewage sludges with coal in a coal-fired power plant saves about 123 million won. However, it needs to consider for power supply to produce dried sewage sludges and opportunity cost for environmental pollution and so on to evaluate more reasonable benefit of co-combustion.

• Journal of Chest Surgery
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• v.25 no.1
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• pp.1-16
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• 1992

The quantitatively measured local myocardial perfusion rates with microspheres are used as an objective indicator of even distribution of cardioplegic solution, and the efficacy of the retrograde right atrial route of cardioplegia is evaluated in hearts with various levels of coronary arterial obstruction. After initial antegrade cardioplegia under the median sternotomy and aortic cannulation, 60 hearts from anesthetized New Zealand white rabbits are divided in random order as normal group [ligated left main coronary artery ; MA, MR] and diagonal group [ligated proximal diagonal artery ; LA, LR]. Half of each group [N=10] are perfused with antegrade cardioplegia[A] under the pressure of 100 cmH2O and the other half with retrograde right atrial route[R] under the pressure of 60 cmH2O[St. Thomas cardioplegic solution mixed with measured amount of microspheres]. The myocardium is subdivided into segments as A[atria], RV[right ventricle]. S[septum], LV[normally perfused left ventricular free wall], ROI[ischemic myocardium of left ventricular free wall]. LV and RQI are further divided into N[subendocardium] and P[subepicardium]. The resulting local myocardial perfusion rates and N /P of each group are compared with Wilcoxon rank sum test. The weight of the hearts is 5.94$\pm$0.66g, and there are no statistically significant dif-ferences[p>0.05, ANOVA] between six compared group. The mean flow rate[F: ml /g / min] of MR group is comparable with MA group[p>0.05], but in N and L group, there are significantly depressed F with right atrial route of cardioplegia, which means elevated perfusion resistance with this route. In spite of no significant differences in delivered doses of microsphere[DEL] between compared groups[p>0.05, ANOVA], there are significantly depressed REC and NF in hearts with right atrial cardioplegia which suggests increased requirement of cardioplegic solution with this route. The interventricular septum shows poor perfusion with right atrial route of cardioplegia without obstruction of supplying coronary arteries. But, with obstruction of coronary artery supplying septum as in M group, the flow rate is superior with right atrial route of infusion. The left ventricular free wall perfusion rates of every RQI with R route are superior to that of A route[p<0.05]. But, in LV segments, there are unfavorable effects of right atrial cardioplegia in L group, although the subendocardial perfusion is well maintained in N group. The LV free wall of left main group shows depressed perfusion rates with antegrade route as compared with RQI segments of diagonal group. But, by contraries, there are increased perfusion rates and superior N /P ratio with retrograde right atrial route. It implies more effective perfusion with right atrial route of cardioplegia in more proximal coronary arterial obstruction[i.e., M group as compared with L group]. As a conclusion, all region of ischemia have superior perfusion rates with right atrial car-dioplegia as compared with antegrade route, and especially excellent results can be obtained in hearts with more proximal obstruction of coronary arteries which would otherwise result in more severe ischemic damage. But, the depressed perfusion rates of the segments with normal coronary artery in hearts with coronary arterial obstruction may be a problem of concern with right atrial cardioplegia and needs solution.

• Journal of Microbiology and Biotechnology
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• v.27 no.1
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• pp.9-18
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• 2017

Nine bacilli with fibrinolytic activities were isolated from doenjang, a traditional Korean fermented soy food. Among them, RSB34 showed the strongest activity and was identified as Bacillus amyloliquefaciens by 16S rRNA and recA gene sequencing. During growth on LB up to 96 h, RSB34 showed the highest fibrinolytic activity ($83.23mU/{\mu}l$) at 48 h. Three bands of 23, 27, and 42 kDa in size were observed when the culture supernatant was analyzed by SDS-PAGE and 27 and 42 kDa bands by fibrin zymography. The gene encoding the 27 kDa fibrinolytic enzyme AprE34 was cloned by PCR. BLAST analyses confirmed that the gene was a homolog to genes encoding AprE-type proteases. aprE34 was overexpressed in Escherichia coli BL21(DE3) using pET26b(+). Recombinant AprE34 was purified and examined for its properties. The $K_m$ and $V_{max}$ values of recombinant AprE34 were $0.131{\pm}0.026mM$ and $16.551{\pm}0.316{\mu}M/l/min$, respectively, when measured using an artificial substrate, N-succinyl-ala-ala-pro-phe-p-nitroanilide. aprE34 was overexpressed in B. subtilis WB600 using pHY300PLK. B. subtilis transformants harboring pHYRSB34 (pHY300PLK with aprE34) showed higher fibrinolytic activity than B. amyloliquefaciens RSB34.

• Resources Recycling
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• v.5 no.1
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• pp.21-28
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• 1996

Burmng method tn Bnidi-ied beds 101 the effectlvr reclamation of Sunn fi,uadry sand war u~ed lather tl~an rncchan1c;ll or p~~cumatiace thodq. which are co~nmonlyu scd in foundry factoricr in Kojcu In olrlcr to rcrnuvc hlc;~ns and ha~doersi ~nm foundry sand sndaces in the fluidized bcd, the lnnsl sffccuve lcmpemture mge was h00-70W. Sulbcr slab~l~liyn dca and comliresslvc streugtli oL rccla~mcd sand recyclcd by the burning luctllod wcre lalgcr dao those of rcclalmcd a n d by lllc pncumatlc mctllod. Tile energy cost IOIt he bummy mctliod was cstimalcd al SlZ0011'on oi llie iccl;~~rncds md. The coql l~ductlon in pul.chasing nzw s;od as well as the prevenllon or cnv~conmcnl.il contamlnallnn could hc hmefitcrl hy usl~lg Ihc rec1;~rnnl~oonf liisndry siod ~ecycledb y the method rleieloped in thls sludy

• Research in Plant Disease
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• v.28 no.3
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• pp.172-178
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• 2022

The graft cactus (Gymnocalycium mihanovichii) continues to be exported to more than 20 countries worldwide. In April 2021, typical bacterial symptoms of soft rot were observed in the graft cactus (cv. Yeonbit) in Goyang, Gyeonggi-do, Korea, resulting in economic losses in cactus production. The stems turned dark brown and the flowers were covered with black rot. The bacterial strain designated as KNUB-01-21 was isolated from infected stems and flowers. The results of the morphological and biochemical tests of the isolate were similar to those of Pectobacterium brasiliense. For molecular analysis, the 16S rRNA region and three housekeeping genes (dnaX, leuS, and recA) of the strain KNUB-01-21 were amplified. Based on the results of the molecular analysis and morphological and biochemical tests, KNUB-01-21 was identified as P. brasiliense. The pathogenicity of KNUB-01-21 on graft cactus was confirmed by an inoculation test. Artificial inoculation using P. brasiliense KNUB-01-21 produced soft rot symptoms on the grafted cactus, and the same bacterium was re-isolated and re-identified. This is the first report of P. brasiliense causing soft rot in graft cactus in Korea.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 2001.05a
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• pp.162-168
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• 2001

성인의 정서에 따른 심혈관계 반응의 변화에 대한 연구는 많이 있으나, 아동 정서에 따른 심혈관계 반응에 대한 연구는 거의 없는 실정이다. 본 연구에서는 본 연구실에서 개발한 자극을 이용하여 유발된 정서에 따른 심혈관계 반응의 차이를 규명하고자 한다. 복합자극으로 구성된 아동용 정서유발 프로토콜을 사용하여 아동에게 다섯가지 정서(기쁨, 슬픔, 분노, 스트레스, 무료함)를 유발시키는 동안 심혈관계 반응(ECG, PPG)을 측정하였다. 실험참여 아동은 만 5세에서 9세 사이의 아동으로 모두 141명이며, 분석에는 132명 (학령전 남: 30/ 학령전 여: 33/ 학령후 남:37, 학령후 여:32)의 데이터가 활용되었다. 분석에 사용하나 심혈관계 생리반응 변수는 심박률(HR), 호흡주기 관련 심박률변화 (RSA), 심박률분산(HRV), 심박률분산의 고주파수성분 (HFHRV)과 저주파수성분 (LF HRV), 혈류량 (FPV)이었다. 이 변수들 중 심박률, R파 간 간격을 기준으로 한 심박률변산의 고주파수 성분과 저주파수 성분, 그리고 혈류량에서 정서에 따라 유의미한 차이가 나타났다. 심박률은 기쁨, 슬픔을 스트레스나 무료함과 구분할 수 있고, 분노를 다른 정서들과 구분하는 것으로 나타났다. 혈류량 역시 분노와 다른 정서들 간에 유의미한 차이를 보였다. 고주파수 성분은 무료함을 나머지 정서들과, 저주파수 성분은 슬픔, 스트레스를 무료함과 구별할 수 있었다. 정서에 따라서는, 기쁨과 분노 유발정서에서는 심박률과 혈류량이 감소하였고, 슬픔은 심박률과 저주파수성분이 감소하였으며, 스트레스는 독특하게 심박률이 감소하였다. 그리고 무료함에서는 고주파수성분과 저주파수성분은 증가하고 혈류량은 감소하는 특징적인 양상을 보였다. 결론적으로 본 연구는 심혈관계 반응을 이용하여 아동정서를 구분하는 것이 가능함을 밝혔다.공을 제외한 BH-2, BH-3호공에서의 이방성 투수량계수텐서값은 이 논문의 가정인 대수층이 이방성이고 균질하다는 가정에 일치하지 않았다. 따라서 원형좌표계에 도시하였을 때 다른 공에 비해서 비균질성이 심한 결과로 인해 이방성타원체 에서 많이 벗어남을 보였다. 이로인해 3개의 공 BH-1, BH-4, BH-5호공을 사용하여 대수층의 이방성을 분석하여 보았다. BH-1호공에서의 $T_{{\varepsilon}{\varepsilon}}$는 171.90 $m^2$/day, $T_{ηη}$는 71.0l $m^2$/day이고 주 텐서방향은 Nl5.39$^{\circ}$ E로 나타났다. BH-4호공의 $T_{{\varepsilon}{\varepsilon}}$는 268.20 $m^2$/day, $T_{ηη}$는 28.75 $m^2$/day이고 주 텐서방향은 N7.55$^{\circ}$E이며 BH-5호공에서의 $T_{{\varepsilon}{\varepsilon}}$는 168.40 $m^2$/day, $T_{ηη}$는 66.80 $m^2$/day이고 주 텐서방향은 N76.59$^{\circ}$E로 나타났다. 이처럼 연구지역에서의 각각의 공에 대한 투수량계수텐서는 서로 다르게 나타났으며 이에 따른 주 텐서방향도 서로 다름을 알 수 있다.. Targeting a 10% recycling rate for municipal waste in 2001. EPA plans to research and develop new rec

• Korean Journal of Animal Reproduction
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• v.26 no.3
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• pp.299-308
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• 2002

In horse, a single gene encodes both eCG and eLH $\beta$ subunits. The difference between eCG and eLH lies in the structure of their glycoresidues, which are both sialylated and sulfated in LH and sialylated in CG eCG consists of highly glycosyiated $\alpha$- and $\beta$-subunits and is an unique member of the gonadotropin family because it elicits response characteristics of both FSH and LH in other species than the horse. This dual activity of eCG in heterologous species is of fundamental interest to the study of gonadotropin structure-function relationships and the understanding of the molecular bases of the specific interactions of these hormones with their receptors. Thus, eCG is a dintinct molecule from the view points of its biological function and glycoresidue structures. The oligosaccharide at Asn 56 of the $\alpha$-subunit plays an indispensable role, whereas the carboxyl-terminal extension of the eCG $\beta$-subunit with its associated O-linked oligosaccharides is not improtant for, the in vitro LH-like activity of eCG. In contrast, both N- and O-linked oligosaccharides play important roles for FSH-like activity and increase FSH-like activity by removal of N- and O-linked oligosaccharides. Therefore, the dual LH- and FSH-like activities of eCG can be clearly separated by removal of either the N-linked oligosaccharide on the $\alpha$-subunit or CTP-associated O-linked oligosaccharides from its $\beta$-subunit. The glycoresidues seem to play crucial roles fer biological activities. The tethered-eCG was effciently secreted and showed similar LH-like activity to the dimeric eCG $\alpha$/ $\beta$ and native eCG. FSH-like activity of the tethered-eCG was also shown similarly in comparison with the native and wild type eCG $\alpha$/ $\beta$. Our data for the first time suggest that the tethered-eCG can be expressed efficiently and the produced product by the CHO-Kl cells is fully LH- and FSH-like activities in rat in vitro bioassay system. Our results also suggest that this molecular can imply particular models ot FSH-like activity not LH-like activity in the eCG. Taken together, these data indicate that the constructs of tethered molecule will be useful in the study of mutants that affect subunit association and/or secretion.