• Journal of Aquaculture
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• v.1 no.1
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• pp.13-24
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• 1988

In order to investigate a reasonable rearing density and the possibility of high-density rearing, flounder, Paralichthys olivaceus, juveniles of 2.53$\pm$0.24 cm in total length and 1.12$\pm$0.12 cm in body height were used in this study. The initial rearing density of them was 10 (D10), 20 (D20), 30 (D30) and 40 (D40) individuals per 137.75 $cm^2$ of bottom area, respectively. Ranges of water temperature and specific gravity during the rearing period of 65 days were $21.0\~27.0^{\circ}C$ and 1.024$\~$1.026, respectively, showing relatively higher values than that of natural sea water. Dissolved oxygen during the rearing period was 5.4$\~$7.5 ml/$\iota$ and inorganic nitrate was 0.07$\~$0.48 ppm in $NH_4^+-N$, 0.006$\~$0.33 ppm in $NO_2^{-}-N$ and 3.89$\~$34.06 ppm in $NO_3^{-}-N$. Growth in total length and body height of the juveniles in four rearing density at the end of the experiment was 8.17$\pm$0.80 em and 4.16$\pm$0.39 em, the highest in D20 and 7.72$\pm$0.40 cm and 3.94$\pm$0.21 cm, the lowest in D10. Significant differences, however, were not recognized between the slope values of growth regressions in four rearing density. Slope values of the relative growth between total length and body height of the juveniles in four rearing density were 0.5346, the highest in D10 and 0.5165, the lowest in D30, but there were no significant differences in those values. Survival rate of juveniles at the end of the experiment was $90\%$ in D10, D20 and D30, but that of D40 was $75\%$. The relationship between total length X body height (X) and body surface area of ocular side (Y) to calculate the rate of Y to bottom area in rearing tank (covering rate) as an indicator of rearing density was expressed by a linear regression, Y=0.5994X+0.1840. Covering rate in four rearing density at the end of the experiment was ranged 1.2$\~$4.1 times. Judging from the covering rate above 4 times, it seems to be possible rearing the flounder juveniles in high-density.

• Journal of Aquaculture
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• v.20 no.3
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• pp.178-183
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• 2007

We investigated the effect of salinity decrease, food supply and color of the rearing tank on the growth and survival of Amphiprion polymnus larvae. White tanks had the highest survival rate, followed by transparency, black and blue tanks(P<0.05). The transparency tank had the best growth, followed by black tank, white and blue tanks(P<0.05). Daily food intakes of larvae with $5.1{\sim}10.0mm$ in total length were $36.8{\sim}429.3$ Artemia nauplii. When salinity was lowered 5 psu per week from 32 psu, all the larvae died under salinity condition 17 psu. In lowered 2 psu per 3 days, its all died at 16 psu.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.21 no.5
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• pp.288-291
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• 1988

We have veen already reported the distribution of PSP of bivalve mollusca in southern coast of Korea and also analyzed their characteristics. The purpose of this study was to develop detoxification method for PSP infested sea mussel, Mytilus edulis, by rearing methods or processing treatments. There was no significant detoxification effect when the PSP infested sea mussel was reared in a tank with water recirculation system, but the toxicity of sea mussel rapidly decreased during the rearing time in a water flow system with filtered water. The detoxification rate of PSP during the rearing for 5 days in a water flow system tank with $15-17^{\circ}C$ of sea water was $94\%$ in case of high toxic sample with more than $2,600{\mu}g/100g$ and about $40\%$ in case of low toxic sample with less than $100{\mu}g/100g$. The toxicity of PSP extracted from the sample with 0.1N/ HCl solution was about 2-5 times higher than that extracted with distilled water. When sea mussel contained $100-150{\mu}g-PSP$ per 100g of edible meat was boiled for 30 min with tap water, the toxicity was destroyed as the level of PSP undetected by mouse assay. We can suggest that boiling of sea mussel with tap water was one of the most significant detoxification methods, but it was not enough to be safe in case of extremely high intoxicated sea mussel with PSP. For example, the digestive gland of sea mussel contained more than $9593{\mu}g/100g$ was heated in a can with tap water at $116^{\circ}C$ for 65 min. the residual PSP was more than $170{\mu}g$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.21 no.5
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• pp.297-302
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• 1988

We have veen already reported the distribution of PSP of bivalve mollusca in southern coast of Korea and also analyzed their characteristics. The purpose of this study was to develop detoxification method for PSP infested sea mussel, Mytilus edulis, by rearing methods or processing treatments. There was no significant detoxification effect when the PSP infested sea mussel was reared in a tank with water recirculation system, but the toxicity of sea mussel rapidly decreased during the rearing time in a water flow system with filtered water. The detoxification rate of PSP during the rearing for 5 days in a water flow system tank with $15-17^{\circ}C$ of sea water was $94\%$ in case of high toxic sample with more than $2,600{\mu}g/100g$ and about $40\%$ in case of low toxic sample with less than $100{\mu}g/100g$. The toxicity of PSP extracted from the sample with 0.1N/ HCl solution was about 2-5 times higher than that extracted with distilled water. When sea mussel contained $100-150{\mu}g-PSP$ per 100g of edible meat was boiled for 30 min with tap water, the toxicity was destroyed as the level of PSP undetected by mouse assay. We can suggest that boiling of sea mussel with tap water was one of the most significant detoxification methods, but it was not enough to be safe in case of extremely high intoxicated sea mussel with PSP. For example, the digestive gland of sea mussel contained more than $9593{\mu}g/100g$ was heated in a can with tap water at $116^{\circ}C$ for 65 min. the residual PSP was more than $170{\mu}g$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.1
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• pp.27-34
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• 2002

The performances of fish culture were evaluated in the integrated recirculating aquaculture system consisting of a double drain type rearing tank ($2.5 m^3$), a sedimentation tank, a floating bead filter, a foam separator and a rotating biological contactor. A system was stocked with nile tilapia at an initial rearing densities of $5\%$ (A), $5\%$ (B) and $7\%$ (C) oyer 30 days. Feed coefficient was 1.62, 1,79 and 1.80 and average daily growth rate was $0.452\%$, 0.u5% and $0.423\%$, respectively. The level of TAN was maintained below $1 g/m^3$ for $5\%$ of initial rearing density and $2-4 g/m^3$ for $7\%$. The dissolved oxygen level ($2-4 g/m^3$) was not optimum but should not be inhibitory to fish growth. The total suspended solid was completely removed during overall experimental period.

• Journal of fish pathology
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• v.16 no.1
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• pp.13-21
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• 2003

The infection characteristics with scuticociliates at on-land rearing farms and hatcheries of flounder, Paralithys olivaceus was investigated during the year of 2001 by juvenile infection routes. When culture tanks for living food organisms such as chlorella, rotifer, and Artemia were searched, scuticocilates were detected both in live and dead rotifer, and at the dregs of culture tank bottoms at almost hatcheries. When rotifer infected with scuticocilates fed on fish larvae, lots of scuticocilate were inhabited at the bottom of fry rearing tanks. After feeding on scuticocilates-infected rotifer on fish larvae, first infection was detected at 10 days after bottom dwelling or 40 days old after hatching. By histopathological examination we confirmed the infection route of eyeball or brain contamination was that the ciliate worms digged through mouth and front part of the dosal fin cuticle, transferred into eyeball along the epithelium and muscle tissue, and reached finally into brain by the muscle and nerve tissue. The infection of internal organs was clarified into two routes. The first route was started from the infection at ventral and anal fin rays by the worms, and reached at the anus and rectum through the epithelium and muscle tissue. The second route was initiated from the infection at urinary organ and reached into the rectum epithelium cells, inner wall of intestine, abdominal cavity, pancreas, kidney, and pancreas. At seed production farms where fish larvae fed on scuticocilate-free rotifer, the worms were not detected not only at the food organisms culture tanks and juvenile rearing tanks but also larval flounder less than 7cm in total length.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.2
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• pp.140-145
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• 2002

The performances of a parallel current air driven type foam separator were evaluated in the pilot-scale recirculating aquaculture system. The system was stocked with Nile tilapia (Oreochromis niloticus) at an initial rearing densities of $2\%$, $5\%$ and $7\%$ of water volume of rearing tank and reared for 15, 30, 35 days, respectively. The condensated volume of effluent foam was increasing with increased rearing density. As increasing rearing density from $2\%$ to $7\%$, the protein concentration in rearing tank was increased from 16.6 g/$m^3$ to 21,9 g/$m^3$ and the removal amount of protein through foam separator as increased from 0.99 g/day to 2.5 g/day. But protein concentration ratio in the foam was decreased from 3.2 to 1.9. Changes of the removal amount and the concentration ratios of total suspended solid (TSS) and chemical oxygen demand (COD_cr.) were similar to proteins. The highest concentration ratios of TSS and COD_cr. were 10.2 and 8.4 at 2$2\%$ of rearing density.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.18 no.6
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• pp.563-570
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• 1985

The main purpose of this study is to evaluate a revolving plate-type biofilter system for mass culture of eels (Anguilla japonica) based on the experimental rearing for 199 days. Water quality, growth efficiency of fish and effects of fish disease control were critically evaluated. The experiment was conducted in two different units, each unit consisting of a cement tank containing $20m^3$ of water. In unit A, a biofilter which includes 400 rotating undulated P. V. C. plates being 70 cm in diameter which rotates at 6 rpm and also 400 undulated P. V. C. plates fixed in the settling chamber of an area of $66{\times}62cm$. Water was continuously passed through the filter at a rate of 260 l/min., and supplemental water was added to the fish tank at a rate of $4m^3$ a day. In unit B, the biofilter has 400 P. V. C. plates being $66{\times}62cm$ each was installed in the settling tank. The results gained from the experimental rearing for 199 days from April 21, 1984 to November 5, 1984 are as follows. In the growth experiment, the weight of fish in unit A increased from 3.0 kg to 815.6 kg, while in unit B, from 3.0kg to 416.0kg. During the period of the experiment, in the both units the fish grew at an acceptable rate at the temperature at which they were held. Observing every aspect of eel culture, including growth rate, disease control and water quality, unit A appears to have adventages over unit B, which makes it particuraly attractive in intensive recirculating fish culture system. It was further observed that certain parasites such as Trichodina sp. and Costia sp. could easily be controled by appling 4 ppm of $KMNO_4$.

• Journal of Aquaculture
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• v.22 no.1
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• pp.118-121
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• 2009

This experiment was conducted to evaluate the effects of different substrates in rearing tank on growth and body composition of juvenile sea cucumber Apostichophus japonicus. Sea cucumber (average weight 3.6) were randomly distributed at a density of 10 juvenile per tank into nine tanks providing three different substrates including free-substrates, sand and wave-shaped plastic sheet as (shelter). Juveniles were fed with a commercial feed once in every 2 days for 8 weeks. At the end of the experiment, survival of sea cucumber was not affected by the presence of substrates (P>0.05). Weight gain and specific growth rate of sea cucumber reared in the tanks with sand were significantly higher than those of sea cucumber reared in the tanks with shelter and without substrate (P<0.05). Crude protein and crude lipid of whole body in sea cucumber reared in free-substrate tanks were significantly higher than those of juvenile reared in the tanks with other substrates (P<0.05). Higher ash content was obtained in sea cucumber reared in the tanks with sand compared to that of juvenile reared in the tanks with other substrates (P<0.05). The results of this study suggest that growth and proximate composition of whole body of sea cucumber was significantly affected by substrates. Among materials tested, sand may be a proper substrate for sea cucumber culture.

• Fisheries and Aquatic Sciences
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• v.19 no.4
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• pp.15.1-15.5
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• 2016

Apparent digestibility coefficients (ADCs) of dry matter, crude protein, crude lipid, nitrogen-free extract, and energy in selected carbohydrate sources including wheat flour (WF), ${\alpha}-potato$ starch (PS), ${\alpha}-corn$ starch (CS), Na alginate (AL), dextrin (DEX), and carboxymethyl cellulose (CMC) were determined for olive flounder. The olive flounder averaging $150{\pm}8.0g$ were held in 300-L tanks at a density of 30 fish per tank. Chromic oxide was used as the inert marker. Feces were collected from the flounder by a fecal collector attached to a fish rearing tank. Apparent dry matter and energy digestibilities of flounder fed WF, PS, CS, and DEX diets were significantly higher than those of fish fed AL and CMC diets. Apparent crude protein digestibility coefficients of flounder fed PS and CS diets were significantly higher than those of fish fed AL, DEX, and CMC diets. Apparent crude lipid and nitrogen-free extract digestibility coefficients of flounder fed PS and DEX diets were significantly higher than those of fish fed WF, CS, AL, and CMC diets. The present findings indicate that PS and DEX could be effectively used as dietary carbohydrate energy compared to WF, CS, AL, and CMC for olive flounder.