• Annals of Clinical Neurophysiology
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• 제20권1호
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• pp.3-11
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• 2018

Skin biopsy and staining the specimens with immuno-reactive markers has been proven to be a useful method to demonstrate the pathologic status of small nerve fibers. Quantification of intraepidermal nerve fiber density using anti-protein gene product 9.5 antibody is a standard method to diagnose small fiber neuropathy. Skin biopsy also makes it possible to differentiate the nerve fibers according to their function by using different markers. Quantification of dermal structures with different types of nerve fibers could reveal the pathophysiologic mechanism of the disease state.

• Bulletin of the Korean Chemical Society
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• 제10권6호
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• pp.543-546
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• 1989

The photocycloaddition reaction of khellin with several olefins has been investigated. The photocycloadducts are formed regioselectively on furanyl 4',5'-double bond of khellin probably due to the localization of excitation energy on the furanyl 4',5'-double bond in the reactive excited state. The photocycloaddition reaction proceeds through the excited triplet state and the same product was formed when khellin is irradiated with dimethylfumarate or dimethylmaleate indicating the formation of common intermediate. The quantum yields of photocycloadduct formation, fluorescence, and intersystem crossing are very sensitive to proton-donating ability of solvents.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2019년도 춘계종합학술대회
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• pp.431-432
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• 2019

현재 전 세계 인구 76억명 중 스마트폰 사용자는 70%이상으로 스마트폰이 대중화가 되어 집이든 밖이든 누구나 자유롭게 이용하고 있다. 이에 본 논문에서는 HTML5와 CSS3를 기반으로 4차 산업혁명 대표 기술 중 하나인 IoT기술로 만든 제품을 주문받고 설치해주는 반응형 서비스판매 웹을 구성하고자 한다. 기존 웹과의 차별성과 고객 개개인의 만족도를 위해 제품판매 뿐만 아니라 주문제작 가능도 구현하여 고객의 욕구를 충족시키고자 한다.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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• pp.200.3-200.3
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• 2003

Reactive oxygen species(ROS) are produced at a high rate continuously as a by-product of aerobic metabolism. Several lines of evidence provided that ROS appears to cause to develop aging and various diseases. High level of hyaluronic acid with decreased molecular weight has been detected in patients with inflammatory diseases including rheumatoid arthritis. Hyaluronidase is an endohexosaminidase that initiates the degradation of hyaluronic acid with high molecular weight. Prunus persica Batsch var. davidiana Maximowicz has been known as a korean folk medicine for treatment of neuritis and rheumatism. (omitted)

• 멤브레인
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• 제2권1호
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• pp.49-58
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• 1992

Functionalized organic polymers have been used as supports for heterogenized homogeneous catalytic process[1]. Sprcific advantages of using these resins as support reagents have been reviewed[2-4]. These include: -ease of by-product separation from the main reaction product usuallyby simple filtration. -prevention of intermolecular reaction of reactive species or functional groups by simulating high dilution conditions[5]. -utility of the "fish-hook" principle in which a minor component in fished out of a large excess substrate by the insoluble polymer[6]. -the possibility of reusing recovered reagents as well as eliminating the use of volatile or noxious substances[7]. Catalysis by ion-exchange membranes is perhaps one of the latest examples of the use of a polymer-supported species. Conceptually, catalysts on membrane supports offer several possible advantages over traditional powder type systems. They are: (1) Membranes immobilize the catalyst, preventing agglomeration. (2) Filtration is unnecessary for the catalyst separation and so complete catalyst recovery is facilitated. (3) Catalytyic and separation processes can be combined, allowing membrane supported catalysts for the continous flow reactors. reactors.

• Preventive Nutrition and Food Science
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• 제8권2호
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• pp.124-129
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• 2003

This study was designed to investigate whether a methanol extract of chlorella can suppress oxidative stress and nuclear factor kB (NFkB) activation in lipopolysaccharide (LPS)-stimulated RAW 264.7 murine macrophage cells. Treatment of RAW 264.7 cells with chlorella methanol extract (25, 50, and 100 $\mu$g/mL) significantly reduced LPS-stimulated nitric oxide production in a dose-dependent manner. Treatments with chlorella methanol extract at all concentrations also reduced thiobarbituric acid-reactive substances accumulation and enhanced glutathione level at 50 and 100 $\mu$g/mL levels. The specific DNA binding activities of NFkB on nuclear extracts in cells treated with 50 $\mu$g/mL and 100 $\mu$g/mL chlorella methanol extracts were significantly suppressed. These results suggest that chlorella methanol extract has mild antioxidative activity and the ability to suppress intracellular oxidative stress and NFkB activation.

• 식품보건융합연구
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• 제4권3호
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• pp.6-11
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• 2018

In the Republic of Belarus as well as in the world acute problem of protecting forests from diseases and pests. The damage caused by root rot is essential, therefore, the problem of forest protection is an urgent task. The biologics has the greatest prospects in according with traditional methods of struggle. Deep method of cultivation of a mushroom Phlebiopsis gigantea with use of nutrient mediums on the basis of ethanol stillage and its components (fugat) is researched. Feasibility of use stillage as raw materials in production of a biological product for the wood protection against root decay is shown. The effect of different additives (sawdust, fodder yeast) on the accumulation of reactive biological product - oidy has been studed It was determined that the deep cultivation using sawdust of the highest accumulation oidy (1.5 $10^6units/ml$). It was also found that the stillage is the best breeding ground for fungus biomass accumulation (7.9 9.8 g / l) versus fugat (6.0 6.6 g / l). On the basis of research work the technological scheme for production of a biological product were developed. Based on the conducted studies, a technological scheme was proposed for obtaining a biological preparation by deep cultivation of the fungus Phlebiopsis gigantea.

• 한국수산과학회지
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• 제53권3호
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• pp.297-307
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• 2020

This study investigated the cosmetic effects of enzymatic hydrolytes of an aquatic by-product, fish skin. The skins of olive flounder Paralichthys olivaceus (PO) and Alaska pollock Gadus chalcogrammus (AP) were hydrolyzed using pepsin, Alcalase, and Protemax. Three enzymatic hydrolytes were obtained and the inhibitory effects of these hydrolytes on the aging-related enzymes tyrosinase, elastase, and collagenase were determined. The results indicated that the pepsin hydrolytes of PO and PA had stronger activities than the other hydrolytes. PO and PA also significantly reduced the intracellular reactive oxygen species levels in and improved the viability of H₂O₂-treated Vero cells; decreased nitric oxide production by and increased the cell viability of lipopolysaccharide-treated RAW 264.7 cells; and reduced intracellular reactive oxygen species levels and improved the viability of ultraviolet B irradiated HaCaT cells and human dermal fibroblasts. Furthermore, PO and PA remarkably reduced the intra- and extracellular melanin contents of alpha-melanocyte-stimulating hormone-stimulated B16F10 cells. These results demonstrate that PO and PA have potential for use in the cosmetic industry.

• 생명과학회지
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• 제26권8호
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• pp.948-954
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• 2016

포도주 가공 중에 발생하는 포도주 부산물의 항산화 활성 검토를 위해 포도주 부산물을 유기용매로 추출 및 분획하여 총 플라보노이드 및 총 페놀 함량을 측정하고 포도주 부산물의 항산화 효과를 분석하였다. 포도주 부산물의 분획 및 추출물의 총 플라보노이드의 양을 측정한 결과 MeOH 추출물은 A+M 추출물보다 높은 함량의 총 플라보노이드를 함유하는 것으로 나타났고 분획물들 중에서는 85% aq. MeOH 분획물이 105.1±0.93 mg/g으로 가장 높았으며 총 페놀 함량은 MeOH 추출물과 n-BuOH 분획물에서 높은 함량을 나타내었다. DPPH를 통한 라디칼 소거활성능을 측정한 결과, 0.5 mg/ml 첨가농도에서 MeOH 추출물은 74%의 소거율을 나타내었으며 분획물들 중에서는 n-BuOH 분획물이 72%의 소거활성을 나타내었다. 또한 ABTS+를 통한 라디칼 소거활성능을 측정한 결과, 0.5 mg/ml 첨가농도에서 85% aq. MeOH 및 n-BuOH 분획물들은 각각 90% 및 92% 소거활성을 나타내어 동일한 BHT 농도에서 92%인 것과 비교해 보았을 경우 합성항산화제만큼 높은 항산화능을 나타내었다. 세포내 활성 산소종 소거 효과를 나타내는 ROS 실험을 통해 0.1 mg/ml 첨가농도에서 MeOH 추출물과 n-BuOH 분획물은 각각 64%와 60%의 소거 활성을 나타었다. 이상의 결과를 종합해 보면 포도 및 포도씨와 비교했을 때 포도주 부산물의 경우 총 플라보노이드 및 총 페놀 함유량은 다소 낮았지만 항산화 효과도 지속되는 것으로 여겨지므로 향후 포도주 부산물의 응용이 기대되어진다.

• Advances in Traditional Medicine
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• 제7권1호
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• pp.85-93
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• 2007

Free radicals are known to play important role in pathophysiology of hepatic disorders and antioxidants are employed along with other chemotherapeutic agents in treatment of such diseases. In search of natural antioxidant, successive extracts of Hypericum (H.) hookerianum (Family: Hypericaceae) were evaluated by in vitro and in vivo methods. Extracts of aerial parts of H. hookerianum were subjected for 1,1-diphenyl 2-picryl hydrazyl radical scavenging activity (DPPH assay), nitric oxide radicals scavenging assay and thiobarbituric acid reactive substances (TBARS) assay. Methanolic extract was found to be more active than other extracts in DPPH and in vitro TBARS assay with $IC_{50}$ at 5.82 ${\pm}$ 1.33 ${\mu}g/ml$ and 49.78 ${\pm}$ 3.79 ${\mu}g/ml$ respectively. While petroleum ether extract showed more potentials in scavenging the nitric oxide radicals with $IC_{50}$ 220.97 ${\pm}$ 2.69 ${\mu}g/ml$. The administration of $CCl_{4}$ to the control animals caused decrease in the level of catalase and superoxide dismutase, together with significant increase in the level of TBARS in liver and kidney. Reversal of these changes towards normal group was observed by administration of H. hookerianum methanolic extract at 50 and 100 mg/kg body weight, while other extracts were found to be less active.