• 한국콘텐츠학회논문지
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• 제8권1호
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• pp.203-211
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• 2008

프로슈머의 등장과 동영상 콘텐츠가 디지털 방식으로 제작되면서 기존의 동영상을 다양한 방법으로 편집하는 UCC가 활성화되었다. 수많은 UCC가 제작되면서 저작권 문제, 복사에 따른 중복성 문제 그리고 저작물의 내용 문제 등이 대두되었다. 또한 기존의 동영상을 재구성할 경우 동영상 제작과정을 체계적으로 관리하고 동영상 콘텐츠를 표준화하여 재구성 및 재사용성에 따른 생산성을 높일 필요가 있다. 이에 본 연구에서는 UCC에 적합한 동영상 콘텐츠의 검색, 재사용성 그리고 저작권 등의 중요성을 인식하고, 동영상 콘텐츠의 제작 유통 재편집을 고려하여 e-Learning에 널리 채택되고 있는 SCORM 표준안을 기반으로 동영상 재사용 시스템을 구축하였다. 콘텐츠 객체의 메타데이터를 이용하여 동영상 콘텐츠의 검색과 재사용을 용이하게 할 뿐만 아니라 제작자 및 일반 사용자들이 자신의 취향에 따라 콘텐츠 객체들을 선택하고 재구성하여 자신만의 창의적 동영상 UCC를 쉽게 제작할 수 있다. Scene 단위의 동영상 객체별로 저작권을 관리하여 저작권 문제를 해소하는데 효과가 있을 것으로 본다. 인터랙티브 스토리텔링 기법의 하나로 SCORM의 시퀀싱 기능을 사용하여 사용자의 기호에 따라 맞춤형으로 동영상을 감상할 수 있는 개별화된 동영상 콘텐츠의 제작도 가능하다.

• Journal of Microbiology and Biotechnology
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• 제21권10호
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• pp.1001-1011
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• 2011

To explore bacterial diversity for elucidating genetic variability in acylhomoserine lactone (AHL) lactonase structure, we screened 800 bacterial strains. It revealed the presence of a quorum quenching (QQ) AHL-lactonase gene (aiiA) in 42 strains. These 42 strains were identified using rrs (16S rDNA) sequencing as Bacillus strains, predominantly B. cereus. An in silico restriction endonuclease (RE) digestion of 22 AHL lactonase gene (aiiA) sequences (from NCBI database) belonging to 9 different genera, along with 42 aiiA gene sequences from different Bacillus spp. (isolated here) with 14 type II REs, revealed distinct patterns of fragments (nucleotide length and order) with four REs; AluI, DpnII, RsaI, and Tru9I. Our study reflects on the biodiversity of aiiA among Bacillus species. Bacillus sp. strain MBG11 with polymorphism (115Alanine > Valine) may confer increased stability to AHL lactonase, and can be a potential candidate for heterologous expression and mass production. Microbes with ability to produce AHL-lactonases degrade quorum sensing signals such as AHL by opening of the lactone ring. The naturally occurring diversity of QQ molecules provides opportunities to use them for preventing bacterial infections, spoilage of food, and bioremediation.

• Mycobiology
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• 제42권2호
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• pp.206-209
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• 2014

In this study, we identified the causative agent of stem-end rot in potatoes that were grown in Gangwon alpine areas of Korea in 2013. The disease symptoms included appearance of slightly sunken circular lesion with corky rot on the potato surface at the stem-end portion. The fungal species isolated from the infected potatoes were grown on potato dextrose agar and produced white aerial mycelia with dark violet pigments. The conidiophores were branched and monophialidic. The microconidia had ellipsoidal to cylindrical shapes and ranged from $2.6{\sim}11.4{\times}1.9{\sim}3.5{\mu}m$ in size. The macroconidia ranged from $12.7{\sim}24.7{\times}2.7{\sim}3.6{\mu}m$ in size and had slightly curved or fusiform shape with 2 to 5 septate. Chlamydospores ranged from $6.1{\sim}8.1{\times}5.7{\sim}8.3{\mu}m$ in size and were present singly or in pairs. The causal agent of potato stem-end rot was identified as Fusarium oxysporum by morphological characterization and by sequencing the internal transcribed spacer (ITS1 and ITS4) regions of rRNA. Artificial inoculation of the pathogen resulted in development of disease symptoms and the re-isolated pathogen showed characteristics of F. oxysporum. To the best of our knowledge, this is the first study to report that potato stem-end rot is caused by F. oxysporum in Korea.

• 생명과학회지
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• 제14권5호
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• pp.840-846
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• 2004

본 연구는 한국산 겨우살이 lectin유전자 (A 및 B chain) 을 효모 (Saccharmyces cerevisiae) 에 형질 전환시키는 시스템을 사용한 것으로, 효모내 효과적인 lectin유전자 발현을 위하여 유전자 상에 Kozak translation initiation sequence를 PCR을 이용 삽입, 변형시켜 재 클로닝 하였다. 변형된 lectin A 및 B 유전자를 포함하는 재조합 플라스미드는 S. cerevisiae INVSc (MATa, his3 $\Delta$l, leu2, trpl-289, ura3-52) 에 형질전환 되었다. 형질전환된 효모는 ABI 3700 system을 이용한 DNA 염기서열 분석을 통해 확인되었고 재조합 한국산겨우살이 lectin 발현을 위해 2% galactose를 사용하여 유도발현되었다. 재조합 lectin A 및 B 단백질은 SDS-PACE 및 western blotting 분석을 수행한 결과 약 29kDa 크기로 확인되었다. 재조합 lectin은 세포내 가용성 단백질 1mg중 1.24∼l.75 $\mu\textrm{g}$ 수준으로 발현되어짐을 ELISA 분석을 통해 확인하였다. 한편 lectin 유전자는 galartose 유도발현 후 36시간이 되 었을 때 발현량이 최대가 되었으며 lectin A 유전자의 경우 48 시간 이후에는 발현이 억제되었다.

• 생명과학회지
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• 제17권8호통권88호
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• pp.1034-1038
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• 2007

하이퍼리신(Hypericin, HyH)은 예로부터 성 요한의 풀(St. John's Wort)로 널리 알려져 있는 서양고추나물(Hypericum perforatum)에서 추출되는 약리성분이다. 서양고추나물은 국내에서는 자생하지 않으나 같은 속의 고추나물(H. erectum) 등이 이속에 속하며 우리나라에 자생한다 . 고추나물을 조직배양으로 RNA를 추출하고 cDNA를 합성한 후 Hyp 유전자를 추출하여 서열화한 결과, 전체 크기는 732 bp로 나타났으며 서양고추나물의 HyH-1과 거의 99.8% 서열 일치를 나타내었다. 이 서열의 152개 아미노산 역시 서양고추나물의 항산화효소와 98% 일치하였으며 자작나무와 능금속 식물에서 유발되는 알레르기 유발유전자와 약 60% 일치를 나타내었다. 본 연구 결과 우울증치료제로 사용되는 하이퍼리신 추출에 우리나라 자생종인 고추나물이 이용될 수 있을 것으로 사료된다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.151-151
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• 2017

In Asia, rice production has some difficulties with reduction of farm household population and increase of elderly population. As a result, it has resulted in inefficiency and we needs to reduce labor force and improve labor productivity. Direct-seeding in rice could reduce labor and production costs, the area of direct seeding is increasing in japonica rice production in Asia. In direct seedling cultivation competition against weeds is one of most important concern. So, low temperature germinability and mesocotyl elongation should be considered. In this study, we evaluated the mesocotyl length and low temperature germination conducted association analysis using 137 korea core collections. An average length of mesocotyl among 137 core collections was skewed range from 0mm to 43mm. we searched candidate gene around target SNP. Such related traits, genome-wide association study (GWAS) analysis was carried out using GAPIT. Also, average mesocotyl length of 394 korea landrace cultivars was measured ranging from minimum 0 mm to maximum 34mm. 30 out of 394 Korea landrace cultivar conducted re-sequencing, and haplotype analysis of candidate gene. we searched these related resources, which including germination of low temperature and mesocotyl elongation. This could be used for the development of direct-seeding cultivars. The valiated accession of core collection and landrace cultivars will be used development of direct-seedling cultivar in the future.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.313-313
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• 2017

Cold stress is one of the most influenced factors to rice yield. In order to identify genes related to cold stress in fertility stage, genome-wide association study (GWAS) was conducted. Cultivated 129 rice germplasm were moved in the growth chamber under the condition of $12^{\circ}C/RH70%$(12h day/12h night when the rice plant was grown in 10 DBH(days before heading). Also, rice plant as control was moved in the green house under condition of $28^{\circ}C/RH70%$(12h day/12h night). After 4 days the plants were moved in a greenhouse. The fertility of rice plant were monitored after the grain were fully grown. The most tolerant rice germplasm to cold stress were Cheongdo-Hwayang-12 and IR38 as 63.1 and 61.8 of fertility and the most recessive rice germplasm were Danyang38 and 8 rice germplasm as 0. As a result of GWAS with re-sequencing data and fertility after cold treatment germplasm using genome association and prediction integrated tool (GAPIT), 99 single-nucleotide polymorphisms (SNPs) were observed by applying a significance threshold of -logP>4.5 determined by QQ plot. With SNPs region, 14 candidate genes responded to cold stress in fertility stage were identified.

• Asian-Australasian Journal of Animal Sciences
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• 제25권11호
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• pp.1521-1528
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• 2012

Previously identified QTL regions on BTA1 and BTA5 were investigated to validate the QTL regions and to identify candidate genes for growth and carcass traits in commercial cattle populations from the USA and Korea. Initially, a total of 8 polymorphic microsatellite (MS) markers in the BTA1 and 5 QTL regions were used for Chi-square tests to compare the frequencies of individual alleles between high and low phenotypic groups for the US (Michigan Cattleman's Association/Michigan State University; MCA/MSU) cattle. For a subsequent study, 24 candidate genes containing missense mutations and located within the QTL regions based on bovine genome sequence data were analyzed for genotyping in the two commercial cattle populations. Re-sequencing analyses confirmed 18 public missense SNPs and identified 9 new SNPs. Seventeen of these SNPs were used for genotyping of the MCA/MSU cattle (n = 98) and Korean native cattle (n = 323). On BTA1, UPK1B, HRG, and MAGEF1 polymorphisms residing between BM1312 and BMS4048 were significantly associated with growth and carcass traits in one or both of the MCA/MSU and Korean populations. On BTA5, ABCD2, IL22 and SNRPF polymorphisms residing between BL4 and BR2936 were associated with marbling and backfat traits in one or both of the MCA/MSU and Korean cattle populations. These results suggested that BTA 1 and 5 QTL regions may be segregating in both Korean Hanwoo and USA commercial cattle populations and DNA markers tested in this study may contribute to the identification of positional candidate genes for marker-assisted selection programs.

• Genomics & Informatics
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• 제13권4호
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• pp.137-145
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• 2015

Selective sweep can cause genetic differentiation across populations, which allows for the identification of possible causative regions/genes underlying important traits. The pig has experienced a long history of allele frequency changes through artificial selection in the domestication process. We obtained an average of 329,482,871 sequence reads for 24 pigs from three pig breeds: Yorkshire (n = 5), Landrace (n = 13), and Duroc (n = 6). An average read depth of 11.7 was obtained using whole-genome resequencing on an Illumina HiSeq2000 platform. In this study, cross-population extended haplotype homozygosity and cross-population composite likelihood ratio tests were implemented to detect genes experiencing positive selection for the genome-wide resequencing data generated from three commercial pig breeds. In our results, 26, 7, and 14 genes from Yorkshire, Landrace, and Duroc, respectively were detected by two kinds of statistical tests. Significant evidence for positive selection was identified on genes ST6GALNAC2 and EPHX1 in Yorkshire, PARK2 in Landrace, and BMP6, SLA-DQA1, and PRKG1 in Duroc. These genes are reportedly relevant to lactation, reproduction, meat quality, and growth traits. To understand how these single nucleotide polymorphisms (SNPs) related positive selection affect protein function, we analyzed the effect of non-synonymous SNPs. Three SNPs (rs324509622, rs80931851, and rs80937718) in the SLA-DQA1 gene were significant in the enrichment tests, indicating strong evidence for positive selection in Duroc. Our analyses identified genes under positive selection for lactation, reproduction, and meat-quality and growth traits in Yorkshire, Landrace, and Duroc, respectively.

• 한국균학회지
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• 제43권4호
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• pp.224-230
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• 2015

본 연구는 1992년부터 2015년까지 강원도 치악산 등 23개 지역에서 수집하여 수레바퀴애주름버섯(Mycena stylobates)으로 명명되어 농촌진흥청 국립농업과학원 식물균류 표본보존센터에 보존되어 있는 38개의 표본에 대하여 형태관찰과 자실체로부터 포자분리한 4개 균주의 ITS 염기서열분석을 수행하였다. 그 결과 새로운 종(Mycena yangsupensiae)으로 확인되어 종특징을 기술하고 미세구조를 도해하여 한국의 버섯 종목록에 등재하고자 한다.