• Ocean and Polar Research
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• v.43 no.4
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• pp.245-253
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• 2021

Surirella atomus Hustedt 1955, surirelloid diatom, were obtained from Hampyeong Bay, Mu-an, in the west coast of Korea. We describe the first record of S. atomus in Korea and report its ultrastructure and molecular characteristics for the first time. For molecular analysis, rbcL gene and cob gene were sequenced. This species is very small in size, making it difficult to observe its morphological features under a light microscope. Even with a scanning electron microscope, it has no unique structure in the valve. It is morphologically very simple. This rare species was known as a marine benthic species. However, it appears to be widely distributed in marine, freshwater, and brackish environments according to the literature documenting this species. In terms of phylogeny, S. atomus has been tentatively linked to Petrodictyon gemma, but the phylogenetic placement of S. atomus seems to be still uncertain, in a manner that is similar to S. febigeri. However, morphological observations derived from this study suggest that this species belongs to the genus Surirella.

• Journal of Trauma and Injury
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• v.24 no.2
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• pp.75-81
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• 2011

Purpose: The aim of this study was to analyze the influence of a trauma team's management. Methods: A total of 181 patients with severe trauma were retrospectively divided into two groups. Of these 181 patients, 81 patients without a trauma team admitted between April and October 2008 were assigned to Group 1, and 100 patients with a Trauma team admitted between April and October 2009 were assigned to Group II. We compared general characteristics, the length of stay in the emergency department (ED) and treatment outcomes (24-h packed RBC transfusion, length of intensive care unit (ICU) stay, length of hospital stay, in-hospital mortality, 24-h mortality) between these two groups. Results: The length of stay in the ED was significantly reduced in Group II compared to Group I ($p$=0.025). No significant differences were found in mean arterial pressure, Glasgow Coma Scale, Revised Trauma Score, Injury Severity Score, in-hospital mortality and 24-h mortality between the two groups. However, Group II had a lower amount of 24-h packed RBC transfusion and a shorter length of ICU and hospital stay than Group I, although these differences were not statistically significant. Conclusion: Through the establishment of a trauma team, the length of stay in the ED can be reduced remarkably. Furthermore, the need for 24-h packed RBC transfusions and the length of stay in the ICU and hospital were found to be decreased in patients managed by a trauma team.

• Journal of Life Science
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• v.18 no.8
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• pp.1115-1122
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• 2008

The purpose of this study is to identify the effect of rope-jumping to music with weight loading on middle school girls' blood lipid and growth hormone. Toward this end, the study targeted 24 middle school girls at Y Girls' Middle School in Yangsan, Gyeongnam, who have no health problems or exercise experience, after sufficiently explaining to them the study's purpose. The selected students were categorized into a music rope-jumping group (8), a music rope-jumping group with weight loading (8), and a control group (8). In a comparison of blood lipid within the exercise groups showed that the music rope-jumping groups' measures changed significantly by demonstrating increases or decreases in RBC, T-C, TG, HDL-C and LDL-C (p<0.05). The music rope-jumping group with weight loading showed significant differences in WBC, RBC, glucose, T-C, TG, HDL-C and LDL-C by demonstrating increases of decreases (p<0.05). A comparison between the exercise groups revealed that the music rope-jumping group showed significant increases in growth hormone (p<0.05). The music rope-jumping group with weight loading also showed significant increases in growth hormone (p<0.05). The study's results identified music jump-roping with weight loading to effect students' blood lipids and growth hormone.

• Journal of the Korean Society of Marine Environment & Safety
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• v.24 no.7
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• pp.967-972
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• 2018

The genus Chattonella belonging to the class raphidophyceae, is a harmful algal bloom species. Recently, its occurrence has been increasing and expanding along the Korean coast. Species identification of the genus Chattonella only by morphological observation is difficult due to the lack of rigid cell walls. In this study, the morphological characteristics and genetic affinity of Chattonella sp. isolated from Deungnyang Bay in 2017 were examined. We carried out single-cell isolation from field samples then sequenced three different areas using the single-cell PCR method: 1) parts of ribosomal operon, the large subunit (LSU) of the rDNA, 2) the chloroplast-encoded subunit psaA of Photosystem I, and 3) rbcL encoding the large subunit of the Rubisco gene. The cells were morphologically very similar to the general genus Chattonella ($74.0{\pm}10.1{\mu}m$ in length, $33.1{\pm}3.6{\mu}m$ in width). The three partial gene sequences were insufficient to justify distinction at the species rank. However, they clustered at 99-100 % sequence similarity with C. marina, C. marina var. antiqua and C. marina var. ovata.

• Journal of Plant Biotechnology
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• v.3 no.1
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• pp.39-44
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• 2001

Plastid transformation was attempted with soybean [Glycine max (L.) Merr.] leaves and photoautotrophic and embryogenic cultures by particle bombardment using the transforming vector pZVII that carries the coding sequences for both subunits of Chlamydomonas reinhardtii Rubisco and a spectinomycin resistance gene (aadA). Spectinomycin resistant calli were selected from the bombarded leaves but the transgene was not present, indicating that the resistance was due to mutations. The Chlamydomonas rbcL and rbcS genes were shown to be site-specifically integrated into the plastid genome of the embryogenic cells with a very low transformation efficiency. None of the transformed embryogenic lines survived the plant regeneration process so no whole plants were recovered. This result does indicate that it should be possible to insert genes into the plastid genome of the important crop soybean if the overall methods are improved.

• ALGAE
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• v.26 no.1
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• pp.33-40
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• 2011

Porphyroglossum is the last one of nine genera within the family Gelidiaceae that has yet to be analyzed by molecular markers. We analyzed rbcL and cox1 genes from P. zollingeri specimens collected near the type locality in Indonesia and compared them with other gelidioid algae. Thalli are cartilaginous, complanate, and up to 15 cm high. Abundant rhizoidal filaments are concentrated in the medullary layer. Tetrasporangial sori are on small, determinate ramuli. In all gene analyses, P. zollingeri consistently nested within Gelidium. The sister relationship of P. zollingeri to G. floridanum was well resolved. Because Gelidium has priority over Porphyroglossum, a new combination is proposed, viz. Gelidium zollingeri. Network analysis of the four cox1 haplotypes revealed many missing haplotypes, indicating high genetic diversity in the species.

• Fisheries and Aquatic Sciences
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• v.19 no.9
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• pp.38.1-38.5
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• 2016

We found specimens of foliose Bangiales from the subtidal zone of Udo, Jeju Island, Korea. In molecular analyses of rbcL sequences, these Korean specimens were almost identical to Miuraea migitae from Osaka, Japan. In the morphological comparison, Korean specimens were consistent with habitat, color, and vegetative characteristics with the description of M. migitae. This is the first record of M. migitae outside the type locality and Nagasaki in Japan. This study confirms that new or unrecorded species of the order Bangiales may be discovered from subtidal habitats.

• Weed & Turfgrass Science
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• v.4 no.1
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• pp.26-34
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• 2015

Korean Poaceae includes approximately 80 species of the agricultural weeds. Precise species identification is the first step for more effective weed management in the agricultural fields. However, the identification of species in Poaceae is not easy without the assistance of taxonomists or identification experts although they are relatively easy to distinguish from the plants of the other family by the unique characteristics of caryopsis. Thus, DNA barcode was suggested as an alternative powerful technique for species identification by using short sections of DNA from a specific region of the genome. Two standard barcode markers of vascular plants, chloroplast rbcL and matK, and a supplementary nuclear ribosomal Internal Transcribed Spacer (ITS) region were used for barcode of major Korean Poaceae weeds, 403 individuals of 84 taxa. All the barcode markers revealed a good level of sequencing success with the lowest 73.7% for matK and the highest 88.8% for rbcL. The barcode sequences were deposited to the National Center for Biotechnology Information (NCBI) database for public use. Combined matK and ITS showed very high resolving power with 92.9%. Besides the identification of weeds for weed managment, the generated DNA barcode data could be used for many other applications such as rapid biodiversity assessment and conservation prioritization.

• Journal of the Korean Magnetics Society
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• v.27 no.3
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• pp.92-97
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• 2017

It is widely known that pulsed magnetic field (PMF) is very useful tool to manipulate chemical and physiological processes in human body. The purpose of our study is to observe dynamics of rouleaux patterns of red blood cells (RBC) under PMF. The aggregation of RBCs or rouleaux formation is caused by fibrinogen in blood plasma. The maximum magnetic field intensity is 0.27 T and pulse time of 0.102 msec and pulse repetition rate was 1 Hz. PMF stimulus was applied to the palm of left hand for 5, 10, 15 and 20 min. Live blood analysis was used in vitro in order to quantitatively estimate the velocity of RBC exposed to PMF stimulus. The velocity of stacked-RBC of 10 minute PMF stimulus was increased up to $8{\times}10^{-4}m/sec$, but it decreased rapidly as the time passed. The results of present study have adduced that PMF stimulus on hand provide the improvement of RBC rouleaux formation, increase of RBC's moving velocity as well as low blood viscosity.

• Proceedings of the Zoological Society Korea Conference
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• 2001.10a
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• pp.118.3-119
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• 2001

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