• Reproductive and Developmental Biology
• /
• v.30 no.3
• /
• pp.207-212
• /
• 2006

The aim of this study was to examine the effect of mechanical enucleation methods, aspiration and squeezing, on the developmental ability of nuclear transfer bovine embryos. Enucleated oocytes made by both enucleation methods were fused to adult ear skin cells. After 7 days of culture, developmental ability up to blastocyst stage was similar in both squeezing($33.6{\pm}15.7%$) and aspiration enucleation methods($31.9{\pm}13.4%$). The proportion of blastocysts at Day 8 of culture was also similar between the aspiration($37.8{\pm}10.4%$) and squeezing enucleatign s($35.3{\pm}15.1%$). The mean cell number in Day 7 blastocysts was also similar between the both groups(aspiration: $110.3{\pm}39.2$ vs. squeezing: $103.7{\pm}42.8$). The ratio of apoptotic cells was also found to be not significant different between the both groups(aspiration: $2.8{\pm}2.6%$ vs. squeezing: $4.3{\pm}4.4%$). These results suggest that aspiration and squeezing methods, as mechanical enucleation technique, are both useful for the production of bovine somatic cell nuclear transfer embryos.

• Journal of dental hygiene science
• /
• v.11 no.3
• /
• pp.189-197
• /
• 2011

Objectives : The systematic health education to form the basic healthy lifestyle should be realized from elementary school, so oral health education at elementary school can determine the whole lifetime oral health. The elementary school health teacher's recognition and behavior who in charge of health promotion of students is important. Therefore, the study was conducted to enhance oral health education. Methods : Total 114 people among of 131 from health teacher Gwangju elementary school. Survey system is configured by referring to PRECEDE model. Results : In behavioral diagnosis the proportion of oral Health Education is less than 10%(58.8%), mostly educated in activity time (86.0%), the health teachers educate when it is needed(53.5%). In predisposing diagnosis in the eight areas of health education, the oral health education is ranked as fourth, fifth. Even in the next year project, the oral health education ratio was 21.9 percent. In enabling diagnosing every year the Oral health education training experienced rate is 13.2%, satisfaction rate is 33.3%. In reinforcing diagnosing disability element in the regular education course are the lack of oral health-related information (46.7%), lack of materials needed for education (30.6%), lack of training opportunities (21.4%). Conclusion : In further research, oral health education textbooks, materials and methods should be developed. At the foundation of there developments, the elementary oral health education program should be more fully developed and conducted and also the evaluation of its effectiveness will need.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.42 no.1
• /
• pp.8-16
• /
• 2013

We investigated the effect of red cabbage extract (RCE) on cell death in MDA-MB-231 human breast cancer cells. Cells were cultured in the presence 1.0, 1.5, and 2.0 mg/mL concentrations of RCE for 24 hours. MTT assays demonstrated that mitochondrial dehydrogenase activities decreased in a dose-dependent manner in cells (p<0.05). In contrast, the proportion of dual staining with Hoechst 33342/ethidium bromide (EtBr) for cell death increased in a dose-dependent manner in cells (p<0.05). Flow cytometry assays revealed that cell death caused by an apoptotic program increased in a dose-dependent (p<0.05). Also, increased ROS accumulation in cells, as revealed by DCF-DA staining, was observed in a dose-dependent fashion (p<0.05). The apoptosis suppressor gene Bcl-2 decreased significantly at the mRNA level. Pro-apoptotic genes Bax and caspase-3, genes that are related to the last stage of apoptosis significantly increased. The Bcl-2/Bax ratio which is an important indicator of apoptosis, was found to have significantly decreased dose dependence. These results taken together indicate that the effect of red cabbage extract induces cell death in MDA-MB-231 human breast cancer cells.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.12
• /
• pp.258-264
• /
• 2018

In this study, the improved performance of sprayed green soil was evaluated by incorporating functional additives. The optimal mixing ratio of the thickener and super-absorbent polymer, as an additive for moisture supply to the growth of plants within the range of mixing ratios that gives sufficient strength of green soil, was 5% and 1%, respectively. Using Portland cement as a main binder, the pH of the green soil was 9.1. To solve this alkali problem, the mixing proportion was improved so that the pH of the green soil was approximately 7.2 by mixing more than 10% of the chelate resin. The soil conductivity was measured to be 280 ~ 350mS/m under all the mixing conditions. This satisfied the criterion of less than 1000mS/m on the slope surface. As a result of measuring the soil hardness of the green soil prepared under the optimal mixing conditions of functional additives, it satisfied the criteria of 18 ~ 23mm when sprayed under a 1 bar pressure. The rebound rate was less than 15% when spraying green soil on a 75 % slope, and the hardness of the sprayed green soil was more than 18 mm.

• Journal of the Korean GEO-environmental Society
• /
• v.12 no.9
• /
• pp.71-78
• /
• 2011

Cement treating technique, such as deep mixing method, has been used widely to stabilize the dredged clayey soil for many years. Despite of its effectiveness in treating soil by cement, several efforts have also been made to try to reduce the side effect of the cement that used to stabilize the dredged clay. However, authors considered that more detailed study on the physical and mechanical properties of lean-mixed soil-cement has been required to establish the design procedure to apply the practical problems. Therefore, in this study, the curing time and mixing ratio was used as key parameters to estimate the physical and mechanical properties including long-term behavior. The unconfined strength of lean-mixed soil-cement increase continuously during curing period, 270 days, while increasing rate becomes low in ordinary cement-treated dredged clay. We also concluded that cement-treated dredging clay shows apparent quasi overconsolidation behavior even in low cement proportion. By this study, fundamental approach was carried out for effective reuse of very soft dredged clayey soil both in mechanical and environmental aspect. It can be also expected that this study can propose a basic design data to use the lean-mixed soil cement.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.27 no.6
• /
• pp.130-137
• /
• 2023

By using XRF (X-ray fluorescence), this study investigates the variation of chemical properties in cement pastes at elevated temperatures. High-temperature conditions were prepared by using an electric furnace, planning a total of 11 target temperatures ranging from room temperature to 1000 ℃. A standard library of geo-quant basic was applied for the analysis of 12 elements in cement paste, including Ca, Si, Al, Fe, S, Mg, Ti, Sr, P, Mn, Zn and K. The results revealed that, as the temperature increased, the proportion of each element in the cement paste also increased. With the exception of a few elements present in extremely low amounts in the cement pastes, the variation in the composition ratio of most elements exhibited a strong correlation with temperature, with an R-squared value exceeding 0.98. In this study, cement pastes exposed to normal and high-temperature environments were compared. The authors established that the reasons for the different results in this comparison can be explained from the same perspective as when comparing raw cement with cement paste. Furthermore, this study discussed the potentially most dominant parameter when investigating the properties of cement paste using XRF.

• 대한공업교육학회지
• /
• v.36 no.2
• /
• pp.219-238
• /
• 2011

The purpose of this study was to analyze the industrial education items in the teacher recruitment examination for secondary school and make a better proposal. To achieve the purpose, all the industrial education items, which had taken an examination for ten times from the school year 2002 to the most recent year 2011, were analyzed. The results of this study were as follows; First, the number and score ratio of industrial education items had been increased quarterly from the school year 2002 to 2011, but had same sharing in all subjects and fixed since the school year 2010. Second, as the industrial education items had been increased quarterly, they were taken from more various subcategory of industrial education. Recently, the industrial education items on "complex domain" including two more subcategory of industrial education were increased. Third, the industrial education items with science education items was compared from the school year 2009 to 2011. The items on PCK(Pedagogical Content Knowledge) had been increased in the industrial education but the proportion of PCK was lower than science education.

• Korean Journal of Clinical Pharmacy
• /
• v.30 no.2
• /
• pp.113-119
• /
• 2020

Background: Reducing the total anticholinergic burden (AB) in older adults is recommended owing to the several peripheral and central adverse effects. This study aimed to identify the AB status of patients admitted to geriatric centers for assessing the influence of the pharmacist-involved multidisciplinary geriatric team care on reducing the AB. Methods: We retrospectively reviewed the medical records of 328 older patients hospitalized in geriatric centers from July 1, 2018 to June 30, 2019, who received comprehensive geriatric assessment and pharmaceutical interventions from a multidisciplinary geriatric team. We measured the total AB scores for the medications at the time of admission and upon hospital discharge using the Korean Anticholinergic Burden Scale (KABS). The pre-admission factors associated with high AB (KABS score ≥3) at the time of admission were identified. Results: The proportion of patients with high AB significantly decreased from 41.8% (136/328) at the time of admission to 25.0% (82/328) on discharge (p<0.001). The pre-admission AB of patients transferred from skilled nursing facilities (odds ratio[OR]: 2.85, 95% CI: 1.26-3.75), taking more than 10 medications (OR: 3.70, 95% CI: 1.55-8.82), suffering from delirium (OR: 2.80, 95% CI: 1.04-7.50), or depression (OR: 2.78, 95% CI: 1.04-7.41) were significantly high. Antipsychotics were the most frequent classes of drugs that contributed to the total KABS score at the time of admission, followed by antihistamines. Conclusions: This study demonstrated that the multidisciplinary teams for geriatric care are effective at reducing AB in older adults. The factors associated with high AB should be considered when targeting pharmaceutical care in geriatric individuals.

• Journal of Science Education
• /
• v.45 no.2
• /
• pp.247-256
• /
• 2021

Visual representation has been a useful tool in mathematical problem solving because it vividly express and structure the variables in the problem. But its effects may vary according to the types of problems. So, this study analyzes the survey results on the 5^th graders' visual representations using questionnaire consisting of the routine problems and the non-routine problems. The results are follows: The rate of correct answers in routine problems was higher than that of the non-routine problems. Even though the subjects were asked to solve the problem using visual representations, the ratio of solving the problem using the numerical expression was high in the routine problems. On the other hand, the rate of solving the problem using visual representation was high in the non-routine problems. The number of respondents who used visual representation in the non-routine problems was twice as many as that of the routine problems. But, among the subjects who used visual representation in the non-routine problems, the proportion of incorrect answers was also high, which resulted in using visual pictures. So, it is necessary to provide an experience that can use various types of the visual representations for problem solving and pay attention to the process of converting problems into visual representations.

• The Journal of the Convergence on Culture Technology
• /
• v.8 no.2
• /
• pp.47-52
• /
• 2022

The purpose of this study is to identify the risk level of Chinese commercial banks' loan assets and to analyze what factors affect the stability of Chinese commercial banks. In addition, Chinese commercial banks are classified based on the asset size of 200 billion yuan, and the difference in stability according to size is investigated. The analysis results are as follows. First, it was estimated that as the proportion of household and corporate loans of commercial banks in China increased, the stability of banks decreased. Although the Chinese financial authorities are currently restricting the conservative management of loan assets, it will be necessary to preemptively manage risk on loan assets by setting an appropriate standard for loan-to-deposit ratio in the future. Second, as a result of analyzing the stability of large banks based on 200 billion yuan of bank assets, it was estimated that the stability of large banks was lower. As large banks are likely to conduct aggressive loan asset management, continuous management of non-performing assets is required in the future. This study will serve as a measure for improving the stability of commercial banks in China by estimating the effect of loan asset management of Chinese commercial banks on financial stability. In particular, by examining the stability of large banks, a strategy for sustainable development of the financial industry is required by diagnosing the weaknesses of large banks.