• Korean Journal of Food Science and Technology
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• v.40 no.2
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• pp.238-242
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• 2008

In this study, hydrogen sulfide ($H_2S$) production was examined during beer fermentation using two ale and two lager yeast strains. In the lager yeast fermentation, a large amount of $H_2S$ was produced in the early fermentation stages when the yeast were actively fermenting wort, indicating a positive relationship between the level of H2S production and the yeast growth rate during fermentation. The ale yeasts produced much lower levels of H2S than the lager yeasts. In the lager fermentation, a higher fermentation temperature shortened the fermentation period, but much higher levels of $H_2S$ were produced at higher temperatures. American pilsner lager yeast fermenting at $15^{\circ}C$ produced a relatively high level of $H_2S$ at the end of fermentation, which would require a longer aging time to remove this malodorous volatile sulfur compound. Not including the English ale strain, which produced a higher level of H2S at lower temperatures, the ale yeast produced lower levels of $H_2S$ at lower temperatures, suggesting that each strain has an optimum fermentation temperature for H2S production.

• Food Science and Preservation
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• v.30 no.1
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• pp.132-145
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• 2023

In this study, culture conditions were optimized to confirm the feasibility of Acetobacter pasteurianus as a starter for fermentation vinegar. Acetobacter pasteurianus strain can be used as a food ingredient. The optimal temperature and pH conditions of the selected Acetobacter pasteurianus SRCM101388 were 28℃ and pH 6.00, respectively. The response surface methodology (RSM) was used to optimize the composition of the medium, and Plackett-Burman design (PBD) was used to obtain the effective selection of culture medium, resulting in that glucose, sucrose, and yeast extract had the highest effect on increasing biomass. The optimal concentration, which was performed by central composite design (CCD), were determined to be 10.73 g/L of glucose, 3.98 g/L of sucrose, and 18.73 g/L of yeast extract, respectively. The optimal concentrations of trace elements for the production of biomass were found to be 1 g/L of ammonium sulfate, 0.5 g/L of magnesium sulfate, 2 g/L of sodium phosphate monobasic, 2 g/L of sodium phosphate dibasic, and the final optimized medium was pH 6.10. When incubated in a 5 L jar fermenter, the SRCM101388 strain showed a faster-dissolved oxygen (DO) reduction at a lower agitation rate (rpm), and it was able to grow even at reduced DO level when aeration was maintained. The amount of final biomass produced was 2.53±0.12×10⁹ CFU/mL (9.40±0.02 log CFU/mL) when incubated for 18 hours at 150 rpm, 0.5 vvm, pH 6.0, and 28℃.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.41-44
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• 2008

Studies were made to investigate the difference of the shiitake productivity according to the use of sawdust and liquid spawn on middle-temperature type strain. Treatments inoculated with sawdust spawn have shown that yields were dispersed according to flushing periods and fruit-bodies occurred sporadically on medium. However, treatments inoculated with liquid spawn have shown that yield was relatively concentrated at 1st flushing period and fruit-bodies were densely occurred on medium. At total yield and the number of fresh fruit-bodies until seventh flushing, treatments inoculated with liquid spawn were more than those inoculated with sawdust spawn. Also, biological efficiency (B.E.) of inoculation with 20 ml liquid spawn which produced 30% of the medium weight was highest as 69%, but biological efficiency of inoculation with 10 g sawdust spawn was lowest as 42%. And, although rate of fruit-bodies over 10 g in total yield was high on treatments inoculated with sawdust spawn, amounts of fruit-bodies over 10 g were not different among treatments.

• Korean Journal of Microbiology
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• v.46 no.2
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• pp.127-133
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• 2010

The purpose of this work was to investigate the antibacterial activity derived from a lactic acid bacterium, Lactococcus lactis HK-9, isolated from the feces of a 2-day newborn infant. We characterized the physiological and biochemical properties of this strain. Both the BIOLOG system and phylogenetic analysis using 16S rRNA sequencing were utilized for identification, and the strain was assigned to the Lactococcus lactis species, designated as L. lactis HK-9, and registered in GenBank as [GU936712]. We monitored growth rate, production of lactic acid and acetic acid as metabolites, and pH during growth. The maximum concentrations of lactic acid and acetic acid reached 495.6 mM and 104.3 mM, respectively, and the initial pH of the cultures decreased from 7.0 to 4.1 after incubating for 60 h. HPLC was used to confirm the production of lactic acid and acetic acid. Significant antibacterial activity of the concentrated supernatant was demonstrated against Gram-positive (e.g., Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes, MRSA) and Gram-negative (e.g., Escherichia coli, Salmonella enteritidis, Pseudomonas aeruginosa, Klebsiella pneumoniae, Shigella sonnei) bacteria by the plate diffusion method. The antibacterial activity was sensitive to protease, and the molecular weight of the presumed bacteriocin molecule was estimated to be about 4 kDa by tricine-SDS-PAGE.

• Parasites, Hosts and Diseases
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• v.23 no.2
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• pp.293-299
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• 1985

This study is to verify the protective ability against experimental Naegleria meningoencephalitis by immunization with Naegleria fowleri in mice. Naegleria fewleri, strain 0359, and Naegleria gruberi, strain EGB, were used in this study, and cultured in CGVS medium akenically. Inbred BALB/C mice, weighing about 20g, were immunized by three intraperitoneal injection of $1{\times}10^6$ N. fowleri trophozoites at the interval of one week. This N. fowleri trophozoites antigen was fixed with 5% formaldehyde. N. fowleri trophozoites from culture were homogenized with soiicator at $4^{\circ}C$ as monitored by phase contrast microscopy, and their membrane and cell content preparations were made for the immunization of mice. Their inoculation dose in volume was equivalent to the $1{\times}10^6$ trophozoites in each injection for immunization. And N. gruberi trophosoites, whieh was fixed with 5% formaldehyde, were also used for immunisation. Mice were inoculated intranasally with $5{\times}10^4$ N. fowleri trophozoites in a 511 suspension under anesthesia by as intraperitoneal injection of about 1 mg secobarbiturate. Nervousness, rotation or sluggish behaviour were observed in the mice which were infected with N. fewleri. Necrotic lesion was demonstrated in the anterior portion of brain, especially in the olfactory lobe. The inflammatory cell infiltration with numerous H. fowleri trophozoites was noticed. This pathological changes were more extensive in the control than in the experimental groups. Mice were dead due to experimental primary amoebic meningoencephalitis that developed between 8 days and 23 days after inoculation. Mortality rate of the mice was low in the immunized experimental group. Mean survival time, which is the survival duration of mice from the infection to death, was prolonged significantly in the immunized mice except in the mice immunized with JV, fowleri membrane. Even in the mice immunized with N. gruberi, survival time was delayed. In summary, the effectiveness of immunization is demonstrated in terms of protective immunity against Naegleria meningoencephalitis in mice.

• Journal of the Korean Society of Marine Environment & Safety
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• v.26 no.4
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• pp.412-418
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• 2020

Generally, the propeller shaft that constitutes the ship shaft system has different patterns of behavior due to the ef ects of engine power, propeller load and eccentric thrust, which increases the risk of bearing failure by causing local load variations. To prevent this, different studies of the propulsion shaft system have been conducted focused the relative inclination angle and oil film retention between the shaft and the support bearing, mainly with respect to the Rules for the Classification of Steel Ships. However, in order to secure the stability of the propulsion shaft via a more detailed evaluation, it is necessary to consider dynamic conditions, including the transient state due to sudden change in the stern wakefield. In this context, a 50,000 DWT vessel was analyzed using the strain gauge method, and the effects of propeller shaft movement were analyzed on the starboard rudder turn which is a typical transient state during normal continuous rate(NCR) operation in ballast draught condition. Analysis results confirm that the changed propeller eccentric thrust acts as a force that temporarily pushes down the shaft to increase the local load of the stern tube bearing and negatively affects the stability of the shaft system.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.4
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• pp.261-265
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• 2000

To develope the enhanced bacterial strains capable of biodegradation for various chlorinated aromatic compounds, 100 bacterial strains were isolated from soil samples of suburbs of Taejon, Cheongju, and Jeonju by the enrichment culture. These strains can degrade pentachlorophenol (PCP) which is a kind of wood preservatives. Nineteen strains of the isolates were selected by fast colony-forming rate on solid minimal media containing PCP as an only source of carbon and energy. These strains were identified to genus level. Fifteen strains were identified as Pseudomonas, 1 strain as Acinetobacter and 3 strains were not. Genus Alcaligenes strains were not found among them. Pseudomonas sp. MU135. MU139, MU163 and MU 184 were able to degrade for 4 kinds of chlorinated compounds, PCP, 2,4-D, MCPA and 3CB. Pseudomonas sp. If was observed that MU139 exhibits the highest degradability in liquid minimal media at 72 hours after inoculation. Pseudomoans sp. MU147, MU177, MU184 and MU192 also degraded the compounds at higher rates. As the results, Pseudomonas sp. MU139 and unidentified strain MU184 had biodegrability for broad range of chlorinated compounds and higher rates of degradation for PCP.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.32 no.2A
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• pp.65-73
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• 2012

In this study, we tested structural performance of Half-Decked PSC girder which was developed for applying to long span bridge. We operated 4 point bending test with 60 m span full scale girder designed as simple bridge with hinge-roller boundary condition. Actuators were set on the both sides of girder, 5.5 m away from the center, and 4 stages of cyclic loading was applied at rate of 1 kN/sec. Through stages 1 to 4, loading and unloading 1,000 kN, 1,200 kN, 1,500 kN, and 2,000 kN were repeated and displacement, strain of concrete and steel, crack of girder were checked. From these results, the strength of girder was assessed and resilience and ductility were observed after removing the load. Since initial flexural crack occurred in the vicinity of 1,400 kN, non-linearity of load-displacement curve appeared and definite residual strain was measured at that point. The test result showed that initial cracking load was over twice the DB-24 load which means the developed girder had sufficient strength. To verify the experimental results, we numerically analyze the test and confirmed that the data were similar with results from the test above. Half-Decked PSC type of 60 m-girder developed in this study showed its adequate structural capacity through static loading test, which proved that possibility of applying the girder to actual bridges practically.

• Tunnel and Underground Space
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• v.14 no.1
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• pp.69-77
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• 2004

Investigation of the time-dependent behavior of rock and the associated mechanisms are of key interest in long-term stability analysis of many engineering applications. In this study, creep tests were performed on Daejeon granite samples of 25.4mm diameter under uniaxial compression at varying stress levels. The effect of moisture was investigated by testing both air-dried and fully water-saturated samples. The creep behavior of Daejeon granite exhibited three distinctive stages of primary, secondary and tertiary creep. The ultimate strength of granite under a constant stress decreased considerably with time. Saturation and immersion of the test specimen in water markedly increased the total creep strain as well as the secondary creep rate. The experimental creep curves are fitted to Burger's model as well as two other empirical models suggested by previous researchers. A number of the parameters determined for each model are dependent on stress and influenced by the presence of water. Based on the experimental results, an empirical relation between the applied stress and the time-dependent strain is established separately for each air-dried and fully water-saturated Daejeon granite.

• Korean Journal of Microbiology
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• v.36 no.2
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• pp.79-83
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• 2000

Activated sludge samples were collected from a municipal sewage treatment plant and used for enrichment of microbial consortia with aniline as the sole carbon and nitrogen source. Threc aniline-degrading bacteria were obtained lrom microbial consortia and an isolate which has excellent aniline degradability was selected for this study. The isolate was Gram-negative, and identified and designated as Delfha sp. JK-2 on the basis of various physiological and biochemical tests. 10 mM aniline was completely degraded within 24 hours after inoculation of the culture. Ammonium ion was liberated in the medium transiently during the incubation and disappeared when aniline was completely degraded. Addition of glucose as a supplementary source to aniline minimal media showed significant decrease in aniline degradat~on rate for the strain Effective degradation of aniline was achieved by the addition of 0.5% nitrate as a nitrogen source, and resulted in approximately 80% higher aniline degradation compared to the absence of nitrate. Phylogenetic analysis based on 16s [DNA sequence revealed that the strain was closely related to De@ia acidovorans, with 96% overall similarity. The 16s [DNA sequence of JK-2 was also found to be closely related to those of six other clonal types, including Acidovoru, Aquaspirillum. Xylophilus, Variovorm, and Rhodofernr.