• The Korean Journal of Physiology
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• v.8 no.1
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• pp.55-65
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• 1974

The effect of ginseng on the ATPase activity of rabbit ref cell membrane has been investigated. The experiments were also designed to determine whether the components of ginseng could be attributed to the effect on ATPase activity which dependent upon sodium plus potassium and is sensitive to ouabain. The following results were observed. 1. The activity of the $Na^{+}-K^{+}-ATPase$ from red cell membrane is stimulated by ginseng, and the concentration of ginseng for half-maximal activity is about 15 mg%. The pH optimum for the ginseng sensitive component is 7.6. 2. The portion of the enzyme activity stimulated by ginseng is completely abolished by ouabain. 3. The activating effect of ginseng on the ATPase, with a given concentration of sodium in the medium, is increased by raising the potassium concentration but activity ratio is decreased. 4. The activating effect of ginseng on the ATPase, with a given concentration of potassium in the medium, is increased by raising the sodium concentration but the activity ratio is decreased. 5. The ATPase activity is increased by small amounts of calcium but inhibited by larger amounts and the rate of activity by ginseng is constant. 6. The action of ginseng on the ATPase activity was not related to the sulfhydryl group of cysteine, the amino group of lysine, the imidazole group of histidine, the quanidinium group of arginine, the carboxyl group of aspartic acid, or the hydroxyl group of threonine. 7. The activating effect of ginseng on the ATPase activity may be not due to a saponin which is contained in ginseng.

• Journal of Advanced Navigation Technology
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• v.19 no.5
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• pp.403-410
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• 2015

ADS-B can provide high accurate position information and faster update rate than Radar system and it is a technique that can supplement or replace the Radar. Recently ADS-B has been applied to the actual aircraft operation because to increase air transportation traffic and required to high accurate surveillance. In this study, we surveyed analysis of position deviation distribution analysis and received actual ADS-B trajectory data for conformed precise surveillance on the near airport area using ADS-B message. For that, we received the precision instrument approach ADS-B trajectory data using instrument landing system, and can analyse about target deviation distribution on the final approach segment about precision instrument approach. The result of analysis is mean distance of target deviation -0.04 m and standard deviation 6.71 m on between ADS-B target and extended runway centerline. Also that is to conformed the ADS-B message trajectory available to provide relatively exact surveillance information.

• The Journal of the Korea Contents Association
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• v.9 no.11
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• pp.10-17
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• 2009

Wyner-Ziv video coding is a new video compression paradigm based on distributed source coding theory of Slepian-Wolf and Wyner-Ziv. Wyner-Ziv coding enables light-encoder/heavy-decoder structure by shifting complex modules including motion estimation/compensation task to the decoder. Instead of performing the complicated motion estimation process in the encoder, the Wyner-Ziv decoder performs the motion estimation for the generation of side information in order to make the predicted signal of the Wyner-Ziv frame. The efficiency of side information generation deeply affects the overall coding performance, since the bit-rates of the Wyner-Ziv coding is directly dependent on side information. In this paper, an improved side information generation method using field coding is proposed. In the proposed method, top fields are coded with the existing SI generation method and bottom fields are coded with new SI generation method using the information of the top fields. Simulation results show that the proposed method improves the quality of the side information and rate-distortion performance compared to the conventional method.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.8 no.5
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• pp.1010-1019
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• 2004

In this paper, we represent Nakagami-m fading channel as finite-State Markov Channel (FSMC) and analyze the performance of wireless communication system with varying the fading channel condition. In FSMC model, the received signal's SNR is divided into finite intervals and these intervals are formed into Markov chain states. Each state is modeled by a BSC and the transition probability is dependent upon the physical characterization of the channel. The steady state probability and average symbol error rate of each state and transition probability are derived by numerical analysis and FSMC model is formed with these values. We found that various fading channels can be represented with FSMC by changing state transition index. In fast fading environment in which state transition index is large, the channel can be viewed as i.i.d. channel and on the contrary, in slow fading channel where state transition index is small, the channel can be represented by simple FSMC model in which transitions occur between just adjacent states. And we applied the proposed FSMC model to analyze the coding gain of random error correcting code on various fading channels via computer simulation.

• Journal of Microbiology and Biotechnology
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• v.21 no.5
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• pp.483-493
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• 2011

An acid phosphatase (HppA) activated by $NH_4Cl$ was purified 192- and 34-fold from the periplasmic and membrane fractions of Helicobacter pylori, respectively. SDS-polyacrylamide gel electrophoresis revealed that HppA from the latter appears to be several kilodaltons larger in molecular mass than from the former by about 24 kDa. Under acidic conditions (pH${\leq}$4.5), the enzyme activity was entirely dependent on the presence of certain mono- and/or divalent metal cations (e.g., $K^+$,$ NH_4{^+}$, and/or $Ni^{2+}$). In particular, $Ni^{2+}$ appeared to lower the enzyme's $K_m$ for the substrates, without changing $V_{max}$. The purified enzyme showed differential specificity against nucleotide substrates with pH; for example, the enzyme hydrolyzed adenosine nucleotides more rapidly at pH 5.5 than at pH 6.0, and vice versa for CTP or TTP. Analyses of the enzyme's N-terminal sequence and of an $HppA^-$ H. pylori mutant revealed that the purified enzyme is identical to rHppA, a cloned H. pylori class C acid phosphatase, and shown to be the sole bacterial 5'-nucleotidase uniquely activated by $NH_4Cl$. In contrast to wild type, $HppA^-$ H. pylori cells grew more slowly. Strikingly, they imported $Mg^{2+}$ at a markedly lowered rate, but assimilated urea rapidly, with a subsequent increase in extracellular pH. Moreover, mutant cells were much more sensitive to extracellular potassium ions, as well as to metronidazole, omeprazole, or thiophenol, with considerably lowered MIC values, than wild-type cells. From these data, we suggest that the role of the acid phosphatase HppA in H. pylori may extend beyond 5'-nucleotidase function to include cation-flux as well as pH regulation on the cell envelope.

• Journal of Microbiology and Biotechnology
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• v.20 no.9
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• pp.1314-1321
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• 2010

Sulfur metabolism in S. cerevisiae is well established, but the mechanisms underlying the formation of sulfide remain obscure. Here, we investigated by real-time RT-PCR the dependence of expression levels of MET3, MET5/ECM17, MET10, MET16, and MET17 along with SSU1 on nitrogen availability in two wine yeast strains that produce divergent sulfide profiles. MET3 was the most highly expressed of the genes studied in strain PYCC4072, and SSU1 in strain UCD522. The strains behaved differently according to the sampling times, with UCD522 and PYCC4072 showing the highest expression levels at 120 h and 72 h, respectively. In the presence of 267 mg assimilable N/l, the genes were more highly expressed in strain UCD522 than in PYCC4072. MET5/ECM17 and MET17 were only weakly expressed in both strains under any condition tested. MET10 and SSU1 in both strains, but MET16 only in PYCC4072, were consistently upregulated when sulfide production was inhibited. This study illustrates that strain genotype could be important in determining enzyme activities and therefore the rate of sulfide liberation. This linkage, for some yeast strains, of sulfide production to expression levels of genes associated with sulfate assimilation and sulfur amino acid biosynthesis could be relevant for defining new strategies for the genetic improvement of wine yeasts.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.271-280
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• 2010

Vibrio alginolyticus, a Gram-negative marine bacterium, is one of the causative agents of fish vibriosis. Its virulence factors and pathogenesis mechanism are barely known, except for some extracellular products (ECPs) that are known to be regulated by quorum sensing system. Therefore, the present study used a microarray to analyze the transcription profiles of the wild-type V. alginolyticus and a deletion mutant of luxO, the pivotal regulator in Vibrio quorum sensing systems, which resulted in the identification of a putative virulence factor, MviN. Quantitative real-time reverse transcription PCR confirmed that the transcription of mviN was upregulated in the luxO mutant when compared with wild-type, and down regulated in a luxO-con complemented strain. Furthermore, Western blotting indicated that MviN was greatly induced during the late-exponential and stationary phases of growth, indicating that the expression of MviN was cell-density dependent and quorum sensing regulated in V. alginolyticus. Meanwhile, the mviN null mutant displayed a much slower growth rate than the wild type, signifying the essential role of MviN in V. alginolyticus. Western blotting also revealed that MviN was present as an extracellular protein in V. alginolyticus. When epithelioma papulosum cyprini (EPC) cells were treated with the ECPs of the mviN mutant, no cytotoxicity was observed, whereas EPC cells treated with the wild type exhibited pathological changes, which increased with the ECPs concentration and treatment time. Therefore, the results demonstrated that MviN is a LuxO-regulated ECPs component and involved in the pathogenicity of V. alginolyticus.

• Journal of Korean Society of Environmental Engineers
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• v.22 no.12
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• pp.2227-2238
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• 2000

The interfacial features of suspension system made of $CaCO_3$ particles have been investigated for the purpose of designing its effective treatment process. For the examination of variation of electrokinetic potential as a function of pH. the value of potential was observed to shift in the negative direction, which was thought to be due to the adsorption of hydroxide ion on the particle surface. Adsorption of surfactant on suspended particles resulted in the change of surface charge and shift in electrokinetic potential, which was dependent upon the sign of head charge and concentration of surfactant. Addition of inorganic salts affected stability of suspension greatly and sedimentation rate of suspension was influenced by the electric valence and amount of ions produced by dissolution of inorganic coagulants. DLVO theory made it possible to construct a energy profile diagram and a close correlation was found between experimental result and theoretically derived consequences. Non-specific adsorption of indifferent electrolyte resulted in the compression of electrical double layer and specific adsorption induced the shift of IEP and PZC in the opposite direction.

• Journal of Advanced Navigation Technology
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• v.16 no.4
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• pp.611-618
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• 2012

ICAO has recommended the introduction of New CNS/ATM based on GNSS technologies and Data link communication to purpose increasing air traffic demand. The CNS/ATM is composed of communication, Navigation, Surveillance and Air Traffic Management. Cockpit Display Traffic Information(CDTI) that is equipment to share air traffic information to Pilot, Traffic Controller and ground vehicles has been built based on Automatic Dependent Surveillance-Broadcast(ADS-B) information in Surveillance field. This paper is research that what is the effect between pilot and controller through CDTI. Based on ATC communication between Pilot and Controller, We construct simulation environment and did a comparative analysis of it. according to result, We can verify that CDTI makes aircraft Call sign, Position, Altitude information read rate, and notice of air traffic information between Pilot and Controller more improve than ATC environment and Existing Radar System.

• Journal of Nutrition and Health
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• v.39 no.8
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• pp.742-747
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• 2006

This study was performed to investigate the lipolytic effects of Portulaca oleracea L. extract in 3T3-L1 adipocytes. The Portulaca oleracea L. was extracted with extrusion method using twin-screw extruder under $58{\sim}60rpm$ screw speed, $4{\sim}5kg/hr$ feed rate, $140^{\circ}C$ extrusion temperature. The lipolytic action of Portulaca oleracea L. extract was estimated by measuring the amount of glycerol and free fatty acids (FFA) released from 3T3-L1 adipocytes and by measuring the cellular lipid content in 3T3-L1 adipocytes. The hormone sensitive lipase (HSL) mRNA level was analyzed using quantitative real-time PCR. The Portulaca oleracea L. extract at 1 to $100{\mu}g/ml$ suppressed lipid accumulation. The release of glycerol and FFA into the medium, and the mRNA level of HSL were significantly increased by the addition of Portulaca oleracea L. extract at dose-dependent manner. In conclusion, the Portulaca oleracea L. extract was suggested to have the lipolytic effect through release of lipolytic products (FFA and glycerol) of triacylglyceride to the culture medium and suppression of lipid accumulation via up-regulation of HSL gene expression in 3T3-L1 adipocytes.