• Korean Journal of Veterinary Research
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• v.48 no.3
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• pp.243-250
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• 2008

A calmodulin-dihydrofolate reductase (DHFR) sandwich fusion protein was generated by insertion of calmodulin into the $\beta$-bulge region of DHFR to observe the effects of structurally constraining the calmodulin structure. The calcium binding properties of the sandwich protein were almost identical to calmodulin. Similar to calmodulin ($10.7 {\mu}M$), the sandwich protein bound four equivalents of calcium, with half saturation ($K_{0.5}$) observed at a [$Ca^{2+}$] of $8{\mu}M$. However, nicotinamide adenine dinucleotide (NAD) kinase activation property of the sandwich protein was lower than that of calmodulin. The sandwich protein activated NAD kinase, but to only half of the level obtained with calmodulin. The K 0.5 for both calmodulin and the sandwich protein were approximately the same (1-2 nM). Methylation analyses of the sandwich protein show that insertion of calmodulin into DHFR results in a large decrease in methylation. The $V_{max}$ observed with the sandwich protein (95 nmole/min/ml) was only 22% of the value observed with calmodulin (436 nmol/min/ml) in the presence of calcium. Addition of trimethoprim to the reaction significantly inhibited the observed methylation rate. Overall, the data suggest that the insertion of calmodulin into the DHFR structure has little effect on calcium binding by the individual lobes of calmodulin, but may constrain the lobes in a manner that results in altered interaction with the calmodulin-dependent proteins, and severely perturbed the methyltransferase recognition site.

• Journal of the Korean Vacuum Society
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• v.15 no.5
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• pp.465-474
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• 2006

A gas fueling system composed of a gas reservoir, an on-off valve, and a gas transferring tube, which is the simplest construction for the pre-programmed gas puffing, was simulated by numerically solving the time-dependent one-dimensional gas flow equation. The purpose of the simulation is to establish the relationship between the gas flow pattern (the elapsed time to the maximum flow, the maximum flow rate, the gas pulse duration) and the system parameters (the filling pressure and the volume of the gas reservoir, and the length and the diameter of the gas transferring tube).

• Journal of the Korean Vacuum Society
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• v.11 no.4
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• pp.249-255
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• 2002

For a formation of membrane structures, single crystal silicon wafers have been anisotropically etched with solutions of KOH and KOH-IPA. The etching rate was observed to be strongly dependent upon the etchant temperature and concentration. Mask patterns for the etching experiment was aligned to incline $45^{\circ}$on the primary flat of the silicon wafer. The different etching characteristics were observed according to pattern directions and etchant concentration. When the KOH concentration was fixed to 20 wt%, the U-groove etching shape was observed for the etching temperature of above $80^{\circ}C$, and V-groove shapes observed at below $80^{\circ}C$. Hillocks, which were generated at the etched silicon surfaces, has been decreased as the increasing of the etchant temperature and concentration.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2004.05a
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• pp.395-399
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• 2004

Four microalloyed steels containing B were investigated in terms of hardenability, mechanical properties and microstructure depending upon the cooling rates in order to develop the steel grade for the cold forged fasners. The alloy with the largest DI value among 4 alloys, which contains $0.12\%\;C,\;1.54\%\;Mn,\;0.65\%\;Cr,\;0.11\%V,\;0.040\%Ti\;and\;0.0033\%B$, showed the larest shift to the right hand side in the TTT diagram, implying the wide allowable cooling rate range subsequent to hot rolling in long bar processing, Mechanical tests indicated that yield strength are dependent upon the DI value in water quenched specimens but other properties showed almost the same values. In the same grade of steel, the increase in cooling rates causes the decrease in elongation but the increase in strength, reduction of area and Charpy impact values. Microstructural examination in steel grade with the larest DI values revealed martensitic structure In the water quenched state, a mixture of martensite and bainite in the oil quenched, and ferrite + pearlite in the air cooled and the forced air cooled but the latter showed finer microstructure.

• Korean Journal of Optics and Photonics
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• v.13 no.3
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• pp.240-244
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• 2002

We proposed a novel temperature sensor based on the phenomena that the phase difference between principal polarization states in an optical retarder is a function of temperature. The polarization state of an optical beam is changed as it passes through the optical retarder, with the change dependent on the temperature. The temperature of the optical retarder is determined by comparison of the power difference between principal polarization states. A temperature sensor was successfully implemented using a polarization maintaining fiber of length 100 mm as the optical retarder. The change rate of phase difference on temperature was 0.236$^{\circ}C$$_{-1}$ and the measurement error was $\pm$0.038$^{\circ}C$ over the temperature region of -2.6$^{\circ}C$~3.4$^{\circ}C$.

• YAKHAK HOEJI
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• v.38 no.2
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• pp.114-122
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• 1994

A study on the absorption mechanism of cefixime(CF), an oral ${\alpha}-amino$ group deficient cephalosporin antibiotic, has been undertaken through the rat jejunum and nasal cavity using an in situ simultaneous perfusion technique developed in our laboratory. CF was well absorbed in the jejunum and nasal cavity of rats at pH 5.0, but not at pH 7.0. CF absorption was studied over four orders of magnitude in concentration to determine saturability. Disappearance of CF in the perfusate followed first-order kinetics at all tested concentrations. The apparent first-order absorption rate constant was found to be dependent on the concentration over the range of $0.1\;mM{\sim}3\;mM$ in the jejunum and nasal cavity of rats. Inhibitors were added to determine the competitive inhibition of CF absorption. The presence of L-tyrosine, L-phenylalanine, alanine-alanine, glycine-glycine and cefadroxil produced the significant inhibition of CF absorption in the nasal cavity and jejunum. However, there was no evidence of the inhibition in the presence of cefazolin. In addition, The CF absorption in the nasal cavity and jejunum was inhibited significantly by ouabain and 2,4-dinitrophenol(DNP). This study suggested that CF is absorbed across the rat nasal cavity and jejunum by carrier-mediated transport mechanism and energy consuming system.

• Membrane Journal
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• v.6 no.2
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• pp.109-116
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• 1996

This work is a fundamental study for applying hybrid process coupling adsorption with microfiltration to waste-water treatment. Phenol was separated by adsorption on powdered activated carbon, adsorbed phenol with activated carbon was separated by microfiltration. As the particle size in suspension increased, filtration resistance decreased, and effect of particle concentration on resistance was less pronounced. The rate of uptake was greatly dependent on the degree of phenol loading. For a smaller amounts of activated carbon, the change of permeate concentration before break point and phenol loading with time were steeper than in the case of large amounts. Permeate concentration before break point decreased with decreasing particle size, this could be due to the increase of outer surface of particle and film mass transfer coefficient.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.39 no.2
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• pp.43-54
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• 2013

In an attempt to regain function and aesthetics in the craniofacial region, different biomaterials, including titanium, hydroxyapatite, biodegradable polymers and composites, have been widely used as a result of the loss of craniofacial bone. Although these materials presented favorable success rates, osseointegration and antibacterial properties are often hard to achieve. Although bone-implant interactions are highly dependent on the implant's surface characteristics, infections following traumatic craniofacial injuries are common. As such, poor osseointegration and infections are two of the many causes of implant failure. Further, as increasingly complex dental repairs are attempted, the likelihood of infection in these implants has also been on the rise. For these reasons, the treatment of craniofacial bone defects and dental repairs for long-term success remains a challenge. Various approaches to reduce the rate of infection and improve osseointegration have been investigated. Furthermore, recent and planned tissue engineering developments are aimed at improving the implants' physical and biological properties by improving their surfaces in order to develop craniofacial bone substitutes that will restore, maintain and improve tissue function. In this review, the commonly used biomaterials for craniofacial bone restoration and dental repair, as well as surface modification techniques, antibacterial surfaces and coatings are discussed.

• The Journal of Korean Association of Computer Education
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• v.3 no.2
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• pp.115-127
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• 2000

Korean error detectors of word processors have defects such as inconvenience that users choose one of error groups, lower detecting rate of 60%, and slow processing time. In this study, I proposed a resolution method of these defects. For these, I applied bidirectional longest match strategy for morphological analysis to improve processing time. I suggested dictionaries and several algorithms such as seperation of compound noun and assistant declinable words, correction of typing error to improve processing time and to guarantee correction accuracy. I also suggested a distinguishable method for dependent noun/suffix and Josa/Eomi where many ambiguities are generated, and a distinguishable method for Korean "로써/로서" to improve the reliability of the correction system.

• Structural Engineering and Mechanics
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• v.23 no.6
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• pp.651-674
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• 2006

The fatigue cracks initiate and propagate in the Armored Vehicle Launch Bridge (AVLB) components, especially like the splice doubler angle, splice plate, and bottom chord, due to the cyclic loading by repeated AVLB-launchings and tank-crossings. In this study, laboratory fatigue tests were conducted on six aluminum 2014-T6, four aluminum 7050-T76511, and four ASTM A36 steel compact-tension specimens to evaluate the crack growth behavior of the materials used for the components. The experimental results provide the relationship (Paris Law) between crack growth rate, da/dn, and stress intensity range, ${\Delta}K$, whose material dependent constants C and m can later be used in the life estimation of the components. Finite Element Method (FEM) was used to obtain the stress intensity factor, K, of the components with cracks. Because of the complexity of loading conditions and component geometry, several assumptions and simplifications are made in the FEM modeling. The FEM results, along with the results obtained from laboratory fatigue tests, are then utilized to estimate critical crack length and remaining life of the components.