• 제목/요약/키워드: rat

검색결과 10,313건 처리시간 0.035초

Development of an Immunosensor to Detect Rat IgG Using Impedance Analyser

  • No D. H.;Kang S.;Kim G. Y.;Chung S. H.;Park Y. H.;Om A. S.;Cho S. I.
    • Agricultural and Biosystems Engineering
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    • 제5권1호
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    • pp.21-24
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    • 2004
  • Antibody based biosensors are very selective and ultra-sensitive. Antigen-antibody reactions have been used in immunoassays. In this research, a biosensor which uses antigen-antibody reaction was developed to measure and detect rat IgG. Because the antigen-antibody reaction is a physical bounding between antigen and antibody, there are several ways to measure an antigen-antibody reaction. Among the methods, impedance analysis has short measuring time and possibilities of analyzing various properties of the reaction using frequency analysis. Rat IgG could be detected with developed biosensor and impedance analyzer. The biosensor showed good repeatability and availability of detecting concentration changes of rat IgG.

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Fluoride가 적출장기(摘出臟器)의 Catecholamine 유리(遊離) 및 Monoamine Oxidase 활성도(活性度)에 미치는 영향(影響) (The Influence of Sodium Fluoride on the Release of Catecholamine from Perfused Organs and Monoamine Oxidase Activity)

  • 천연숙;김성숙;이경희;신경철
    • 대한약리학회지
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    • 제8권2호
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    • pp.41-47
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    • 1972
  • Fluorides were supposed to exert a stimulatory action on the catecholamine release. In this study, the authors attempted to investigate the action of sodium fluoride on the catecholamine release from the isolated perfused cow adrenal gland and rat heart. And also the inhibitory effect of sodium fluoride on the monoamine oxidase activity in rat heart and liver mitochondria was investigated. The monoamine oxidase activity was measured by the conversion of benzylamine to benzaldehyde. The results obtained were follows; 1. Sodium fluoride stimulated the release of catecholamine from the isolated perfused cow adrenal gland and rat heart. 2. Sodium fluoride inhibited the rat heart and liver mitochondrial monoamine oxidase activity.

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Modulation of Branched-Chain Amino Acid Metaolism by Exercise in Rats

  • Kim, Hyun-Sook
    • Journal of Nutrition and Health
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    • 제27권9호
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    • pp.892-900
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    • 1994
  • A variety of important roles for branched-chain amino acids in metabolic regulation has been suggested. Branched-chain $\alpha$-keto acid dehydrogenase(BCKAD) complex is a rate limiting enzyme in branched-chain amino acid metabolism. The purpose of this study was to examine the effects of exercise on the activity and activity state of branched-chain $\alpha$-keto acid dehydrogenase in rat hert and liver thssues. Forty-eight Sprague-Dawley rats were assigned into three experimental groups : sedentary control, exercised, or exercised-rested. Submaximal exercise(running) for two hours significantly increased basal activity without a change in total activity in both tissues, with a concomitiant increase in activity state of the enzyme complex. At 10 min post-exercise, heart enzyme activity significantly decreased, though not to the control level, while liver enzyme activity remained unchanged. These data suggested that the exercise-induced increase in branched-chain $\alpha$-keto acid decarboxylation in rat tissues may not be the result of enzyme synthesis, but rather is due to increased activity of the BCKAD.

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래트(Rat)의 운동을 위한 트레이드밀의 개발에 관한 연구 (A Study on the development of treadmill for the exercise of rat)

  • 김주명;최은영
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2001년도 기술교육위원회 창립총회 및 학술대회 의료기기전시회
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    • pp.76-81
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    • 2001
  • This paper place the focus the development of treadmill for rat in order to verify the effectiveness of exercise therapy. Up to now, in many medical laboratories treadmill have had to be remodeled for experiments with animal. Thus, it is necessary to develop the treadmills for animals only including rats. The treadmill has a DC motor which can make 600 revolutions per minute(600 RPM) and tension of the belt of the treadmill can be adjusted to prevent shift of the belt to any side. Velocity can be selected from 10m to 30m per minute increasing by 1m. The motor is controled by pulse width modulation(PWM) control mode using a 8bit microprocessor. In the future, the roller and motor need to be combined for improvement of stability and decrease of noise.

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출생전후 및 성별로 본 쥐의 간세포에서의 G-6-PD 활성 (Sex Differences in the Glucose-6-Phosphate Dehydrogenase Activity of the Rat Livers at Various Stages of Development)

  • Hahn, Sahsook
    • 한국동물학회지
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    • 제17권1호
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    • pp.17-22
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    • 1974
  • Pentose phosphate shunt의 첫 작용때 나타나는 효소 glucose-6-phosphate dehydorgenase의 활성을 출생전 및 출생후 연령에 따라서 쥐(white rat)의 간세포를 암수 성별로 spectrophotometer를 써서 측정하였다. 이 활성이 16일 된 배에서 가장 높았고 (54 I.U.), 그때부터 출생후 13일 까지 조금씩 저하되지만 (17 I.U.), 다시 서서히 증가해서 19일에는 53 I.U.까지 높아졌다. 성별로 본 결과는 일반적으로 수컷보다 암컷에서 G-6-PD의 활성이 약간 높게 나타났다.

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Determination of Branched-Chain α-Keto Acid Dehydrogenase Activity in Rat Tissues

  • Kim, Hyun-Sook;Johnson, Wayne A.
    • BMB Reports
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    • 제28권1호
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    • pp.12-16
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    • 1995
  • The branched-chain ${\alpha}$-keto acid dehydrogenase (BCKAD) complex is a rate limiting enzyme which catalyzes the oxidative decarboxylation of branched-chain ${\alpha}$-keto acids. Numerous studies have suggested that BCKAD is subject to covalent modification in vitro via phosphorylation and dephosphorylation, which are catalyzed by a specific kinase and phosphatase, respectively. The biggest difficulty in the assay of BCKAD activity is to arrest the interconversion between the active and inactive forms. BCKAD activity was determined from fresh rat heart and liver tissues using homogenizing and assay buffers containing inhibitors of phosphatase and kinase. The results suggest that a radiochemical assay using ${\alpha}$-keto[1-$^{14}C$]-isovalerate as a substrate for the enzyme can be applied as a reliable method to determine in vitro enzyme activity with arrested interconversion between the active and inactive forms of the BCKAD complex.

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산앵도(Vaccinium koreanum) 잎 유래 화합물들의 Aldose Reductase 및 혈전응집에 미치는 효과 (Effects of Vaccinium koreanum on Rat Lens Aldose Reductase and Platelet Aggregation)

  • 주화균;정상훈;조선행;신국현;임순성
    • 생약학회지
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    • 제34권4호통권135호
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    • pp.314-317
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    • 2003
  • Inhibitory effects of the whole extract and isolated compounds from the leaves of Vaccinium koreanum (Ericaceae) on rat lens aldose reductase (RLAR) and on platelet aggregation were investigated for the prevention or the treatment of chronic diabetic complications. Arbutin, isolated from n-butanol fraction of methanol extract, exhibited a potent inhibitory effects on rat platelet aggregation induced by ADP $(IC_{50}=0.12\;{\mu}M)$ and collagen $(IC_{50}=0.039\;{\mu}M)$ and showed same inhibitory activities with positive control, tetramethylene glutaric acid, on RLAR.

송화분이 흰쥐의 간장에 미치는 영향(II) (Effect of Pine Pollen on Rat Liver(II))

  • 한준표
    • 동아시아식생활학회지
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    • 제10권3호
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    • pp.200-207
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    • 2000
  • This study was conducted to investigate the nutritional constituents of pine pollen and the effects of pine pollen on rat liver. Using the chemical components analysis method, the constituents of pine pollen are crude protein(13.97%). crude lipid (6.50%), crude ash (3.25%). Detectable in pine pollen are main fatty acids and non-essential amino acids such as glutamic acid and aspartic acid such as oleic and linoleic acid and 18kinds of amino acid including essential amino acids. The effects of pine pollen on rat liver fed with a high fat diet for 6 weeks showed significant decrease in total lipid and triglyceride as compared with the group fed with a fat diet only. Total cholesterol content decreased by 20.33%. No significant difference was found in total protein content of each group. Pine pollen Is efficacious in preventing geriatric diseases (arteriosclerosis, hyper-lipidemia and cardiovascular system-related diseases).

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In Vivo Evaluation of Multi Lamellar Vesicle Liposome’s Percutaneous Absorption and Stability

  • Joung, Min-Seok;Park, Jong-Oan;Seo, Bong-Seok;Ryu, Chang-Duck
    • 대한화장품학회지
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    • 제27권1호
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    • pp.29-42
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    • 2001
  • We had prepared MLV liposome with Hibiscus Esculentus Ext.(HEE) which have fluorescent light in order to evaluate its percutaneous absorption about hairless rat skin. Then we investigated particle size of MLV using confocal laser scanning microscope(CLSM) and transmission electron microscope(TEM), respectively. Stability of MLV liposome and penetration of MLV liposome to hairless rat skin was measured by CLSM. As a result of experiments, MLV was globular shape and the rage of particle size was 0.3-0.5$\mu\textrm{m}$ mostly. Cream-type MLV had high stability comparatively. When we treated with MLV to rat skin, skin penetration was enhanced, especially, the optimum concentration of MLV on penetration to rat skin was 10%. Optimum penetration time was 6hr-12hr. And MLV-type HEE was more effective on percutaneous absorption than HEE-cream or liposome-type HEE.

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흰쥐 간조직에서의 비소처리 영향 및 비소 전처리 효과 (Effect of Arsenic Treatment and Pretreatment in Rat Liver Tissue)

  • 노미경;손성향;부문종;김옥용
    • Applied Microscopy
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    • 제24권4호
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    • pp.78-85
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    • 1994
  • Sodium arsenite ($NaAsO_2$) was injected to the rat subcutaneously for the study of the acute toxicity of arsenite on hepatocytes, and the effects of pretreatment of arsenite and glutathione on the lethalty of the arsenite treated rats. Arsenite treated rat hepatocytes showed vacuolated cytosol and shrinked nuclear and expanded perinuclear space and cytoplasmic membrane whirl. Rats pretreated with BSO (L-Buthionine-SR-Sulfoximine), less survived than arsenite treated alone. It means that glutathione acts as a protecting agent against the arsenite. Subcutaneous sublethal dose (10mg/kg body weight) treatment was showed the protecting activity to lethality of lethal dose (15mg/kg body weight) treated rat. 10mg/kg body weight sublethal dose effects appeared in six hours intervals of between treatments.

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