Agricultural and Biosystems Engineering

Korean Society for Agricultural Machinery (한국농업기계학회)
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Development of an Immunosensor to Detect Rat IgG Using Impedance Analyser

  • No D. H. (National Institute of Agricultural Engineering, Rural Development Administration, KOREA) ;
  • Kang S. (National Institute of Agricultural Engineering, Rural Development Administration, KOREA) ;
  • Kim G. Y. (National Institute of Agricultural Engineering, Rural Development Administration, KOREA) ;
  • Chung S. H. (National Institute of Agricultural Engineering, Rural Development Administration, KOREA) ;
  • Park Y. H. (Dept. of Animal Science, College of Life & Environmental Sciences, Korea University) ;
  • Om A. S. (Dept. of Food & Nutrition, College of Human Ecology, Hanyang University) ;
  • Cho S. I. (Dept. of Biosystem Engineering, College of Agriculture and Life Sciences, Seoul National University)
  • Published : 2004.06.01
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Abstract

Antibody based biosensors are very selective and ultra-sensitive. Antigen-antibody reactions have been used in immunoassays. In this research, a biosensor which uses antigen-antibody reaction was developed to measure and detect rat IgG. Because the antigen-antibody reaction is a physical bounding between antigen and antibody, there are several ways to measure an antigen-antibody reaction. Among the methods, impedance analysis has short measuring time and possibilities of analyzing various properties of the reaction using frequency analysis. Rat IgG could be detected with developed biosensor and impedance analyzer. The biosensor showed good repeatability and availability of detecting concentration changes of rat IgG.

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