• 제목/요약/키워드: rapid preparation

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Comparison of Upgraded Methods for Detecting Pathogenic Escherichia coli in Foods Using Centrifugation or Filtration

  • Choi, Yukyung;Lee, Heeyoung;Lee, Soomin;Kim, Sejeong;Lee, Jeeyeon;Ha, Jimyeong;Oh, Hyemin;Yoon, Yohan
    • Food Science of Animal Resources
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    • v.37 no.6
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    • pp.799-803
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    • 2017
  • In the present study, centrifugation and filtration pretreatments were evaluated to decrease sample preparation time and to improve the sensitivity and specificity of multiplex polymerase chain reaction (PCR) for the detection of low levels of pathogenic Escherichia coli in various foods. Pathogenic E. coli (E. coli NCCP11142, E. coli NCCP14037, E. coli NCCP 14038, E. coli NCCP14039, and E. coli NCCP15661) was inoculated into pork, beef, and baby leafy vegetables at 1, 2, and 3 Log CFU/g. The samples were shaken 30 times (control), then centrifuged or filtered. DNA extracts from the samples were subjected to PCR using the $Powerchek^{TM}$ Diarrheal E. coli 8-plex Detection Kit. In the pork samples, no E. coli was detected in the control samples, while E. coli were detected in 100% of 3-Log CFU/g inoculated and centrifuged samples, and in 100% of 2 and 3-Log CFU/g inoculated, and filtered samples. In the beef samples, all control samples appeared to be E. coli-negative, while E. coli was detected in 50-75% of centrifuged samples, regardless of inoculated level, and in 100% of 2 and 3-Log CFU/g inoculated, and filtered samples. In baby leafy vegetables, E. coli were not detected in 25-50% of the control samples, while E. coli were detected in 0-25% of the centrifuged samples, and 75-100% of the filtered samples, depending on the inoculum amount. In conclusion, filtration pretreatment can be used to minimize sample preparation time, and improve the sensitivity and specificity of rapid detection of pathogenic E. coli in various foods.

A Simple, Rapid, and Automatic Centrifugal Microfluidic System for Influenza A H1N1 Viral RNA Purification

  • Park, Byung Hyun;Jung, Jae Hwan;Oh, Seung Jun;Seo, Tae Seok
    • Proceedings of the Korean Vacuum Society Conference
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    • 2013.08a
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    • pp.277.1-277.1
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    • 2013
  • Molecular diagnostics consists of three processes, which are a sample pretreatment, a nucleic acid amplification, and an amplicon detection. Among three components, sample pretreatment is an important process in that it can increase the limit of detection by purifying nucleic acid in biological sample from contaminants that may interfere with the downstream genetic analysis such as nucleic acid amplification and detection. To achieve point-of-care virus detection system, the sample pretreatment process needs to be simple, rapid, and automatic. However, the commercial RNA extraction kits such as Rneasy (Qiagen) or MagnaPure (Roche) kit are highly labor-intensive and time-consuming due to numerous manual steps, and so it is not adequate for the on-site sample preparation. Herein, we have developed a rotary microfluidic system to extract and purify the RNA without necessity of external mechanical syringe pumps to allow flow control using microfluidic technology. We designed three reservoirs for sample, washing buffer, and elution buffer which were connected with different dimensional microfluidic channels. By controlling RPM, we could dispense a RNA sample solution, a washing buffer, and an elution buffer successively, so that the RNA was captured in the sol-gel solid phase, purified, and eluted in the downstream. Such a novel rotary sample preparation system eliminates some complicated hardwares and human intervention providing the opportunity to construct a fully integrated genetic analysis microsystem.

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Rapid Identification of Staphylococcus Species Isolated from Food Samples by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

  • Kim, Eiseul;Kim, Hyun-Joong;Yang, Seung-Min;Kim, Chang-Gyeom;Choo, Dong-Won;Kim, Hae-Yeong
    • Journal of Microbiology and Biotechnology
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    • v.29 no.4
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    • pp.548-557
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    • 2019
  • Staphylococcus species have a ubiquitous habitat in a wide range of foods, thus the ability to identify staphylococci at the species level is critical in the food industry. In this study, we performed rapid identification of Staphylococcus species using Matrix-Assisted Laser Desorption/Ionization Time-of-Flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS was evaluated for the identification of Staphylococcus reference strains (n = 19) and isolates (n = 96) from various foods with consideration for the impact of sample preparation methods and incubation period. Additionally, the spectra of isolated Staphylococcus strains were analyzed using principal component analysis (PCA) and a main spectra profile (MSP)-based dendrogram. MALDI-TOF MS accurately identified Staphylococcus reference strains and isolated strains: the highest performance was by the EX method (83.3~89.5% accuracy) at species level identification (EDT, 70.3~78.9% accuracy; DT, less than 46.3~63.2% accuracy) of 24-h cultured colonies. Identification results at the genus level were 100% accurate at EDT, EX sample preparation and 24-h incubation time. On the other hand, the DT method showed relatively low identification accuracy in all extraction methods and incubation times. The analyzed spectra and MSP-based dendrogram showed that the isolated Staphylococcus strains were characterized at the species level. The performance analysis of MALDI-TOF MS shows the method has the potential ability to discriminate between Staphylococcus species from foods in Korea. This study provides valuable information that MALDI-TOF MS can be applied to monitor microbial populations and pathogenic bacteria in the food industry thereby contributing to food safety.

Effects of the Occupation and Activity Nature of the Middle-Aged on Retirement Preparation (중년층의 직업특성과 활동특성이 은퇴준비에 미치는 영향)

  • Shin, Gye-Soo;Cho, Sung-Sook
    • The Journal of the Korea Contents Association
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    • v.11 no.11
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    • pp.296-312
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    • 2011
  • Korea has been suffering from financial hardship with low birth rate and rapid growing of advanced aged people because low economic growth and low interest rate have continued after IMF foreign-exchange crisis. Under these circumstances, it is necessary for the middle-aged people to have well-prepared retirement plan in the sense of physical, psychological, social and economical preparedness. The study intends to find out how the occupation and activity characteristics affect the physical, psychological, social and economical preparation for their remaining old age. For the purpose, questionnaires were collected from 344 middle-aged people working in private companies, public offices and small-business owners in metropolitan and Chungcheong province. The findings are as follows: First, they were physically well-prepared, as they thought their leisure activity was more important, their occupational role was more stable and they were old and healthy. Second, Women had notion about their occupation if they kept a good relationship with their colleagues. And they were psychologically prepared well as they valued their leisure activities. Third, if they perceived their occupations as stable and had more social and leisure activities, they were socially well-prepared. Fourth, Although the level of economical preparation depends on their wealth, the occupation and activity characteristics had no effect on the economical preparation. These findings imply that the retirement preparation as a recent social issue has been influenced by the nature of occupation and activity and suggest that the definite policy and program will be required for the retirement preparation of the middle-aged people from various aspects.

An Offensive Change of Japan's Defense Strategy and Strategic Implication to the South Korea Navy: Focusing on the Japan's Amphibious Rapid Deployment Brigade Creation (일본 방위전략의 공세적 변화가 한국 해군에 주는 전략적 함의 - 일본 '수륙기동단(水陸機動團)' 창설에 대한 분석을 중심으로 -)

  • Jung, Gwang-Ho
    • Strategy21
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    • s.42
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    • pp.83-113
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    • 2017
  • After defeat in World War II, Japan's Peace Constitution committed the country to forego the acquisition of offensive military capabilities. However, in the midst of the post-cold war period, Japan began to change its security posture in line with the so-called 'normal state theory', which called for a more robust defense posture and expanded security activities. The second Abe administration promoted these security policies by issuing a National Security Strategy as well as a new National Defense Program Outline(NDPO) in 2013 and by establishing new security institutions such as the National Security Council. The Abe administration also adopted the new concept of a 'Unified Mobile Defense Force' in the 2013 which replaced the 'Dynamic Defense Force' as a new criteria for the Self-Defense Force's acquisition of military capabilities. In this new concept of military capabilities, the Ground Self-Defense Force is planning to replace existing divisions with mobile divisions and to form 'Amphibious Rapid Deployment Bridge' for the first time in 2018, which has long been taboo in Japan. Japan has experience a Marine Corps in the past. Likewise, an offensive changes in the military strategy can change the spectrum of strategy and 'Amphibious Rapid Deployment Bridge' plays a big role in this. Furthermore, Japan is increasing the Coast Guard's budget and capabilities in preparation for contingencies around the Senkaku islands (called the Diaoyu in Chinese). The South Korea navy should utilize Japan's changing security posture to deter immediate threat such as North Korea's military provocations and potential enemy threat such as China, Japan, Russia.

Preparation and Evaluation of the Properties of Al-doped Zinc Oxide (AZO) Films Deposition by Rapid Thermal Annealing (급속 열처리 방법에 의한 Al-doped Zinc Oxide (AZO) Films의 제조 및 특성 평가)

  • Kim, Sung-Jin;Choi, Kyoon;Choi, Se-Young
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.25 no.7
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    • pp.543-551
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    • 2012
  • In this study, transparent conducting Al-doped Zinc Oxide (AZO) films with a thickness of 150 nm were prepared on corning glass substrate by the RF magnetron sputtering with using a Al-doped zinc oxide (AZO), ($Al_2O_3$: 2 wt%) target at room temperature. This study investigated the effect of rapid thermal annealing temperature and oxygen ambient on structural, electrical and optical properties of Al-doped zinc oxide (AZO) thin films. The films were annealed at temperatures ranging from 400 to $700^{\circ}C$ by using Rapid thermal equipment in oxygen ambient. The effect of RTA treatment on the structural properties were studied by x-ray diffraction and atomic force microscopy. It is observed that the Al-doped zinc oxide (AZO) thin film annealed at $500^{\circ}C$ at 5 minute oxygen ambient gas reveals the strongest XRD emission intensity and narrowest full width at half maximum among the temperature studied. The enhanced UV emission from the film annealed at $500^{\circ}C$ at 5 minute oxygen ambient gas is attributed to the improved crystalline quality of Al-doped zinc oxide (AZO) thin film due to the effective relaxation of residual compressive stress and achieving maximum grain size.

Study on the Use of Sardine Meal Koji and Autolysates from Sardine Meat in Rapid Processing of Sardine Sauce (자가 소화액 및 정어리 기질 코오지를 이용한 속성 정어리 액젓 제조에 관한 연구)

  • KIM Young-Myoung;KOO Jae-Geun;LEE Young-Chul;KIM Dong-Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.2
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    • pp.167-177
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    • 1990
  • Rapid production of sardine sauce using sardine meal koji and autolysate from sardine meat was investigated. The sardine meal koji was prepared by mixing sterillized sardine meal with $10\%$ flour and $10\%$ soy sauce koji, and cultivating the mixture for 48hrs at $30^{\circ}C$ and $80\%$ R. H. For sardine autolysate preparation, chopped sardine was mixed with water (10:8= sardine:water, w/w) and autolyzed for 6hrs at $550^{\circ}C$. The optimum temperature and salinity were $40^{\circ}C$ and $15\%$ for rapid fermentation of sardine sauce. Sardine sauce were prepared experimentally under 10 kinds of conditions and fermented for 20 days. The excellent effects of sardine meal koji and autolysates from sardine meat on rapid processing of sardine sauce were showed in enhancing its flavor and advancing of nitrogenous compounds.

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Simultaneous Determination of Vitamin A and E in Infant Formula by Rapid Extraction and HPLC with Photodiode Array Detection (신속추출법 및 PDA-HPLC에 의한 조제분유 중 비타민 A, E의 동시분석)

  • Kwak, Byung-Man;Lee, Ki-Woong;Ahn, Jang-Hyuk;Kong, Un-Young
    • Korean Journal of Food Science and Technology
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    • v.36 no.2
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    • pp.189-195
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    • 2004
  • Rapid and simple method was developed for simultaneous determination of vitamins A and E contents in infant formula. Vitamins A and E were extracted by PDA-HPLC with reversed phase column using organic solvent, and their contents in Certified Reference Material (CRM) and infant formula were determined and compared with results of Food Standards Codex and AOAC method for evaluation of developed method, Vitamins A and E contents in CRM determined by developed method were within certified range of standard values. Developed method has great advantages of simple and rapid sample preparation and simultaneous determination of vitamins A and E by PDA-HPLC using reversed phase column.

Development of rapid diagnosis technology for porcine proliferative enteropathy (1) - Preparation of the samples and antibody for rapid detecting the lawsonia in pig feces - (돼지증식성회장염 신속검진 기술개발(1) - 돼지 분변에서의 로소니아균 검출을 위한 항원, 항체 준비 -)

  • Kim, Hyuck Joo;Hong, Jong Tae;Yu, Byeong Kee;Kim, Gi Young;Lee, Jin Ju;Kim, Suk
    • Journal of Biosystems Engineering
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    • v.37 no.6
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    • pp.420-428
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    • 2012
  • Purpose: Porcine proliferative enteropathy(PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. The bacterial pathogen invades the intestinal epithelial cells which causes hyperplasia of the infected cells and leads to the process of disease pathogenesis. For diagnosing PPE in a pig farm in earlier stage, a rapid diagnosing test equipment is needed for farmers. To test the equipment appropriately, we prepare the samples and antibodies for rapid detecting the Lawsonia intracellularis in pig feces. Methods : To prepare the PPE infected samples, we sampled PPE suspected pig feces in a pig farm. To manufacture a anti-Lawsonia intracellularis antibody for capturing the Lawsonia intracellularis, the rabbit-anti LsaA synthetic peptide polyclonal antibody was inoculated to rabbits. To select the couple of antibodies which is most well sandwiched with the bacteria, ELISA test was done with PPE infected ileum samples. Finally, to verify the PPE infected feces which would be used to test the rapid kit, PCR test was done on the sampled PPE suspected feces Results : The rabbit-anti LsaA synthetic peptide polyclonal antibody is developed, and is verified to capture the bacterial well through the fluorescence antibody test. Also, we found that the monoclonal antibody and the polyclonal antibody could be used as couples for sandwiching the bacteria. Finally, through the PCR test for samples of pig feces, we could prepare the 150 PPE positive samples and 50 PPE negative samples. Conclusions : The manufactured polyclonal antibody and the imported monoclonal antibody could be used to capture the bacteria using the sandwich techniques. Also, the prepared PPE infected negative and positive samples could be used to test the performance of the rapid kit to capture the bacterium Lawsonia intracellularis.

Effects of Old Age Preparation of Baby Boomers Give Dependents Awareness of Parent (베이비부머의 노후준비가 부모부양 의식에 미치는 영향)

  • Park, Keun-Soo;Kim, Tae-Il
    • The Journal of the Korea Contents Association
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    • v.16 no.2
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    • pp.467-479
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    • 2016
  • The purpose of this study is to check effects on the awareness of parents supporting in their self retirement preparation of the baby boomer generations who have faced relatively bad situation in this era considering the reality of great changes brought in the parents supporting spirit which was regarded as family and individual responsibility due to the rapid industrialization and urbanization. In the result of this study, some points such as religious, living status and monthly average earning show meaningful result and also all of the colligation in the retirement preparation standard concerning economic living life, health life, leisure time, family life, dwelling life and leisure life shows most significance probability of .001(*p<0.05) and retirement preparation standard relative to economic life indicates significance probability of .005(*p<0.05). Eventually this result shows that the higher people have retirement standard, the higher parents supporting sprit they have. So I would like to suggest some policy advices on the basis of this study. First stable supporting policy on their dwelling life should be assisted by differential methods, secondly social programs aid for leisure life through social activities like volunteer work are required, further more national based policy supports for youngsters are considered in order to reduce economic daily life on their economic activities.