• Microbiology and Biotechnology Letters
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• v.23 no.6
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• pp.781-783
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• 1995

A method is described for the rapid and simple isolation of genomic DNA from 3 ml culture of Bifidobacterium. The method is expected to be used in gene manipulation of Bifidobacterium spp. The isolated DNA using this method is shown to be an excellent substrate for restriction endonuclease digestion and ligation with T4 DNA ligase.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.136.1-136
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• 2003

Bacterial grain rot by Burkholderia gluae cause severe damage in seedling and grain of rice after heading season. This seed-borne pathogen play a role as first infection agent that could be cause disease following cropping season. Until now the direct isolation of the bacteria has some trouble by interference of other bacteria existed inside seed. This study established convenient identification method as simple isolation with KB medium from seed showing symptom and using PCR identification. By this isolation method, B. glumae was isolated from 40 to 50％ in brown rice and inner hull, however, there were saprophytic bacteria and fungi outer hull. In PCR identification with Ogf4 and Ogr3 primer to these 25 isolates, the amplified products were presented in all of the collections but not in 10 saprophytic germs. The isolation rate was constant to 3 months stored seeds. This result provide a rapid and convenient isolation and identification of B. glumae.

• Korean Journal of Veterinary Research
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• v.39 no.4
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• pp.765-771
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• 1999

Unlike most Escherichia coli strains, E coli O157 : H7 didn't ferment sorbitol within 24h of incubation and showed a negative reaction for $\beta$-glucuronidase. We developed a new medium for the rapid isolation of E coli O157 : H7 using sorbitol MacConkey agar with cefixime, potassium tellurite and 4-methylumbelliferyl-${\beta}$-D-glucuronide (MUG) as a primary plating medium. The addition of $20{\mu}g/ml$ of vancomycin in enrichment broth for E coli O157 : H7 inhibited lots of Gram positive bacteria. Three strains (10.3%) of 29 non-O157 E coli strains and 3 strains (8.3%) of 36 Salmonella spp were inhibited at the $0.05{\mu}g/ml$ of cefixime and 23 strains (79.3%) of 29 non-O157 E coli strains and 12 strains (33.3%) of 36 Salmonella spp were inhibited at the $2.0{\mu}g/ml$ of potassium tellurite. But none of the E coli O157 : H7 was affected at these concentration. The addition of MUG at $100{\mu}g/ml$ level to sorbitol MacConkey agar with cefixime and potassium tellurite (CTM-SMAC) aided in the rapid isolation of E coli O157 : H7 from samples by checking sorbitol-negative and $\beta$-glucuronidase negative phenotypes simultaneously. In conclusion, inoculation of a positive in the O157 screening test from enrichment broth on CTM-SMAC appeared to be a rapid, cost-effective and sensitive method for the isolation of E coli O157 : H7.

• Microbiology and Biotechnology Letters
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• v.43 no.4
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• pp.401-404
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• 2015

Bacillus coagulans has been considered to be a prominent candidate for probiotics as well as a thermotolerant biomaterial producer. However, the species has not attracted the attention of Korean researchers, nor has it been commercialized in Korea. Therefore, isolates for functional studies are not readily available. To secure B. coagulans resources for future applications, we developed a rapid isolation method for the species from rice straw. Introduction of the enrichment culture at $50^{\circ}C$, the selection of acid producers with $CaCO_3$ supplemented medium, and the elimination of enterococci by selective medium, rendered the successful and rapid isolation of B. coagulans strains.

• Journal of The Korea Institute of Healthcare Architecture
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• v.30 no.3
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• pp.25-33
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• 2024

Purpose: As infectious diseases spread, hospitals have converted general wards into negative pressure isolation wards through remodeling. During the conversion process, there were limitations in converting the existing ward into an effective isolation ward due to its existing structure and mechanical system. To minimize these problems, this study proposes some general ward planning methods taking into account effective conversion to an infectious disease ward. Methods: Seven rapid conversion isolation wards have been analyzed in order to check their appropriateness as a negative pressured isolation unit. Then, general ward design planning methods that can minimize problems in rapidly converted negative pressured wards have been derived. Results: If general wards can be efficiently converted into negative pressure isolation wards, many isolation facilities can be secured effectively in a short period of time during a pandemic.

• The Journal of the Korean Society for Microbiology
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• v.9 no.1
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• pp.13-18
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• 1974

Blood is one of the most important clinical specimens for the isolation of bacteria. A rapid isolation and a high isolation rate of bacteria are very important in blood culture because bacteremic patients are mostly in grave condition. Various blood culture media which support growth of most fastidious bacteria are available commercially. However, growth of bacteria are frequently delayed because of antibacterial activity of blood. Sodium polyanethol sulfonate(Liquoid) has been reported to inactivate the antibacterial substance and disrupt phagocytic cells. The beneficial effect of SPS is well recognized in the isolation of gram-positive bacteria. However, the effect does not seem to be prominent for gram-negative bacilli isolation mainly due to the rapidity of their growth. It has been experienced with Sal. typhi that the growth is much slower than that of other gram-negative bacilli. For the rapid growth of the organism, use of bile broth has been recommended. Although Sal. typhi is the most frequently isolated organism at present, about one half of total isolates are other organisms and, in case bile broth is used, other media which support growth of these organisms should be used together. Fluid thioglycollate medium(FTM) which is always used in blood culture to isolate anaerobes is inferior to brain heart infusion(BHI) for the isolation of aerobes. This study was done to determine the effect of SPS on the isolation of Sal. typhi from blood. During the Sep. 1973 to Sep. 1974 study period, 2460 blood cultures were made from the Severance hospital patients: BHI and FTM sets 1431 specimens, BHI with SPS(0.05%) and FTM sets 396 specimens, BHI and FTM with SPS sets 359 specimens, BHI and BHI with SPS sets 274 specimens. Mean incubation time required for the macroscopic detection of growth of Sal. typhi were 3.5 days on BHI and 2.7 days on BHI with SPS. The 0.8 day difference was statistically significant. On FTM the mean incubation time was 3.8 days while it was 2.9 days on FTM with SPS. The 0.9 day difference was statistically significant. The result on BHI with and without SPS sets showed faster growth on BRI with SPS in 7 specimens and slower growth in one specimen and the remaining 12 showed growth at the same time. These specimens had mean incubation time of 3.2 days on BHI and 2.3 days on BHI with SPS. The 0.9 day difference was statistically significant. This study indicates beneficial effect of SPS for the rapid isolation of Sal. typhi from clinical blood specimens.

• Proceedings of the KSME Conference
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• 2001.06e
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• pp.288-293
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• 2001

This paper presents the water-hammer effect due to the rapid opening and closing of isolation valve and thruster valve in the spacecraft propulsion system. The single propellant feed system was modeled to investigate the maximum peak pressure due to the water-hammer effect. The test parameters are tank supply pressure, shape and throat length of orifice and line length. Kerosene was used as the inert simulant propellant liquid instead of hydrazine. As downstream line length after isolation valve increased from 1.5 to 2.5m, the maximum line-filling water-hammer peak pressure decreased, but the average time interval between peak pressures increased. The maximum line-filling water-hammer peak pressure with orifice was lower than without orifice, and the maximum line-filling water-hammer peak pressure with orifice at the back of isolation valve was lower than with orifice in front of isolation valve. Without orifice, the maximum water-hammer peak pressure due to the rapid opening and closing of the thruster valve was about 126% of tank supply pressure. With orifice, it decreased. As orifice throat length increased, it decreased. The maximum water-hammer peak pressure due to the rapid closing of the thruster valve with converging-diverging orifice was lower than normal orifice. It was found that the orifice as a means of pressure drop was very effective to reduce the water hammer peak pressure at the thruster valve. The results of this study can be used for the design of spacecraft liquid propulsion feed system.

• Journal of The Korea Institute of Healthcare Architecture
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• v.29 no.4
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• pp.29-35
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• 2023

Purpose: The 2015 Middle East Respiratory Syndrome (MERS) outbreak and the recent COVID-19 pandemic have highlighted the lack of negative pressure isolation rooms and the fragility of the healthcare system. The need for healthcare facility transformation for respiratory infectious diseases has become more prominent due to COVID-19, and the purpose of this study is to provide a foundation for the rapid, economical, and safe construction of negative pressure isolation wards. Methods: This study analyzes the current status of hospitals that have been converted to negative pressure isolation rooms, and provides architectural plans and examples to provide a reference for bedroom change. Research data of this study have been obtained by analyzing the drawings of negative pressure isolation wards of nationally designated inpatient treatment beds and urgent isolation beds. In addition, the relevant literature of urgent isolation beds has been analyzed to derive bedroom change type. Result: In this study, a total of 21 isolation bed conversion methods have been presented. Implications: In order to change efficiently from a general ward to an isolation ward, it is necessary to consider the actual hospital's infectious disease transmission patterns and facility conditions.

• KSBB Journal
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• v.15 no.4
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• pp.411-413
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• 2000

A method is described for the rapid and simple isolation of genomic DNA from 3 mL culture of Lactobacillus crispatus KLB46 The isolated DNA using this method was shown to be an excellent substrate for restriction endonclease digestion and PCR. The method is expected to be used in gentic manipulation of L. crispatus KLB46.

• Journal of Applied Biological Chemistry
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• v.57 no.3
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• pp.259-265
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• 2014

Mitochondrial DNA (mtDNA)-depleted (${\rho}^0$) cells are often used as mtDNA recipients to study the interaction between the nucleus and mitochondria in mammalian cells. Therefore, it is crucial to obtain mtDNA-depleted cells with many different nuclear backgrounds for the study. Here, we demonstrate a rapid and reliable method to isolate mammalian mtDNA-depleted cells involving treatment with the antimitochondrial agents ethidium bromide (EtBr) and 2',3'-dideoxycytidine (ddC). After a short exposure to EtBr or ddC, followed by rapid clonal isolation, we were able to generate viable mtDNA-depleted cells from mouse and human cells and were able to successfully repopulate them with exogenous mitochondria from platelets isolated from mouse and human blood samples. These mtDNA-depleted cells can be used to characterize the nuclear mitochondrial interactions and to study mtDNA-associated defects in mammalian cells. Our method of isolating mtDNA-depleted cells is practical and applicable to a variety of cell types.