• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.269-273
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• 2006

The study of molecular markers to improve crops largely depends on the availability of rapid and of efficient DNA extraction methods. Here we developed a cheap and convenient method to isolate genomic DNA from rice grains suitable for large-scale microsatellite analysis. We confirmed that the isolated rice DNA is suitable for PCR analysis with STS marker and SNP marker, as well as microsatellite marker. Further, we established high-throughput DNA extraction system in a 96-well plate format which make it possible high-throughput analysis of microsatellite markers with rice grains. This implies that the new method could be a useful tool for other types of marker analysis in large scale.

• Journal of Electrical Engineering and Technology
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• v.13 no.6
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• pp.2485-2492
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• 2018

The rapid development of large-scale integrated circuits has brought great challenges to the circuit testing and diagnosis, and due to the lack of exact fault models, inaccurate analog components tolerance, and some nonlinear factors, the analog circuit fault diagnosis is still regarded as an extremely difficult problem. To cope with the problem that it's difficult to extract fault features effectively from masses of original data of the nonlinear continuous analog circuit output signal, a novel approach of feature extraction and dimension reduction for analog circuit fault diagnosis based on wavelet packet decomposition, local linear embedding algorithm, and clone selection algorithm (WPD-LLE-CSA) is proposed. The proposed method can identify faulty components in complicated analog circuits with a high accuracy above 99%. Compared with the existing feature extraction methods, the proposed method can significantly reduce the quantity of features with less time spent under the premise of maintaining a high level of diagnosing rate, and also the ratio of dimensionality reduction was discussed. Several groups of experiments are conducted to demonstrate the efficiency of the proposed method.

• The KIPS Transactions:PartB
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• v.10B no.6
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• pp.705-710
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• 2003

Recent years, as a rapid development of multimedia technology, video database system to retrieve video data efficiently seems to core technology in the oriented society. This thesis describes an efficient automatic frame detection and location method for content based retrieval of video. Frame extraction part is consist of incoming / outgoing car frame extraction and car number frame extraction stage. We gain star/end time of car video also car number frames. Frames are selected at fixed time interval from video and key frames are selected by color scale histogram and edge operation method. Car frame recognized can be searched by content based retrieval method.

• Proceedings of the KIPE Conference
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• 2001.10a
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• pp.650-655
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• 2001

Wind speeds can vary rapidly and wind turbines cannot easily follow these variations because of their inertia and aerodynamic characteristics. For maximum energy extraction. the turbine blades should operate at their optimum tip speed ratio, but with rapid changes in wind speed. this is usually not possible. To improve the energy extraction from turbulent wind, it is necessary to establish an effective measure of the high frequency component of the wind. and then to use this measure to optimise the operation of the turbine controller for maximum energy extraction. This paper presents an approach for combining readings from three anemometers into a composite wind speed measurement. and using this signal to control the operation of a permanent magnet generator to achieve maximum energy extraction. The method combines simulation and experimental investigations into a heuristic algorithm. and demonstrates its effectiveness with field trials.

• The Journal of Korean Academy of Prosthodontics
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• v.39 no.6
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• pp.633-640
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• 2001

Statement of problem. Relatively low success rate of root analogue implant system was supposed to be due to the time duration between extraction and implant installation. The use of three-dimensional computer tomography and the reconstruction of objects using rapid prototyping methods would be helpful to shorten this time. Purpose. This aim of this study was to evaluate the application possibility of the 3-dimensional computer tomography and the rapid prototyping to root analogue implants. Material and methods. Ten single rooted teeth were prepared. Width and height of the teeth were measured by the marking points. This was followed by CT scanning, data conversion and rapid prototyping model fabrication. 2 methods were used; fused deposition modelling and stereolithography. Same width and height of this models were measured and compared to the original tooth. Results. Fused deposition modelling showed an enlarged width and reduced height. The stereolithography showed more exact data compared with the fused deposition modelling. Smaller standard deviation were recorded in the stereolithographic method. Overall width error from tooth to rapid prototyping was 7.15% in fused deposition modelling and 0.2% in stereolithography. Overall height showed the tendency of reducing dimensions. Conclusion. From the results of this study, stereolithography seems to be very predictable method of fabricating root analogue implant.

• KSBB Journal
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• v.15 no.4
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• pp.411-413
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• 2000

A method is described for the rapid and simple isolation of genomic DNA from 3 mL culture of Lactobacillus crispatus KLB46 The isolated DNA using this method was shown to be an excellent substrate for restriction endonclease digestion and PCR. The method is expected to be used in gentic manipulation of L. crispatus KLB46.

• Journal of Ginseng Research
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• v.44 no.1
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• pp.44-49
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• 2020

Background: Salting-out extraction (SOE) had been developed as a special branch of aqueous two-phase system recently. So far as we know, few reports involved in extracting ginsenosides with SOE because of the lower recovery caused by the unique solubility and surface activity of ginsenosides. A new SOE method for rapid pretreatment of ginsenosides from the enzymatic hydrolysates of Panax quinquefolium was established in this article. Methods: The SOE system comprising ethanol and sodium carbonate was selected to extract ginsenosides from the enzymatic hydrolysates of Panax quinquefolium, and HPLC was applied to analyze the ginsenosides. Results: The optimized extraction conditions were as follows: the aqueous two-phase extraction system comprising ethanol, sodium carbonate, ethanol concentration of 41.51%, and the mass percent of sodium carbonate of 7.9% in the extraction system under the experimental condition. Extraction time had minor influence on extraction efficiency of ginsenosides. The results also showed that the extraction efficiencies of three ginsenosides were all more than 90.0% only in a single step. Conclusion: The proposed method had been successfully applied to determine ginsenosides in enzymatic hydrolysate and demonstrated as a powerful technique for separating and purifying ginsenosides in complex samples.

• Analytical Science and Technology
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• v.37 no.2
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• pp.123-129
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• 2024

We present a rapid method for the determination of Cs, Sr, and Ba, heat generators found in highly active liquid wastes, by gas-pressurized extraction chromatography (GPEC) using a column containing a cation-exchange resin. GPEC is a microscale column chromatographic technique that uses a constant flow rate of solvent (0.07 mL/min) with pressurized nitrogen gas supplied through a valve. In particular, because this method uses a small sample volume (a few hundred microliters), it produces less chemical waste and allows for faster separation compared to traditional column chromatography. In this study, we evaluated the separation of Cs, Sr, and Ba using GPEC. The eluate from the column (GPEC or conventional column chromatography) was quantitatively analyzed using inductively coupled plasma-mass spectrometry to measure the column recovery and precision. The column reproducibility of the proposed GPEC system (RSDs of recoveries) ranged from 2.7 to 4.1 %, and the column recoveries for the three elements ranged from 72 to 98% when aqueous HCl was used as the eluent. The GPEC results are slightly different in efficiency and separation resolution compared to those of conventional column chromatography because of the differences in the eluent flow rate as well as the internal diameter and length of the column. However, the two methods had similar recoveries for Cs and Sr, and the precision of GPEC was improved by two-fold. Remarkably, the solvent volume required for GPEC analysis was five times lower than that of the conventional method, and the total analysis time was 11 times shorter.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.595-602
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• 2005

A one-step real-time quantitative RT-PCR assay in combination with automated RNA extraction was evaluated for routine testing of HCV RNA in the laboratory. Specific primers and probes were developed to detect 302 bp on 5'-UTR of HCV RNA. The assay was able to quantitate a dynamic linear range of $10^7-10^1$ HCV RNA copies/reaction ($R^2=0.997$). The synthetic HCV RNA standard of $1.84{\pm}0.1\;(mean{\pm}SD)$ copies developed in this study corresponded to 1 international unit (IU) of WHO International Standard for HCV RNA (96/790 I). The detection limit of the assay was 3 RNA copies/reaction (81 IU/ml) in plasma samples. The assay was comparable to the Amplicor HCV Monitor (Monitor) assay with correlation coefficient r=0.985, but was more sensitive than the Monitor assay. The assay could be completed within 3 h from RNA extraction to detection and data analysis for up to 32 samples. It allowed rapid RNA extraction, detection, and quantitation of HCV RNA in plasma samples. The method provided sufficient sensitivity and reproducibility and proved to be fast and labor-saving, so that it was suitable for high throughput HCV RNA test.

• Analytical Science and Technology
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• v.24 no.4
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• pp.266-274
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• 2011

Using a Hitachi LaChrom Ultra 2000U, a reverse phase ultra high performance liquid chromatography (u-HPLC) method was developed for the rapid quantification of 14 PAHs in foods. The proposed method for PAH analysis is based on solid phase extraction (SPE) cartridges; the determination was carried out by u-HPLC with fluorimetric detection. The method was very sensitive; PAH concentration levels were in a low ${\mu}g$/kg range and could be detected and quantified. Six samples of food were analyzed. Among PAHs, PHE was found in most of samples, the concentration ranging from 2.5 to 19.9 ${\mu}g$/kg. The contents of benzo[c]fluorine (BCL), pyrene (PYR), benzo[a]anthracene (BaA), chrysene (CHR), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF) were low at the '${\mu}g$/kg' level or were less than LOD.