• Korean Journal of Environmental Biology
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• v.21 no.1
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• pp.77-85
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• 2003

We have examined the 16S-23S rRNA intergenic spacer regions (ISR) of Vibrio fluvialis. ISRs were PCR amplified, cloned into a plasmid vector and then sequenced. As results of ISR nucleotide sequence analysis, total of 6 clones were isolated depending on the size. The clones were different in both the number and the composition of the tRNA genes, and were designated ISR-A, ISR-E, ISR-El, ISR-lA, ISR-EKV, ISR-EKAV. ISR-A contains $tRNA^{Ala}$; ISR-lA, $tRNA^{Ile}$-$tRNA^{Ala}$; ISR-EKV, $tRNA^{GIu}$-$tRNA^{Lys}$-$tRNA^{Val}$;ISE-EKAV, $tRNA^{GIu}$-$tRNA^{Lys}$-$tRNA^{Ala}$-$tRNA^{Val}$; ISR -E and E1, $tRNA^{GIu}$ clusters. ISR-EKV was shown to be a minor type out of the six ISR types and showed a very limited homology between ISR-EKV from V, fluvialis and ISRa from other Vibrio species. Therefore ISR-EKV sequence was used to design species-specific primers to detect V, fiuvialis from other Vibrio species by PCR reaction. The specificity of the primers was examined using genomic DNA of other Vibrios as templates for PCR reaction. The result showed that PCR can be a useful method to detect V. fluvialis among Vibrio species in a single PCR reaction.

• The Journal of Korean Society of Virology
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• v.26 no.1
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• pp.9-22
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• 1996

Respiratory Syncytial virus (RSV) is an important cause of acute lower respiratory tract infections in human, with infants and young children being particularly susceptible. In the temperate zones, sharp annual outbreaks of RSV occur during the colder months, in both the northern and the southern hemisphere. RSV is unusual in that it can repeatedly reinfect individuals throughout life and infect babies in the presence of maternal antibody. RSV isolates can be divided into two subgroups, A and B, on the basis of their reactions with monoclonal antibodies, and the two subgroups are also distinct at the nucleotide sequence level. The specific diagnosis of RSV infection was best made by isolation of virus in tissue culture, identification of viral antigen, or by specific serologic procedures. Recently, rapid detection of RSV and analysis of RSV strain variation became possible by development of methods of reverse transcription and polymerase chain reaction amplification. In this study, to determine the genetic diversity of RSV found in Korea, 173 bp and 164 bp spanning selected regions of the RSV F and SH genes were enzymatically amplified and sequenced, respectively. Eight for F gene and three for SH gene were detected in 66 nasopharyngeal swap samples tested. Two major antigenic subgroups, A and B were confirmed from Korean samples (seven for subgroup A and one for subgroup B). At the nucleotide level of the F gene region, Korean subgroup A strains showed 95-99% homologies compared to the prototype A2 strain of subgroup A and 93-100% homologies among Korean subgroup A themselves. For the SH gene region, Korean subgroup A strain showed 97.5% homology compared to the prototype A2 strain of subgroup A, and Korean subgroup B strain showed 97% homology compared to the prototype 18537 strain of subgroup B. Most of base changes were transition and occured in codon position 3, which resulted in amino acid conservation. Using the maximum parsimony method, phylogenetic analysis indicated that Korean RSV strains formed a group with other RSV strains isolated from the United States, Canada, the Great Britain and Australia.

• Korean Security Journal
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• no.46
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• pp.63-86
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• 2016

The object of this study is to propose a study on the Private Investigator usage for Cyber Crime. The latest trend of cyber crime is being evolve in sophisticated and complex way over the global, like internet fraud, cyber gambling, hacking and etc. Hence national investigative authority mobilize high specialized skills and method of criminal investigation by each nation. But it is hard to respond in rapid and effective way because of propoor, distribution of group and insufficient of related legal system. Already in other countries, not considerable amount of services are given to private investigators in detection and tracking part which is inefficient by nation. So it has significantly meaningful to compensate the defect and study about private investigator usage as companion of cooperation policing for effectively respond to cyber-crime. The way to effectively deal with the cyber-crime is reevaluate meaning of partnership policing and need of private investigator usage. Also it is to analyze the main issue about introduction of a system and suggest the effective way of introduction. First, legislation of private investigator usage which is based upon partnership policing should be made up. Moreover, to establish the range of private investigator's business and enhance the reliability, it is to propose introduction of leading professional global certificate and license system with sufficient education and test. We are expecting introduction of private investigator usage can improve efficiency of investigation and promote effective countermeasures of cyber-crime.

• Journal of Advanced Navigation Technology
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• v.16 no.2
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• pp.360-366
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• 2012

In the power distribution panel installed in airport or industrial facilities, MCCB has been used for main switch and ELCB for branch switch to perform human body and leakage-inducing fire protection as well as overcurrent and short circuit protection. Especially for the airport panel, increase in accident protection is needed for stable power supply due to rapid modernization with fast-growing users, higer capacity and diversification of equipment, the increase of power capacity and the breaker made slim is a main issue for now because the issue for installation space is standing out by making panel with two-row arrangement connection method, etc. due to a many use of branch ELCBs. In this thesis, we designed arc extinguishing mechanism, considered movement direction change of contact in mechanism design. Also, we designed the breaker to work stably in case of miniaturization of leakage detection circuit and reverse connection. We conducted short circuit test to verify its function and developed the breaker that can be improved protection against accidental current with slim size operating leakage function when reverse connection to help solve the problem in using space that is increasing in the airport distribution panel.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.11
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• pp.5879-5884
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• 2013

With the rapid industrialization and economical development, the electricity demands of the industrial facilities and densely populated large cities are continuing to increase in Korea. The increase in the power consumption requires the extension of power facilities, but it is difficult to secure spaces for equipment installation in the limited space of urban areas. In addition, the 154 kV or 345 kV transmission systems in Korea has a short transmission distance, and are connected to one another in network structures that ensure the high reliability and stability of power supply. This structure reduces the impedance during the fault in power system, and increases the magnitude of in the short circuit fault current. The superconducting fault current limiter (SFCL) was devised to effectively address these existing problems. The SFCL is a new-concept eco-friendly protective device that ensures fast operation and recovery time for the fault current and does not need additional fault detection devices. Therefore, many studies are being conducted around the world. In this paper, based on the wiring method the initial fault current characteristics, current limiting characteristics, according to the incident angle and the change in inductance current limiting characteristics were analyzed in a multifaceted methods.

• Food Science of Animal Resources
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• v.34 no.6
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• pp.799-807
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• 2014

Meat source fraud and adulteration scandals have led to consumer demands for accurate meat identification methods. Nucleotide amplification assays have been proposed as an alternative method to protein-based assays for meat identification. In this study, we designed Loop-mediated isothermal amplification (LAMP) assays targeting species-specific mitochondrial DNA to identify and discriminate eight meat species; cattle, pig, horse, goat, sheep, chicken, duck, and turkey. The LAMP primer sets were designed and the target genes were discriminated according to their unique annealing temperature generated by annealing curve analysis. Their unique annealing temperatures were found to be $85.56{\pm}0.07^{\circ}C$ for cattle, $84.96{\pm}0.08^{\circ}C$ for pig, and $85.99{\pm}0.05^{\circ}C$ for horse in the BSE-LAMP set (Bos taurus, Sus scrofa domesticus and Equus caballus); $84.91{\pm}0.11^{\circ}C$ for goat and $83.90{\pm}0.11^{\circ}C$ for sheep in the CO-LAMP set (Capra hircus and Ovis aries); and $86.31{\pm}0.23^{\circ}C$ for chicken, $88.66{\pm}0.12^{\circ}C$ for duck, and $84.49{\pm}0.08^{\circ}C$ for turkey in the GAM-LAMP set (Gallus gallus, Anas platyrhynchos and Meleagris gallopavo). No cross-reactivity was observed in each set. The limits of detection (LODs) of the LAMP assays in raw and cooked meat were determined from $10pg/{\mu}L$ to $100fg/{\mu}L$ levels, and LODs in raw and cooked meat admixtures were determined from 0.01% to 0.0001% levels. The assays were performed within 30 min and showed greater sensitivity than that of the PCR assays. These novel LAMP assays provide a simple, rapid, accurate, and sensitive technology for discrimination of eight meat species.

• Journal of Life Science
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• v.15 no.6 s.73
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• pp.879-883
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• 2005

This study was differentiated between Korea and China arkshells using PCR-aided RFLP method which could identify the variation for inter-and intra-species of arkshell (Scapharca broughtonii Schrenck) at the level of DNA. The DNA fragment patterns were compared after digesting gene of mitochondrial 16S rDNA with 8 kinds of restriction enzymes. A 720 bp DNA fragment corresponding to 16S rDNA gene was amplified by PCR with primers ArkF-3 and ArkR-3. PCR products were cut by restriction enzymes (Pvull, BamHI, Hinfl, HaeIII, EcoRI, RsaI, Ksp221, and BstX21), and RFLP pattern was studied. A unique 275 bp DNA band was observed in the samples from Dukyang, Gamak, Namhae, Jinhae, and Taean in Korea when treated by Hinfl, but Chinese arkshell did not show. Treatment of HaeIII could discriminate the sample of Namhae and Jinhae from Dukyang/Gamak/Taean, as well as Korean and Chinese arkshell based on a 700 bp. However, PuvII, BamHI, EcoRI, RsaI, Ksp221, and BstX21 showed the same of 700 bp band in Korean and Chinese arkshell. The phylogenetic tree inferred from PCR-RFLP pattern comparsion in Korean arkshell was different that the distance between Dukyang/Gamak/Taean and Namhae/Jinhae was approximately 7. In particular, the distance between Korean and Chinese arkshell was 25. Consequently, HinfI and HaeIII played an important role in a reliable molecular tool for rapid discriminating Korean and Chinese arkshell, as well as a intra-species in Korea.

• Journal of the Korean earth science society
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• v.40 no.5
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• pp.502-517
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• 2019

Global warming and rapid climate change have long affected the characteristics of typhoons in the Northwest Pacific, which has induced increasing devastating disasters along the coastal regions of the Korean peninsula. Synthetic Aperature Radar (SAR), as one of the microwave sensors, makes it possible to produce high-resolution sea surface wind field around the typhoon under cloudy atmospheric conditions, which has been impossible to obtain the winds from satellite optical and infrared sensors. The Geophysical Model Functions (GMFs) for sea surface wind retrieval from SAR data requires the input of wind direction, which should be based on the accurate estimation of the center of the typhoon. This study estimated the typhoon centers using Sentinel-1A images to improve the problem of typhoon center detection method and to reflect it in retrieving the sea surface wind. The results were validated by comparing with the typhoon best track data provided by the Korea Meteorological Administration (KMA) and Japan Meteorological Agency (JMA), and also by using infrared images of Himawari-8 satellite. The initial center position of the typhoon was determined by using VH polarization, thereby reducing the possibility of error. The detected center showed a difference of 23.76 km on average with the best track data of the four typhoons provided by the KMA and JMA. Compared to the typhoon center estimated by Himawari-8 satellite, the results showed an average spatial variation of 11.80 km except one typhoon located near land with a large difference of 58.73 km. This result suggests that high-resolution SAR images can be used to estimate the center and retrieve sea surface wind around typhoons.

• 재활복지
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• v.18 no.4
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• pp.221-235
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• 2014

Recently, the frequency of stroke disease is increased due to the rapid aging population, and is contributed to the major occurrence factors of the posteriori acquired disability. This study is about an postural change device for the control of supine posture which is an assisted equipment using in daily rehabilitation process for overcoming the disability by the aftereffects of the stoke disease. In this paper, the existing domestic and Japan postural appliances is examined and its comparison and categorization is performed according to its functions and purposes. Here, in order to control the supine posture state, the design method for advanced multi functional system is proposed, which is devised to have an unified mattress control operations of combining the bedsore prevention tube with the supine posture tilting tube. And also, in addition of an smart function, it is designed to enable to perform an RF functions such as the monitoring of the present device state, the alteration of the basic position and the control of alternative floating and supine posture. This system control hardware consists of three main parts : the sensor detection part, the motor driving /control part, and the system control part for bluetooth communication. In results, we confirmed that the system designed by this research is possible to make it practical as an advanced smart postural change device combined by IoT technology in the application field of the recent IT technology.

• Analytical Science and Technology
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• v.19 no.5
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• pp.383-388
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• 2006

Simple post-wash method by ion chromatography (IC) was established for the rapid and precise determination of fluoride ion in wastewater from mine in fluorite mineralized area. High sulfate in sample was retained in a pre-column and less strongly held fluoride ion was transferred to the principal separation system using modified conventional IC with switching technique. An analytical column with high capacity (AS 9 HC) was used as a pre-column to retain the amount of high sulfate. A guard column (AG 14) as a separation column was used to increase the response of fluoride and reduce the system pressure. According to the recovery of fluoride ion with one detector and the observation of sulfate peak with another conductivity detector, the optimum switching time of 10-port chromatographic injector was 4.3 min. The limit of detection (S/N = 3) of fluoride in synthetic solution containing $500mg\;L^{-1}$ sulfate was $2.4{\mu}g/L$, with $25{\mu}L$ sample volume.