• Food Science of Animal Resources
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• v.42 no.6
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• pp.1061-1073
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• 2022

Receptor activator of NF-kB ligand (RANKL) is known to play a major role in bone metabolism and the immune system, and its recombinant form has been expressed in bacterial systems for research since the last two decades. However, most of these recombinant forms are used after purification or directly using living cells. Here, there were cell extracts of recombinant Lactococcus lactis expressing mouse RANKL (mRANKL) used to evaluate its biological activity in mice. Mice were divided into three groups that were fed phosphate-buffered saline (PBS), wild-type L. lactis IL1403 (WT_CE), and recombinant L. lactis expressing mRANKL (mRANKL_CE). The small intestinal transcriptome and fecal microbiome were then profiled. The biological activity of mRANKL_CE was confirmed by studying RANK-RANKL signaling in vitro and in vivo. For small intestinal transcriptome, differentially expressed genes (DEGs) were identified in the mRANKL_CE group, and no DEGs were found in the WT_CE group. In the PBS vs. mRANKL_CE gene enrichment analysis, upregulated genes were enriched for heat shock protein binding, regulation of bone resorption, and calcium ion binding. In the gut microbiome analysis, there were no critical changes among the three groups. However, Lactobacillus and Sphingomonas were more abundant in the mRANKL_CE group than in the other two groups. Our results indicate that cell extracts of mRANKL_CE can play an effective role without a significant impact on the intestine. This strategy may be useful for the development of protein drugs.

• Clinical and Experimental Reproductive Medicine
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• v.50 no.3
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• pp.206-212
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• 2023

Objective: The aim of the present study was to evaluate the associations between hematologic parameters related to systemic inflammation and insulin resistance-associated metabolic parameters in women with polycystic ovary syndrome (PCOS). Methods: Eighty-two women between the ages of 18 and 35 years who were diagnosed with PCOS were included in this study. A 2-hour 75-g oral glucose tolerance test (OGTT) was administered to all study participants; fasting and postprandial glucose and insulin levels were measured simultaneously during the 2-hour OGTT. Hematologic parameters were derived from a standard complete blood count and a differential count of fasting-state blood samples. The correlations between hematologic parameters and insulin resistance-associated clinical and metabolic parameters were evaluated using the Spearman rank correlation and partial correlation coefficients. Hematologic parameters related to systemic inflammation were compared between the two groups, categorized by the presence or absence of insulin resistance. Results: Significant differences in the absolute neutrophil count, absolute monocyte count, platelet count, and neutrophil-lymphocyte ratio were found between the insulin-resistant group and insulin-nonresistant group. Correlation analysis found that all hematological parameters, except for the platelet-lymphocyte ratio, were associated with at least one insulin resistance-associated metabolic parameter. However, these significant correlations between hematological and metabolic parameters were attenuated after controlling for the effects of other covariates using partial correlation analysis. Conclusion: The association between hematologic parameters indicative of systemic inflammation and insulin resistance-associated metabolic parameters seems to be strongly influenced by other anthropometric covariates in women with PCOS.

• International conference on construction engineering and project management
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• 2022.06a
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• pp.269-276
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• 2022

The robustness of a supply chain (i.e., the ability to cope with external and internal disruptions and disturbances) becomes more critical in ensuring the success of a construction project because the supply chain of today's construction project includes more and diverse suppliers. Previous studies indicate that topological features of the supply chain critically affect its robustness, but there is still a great challenge in characterizing and quantifying the impact of network topological features on its robustness. In this context, this study aims to identify network measures that characterize topological features of the supply chain and evaluate their impact on the robustness of the supply chain. Network centrality measures that are commonly used in assessing topological features in social network analysis are identified. Their validity in capturing the impact on the robustness of the supply chain was evaluated through an experiment using randomly generated networks and their simulations. Among those network centrality measures, the PageRank centrality and its standard deviation are found to have the strongest association with the robustness of the network, with a positive correlation coefficient of 0.6 at the node level and 0.74 at the network level. The findings in this study allows for the evaluation of the supply chain network's robustness based only on its topological design, thereby enabling practitioners to better design a robust supply chain and easily identify vulnerable links in their supply chains.

• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1162-1169
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• 2023

16S rRNA short amplicon sequencing-based microbiota profiling has been thought of and suggested as a feasible method to assess food safety. However, even if a comprehensive microbial information can be obtained by microbiota profiling, it would not be necessarily sufficient for all circumstances. To prove this, the feasibility of the most widely used V3-V4 amplicon sequencing method for food safety assessment was examined here. We designed a pathogen (Vibrio parahaemolyticus) contamination and/or V. parahaemolyticus-specific phage treatment model of raw oysters under improper storage temperature and monitored their microbial structure changes. The samples stored at refrigerator temperature (negative control, NC) and those that were stored at room temperature without any treatment (no treatment, NT) were included as control groups. The profiling results revealed that no statistical difference exists between the NT group and the pathogen spiked- and/or phage treated-groups even when the bacterial composition was compared at the possible lowest-rank taxa, family/genus level. In the beta-diversity analysis, all the samples except the NC group formed one distinct cluster. Notably, the samples with pathogen and/or phage addition did not form each cluster even though the enumerated number of V. parahaemolyticus in those samples were extremely different. These discrepant results indicate that the feasibility of 16S rRNA short amplicon sequencing should not be overgeneralized in microbiological safety assessment of food samples, such as raw oyster.

• Journal of East-West Nursing Research
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• v.29 no.2
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• pp.106-116
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• 2023

Purpose: The purpose of this study was to identify the impact of obesity on the incidence of diabetes mellitus in adults with pre-diabetes. Methods: This study employed a longitudinal study design and utilized secondary data drawn from the Korean Genome and Epidemiology Study. This study used data from a sample of 3,693 adults with prediabetes who were followed every two years from 2001 to 2018. Statistical data analysis for frequency, number of cases per 1,000 person-years, log-rank test, Kaplan-Meier curve, and Cox's proportional hazards regression analysis was performed using IBM SPSS statistics version 26. Results: During the observation period, there were 1,309 (35.4%) patients with diabetes, and the total number of person-years was 35,342. The incidence of diabetes was higher in the obese group compared to the normal weight group (body mass index [BMI]: hazard ratio=1.57, 95% confidence interval [CI]=1.40~1.77, waist: hazard ratio=1.55, 95% CI=1.38~1.76, waist to hip ratio [WHR]: hazard ratio=1.53, 95% CI=1.24~1.89, body fat [BF] (%): hazard ratio=1.42, 95% CI=1.27~1.61). Conclusion: An increase in BMI, waist circumference, and WHR, which are indicators of obesity, can exacerbate the risk factors for diabetes. Thus, a decrease in BMI, waist circumference, and WHR is necessary to prevent pre-diabetes. In particular, health care professionals should provide individualized weight management program interventions, including adult obesity programs and obesity counseling in partnership with local health departments, to reduce BMI and waist circumference in people at high risk for diabetes.

• Korean Journal of Culture and Social Issue
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• v.8 no.1
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• pp.61-77
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• 2002

This study focuses on drivers' risk perception & construct in risk scene. The measures used were the scores of hazard perception, namely the subjects' evaluation of the degree of risk through the 'Q-sorting' with 30 drivers. The subjects were divided into 3 groups according to their evaluating score, Z-score, road users' hazard(type 1), environmental hazard(type 2), situational hazard(type 3). And ten constructs derived from Q-sorting were compared through 'consensus item analysis'. It suggest that there are different in constructs for risk perception. Then those results are discussed in terms of theoretical and practical implication of traffic safety including accidents analysis and drivers' education.

• Journal of Korean Academy of Nursing
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• v.54 no.1
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• pp.18-31
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• 2024

Purpose: This study aimed to investigate the impact of anthropometric indices of obesity (body mass index [BMI], waist circumference, waist hip ratio, and body fat percentage) on the incidence of hypertension in adults with prehypertension. Methods: A longitudinal study design using secondary data form the Korean Genome and Epidemiology Study was employed. The study included 1,838 adults with prehypertension tracked every two years from 2001 to 2018. Statistical analyses, including frequency assessments, number of cases per 1,000 person-years, log-rank tests, Kaplan-Meier curves, and Cox's proportional hazards regression, were conducted using SPSS version 25. Results: Over the observation period (15,783.6 person-years), 1,136 individuals developed hypertension. The incidence of hypertension was significantly higher in the obesity groups defined by BMI (hazard ratio [HR] = 1.33), waist circumference (HR = 1.34), waist hip ratio (HR = 1.29), and body fat percentage (HR = 1.31) compared to the non-obese group. These findings indicate an increased risk of hypertension associated with obesity as measured by these indices. Conclusion: The study underscores the importance of avoiding obesity to prevent hypertension in individuals with prehypertension. Specifically, BMI, waist circumference, waist hip circumference, and body fat percentage were identified as significant risk factors for hypertension. The results suggest the need for individualized weight control interventions, emphasizing the role of health professionals in addressing the heightened hypertension risk in this population.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.27 no.1
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• pp.17-29
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• 2024

This study was conducted to select the restoration priority of forest damage sites in Gangwon Province. We first identified the status of damaged areas. We then selected restoration evaluation indicators through a literature review. We then set weights for these indicators through expert surveys. We next acquired data that can represent these indicators and spatially mapped them. Finally, we prioritized the restoration target sites by taking the weights. The results of the study showed that disaster sensitivity and ecologicality are important criteria for selecting the restoration priority of damage sites. The analysis showed that damage sites in Doam, Jeongseon, Samcheok and Inje are in urgent need of restoration. The results of this study are significant in that they selected the restoration priority of damage sites in Gangwon Province based on the restoration priority evaluation criteria selected based on expert surveys. However, the priority restoration areas derived from the results of this study are not actually implementing restoration projects at present. Therefore, it is judged that it would be efficient in various aspects to establish the restoration priority area based on scientific analysis techniques and carry out the project for efficient implementation of the restoration project. In this study, it can be pointed out that the priority of restoration of damage sites was derived based on data from the past due to the limitation of data acquisition. However, the fact that the priority restoration area inferred based on past data has been restored over time has improved the reliability of the study by verifying the usefulness of the priority extraction technique. In the future, if the priority of damage sites is extracted by extracting the restoration target area boundary through the latest data based on the methodology applied in this study, it is considered that it will be available as a result that can be applied to the field.

• Annals of Hepato-Biliary-Pancreatic Surgery
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• v.27 no.3
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• pp.251-257
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• 2023

Backgrounds/Aims: This is a retrospective analysis of whether the 8th edition American Joint Committee on Cancer (AJCC) was a significant improvement over the 7th AJCC distal extrahepatic cholangiocarcinoma classification. Methods: In total, 111 patients who underwent curative resection of mid-distal bile duct cancer from 2002 to 2019 were included. Cases were re-classified into 7th and 8th AJCC as well as clinicopathological univariate and multivariate, and Kaplan-Meier survival curve and log rank were calculated using R software. Results: In patient characteristics, pancreaticoduodenectomy/pylorus preserving pancreaticoduodenectomy had better survival than segmental resection. Only lymphovascular invasion was found to be significant (hazard ratio 2.01, p = 0.039) among all clinicopathological variables. The 8th edition AJCC Kaplan Meier survival curve showed an inability to properly segregate stage I and IIA, while there was a large difference in survival probability between IIA and IIB. Conclusions: The 8th distal AJCC classification did resolve the anatomical issue with the T stage, as T1 and T3 showed improvement over the 7th AJCC, and the N stage division of the N1 and N2 category was found to be justified, with poorer survival in N2 than N1. Meanwhile, in TMN staging, the 8th AJCC was able differentiate between early stage (I and IIA) and late stage (IIB and III) to better explain the patient prognosis.

• Journal of Society of Preventive Korean Medicine
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• v.27 no.3
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• pp.1-12
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• 2023

Objectives : The aim of this study is to analyze the news articles related to Korean Medicine(KM) and compare trends in news reports from 2018 to 2022. Method : News articles related to KM were collected through the BigKinds, the news bigdata service of the Korea Press Foundation. News reports from 1 January 2018 to 31 December 2022 were searched. 2,950 news articles out of a total of 12,497 met the inclusion criteria. First, quantitative changes in media coverage were analyzed by year, media outlet, and month. For qualitative analysis, two authors independently coded the content of news articles, discussed them until consensus, and consulted with a third researcher to classify them. In addition, keywords extracted by the BigKind's Topic Rank algorithm were compared and analyzed in each year. Results : The number of news articles on KM decreased by 42% in 2022 compared to 2018. Over a fiveyear period, the Naeil Shinmun reported the most on KM among newspapers, while the Hankyoreh did the least. Among broadcasters, YTN reported the most and SBS did the least. When analyzing the reports by category, the most common was 'treatment', followed by 'prevention' and 'scientification'. As a result of extracting keywords with high weight and frequency, 'immunity' and 'immune system' ranked the first and second in 2018, while 'COVID 19' and 'medical law violation' did in 2022. Conclusion : The decrease in media reports on KM during the COVID-19 epidemic period seems to be due to the limited role of KM in responding to infectious diseases, and efforts to expand the scope of KM can induce increased media reports and social interest.