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Temperature and CO2 Level Influence Potato leafroll virus Infection in Solanum tuberosum

  • Chung, Bong Nam;Koh, Sang Wook;Choi, Kyung San;Joa, Jae Ho;Kim, Chun Hwan;Selvakumar, Gopal
    • The Plant Pathology Journal
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    • v.33 no.5
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    • pp.522-527
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    • 2017
  • We determined the effects of atmospheric temperature ($10-30{\pm}2^{\circ}C$ in $5^{\circ}C$ increments) and carbon dioxide ($CO_2$) levels ($400{\pm}50ppm$, $540{\pm}50ppm$, and $940{\pm}50ppm$) on the infection of Solanum tuberosum cv. Chubaek by Potato leafroll virus (PLRV). Below $CO_2$ levels of $400{\pm}50ppm$, the PLRV infection rate and RNA content in plant tissues increased as the temperature increased to $20{\pm}2^{\circ}C$, but declined at higher temperatures. At high $CO_2$ levels ($940{\pm}50ppm$), more plants were infected by PLRV at $30{\pm}2^{\circ}C$ than at 20 or $25{\pm}2^{\circ}C$, whereas PLRV RNA content was unchanged in the $20-30{\pm}2^{\circ}C$ temperature range. The effects of atmospheric $CO_2$ concentration on the acquisition of PLRV by Myzus persicae and accumulation of PLRV RNA in plant tissues were investigated using a growth chamber at $20{\pm}2^{\circ}C$. The M. persicae PLRV RNA content slightly increased at elevated $CO_2$ levels ($940{\pm}50ppm$), but this increase was not statistically significant. Transmission rates of PLRV by Physalis floridana increased as $CO_2$ concentration increased. More PLRV RNA accumulated in potato plants maintained at 540 or $940{\pm}50ppm$ $CO_2$, than in plants maintained at $400{\pm}50ppm$. This is the first evidence of greater PLRV RNA accumulation and larger numbers of S. tuberosum plants infected by PLRV under conditions of combined high $CO_2$ levels ($940{\pm}50ppm$) and high temperature ($30{\pm}2^{\circ}C$).

Characteristics of Tamarix chinensis Population in Shiwha Lake (시화호 내 위성류(Tamarix chinensis) 개체군의 특성)

  • Min, Byeong-Mee;Yi, Dong-Hoon;Lee, Hye-Won;Choi, Jong-In
    • The Korean Journal of Ecology
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    • v.28 no.5
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    • pp.327-333
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    • 2005
  • For the first time in Korea, plant size, distribution pattern and root growth form of Tamarix chinensis were surveyed at natural stand in Shiwha Lake, from June to July in 2005. T, chinensis grew in the range of 350 m towards north-south and 270 m in east-west direction. The mean number of stems germinated from same root system was 1.9. On the stem base, the numbers of living and dead plants were 1,398 and 114, respectively. Mean perimeter and height of 1,398 plants were $5.9{\pm}3.2$ cm and $159{\pm}51$ cm, respectively. By dividing into 10 classes based on the perimeter size, the largest number of plants were included in the second smallest class category. However, by dividing into 10 classes on the bases of plant height, variation in the number of plants along the height showed normal distribution curve. The heights of dead plants were small in perimeter but diverse in height. On the root base, the number of plants was the greatest in the smallest size class, and conspicuously decreased with size increment. Distribution of T. chinensis was clumped pattern. Age of the largest plant was 8-year. T. chinensis had two types of root growth. One was horizontal growth in the periphery of soil surface, and the other vertical growth.

Codon usage analysis of rice glutelin genes (쌀 저장 단백질 글루텔린 유전자 암호 분석)

  • Shin, Yun-Cheol;Kim, Ju-Kon;Nahm, Baek-Hie
    • Applied Biological Chemistry
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    • v.36 no.6
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    • pp.517-524
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    • 1993
  • To characterize glutelins, the most abundant storage protein in rice, 13 complete coding sequences of glutelin genes from the database were analyzed. According to the phylogenic analysis, these genes could be classified into 5 groups, Group I to V. The degrees of homology were calculated to be in the range of 90 to 60%, but the patterns of hydrophobicity were similar in all the groups. Also, each group was found to have similar amino acid composition with variations in lysine content from 2.5 to 3.6% due to the point mutation of arginine to lysine. The isoelectric points of mature proteins and their basic chains of all the groups showed the value of about 9.0 and 10.0, respectively, while the isoelectric points of acidic chains in these groups showed the distinct value of 6.6, 6.7, 7.2, 8.4 and 7.9. The plot of the fraction of G+C at synonimous site in codons (GC3s) against effective codon numbers suggest no major difference in translational efficiency in the expression of glutelin multigenes.

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Antioxidant Activity and Cytotoxicity on Human Cancer Cells of Anthocyanin Extracted from Black Soybean (검정콩 안토시아닌의 항산화 및 암세포독성)

  • Kim, Yong-Ho;Kim, Dong-Seon;Woo, Sung-Sick;Kim, Hyun-Hee;Lee, Young-Sang;Kim, Hee-Seon;Ko, Kwang-Oh;Lee, Seuk-Ki
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.53 no.4
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    • pp.407-412
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    • 2008
  • Anthocyanin pigments in soybean seed coat were D3G (Delphinidin-3-glucoside), C3G (Cyanidin-3- glucoside) and Pt3G (Petunidin-3-glucoside), which have been known potential roles in the prevention and treatment of chronic diseases. Anthocyanin contents in seed coat of blck soybean were significantly different according to soybean variety, C3G content showed the highest value in all materials and its variation was also wide. Antioxidant activity of each pigment was analyzed by DPPH and TEAC methods in which D3G and C3G showed high activity. And this study was carried out to investigate the effects of anthocyanin to human cancer cells. Cytotoxity were analyzed by MTT assay after anthocyanin pigments treated on leukemia (Jurkat T) and adenocarsinoma (MCF-7) cells. It showed decrement of cell numbers as anthocyanin concentration is increasing. ${EC}_50$ range of anthocyanin concentrations were $100{\sim}250\;ug/mL$ and $100{\sim}250\;ug/mL$ in Jurkat T and MCF-7 cell, respectively. D3G showed higher cytotoxicity than other pigments in Jurkat T cell whereas activity of C3G was high in MCF-7 cell. It is believed that supplementation of human diets with soybean anthocyanin markedly reduces human cancer mortality rates.

Absorption Characteristics of Sulfur Dioxide in Jet Bubbling Reactor (가스분사반응기에서의 SO2 흡수 특성)

  • Choi, Byung-Sun;Park, Seung-Soo;Kim, Yung-whan
    • Applied Chemistry for Engineering
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    • v.5 no.5
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    • pp.836-842
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    • 1994
  • The optimum design conditions of gas sparger pipe and the effects of operating variables on $SO_2$ removal efficiency have been examined in Jet Bubbling Reactor. Geometry of gas sparser pipe of Jet Bubbling Reactor is a very important factor to obtain a effective gas-liquid contact. Test results revealed that Reynolds numbers at sparger and slot have to be kept greater than 12,000 identically at a given gas velocity. $SO_2$ removal efficiency was a function of ${\Delta}P$, pH, inlet $SO_2$ concentration and particle size of limestone and was more sensitive to the change of ${\Delta}P$ than to the changes of others. The ${\Delta}P$ of at least 230mmAq must be maintained to acheive the above 90% $SO_2$ removal at pH of 4.0 which is considered as adequate operating pH. Higher $SO_2$ removal efficiency was obtained even at lower pH ranges, which resulted from the complete oxidation of the absorbed $SO_2$ to sulfates by adding air and consequently from the reduction of $SO_2$ equillibrium partial pressure in the gas-liquid interface The 99.5% of the limestone utilization was attained in pH range from 3.0 to 5.0 with regardless to the particle size of limestone employed.

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Development of 115K Tanker Design Adopted Ice Class 1A (Baltic Ice Class IA를 적용한 115K Ice Tanker 개발)

  • Kim, Hyun-Soo;Ha, Mun-Keun;Baek, Myeong-Chul;Kim, Soo-Young;Park, Jong-Woo;Chun, Ho-Hwan
    • Journal of the Society of Naval Architects of Korea
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    • v.41 no.6
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    • pp.120-125
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    • 2004
  • There are very few numbers of 115K FPP (Fixed Pitch Propulsion) Tankers for the Baltic ice class IA because the minimum power requirement of FMA (Finish- Swedish Maritime Association) needs quite large engine power and the 40 m Beam is out of calculation range of FMA minimum power requirements. The shipyard has no choice except to increase the engine power to satisfy FMA minimum power requirement Rule. And the operation cost, efficiency of hullform and its building cost are not good from the ship owners' point of view To solve this problem, the experience of ice breaking tanker development and the ice tank test results were adopted. The main idea to reduce the ice resistance is by reducing waterline angle at design load waterline. The reason behind the main idea is to reduce the ice-clearing force. Two hull forms were developed to satisfy Baltic Ice class IA. Two ice tank tests and one towing tank test was performed at MARC (Kvaener-Masa Arctic Research Center) and SSMB (Samsung Ship Model Basin) facilities, respectively. The purpose of these tests was to verify the performance in ice and open water respectively The hull form 2 shows less speed loss compared to Hull form 1 in open water operation but hull form 2 shows very good ice clearing ability. finally the Hull Form 2 satisfying Baltic ice class IA. The merit of this hull form is to use the same engine capacity and no major design changes in hull form and other related designs But the hull structure has to be changed according to the ice class grade. The difference in two hull form development methods, ice model test methods and analysis methods of ice model test will be described in this paper.

Study on the Analysis for the Effects of the Automated Speed Enforcement System Application (무인단속시스템 설치효과 분석에 관한 연구)

  • Joo, Doo-Hwan;Hyun, Cheol-Seung;Lee, Ho-Won;Han, Won-Sub;Lee, Choul-Ki
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.8 no.6
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    • pp.55-63
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    • 2009
  • Since the introduction of 32 Automated Speed Enforcement System in Korea 1997, their application was expanded to the entire nation and, as of January 2008, there are 3,212 Automated Speed Enforcement System that are in operation. The existing method of evaluating the effects of the Automated Speed Enforcement System application is simple comparison of a year before and a year after the application. If the number of traffic accidents dramatically increased(or decreased) the year before the application compared to the numbers from years before that, then if the number decreased(or increased) after the Automated Speed Enforcement System application, it cannot be concluded that the change was caused by the Automated Speed Enforcement System application alone. Based on the 4-Step Before-After study, the method of evaluating the effect of an Automated Speed Enforcement System application was analyzed anew. At 205 locations where Automated Speed Enforcement System was applied in 2006, the existing evaluation stated a reduction of 19% in the number of traffic accidents, but when reanalyzed for the method suggested in this paper, the effect of the Automated Speed Enforcement System was found to be $27.4{\pm}3$, along with an error range of ${\pm}12.89%$ due to miscellaneous improvement.

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Floating Point Converter Design Supporting Double/Single Precision of IEEE754 (IEEE754 단정도 배정도를 지원하는 부동 소수점 변환기 설계)

  • Park, Sang-Su;Kim, Hyun-Pil;Lee, Yong-Surk
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.48 no.10
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    • pp.72-81
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    • 2011
  • In this paper, we proposed and designed a novel floating point converter which supports single and double precisions of IEEE754 standard. The proposed convertor supports conversions between floating point number single/double precision and signed fixed point number(32bits/64bits) as well as conversions between signed integer(32bits/64bits) and floating point number single/double precision and conversions between floating point number single and double precisions. We defined a new internal format to convert various input types into one type so that overflow checking could be conducted easily according to range of output types. The internal format is similar to the extended format of floating point double precision defined in IEEE754 2008 standard. This standard specifies that minimum exponent bit-width of the extended format of floating point double precision is 15bits, but 11bits are enough to implement the proposed converting unit. Also, we optimized rounding stage of the convertor unit so that we could make it possible to operate rounding and represent correct negative numbers using an incrementer instead an adder. We designed single cycle data path and 5 cycles data path. After describing the HDL model for two data paths of the convertor, we synthesized them with TSMC 180nm technology library using Synopsys design compiler. Cell area of synthesis result occupies 12,886 gates(2 input NAND gate), and maximum operating frequency is 411MHz.

Peripheral Blood Lymphocytes as In Vitro Model to Evaluate Genomic Instability Caused by Low Dose Radiation

  • Tewari, Shikha;Khan, Kainat;Husain, Nuzhat;Rastogi, Madhup;Mishra, Surendra P;Srivastav, Anoop K
    • Asian Pacific Journal of Cancer Prevention
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    • v.17 no.4
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    • pp.1773-1777
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    • 2016
  • Diagnostic and therapeutic radiation fields are planned so as to reduce side-effects while maximising the dose to site but effects on healthy tissues are inevitable. Radiation causes strand breaks in DNA of exposed cells which can lead to chromosomal aberrations and cause malfunction and cell death. Several researchers have highlighted the damaging effects of high dose radiation but still there is a lacuna in identifying damage due to low dose radiation used for diagnostic purposes. Blood is an easy resource to study genotoxicity and to estimate the effects of radiation. The micronucleus assay and chromosomal aberration can indicate genetic damage and our present aim was to establish these with lymphocytes in an in vitro model to predict the immediate effects low dose radiation. Blood was collected from healthy individuals and divided into 6 groups with increasing radiation dose i.e., 0Gy, 0.10Gy, 0.25Gy, 0.50Gy, 1Gy and 2Gy. The samples were irradiated in duplicates using a LINAC in the radiation oncology department. Standard protocols were applied for chromosomal aberration and micronucleus assays. Metaphases were stained in Giemsa and 200 were scored per sample for the detection of dicentric or acentric forms. For micronuclei detection, 200 metaphases. Giemsa stained binucleate cells per sample were analysed for any abnormality. The micronuclei (MN) frequency was increased in cells exposed to the entire range of doses (0.1-2Gy) delivered. Controls showed minimal MN formation ($2.0%{\pm}0.05$) with triple MN ($5.6%{\pm}2.0$) frequency at the lowest dose. MN formation increased exponentially with the radiation dose thereafter with a maximum at 2Gy. Significantly elevated numbers of dicentric chromosomes were also observed, even at doses of 0.1-0.5Gy, compared to controls, and acentric chromosomes were apparent at 2Gy. In conclusion we can state that lymphocytes can be effectively used to study direct effect of low dose radiation.

Addition of Salts and Their Mixtures for Improvement of Storage Stability of Kimchi (김치의 저장성 향상을 위한 염혼합물의 첨가)

  • Kim, Woo-Jung;Kang, Kun-Og;Kyung, Kyu-Hang;Shin, Jae-Ik
    • Korean Journal of Food Science and Technology
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    • v.23 no.2
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    • pp.188-191
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    • 1991
  • A study was carried out to investigate the control effect of sodium and potassium phosphates, sodium citrate and three different salts mixtures on kimchi fermentation when they were added into half-fermented kimchi in the concentration range of $0.001{\sim}0.01\;M$. The salts mixtures added were sodium phosphates mixture(CA-A), addition of $NaNO_2$, Ca-EDTA and BHA to CA-A(CA-B) and substitution of BHA with sodium citrate in CA-B. The results showed that sodium phosphates and sodium citrate significantly inhibited the kimchi fermentation while potassium phosphate had little effect. The order of control effect was $Na_3PO_4-Na_2HPO_4-sodium\;citrate-NaH_2PO_4-K_2HPO_4-KH_2PO_4$. Among the salts mixtures, CA-A showed the most reducing effect in the fermentation rate followed by CA-C and CA-A. The mixture of CA-C could extend the time of holding pH $4.2{\sim}4.4$ by approximately 6 times at $4{\sim}25^{\circ}C$ when it was compared to control. The microbial growth study of total and Leuconostoc mesenteroides also showed a very significant decrease in their numbers.

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