• Economic and Environmental Geology
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• v.42 no.2
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• pp.121-131
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• 2009

The distribution characteristics of heavy metals for surface sediments in east oceanic dumping area (EDA) and west oceanic dumping area (WDA) are evaluated by grain sizes, minerals, sedimentation rates and compositions of heavy metals. The mean grain sizes in EDA and WDA range from $7.95{\Phi}$ to $8.51{\Phi}$ and $7.42{\Phi}$ to $8.15{\Phi}$, respectively. These are mostly belonging to the M (mud) type. Minerals in the surface sediments consist of illite with chlorite, smectite, and kaolinite. Sedimentation rates estimated by $^{210}Pb$ method in EDA and WDA are 1.11 mm/yr$\sim$1.73 mm/yr and 1.87 mm/yr, respectively. According to the interrelationship, concentrations of Ni, Cu, Cr, and Zn are closely associated with mean grain size, Al, and Fe, whereas concentrations of Cd and Pb are poorly associated with ones. The enrichment factors of these elements are higher than 1.5, suggesting that the concentrations of Cd and Pb in the surface sediments are affected by anthropogenic sources. The $I_{geo}$-class numbers of Cd and Pb in the surface sediments are mostly classified in 2 to 4, showing moderate to strongly polluted. These numbers in EDA are higher than that of WDA, and the highest number is 4, indicative of the strongly polluted class. Our results show that the disposed wastes at EDA include mineralogical wastes, dredged materials from sewage disposals, and sludges from constructions having materials of WDA. The annual amount of oceanic dumping in EDA is double than that in WDA.

• Journal of the Korean Institute of Landscape Architecture
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• v.46 no.4
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• pp.76-83
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• 2018

The purpose of this study is to analyze people who applied for and passed engineer landscape architecture examination that had been conducted from 2007 to 2016, the test frequency and trends by the question types in the landscape structure construction and management area, and the test tendencies and features by question types, and thereby to find the test trends for landscape structure construction and management in engineer landscape architecture examination. the analysis results are presented as follows: The people who applied for and passed engineer landscape architecture examinations that had been conducted from 2007 to 2016 were analyzed. as a result, the numbers of applicants and those who passed the examination have been on the decrease from 2011 and from 2012, respectively. the 10-year average rate of successful applicants for engineer landscape architecture examination was 11.2%. The test frequency and trends by the question types in the landscape structure construction area, and the test tendencies and features were analyzed. as a result, based on the key words in the seven categories (construction plan & process management, landscape materials, landscape planting foundation, work classification based construction, landscape estimation, basic structural mechanics, and survey), the questions about work classification based construction accounted for the largest, or 25.2%, and the questions about landscape planting foundation accounted for 3.3%. therefore, landscape planting foundation had lower test frequency and was less important than other categories. The test frequency and trends by the question types in the landscape management area, and the test tendencies and features were analyzed. as a result, based on the key words in the nine categories (operation and use & maintenance, pruning management, fertilization management, weed management, irrigation and drainage management, wintering management, pest management, and lawn management, and landscape facility management), the questions about operation and use & maintenance accounted for the largest, or 37.2%, and the numbers of the questions about fertilization management and irrigation & drainage management and of the questions about waterscape facility of landscape facility management have been on the increase from 2011 and from 2015, respectively. According to the analysis on the test tendencies for landscape structure construction and management areas in the examination there have been questions in a wide range and variety of categories. in terms of the landscape structure construction area, the frequency of questions in work classification based construction, landscape materials, and excellent quality in terms of the landscape management area, the frequency of questions in fertilization management, irrigation & drainage management, and waterscape facility of landscape facility management tends to increase because of environmental factors like climate change.

• Korean Journal of Agricultural and Forest Meteorology
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• v.12 no.1
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• pp.36-44
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• 2010

In order to rehabilitate forest sites damaged by wildfire via natural or artificial restoration, it is important to determine right tree species, which can acclimate to biogeoclimatic environment at the sites. The objectives of this study were to develop site index equation of different tree species for estimating forest productivity and to provide information on species selection for post-wildfire restoration. Site index equation was developed based on environmental information from wildfire damaged areas in Gangneung, Goseong, Donghae, and Samcheok, where were located in east coastal areas of South Korea. Despite the small numbers (4~5) of environmental variables used for the development of the site index equations, statistical analysis (e.g. mean difference, standard deviation of difference, and standard error of difference) showed relatively low bias and variation, suggesting that those equations can provide relatively high capability of estimation and practical applicability with high effectiveness. The small numbers of the variables enabled the model to be applied in a wide range of usages including determination of appropriate tree species for post-wildfire restoration. The estimation of forest site productivity showed the possibility of large distribution in east coastal region as the best site for Korean ash (Fraxinus rhynchophylla) and original oak (Quercus variabilis) that can be used for firebreak in the region. These results imply that damages by forest fire can be reduced significantly by replacing existing pure coniferous forests in the area with ones dominated by broad-leaved deciduous stands, which can play an important role as fire break and/or prevent a transition from surface fire to crown fire.

• Microbiology and Biotechnology Letters
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• v.33 no.2
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• pp.148-153
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• 2005

Malaria is a re-emerging infectious disease that is spreading to areas where it had been eradicated, such as Eastern Europe and Central Asia. To avoid the mortality from malaria, early detection of the parasite is a very important issue. The peripheral blood smear has been the gold standard method for the diagnosis of malaria infection. Recently, several other methods have been introduced for quantitative detection of malaria parasites. Real time PCR that employs fluorescent labels to enable the continuous monitoring of PCR product formation throughout the reaction has recently been used to detect several human malaria parasites. 18S rRNA sequences from malaria parasites have been amplified using Taqman real time PCR assay. Here, a SYBR Green-based real time quantitative PCR assay for the detection of malaria parasite-especially, Plasmodium vivax - was applied for the evaluation of 26 blood samples from Korean malaria patients. Even though SYBR Green-based real time PCR is easier and cheaper than Taqman-based assay, SYBR Green-based assay cannot be used because 18S rRNA cannot be specifically amplified using 1 primer set. Therefore, we used DBP gene sequences from Plasmodium vivax, which is specific for the SYBR Green based assays. We amplified the DBP gene from the 26 blood samples of malaria patients using SYBR Green based assay and obtained the copy numbers of DBP genes for each sample. Also, we selected optimal reference gene between ACTB and B2M using real time assay to get the stable genes regardless of Malaria titer. Using selected ACTB reference genes, we successfully converted the copy numbers from samples into titer, ${\sharp}$ of parasites per microliter. Using the resultant titer from DBP based SYBER Green assay with ACTB reference gene, we compared the results from our study with the titer from Taqman-based assay. We found that our results showed identical tendency with the results of 18S rRNA Taqman assay, especially in lower titer range. Thus, our DBP gene-utilized real time assay can detect Plasmodium vivax in Korean patient group semi-quantitatively and easily.

• Journal of Aquaculture
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• v.8 no.1
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• pp.47-58
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• 1995

Field studies on the seasonal succesion of phytoplankton population were carried out at the 25 stations of the breeding ground in Kogum-sudo, Southern coast of Korean peninsula in Feburuary, April, August and October, 1993. Sixty four species belonging to 40 genera were identified. Predominant species were mainly centric diatoms throughout the four seasons, two centric diatoms, Skeletonema costatum and Thalassiosira sp. and a pennate diatom, Thaiassionema nitzschioides in the winter; two pennate diatoms, Thaiassionema nitzschioides and Asterionella kariana, and especially a dinoflagellate, Heterocapsa triquetra (station 10) in the spring, two centric diatoms, S. costatum and Chaetoceros diadema in the summer; and a centric diatom, Rhizosolenia alata and a pennate diatom, Bacillaria paxillifer in the fall. The main red tide organisms in the breeding ground were dinoflagellates, Prorocentrum dentatum, P. minimum, P. triestinum, Ceratium furro, Gymnodinium sanguineum, Noctiluca scintillans, H. triquetra, Scrippsiella trichoidea and a diatom S. costatum in the Kogum Sudo. Seasonal phytoplankton cell numbers were in a wide range between $8.8\times10^3$ cells/l and 1.4\times10^6$ cells/l; The seasonal average cell numbers were $12.2\times10^4\pm5.9\times10^4$ cells/l $(mean\;\pm\;standard\; diviation)$ in the winter, $3.3\times10^4\pm1.4\times10^4$ cells/l in the spring, $48.4X10^4\pm40.0\pm10^4$ cells/l in the summer, and $3.6\times10^4\pm1.9\times10^4$ cells/l in the fall, respectively.

• Child Health Nursing Research
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• v.4 no.2
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• pp.177-192
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• 1998

The early postpartum period is crucial toward in recovery from childbirth and attainment of the maternal role. Maternal role attainment is a complex social and cognitive process of stimulus-response accomplished by learning. Helping for maternal role attainment is one of nursing goals in the early postpartum period. Based on King's conceptual framework for nursing, this study was planned as descriptive correlation study to determine the significant differences of the degree of nursery room nurses' role performance according to several variables of personal, interpersonal, and working system of nurses in nursery room. The purpose of this study was to contribute to the planning of nursing care to help maternal role attainment of the early postpartum period of mothers and to the development of relevant nursing theory. The data were collected from Feb. 3 to 28 by questionnaires with 273 nurses in nursery room. The instruments for this study were consisted of four parts : 21 questions for rot performance of nurse. 37 questions for personal system of nurse including 31 questions for role perception of nurse : 65 questions for interpersonal system including 63 questions for job stress of nurses , 18 questions for working system of nurse. The toos to measure role performance and role perception, and job stress of nurse were tested for internal reliability. Cronbach's Alphas were 0.9612, 0.9058, and 0.9649. The data were analysed by using in S.A.S. computerized program and included percentage, t-test, ANOVA Pearson Correlation Coefficient, and Duncan multiple range test. The conclusions obtained from this study are summerized as follows : 1. The mean score of the items of role performance was 2.12(SD=0.55) in Likert's 4 points scale. 2. The degree of role performance was significantly different according to role perception(p=0.0001), age (p=0.006), educational background(p=0.002) , and certificate of midwife (p=0.03) among variables of personal system of subjects. 3. The degree of role performance was significantly different according to job stress (p=0.0001) and numbers of children(p=0.006) among variables of interpersonal system of subjects. 4. The degree of role performance was significantly different according to having opportunities for baby(p=0.03), the degree of flexibility to bring baby to mother's room(p=0.046), the scope of visitor for baby(p=0.016) , the degree of flexibility of visiting for baby (p=0.049) , the degree of participation of nurse in establishing visiting rules(p=0.017), existence and/or nonexistance of rules for breast feeding(p=0.010) , existence and/or nonexistance of education for breast feeding (p=0.009), existence and/or nonexistance of breast feeding room(p=0.013) , concert methods for breast feeding (p=0.003), working place (p=0.0001), and career(p=0.019) among variables of personal system of subjects.

• Structural Engineering and Mechanics
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• v.90 no.6
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• pp.551-568
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• 2024

The present study deals with static and dynamic behaviors including forced vibrations of an elastic rectangular nano plate on the two-parameter foundation. Firstly, the rectangular plate is assumed to be subjected to uniformly distributed and eccentrically applied concentrated loads. The governing equations of the problem are derived by considering the dynamic response of the plate, employing a series of the Chebyshev polynomials for the displacement function and applying the Galerkin method. Then, effects of the non-essential boundary conditions of the plate, i.e., the boundary conditions related to the shearing forces, the bending moments and the corner forces, are included in the governing equation of motion to compensate for the non-satisfied boundary conditions and increase the accuracy of the Galerkin method. The approximate numerical solution is accomplished using an iterative process due to the non-linearity of the unilateral property of the two-parameter foundation. The plate under static concentrated load is investigated in detail numerically by considering a wide range of parameters of the plate and the foundation stiffnesses. Numerical treatment of the problem in the time domain is carried out by assuming a stepwise variation of the concentrated load and the linear acceleration procedure is employed in the solution of the system of governing differential equations derived from the equation of motion. Time variations of the contact region and those of the displacements of the plate are presented in the figures for various numbers of the two-parameter of the foundation, as well as the classical and nano parameters of the plate particularly focusing on the non-linearity of the problem due to the plate lift-off from the unilateral foundation. The effects of classical and nonlocal parameters and loading are investigated in detail. Definition of the separation between the plate and the two-parameter foundation is presented and applied to the given problem. The effect of the lift-off on the static and dynamic behavior of the rectangular plate is studied in detail by considering various loading conditions. The numerical study shows that the effect of nonlocal parameters on the behavior of the plate becomes significant, when nonlinearity becomes more profound, due to the lift-off of the plate. It is seen that the size effects are significant in static and dynamic analysis of nano-scaled rectangular plates and need to be included in the mechanical analyses. Furthermore, the corner displacement of the plate is affected more significantly from the lift-off, whereas it is less marked in the time variation of the middle displacement of the plate. Several numerical examples are presented to examine the sensibility of various parameters associated with nonlocal parameters of the plate and foundation. Both stiffening and softening nonlocal parameters behavior of the plate are identified in the numerical solutions which show that increasing the foundation stiffness decreases the extent of the contact region, whereas the stiffness of the shear layer increases the contact region and reduces the foundation settlement considerably.

• Journal of radiological science and technology
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• v.21 no.1
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• pp.35-39
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• 1998

We analyzed image factors to determine the characteristic factors that need for intelligent replenishment system of the auto film processor. We processed the serial 300 sheets of radiographic films of chest phantom without replenishment of developing and fixation replenisher. We took the digital data by using film digitizer which scaned the films and automatically summed up the pixel values of the films. We analyzed characteristic curves, average gradients and relative speeds of individual film using densitometer and step densitometry. We also evaluated the pH of developer, fixer, and washer fluid with digital pH meter. Fixer residual rate and washing effect were measured by densitometer using the reagent methods. There was no significant reduction of the digital density numbers of the serial films without replenishment of developer and fixer. The average gradients were gradually decreased by 0.02 and relative speeds were also gradually decreased by 6.96% relative to initial standard step-densitometric measurement. The pHs of developer and fixer were reflected the inactivation of each fluid. The fixer residual rates and washing effects after processing each 25 sheets of films were in the normal range. We suggest that the digital data are not reliable due to limitation of the hardware and software of the film digitizer. We conclude that average gradient and relative speed which mean the film's contrast and sensitivity respectively are reliable factors for determining the need for the replenishment of the auto film processor. We need more study of simpler equations and programming for more intelligent replenishment system of the auto film processor.

• Journal of Microbiology
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• v.45 no.2
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• pp.158-167
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• 2007

In this study, we have cloned a novel cDNA encoding for a papain-family cysteine protease from the Uni-ZAP XR cDNA library of the polychaete, Periserrula leucophryna. This gene was expressed in Escherichia coli using the T7 promoter system, and the protease was characterized after partial purification. First, the partial DNA fragment (498 bp) was amplified from the total RNA via RT-PCR using degenerated primers derived from the conserved region of cysteine protease. The full-length cDNA of cysteine protease (PLCP) was prepared via the screening of the Uni-ZAP XR cDNA library using the $^{32}P-labeled$ partial DNA fragment. As a result, the PLCP gene was determined to consist of a 2591 bp nucleotide sequence (CDS: 173-1024 bp) which encodes for a 283-amino acid polypeptide, which is itself composed of an 59-residue signal sequence, a 6-residue propeptide, a 218-residue mature protein, and a long 3'-noncoding region encompassing 1564 bp. The predicted molecular weights of the preproprotein and the mature protein were calculated as 31.8 kDa and 25 kDa, respectively. The results of sequence analysis and alignment revealed a significant degree of sequence similarity with other eukaryotic cysteine proteases, including the conserved catalytic triad of the $Cys^{90},\;His^{226},\;and\;Asn^{250}$ residues which characterize the C1 family of papain-like cysteine protease. The nucleotide and amino acid sequences of the novel gene were deposited into the GenBank database under the accession numbers, AY390282 and AAR27011, respectively. The results of Northern blot analysis revealed the 2.5 kb size of the transcript and ubiquitous expression throughout the entirety of the body, head, gut, and skin, which suggested that the PLCP may be grouped within the cathepsin F-like proteases. The region encoding for the mature form of the protease was then subcloned into the pT7-7 expression vector following PCR amplification using the designed primers, including the initiation and termination codons. The recombinant cysteine proteases were generated in a range of 6.3 % to 12.5 % of the total cell proteins in the E. coli BL21(DE3) strain for 8 transformants. The results of SDS-PAGE and Western blot analysis indicated that a cysteine protease of approximately 25 kDa (mature form) was generated. The optimal pH and temperature of the enzyme were determined to be approximately 9.5 and $35^{\circ}C$, respectively, thereby indicating that the cysteine protease is a member of the alkaline protease group. The evaluation of substrate specificity indicated that the purified protease was more active towards Arg-X or Lys-X and did not efficiently cleave the substrates with non-polar amino acids at the P1 site. The PLCP evidenced fibrinolytic activity on the plasminogen-free fibrin plate test.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.6
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• pp.2501-2504
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• 2014

Background: Capecitabine is an oral fluoropyrimidine derivative which is frequently used alone or in combination regimens for the treatment of metastatic breast cancer. Although overall and progression free survivals have increased in recent years with the use of new generation drugs, predictive factors that would further improve the outcomes are needed. Previous studies have demonstrated the relation between post-treatment increase in mean corpuscular volume (MCV) and predicting therapy response as well as survival. The present study investigated the clinical impact of MCV elevation in metastatic breast cancer patients treated with capecitabine. Materials and Methods: The data of a total of 82 patients from three centers followed between June 2005 and June 2013 were retrospectively analyzed. The demographic data and hormone receptor status of the patients, as well as initial examination before and after treatment and data concerning progression were recorded. MCV ${\geq}100$ fl was considered as macrocytosis. Capecitabine was given at a dose of $2500mg/m^2$ daily for 14 days every three weeks. Pre-treatment and post-treatment MCV and other parameters of complete blood count were recorded. Post-treatment initial evaluation was performed after 2 cycles of therapy. Results: The median age of the patients was 46.5 years (range 26-72 years) and 54% were premenopausal. Performance status was ECOG 0 and 1 in 81 (99%) patients. The median number of cycles for capecitabine therapy was 5 (min-max: 2-18). The median ${\Delta}MCV$ level (post-treatment values at sixth week - baseline) was 6.4. Whilst ${\Delta}MCV$ was ${\geq}6.4$ in 42 patients, it was <6.4 in 40 patients. Clinical benefit (complete response+partial response+stable disease) was observed in 37 (88%) of 42 patients with a median ${\Delta}MCV$ ${\geq}6.4$ and in 30 (75%) of 40 patients with ${\Delta}MCV$ <6.4 with no statistically significant difference (p=0.158). No significant difference was determined between the group with ${\Delta}MCV$ ${\geq}6.4$ and the group with ${\Delta}MCV$ <6.4 in terms of progression-free survival (11 vs 12 months) (p=0.55) and overall survival (20 months vs. 24 months) (p=0.11). Conclusions: The identification of new predictive markers in metastatic breast cancer is very important. In some recent studies, increase in MCV has been suggested as a marker in tumor response. In the present study, however, no significant difference was determined between tumor response and increase in MCV. Further studies including higher numbers of patients are needed to determine whether increase in MCV is a predictive marker or not.