• Korean Chemical Engineering Research
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• v.54 no.2
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• pp.223-228
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• 2016

In this study, we described a simple and facile method to generate uniform microwells poly(dimethyl siloxane) (PDMS) microstamps through micro-molding for efficient, rapid and reliable cell patterning of adipocyte differentiation. In contrast to the conventional methods, the microstamp technologies are low expensive, non-toxic, and using a small amount of solution. Recently, Orlistat known as tetrahydrolipstatin is a prescription drug designed to treat obesity which is used to aid in weight loss and help to reduce overweight obesity. Here, 3T3-L1 cells were treated under various concentration manners of Orlistat $0.2{\mu}M{\sim}5.0{\mu}M$. and it was confirmed maximum 26.5% inhibition activity compared to control. Thus, we elucidated this platform can be used for the real-time analyzing of cell proliferation, adipocyte differentiation for evaluation of anti-obesity agents on cell chip. Furthermore, we except that this platform technology designed here might be readily be expanded to discover a wider variety of anti-obesity agents.

• Polymer(Korea)
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• v.33 no.1
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• pp.52-57
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• 2009

This study investigates the metal adsorption properties of poly (lactic acid)-g-acrylic acid (PLA-g- AAc) synthesized by UV irradiation method. The properties including degree of grafting, water content, and ion exchange capacity (IC) strongly depend on the critical experimental factors such as UV intensity and AAc concentration. Under the optimized condition, the maximum degree of grafting, the IC value, and water content are 28%, 1.13 meq/g, and 38%, respectively. The synthesized ion exchanger shows strong capacity of adsorption for divalent metal ions such as $Cu^{2+}$, $Ni^{2+}$, and $Co^{2+}$, and greater selectivity of adsorption for $Cu^{2+}$.

• Journal of the Korean Society of Radiology
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• v.13 no.6
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• pp.847-856
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• 2019

The various environmental issues arose with the development of today's economy. naturally, people were increased interest in environment and the importance of research on drinking water and contamination are emerging especially. A number of country areas, uranium and ²²²Rn in ground water have been detected to people using as drinking water. So this study evaluated the way for more accurate measurements than when measuring ²²²Rn concentrations in the ground water containing. the experiment was performed using the low-level liquid scintillation counter with an alpha, beta analysis easy PSA function of pulse. the scintillator as the preparation of the ground water samples are mixed, the measure value detection is lowered over prepare period and expiration date. Energy spectrum was also moved to a lower side channel. As a long time to buy the scintillator and over time after opening, it was confirmed that detection is lowered. if the purpose is to use a different scintillator can see the energy through the channel change.

• Journal of Radiation Protection and Research
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• v.2 no.1
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• pp.9-16
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• 1977

A simple method has been established for determining RaA, RaB and RaC concentrations in airborne dust. This is to evaluate the concentration from measurement of total alpha activities in three selected-time intervals after an air sample is taken from the membrane filter paper (mean pore size: $0.8{\mu}m$). As a preliminary trial, a time-variation of the concentrations has been determined using the single-filter method at the KAERI site (N. Lat. $37^{\circ}38'$ and E. Long $127^{\circ}15'$), Seoul, Korea. It appears that there is a large variation of the concentrations depending on the sampling time. Generally the highest value was observed in the morning that may coincide with the highest density of atmosphere in a day while the lowest value was obtained around fourteen o'clock.

• Journal of Radiation Industry
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• v.8 no.2
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• pp.123-132
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• 2014

After the nuclear accident of the Fukushima Dai-ichi Nuclear Power Plants (FDNPPs) on 11 March 2011, a large amount of radioactive materials has been released into the atmosphere and the ocean. A compartment model is used to evaluate the circulation characteristics and the spatiotemporal concentration distributions of radionuclides in the ocean. In the comparison with observed concentrations of $^{137}Cs$ in seawater, calculated concentrations by the compartment model were well agreed with them. On the basis of these results, we performed evaluation of the effective dose and the cancer risk. In the early stage of the accident, the effective doses from ingestion of the seafood near the Fukushima region were much higher than 1 mSv which is the value of the annual effective dose limit to individual recommended by the International Commission on Radiological Protection (ICRP). However, the effective doses by ingestion of the seafood decreased below 1 mSv as distance from the FDNPPs increased and time passed. In addition, it was estimated that the cancer risks by intake of the contaminated marine products were less than natural occurrence probability of cancer. Consequently, it was inferred that the health risk due to the $^{137}Cs$ was low after since mid-term period of the accident.

• Journal of Radiation Industry
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• v.7 no.2_3
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• pp.191-200
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• 2013

In this study, a novel bombesin (BBN) analogues, DOTA-Ala($SO_3H$)-4 aminobenzoyl-Gln-Trp-Ala-Val-Gly-His-Leu-Met-$NH_2$ (DOTA-sBBN) and DOTA-Lys(glucose)-4 aminobenzoyl-Gln-Trp-Ala-Val-Gly-His-Leu-Met-$NH_2$ (DOTA-gluBBN), were synthesized and radiolabeled, and their binding affinities were evaluated. Peptides were prepared by a solid phase synthesis method and their purities were over 98%. DOTA is the chelating agent for $^{177}Lu$-labeling, and the DOTA-conjugated peptides were radiolabeled with $^{177}Lu$ by a high radiolabeling yield (>98%). The Log P values of DOTA-sBBN and DOTA-gluBBN were -2.20 and -2.79, respectively. 50.41% of $^{177}Lu$-DOTA-sBBN and 72.97% of $^{177}Lu$-DOTA-gluBBN were left undegraded by the serum incubation at $37^{\circ}C$ for 48 hr. A competitive displacement of $^{125}I-[Tyr^4]$-BBN on the PC-3 human prostate carcinoma cells revealed that 50% inhibitory concentration ($IC_{50}$) were 1.46 nM of DOTA-sBBN and 4.67 nM of DOTA-gluBBN indicating a highly nanomolar binding affinity for GRPR. Therefore, it is concluded that $^{177}Lu$-DOTA-sBBN and $^{177}Lu$-DOTA-gluBBN can be potential candidates as a targeting modality for the Gastrin-releasing peptide receptor (GRPR)-over-expressing tumors, and further studies to evaluate their biological and pharmacological characteristics are needed.

• Korean Journal of Microbiology
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• v.55 no.1
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• pp.17-24
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• 2019

Bacteria in the polar region are under strong light and ultraviolet radiation. In this study, we investigated the effects of light on the growth of a psychrophilic bacterium, Sphingomonas sp. PAMC 26621, isolated from an Arctic lichen Cetraria sp. The growth of the strain in the light was lower than that in the dark. Surprisingly, thiamine increased the growth of Sphingomonas sp. PAMC 26621 in M9 minimal medium under light conditions. Thiamine increased the growth of the strain in a concentration-dependent manner along with ascorbic acid. N-acetylcysteine had no effect on the growth of the strain in the light. Thiamine and ascorbic acid also increased the activities of glucose-6-phosphate dehydrogenase and superoxide dismutase. The results of this study indicate that thiamine provided by the lichen symbiosis system plays an important role in light-induced oxidative stress in this Arctic bacterium as an antioxidant. Our study provide insight into the biochemistry and physiology of Arctic bacteria under strong light and ultraviolet radiation.

• Current Optics and Photonics
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• v.6 no.6
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• pp.521-549
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• 2022

Fiber lasers have made remarkable progress over the past three decades, and they now serve far-reaching applications and have even become indispensable in many technology sectors. As there is an insatiable appetite for improved performance, whether relating to enhanced spatio-temporal stability, spectral and noise characteristics, or ever-higher power and brightness, thermal management in these systems becomes increasingly critical. Active convective cooling, such as through flowing water, while highly effective, has its own set of drawbacks and limitations. To overcome them, other synergistic approaches are being adopted that mitigate the sources of heating at their roots, including the quantum defect, concentration quenching, and impurity absorption. Here, these optical methods for thermal management are briefly reviewed and discussed. Their main philosophy is to carefully select both the lasing and pumping wavelengths to moderate, and sometimes reverse, the amount of heat that is generated inside the laser gain medium. First, the sources of heating in fiber lasers are discussed and placed in the context of modern fiber fabrication methods. Next, common methods to measure the temperature of active fibers during laser operation are outlined. Approaches to reduce the quantum defect, including tandem-pumped and short-wavelength lasers, are then reviewed. Finally, newer approaches that annihilate phonons and actually cool the fiber laser below ambient, including radiation-balanced and excitation-balanced fiber lasers, are examined. These solutions, and others yet undetermined, especially the latter, may prove to be a driving force behind a next generation of ultra-high-power and/or ultra-stable laser systems.

• The Korean Journal of Physiology and Pharmacology
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• v.25 no.1
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• pp.87-94
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• 2021

Skin photoaging occurs due to chronic exposure to solar ultraviolet radiation (UV), the main factor contributing to extrinsic skin aging. Clinical signs of photoaging include the formation of deep, coarse skin wrinkles and hyperpigmentation. Although melanogenesis and skin wrinkling occur in different skin cells and have different underlying mechanisms, their initiation involves intracellular calcium signaling via calcium ion channels. The ORAI1 channel initiates melanogenesis in melanocytes, and the TRPV1 channel initiates MMP-1 production in keratinocytes in response to UV stimulation. We aimed to develop a drug that may simultaneously inhibit ORAI1 and TRPV1 activity to help prevent photoaging. We synthesized nootkatol, a chemical derivative of valencene. TRPV1 and ORAI1 activities were measured using the whole-cell patch-clamp technique. Intracellular calcium concentration [Ca2+]i was measured using calcium-sensitive fluorescent dye (Fura-2 AM). UV-induced melanin formation and MMP-1 production were quantified in B16F10 melanoma cells and HaCaT cells, respectively. Our results indicate that nootkatol (90 μM) reduced TRPV1 current by 94% ± 2% at -60 mV and ORAI1 current by 97% ± 1% at -120 mV. Intracellular calcium signaling was significantly inhibited by nootkatol in response to ORAI1 activation in human primary melanocytes (51.6% ± 0.98% at 100 μM). Additionally, UV-induced melanin synthesis was reduced by 76.38% ± 5.90% in B16F10 melanoma cells, and UV-induced MMP-1 production was reduced by 59.33% ± 1.49% in HaCaT cells. In conclusion, nootkatol inhibits both TRPV1 and ORAI1 to prevent photoaging, and targeting ion channels may be a promising strategy for preventing photoaging.

• Journal of Radiation Protection and Research
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• v.46 no.4
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• pp.178-183
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• 2021

Background: This study was carried out to provide environmental transfer parameter values to estimate activity concentrations of these radionuclides in agricultural crops when direct contamination occurred. Materials and Methods: Mass interception fractions (F_Bs) and weathering half-lives (T_ws) of ¹³¹I and radiocesium were calculated using openly available monitoring data obtained after the Fukushima Daiichi Nuclear Power Plant accident. F_B is the ratio between the initial radioactivity concentration of a radionuclide retained by the edible part of the plant (Bq·kg^-1 fresh weight [FW]) and the amount of deposited radionuclide in that area (Bq·m^-2). T_w values can be calculated using activity concentrations of crops decreased with time after the initial contamination. Results and Discussion: Calculated F_B and T_w values for ¹³¹I and radiocesium were mostly obtained for leafy vegetables. The analytical results showed that there was no difference of F_Bs between ¹³¹I and radiocesium by t-test; geometric mean values for leafy vegetables cultivated under outdoor conditions were 0.058 and 0.12 m²·kg^-1 FW, respectively. Geometric mean T_w value of ¹³¹I in leafy vegetables grown under outdoor conditions was 8.6 days, and that of radiocesium was 6.6 days; there was no significant difference between T_w values of these radionuclides by Wilcoxon rank sum test. Conclusion: There was no difference between ¹³¹I and radiocesium for F_Bs and T_ws. By using these factors, we would be able to carry out a rough estimation of the activity concentrations of ¹³¹I and radiocesium in the edible part of leafy crops when a nuclear accident occurred.