• Korean Journal of Microbiology
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• v.39 no.3
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• pp.128-134
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• 2003

Transformation-associated recombination (TAR) cloning is based on co-penetration into yeast spheroplasts of genomic DNA along with TAR vector DNA that contains 5'- and 3'-sequences (hooks) specific for a gene of interest, followed by recombination between the vector and the human genomic DNA to establish a circular YAC. Typically, the frequency of recombinant insert capture is 0.01-1% for single-copy genes by TAR cloning. To further refine the TAR cloning technology, we determined the effect of GC content on target hooks required for gene isolation utilizing the $Tg\cdot\AC$ mouse transgene as the targeted region. For this purpose, a set of vectors containing a B1 repeated hook and Tg AC-specific hooks of variable GC content (from 18 to 45%) was constructed and checked for efficiency of transgene isolation by radial TAR cloning. Efficiency of cloning decreased approximately 2-fold when the TAR vector contained a hook with a GC content ～${\leq}23$% versus ～40%. Thus, the optimal GC content of hook sequences required for gene isolation by TAR is approximately 40%. We also analyzed how the distribution of high GC content (65%) within the hook affects gene capture, but no dramatic differences for gene capturing were observed.

• Journal of the Korean Society of Groundwater Environment
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• v.6 no.1
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• pp.42-47
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• 1999

This study considers pseudosteady-state flow to a restricted-entry well in a single or multilayer aquifer with crossflow. A simple method for calculating the pseudoskin factor caused by partial penetration is presented to overcome a limited applicability in geometrical or computational aspects of previous methods. The computation is based on the solution of a simplified pseudosteady-state equation that describes the long-time behavior of the closed radial system. We illustrate the applicability of this method to various types of cylindrical systems and provide the results graphically. Comparisons with previously published results have indicated that this method yields highly accurate estimates of pseu-doskin factor with minimum computational effort. This method has also shown to be particularly useful for geometrically-complicated systems. Greatly improved computational efficiency of pseudosteady-state approach permits the engineer to easily account for the effect of partial penetration on the late-time performance of a well.

• Composites Research
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• v.20 no.3
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• pp.1-7
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• 2007

Manufacturing process of the shear thickening fluid(STF) and evaluation of the ballistic penetration resistance performance of the Kevlar-STF composites were studied. STF was made from silica and ethylene glycol, and the Kevlar-STF composite was made by impregnating the STF into the Kevlar fabric. Specimens including neat Kevlar woven fabrics and Kevlar-STF composites with two types of silica were prepared and carried out the ballistic tests. From the results STFs represented shear thickening behavior irrespective of the silica type, and Kevlar-STF composite with spherical silica showed best ballistic penetration resistance performance among them. Especially the specimens of Kevlar-STF composites with spherical silica showed radial fiber deformation by the projectile during the tests, that was somewhat different deformation behavior from those of the neat Kevlar fabrics shown fiber pull-out phenomena or fracture.

• Geomechanics and Engineering
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• v.21 no.5
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• pp.489-501
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• 2020

This paper conducts a complicated coupled effective stress analysis of X-section-in-place concrete (XCC) pile installation and consolidation processes using the dual-stage Eulerian-Lagrangian (DSEL) technique incorporating the modified Cam-clay model. The numerical model is verified by centrifuge data and field test results. The main objective of this study is to investigate the shape effect of XCC pile cross-section on radial total stress, excess pore pressure and time-dependent strength. The discrepancies of the penetration mechanism and set-up effects on pile shaft resistance between the XCC pile and circular pile are discussed. Particular attention is placed on the time-dependent strength around the XCC pile shaft. The results show that soil strength improved more significantly close to the flat side compared with the concave side. Additionally, the computed ultimate shaft resistance of XCC pile incorporating set-up effects is 1.45 times that of the circular pile. The present findings are likely helpful in facilitating the incorporation of set-up effects into XCC pile design practices.

• Journal of Welding and Joining
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• v.23 no.1
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• pp.41-47
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• 2005

Arc pressure is one of important factors in understanding physical arc phenomena. Especially it affects on the penetration, size and shape of TIG welding. Some researches were reported on the effect of arc pressure in low and middle current region. But there are not any research in high current region. The purpose of this study is to investigate the arc pressure distribution with mixing ratio of shield gas such as Ar and He gases. A Cu block with water cooling was specifically designed and used as an anode electrode in order to measure the arc pressure in high current region. Then, the arc pressure distribution was measured with change in welding current and mixing ratio of shield gases. The arc force was obtained by numerically integrating the measured results. As the results, it was shown that the arc pressure was concentrated at the central part of the arc in middle and high current regions when a pure Ar gas was used. In case of Ar + He mixing gas, the arc pressure was much lower than that of pure Ar gas. In addition, it was widely distributed to radial direction.

• Journal of Mechanical Science and Technology
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• v.14 no.10
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• pp.1143-1150
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• 2000

The effects of ambient conditions on vaporizing sprays from a high-pressure swirl injector were investigated by an exciplex fluorescence method. Dopants used were 2% fluorobenzene and 9% DEMA (diethyl-methyl-amine) in 89% solution of hexane by volume. In order to examine the behavior of liquid and vapor phases inside of vaporizing sprays, ambient temperatures and pressures similar to engine atmospheres were set. It was found that the ambient pressure had a significant effect on the axial growth of spray, while ambient temperature had a great influence on the radial growth. The spatial distribution of vapor phase at temperatures above 473K became wider than that of liquid phase after half of injection duration. From the analysis of the area ratio for each phase, the middle part (region II) in the divided region was the region which liquid and vapor phases intersect. For liquid phase, fluorescence-intensity ratio was greatly changed at lms after the start of injection. However, the ratio of vapor phase was nearly uniform in each divided region throughout the injection.

• Advances in concrete construction
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• v.7 no.4
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• pp.203-210
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• 2019

Concrete structures in marine environment are susceptible to chloride attack, where chloride diffusion results in the corrosion of steel bar and further lead to the cracking of concrete cover. This process causes structural deterioration and affects the response of concrete structures to different forms of loading. This paper presents the use of ABAQUS Finite Element Software in simulating the processes involved in concrete's structural degradation from chloride diffusion to steel corrosion and concrete cover cracking. Fick's law was used for the chloride diffusion, while the mass loss from steel corrosion was obtained using Faraday's law. Pressure generated by steel corrosion product at the concrete-steel interface was modeled by applying uniform radial displacements, while concrete smeared cracking alongside the Extended Finite Element Method (XFEM) was used for concrete cover cracking simulation. Results show that, chloride concentration decreases with penetration depth, but increases with exposure time at the concrete-steel interface. Cracks initiate and propagate in the concrete cover as pressure caused by the steel corrosion product increases. Furthermore, the crack width increases with the exposure time on the surface of the concrete.

• Journal of ILASS-Korea
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• v.27 no.1
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• pp.1-10
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• 2022

In this study, an experimental investigation on the effects of the pressure ratio on the wall-impingement spray characteristics of nitrogen gas using a compressed natural gas (CNG) injector was conducted. The transient development of the impingement spray was recorded by a high speed camera with Z-type Schlieren visualization method. The spray behavior under various pressure ratio conditions were analyzed. The experimental results showed that the pressure ratio has positive effect on the development of spray wall-impingement. The effects of the above factor were evaluated in a constant volume chamber at atmospheric conditions. The data from test showed that, with the increase of the pressure ratio, the spray tip penetration (STP) quickly increases before the impingement and gradually increases after the impingement. Additionally, the spray velocity first increases and then sharply decreases on regardless of the injection pressure level. As the spray spreading angle increases, spray area and volume increases rapidly with the increase in STP at the beginning of injection, and finally entered a stable range, has a great correlation with the increase of pressure ratios.

• Journal of the Korean Society of Groundwater Environment
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• v.7 no.2
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• pp.89-96
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• 2000

Slug tests are the most widely used field method for quantification of hydraulic conductivity of porous media. Well recovery is affected by well casing, borehole radii, screened length, hydraulic conductivity, and specific storage of porous media. In this study, a new slug tests model was developed through finite element approximation and the validity and usefulness of the model were tested in various ways. Water level fluctuation in a well under slug test and cons-equent groundwater flow in the surrounding porous medium were appropriately coupled through estimation of well-flux using an iteration technique. Numerical accuracy of the model was verified using the Cooper et al. (1967) solution. The model has advantages in simulations for monitored slug tests, partial penetration, and inclusion of storage factor. Volume coverage of slug tests is significantly affected by storage factor. Magnitude and speed of propagation of head changes from a well increases as storage factor becomes low. It will be beneficial to use type curves of monitored head transients in the surrounding porous formation for estimation of specific storage. As the vertical component of groundwater flow is enhanced, the influence of storage factor on well recovery decreases. For a radial-vertical flow around a partially penetrated well, deviations between hydraulic estimates by various methods and data selection of recovery curve are negligible on practical purposes, whereas the deviations are somewhat significant for a radial flow.

• Transactions of the Korean Society of Automotive Engineers
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• v.10 no.5
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• pp.45-57
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• 2002

In this paper, the results gained by applying many impingement models to the cylinder and flat plate were analyzed in comparison with the experimental data to study a spray/wall interaction phenomena. To begin with, the behavior of spray injected normal to the wall was analysed using three different impingement models ; Naber and Reitz model(NR model), Watkins and Wang model(WW model) and Park and Watkins model(PW model) in the present calculation. The results obtained from these models were compared with experimental data of Katsura et. al. The results indicated that PW model was in better agreement with experimental data than the NR and WW model. Also f3r spray injected at 30DEG , the result of three models were compared with experimental data of Fujimoto et. al. The results showed that m model overpredicted the penetration in the radial direction because this model was based on the inviscid jet analogy. WW model did not predicted the radius and height of the wall spray effectively. It might be thought that this discrepancy was due to the lack of consideration of spray film velocity occurred at impingement site. The result of PW model agrees with the experimental data as time goes on. In particular, a height of the spray droplets was predicted more closely to the experimental data than the other two models. The results of PW model in which the spray droplets were distributed densely around the edge of droplet distribution shaped in a circle had an agreement with the experimental data of Fujimoto et. al. Therefore, it was concluded that PW model performed better than M and WW model for prediction of spray behavior. The numerical calculation using PW model performed to the cylinder similar to the real shape of DI engine. The results showed that vortex strength near the wall in the cylinder was stronger than that in the case of flat plate. Contrary to the flat plat, an existence of the side wall in the cylinder caused the tangential velocity component to be reduced and the normal velocity component to be increased. The flow tends to rotate to the inside of cylinder going upward to the right side wall of cylinder gradually as time passes. Also, the results showed that as the spray angle increases, the gas velocity distribution and the tumble flow seemed to be formed widely.