• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.73-73
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• 2018

Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1, K2, K3 and K3a. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 paired- end read was used for GBS sequencing. GWAS was conducted using T ASSEL 5.0. The T ASSEL program uses a mixed linear model (MLM). T he results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race, while the least number of accessions (34.37%) resisted K3a race. For races K2 and K3, the resistant germplasm proportion remained between 66.67 to 70.83%. T he genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than5 (K1 and K2) and more than4 (K3 and K3a) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. T hese SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races, whereas on chromosome 4, 6, 11, and 12 for K3 and K3a races. The significant loci detected have also been illustrated, NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

• Current Research on Agriculture and Life Sciences
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• 제32권3호
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• pp.111-117
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• 2014

고추 역병은 그 병원균의 토양전염성 때문에 약제에 의한 방제효과가 낮아 저항성 품종의 개발이 기대되어 왔다. 고추 역병에는 CM334, AC2258, PI201234 등 다수의 저항성 유전자원이 보고되었으며, 이들의 저항성의 유전에 관한 연구로 진행되었다. 그러나 저항성의 유전양식은 실험에 사용한 재료와 연구자에 따라 1개, 2개, 혹은 3개 이상의 유전자, 다수의 유전자에 의한 양적 유전 등 다양하였다. 최근에는 분자적 방법으로 양적형질유전자좌(QTL)를 구명하는 연구가 보고되고 있으며, 분자표지를 이용한 선발 기술도 이미 육종 현장에서 활용되고 있다. 최근 저항성 품종이 다수 출시됨에 따라 새로운 병원형(pathotype), 즉, 레이스(race)의 출현에 관심이 높아지면서 이에 대한 연구가 보고되고 있다. 모두 품종과 병원균주간에 특이적 변이가 있으며, 이를 토대로 몇 개의 병원형(race)으로 분류할 수 있었다. 그러나 판별품종이 통일되지 않았고 시험에 사용한 품종들의 저항성 유전자의 조성도 달라 세계적으로 통일된 레이스분류체계는 아직 없는 실정이다. 이러한 배경에서 보다 안정된 저항성 품종의 육성을 위해서는 한 가지 저항성 재료보다는 다수의 저항성 유전자원에서 저항성을 도입하고, 육성과정에 육성품종의 보급 대상지역의 여러 균주를 사용하여 선발하는 것이 필요할 것이다.

• Clinical and Experimental Pediatrics
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• 제48권11호
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• pp.1179-1186
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• 2005

목 적 : 재태 연령에 따른 신생아 출생체중의 정상치를 아는 것은 자궁내 발육부전과 과출생 체중아를 진단하여 의사들은 고위험 신생아를 조기에 진단하고 치료하여 이들의 유병률과 사망률을 줄일 수 있고, 의료정책 입안자들은 이들에 대한 적절한 의료서비스의 개발과 건강관리 프로그램을 개발하고 시행하는데 도움을 줄 수 있다. 이에 저자들은 우리나라의 재태 연령에 따른 출생체중의 기준치를 구하고자 본 연구를 시행하였다. 방 법 : 2001년 1월 1일부터 2003년 12월 31일까지 3년간 통계청의 인구동태자료 중 출생 자료에 기록되어 있는 1,552,375명 중 재태 연령이 24주에서 44주 사이의 단태아 1,509,763명을 대상으로 재태 연령에 따른 평균값과 표준편차를 구하고 10, 25, 50, 75, 90 백분위수를 구하였다. 또한 각 재태 연령별 분포곡선이 정규분포를 따르는지 알아보았다. 이중 정규분포를 따르지 않거나 쌍봉형을 나타내는 재태 연령에서 유한 혼합 모델을 이용하여 오류의 값을 제거하고 다시 평균과 표준편차 그리고 10, 25, 50, 75, 90 백분위수를 구하고 이에 따른 곡선을 그렸다. 결 과 : 원시자료를 통해 얻은 재태 연령에 따른 출생체중 곡선은 27주에서 32주 사이에 심한 혹이 나타난다. 이에 따라 재태 연령별 출생체중의 분포를 그렸을 때 24주에서 27주까지는 우측으로 긴 꼬리를 가지는 치우친 곡선을 보였고 28주에서 32주까지는 상봉형의 곡선을 보였다. 그리고 그 이후에는 거의 정규 분포를 따르는 곡선을 보였다. 이는 33주 미만에서 재태 연령의 기록에 오류가 있음을 나타내는 것으로 저자들은 유한 혼합 모델을 이용해서 재태 연령별 출생체중 분포를 분석한 후 오류의 부분을 제거 후 재태 연령에 따른 출생체중 곡선을 완성하였다. 이렇게 완성된 출생체중 곡선은 Lubchenco 등의 결과에 비해 10 백분위수에서 높은 값을 보였고 노르웨이나 북미의 연구 결과에 비해서는 전반적으로 낮은 값을 보였다. 결 론 : 본 연구에서 얻은 재태 연령에 의한 출생체중의 기준치와 곡선은 3년간 우리나라의 출생아 전수를 대상으로 오류를 객관적인 기준에 의해 제거하여 만든 것이다. 그러므로 우리나라를 대표하난 신생아의 재태 연령에 의한 출생체중의 기준 및 자궁내 발육부전이나 과출생 체중아의 진단의 기준으로 사용 할 수 있으리라 생각된다.

• 사상체질의학회지
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• 제32권1호
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• pp.39-49
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• 2020

The purpose of this study was to find out how sleep characteristics by Sasang constitution (SC) appear in Vietnamese immigrant women in Korea, and how sleep aberration by SC affected the quality of life. The number of 217 Vietnamese immigrant women joined this study. We surveyed SC with Korea Sasang Constitutional Diagnostic Questionnaire (KS-15) and sleep characteristics with Pittsburgh Sleep Quality Index(PSQI) questionnaire. We evaluated quality of life(QoL) with Euro Quality of Life-5Dimension(EQ 5D) questionnaire. A Chi-square test and a one-way ANOVA test were conducted according to SC, and T test also used to compare PSQI groups. Significant p was .05. The number(rate) of Taeeumin(TE), Soeumin(SE), and Soyangin(SY) were 40(18.4%), 55(25.3%), and 122(56.2%) respectively. The distribution of obstructive factors of sleep were significantly different according to SC(Too Cold in Sleeping, x =13.378, p=.037, Too Hot in Sleeping x²=12.834, p=.046). The distribution of daytime drowsiness(x²=15.379, p=.018) and falling concentration power (x²=17.533, p=.008) was significantly different according to SC. The sleep status of the subjects showed a significant negative correlation with the EQ 5D Visual Analogue Scale(VAS) (r =-.399, p<.001), and the EQ 5D Index (r=-.211, p=.002). In the EQ VAS, TE showed a difference between the good and bad sleeping groups (t=3.955, p<.001). SE showed a difference between the two groups in the EQ 5D Index (t=2.332, p=.037). SY did not show a difference between the two groups. This study reveals that sleeping is closely related with QoL and distribution of obstructive factors of sleep may be different according to SC. Further study to confirm the constitutional difference in race at Vietnam is needed.

• 복식문화연구
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• 제19권2호
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• pp.376-388
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• 2011

The purpose of this study is to examine the types and characteristics of Chinese indigo dyed designs performed until today in private houses of Chinese minority races and to examine the modern value of traditional dyeing crafts of minority races by investigating the cases of utilizing modern private Chinese indigo dyed designs. As the research methodology for theoretical study about the history of Chinese traditional pattern dyeing, the origin, types and characteristics of Chinese indigo dyed designs, the preceding research data, photo data, and Internet data were utilized, and for the empirical research on Chinese traditional dyeing techniques and the cases of utilizing today Chinese indigo dyed designs, the on-site survey on Guizhou and Yunnan was conducted during February and March 2008 and the data were collected and analyzed. Based on the results of this research, it was found that Chinese indigo dyed designs represented Chinese traditional pattern dyeing, which was inherited and developed mainly in Chinese minority races and Chinese. It could be seen that according to geographical distribution and characteristics of each race, the types and characteristics of dyeing techniques were formed, which are classified into the batik of the Miao of Guizhou in the Chinese southwestern region, the tie-dyeing of Bai of Yunnan, and Chinese blue calico(resist printing) of Chinese private houses in the Hunan and Huseo regions, and these represent the three typical Chinese dyeing techniques today. Based on the results of empirical research on the utilizing cases, focusing on the traditional living handicrafts of Chinese minority races, traditional cultural articles, and artworks, the modern values such as practical, cultural, and artistic values of Chinese indigo dyed designs could be rediscovered.

• Nutritional Sciences
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• 제8권2호
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• pp.111-117
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• 2005

It has been more than three decades since the first assay assessing circulating 25 (OH)D in human subjects was performed That publication as well as several that followed it defined 'normal' nutritional vitamin D status in human populations. Recently, the wisdom by which 'normal' circulating 25 (OH)D levels in human subjects were assigned in the past has come under question. It appears that sampling human subjects, who appear to be free from disease, and assessing 'normal' circulating 25 (OH)D levels by plotting a Gaussian distribution is grossly inaccurate. There are many reasons why this method is inaccurate, including race, lifestyle habits, sunscreen usage, age, latitude, and inappropriately low dietary recommendations for vitamin D. For instance, a 400 IU/day. AI for vitamin D is insignificant when one considers that a 10-15 minute whole body exposure to peak summer sun will generate and release up to 20,000 IU vitamin $D_3$ into the circulation. Recent studies, which orally administered up to 10,000 IU/day vitamin $D_3$ to human subjects for several months, have successfully elevated circulating 25 (OH)D levels to those observed in individuals from sun-rich environments. Further, we are now able to accurately assess sufficient circulating 25 (OH)D levels utilizing specific biomarkers instead of guessing what an adequate level is. These biomarkers include intact parathyroid hormone (PTH), calcium absorption, bone mineral density (BMD), insulin resistance and pancreatic beta cell function. Using the data from these biomarkers, vitamin D deficiency should be defined as circulating levels of 25 (OH)D$\leq$30 ng/mL. In certain cases, such as pregnancy and lactation, significantly higher circulating 25 (OH)D levels would almost certainly be beneficial to both the mother and recipient fetus/infant.

• Journal of Acupuncture Research
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• 제23권6호
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• pp.77-83
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• 2006

Objectives : The purpose of this study was to investigate the location of LI20 and ST3 by facial measurement according to Sasang Costitution, Sex and Age. Methods : To collect the photograph of face, we used the face measurement apparatus, and then analysed whether ST3 was located out of the nasolabial sulcus or not. Results : ST3 was located at the lateral to the nasolabial sulcus in 94.9%; 92.1% of Soyangin, 97.7% of Taeumin, 94.4% of Soeumin 92.3% of male, and 92.3% of female according to Sasang Constitution and sex. According to age distribution, the highest percentage(100%) was appeared at $16{\sim}20$ age, and the lowest(91.8%) was appeared at $70{\sim}74$ age. Conclusion : This study suggests that actual measurement would be nessesary with classical literature to determine the location of acupuncture point, and we need more study in other race.

• 한국작물학회지
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• 제35권2호
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• pp.171-175
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• 1990

1. Polyacrylamide gel electrophoresis를 이용하여 국내외 재래종과 야생종 대두 계통들의 trypsin inhibitor의 변이를 규명하기 위하여 본 시험이 시도되었으며 1706계통의 한국산 재래종과 103계통의 한국 야생종콩, 그리고 167계통의 외래 재배종과 71계통의 외국 야생종 대두가 공시되었다. 2. Trypsin inhibitor를 함유하지 않은 ti/ti형과 Ti c/c형은 한국 재래종에서만 발견되었으며, Ti$^{*}$ c형을 Hymowitz도 일본 대두품종에서 보고한 바 있으나 그도 이 계통은 한국 도래종일 가능성이 크다고 보고 한 바 있다. 3. 한국기원의 콩에서 trypsin inhibitor에 관한 이형접합형의 출현빈도가 외국 기원 콩 계통에서 보다 비교적 높았으며, 재래종에서 3.6%(N=61)와 야생종에서 9.7%(N=10)이었으며 종합적으로 보아 중국, 일본 등의 대두에 비해 한국 기원의 콩이 가장 큰 변이를 나타내고 있음을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제19권7호
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• pp.931-937
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• 2006

The full-length cDNA of the porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with both human and mouse. The promoter of SMPX was sequenced and then analyzed to find the promoter binding sites. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that SMPX has a high level of expression in heart and skeletal muscle, a very low expression in lung and spleen and no expression in liver, kidney, fat and brain. Moreover, SMPX has a differential expression level in skeletal muscle, the expression in 65-day embryos being higher than other stages. The porcine SMPX was mapped to SSCXp24 by using a somatic cell hybrid panel (SCHP) and was found closely linked to SW1903 using the radiation hybrid panel IMpRH. An A/G single nucleotide polymorphism (PCR-RFLP) in the 3'-untranslated region (3'-UTR) was detected in eight breeds. The analysis of allele frequency distribution showed that introduced pig breeds (Duroc and Large White) have a higher frequency of allele A while in the Chinese indigenous pig breeds (Qingping pig, Lantang pig, YushanBlack pig, Large Black-White pig, Small Meishan) have a higher frequencies of allele G. The association analysis using an experimental population (188 pigs), which included two cross-bred groups and three pure-blood groups, suggested that the SNP genotype was associated with intramuscular fat content.

• Asian-Australasian Journal of Animal Sciences
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• 제23권2호
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• pp.272-278
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• 2010

In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human $y^{+}$LAT1 and by using rapid amplification of cDNA ends (RACE), the full-length cDNA encoding porcine $y^{+}$LAT1 was isolated from porcine intestine RNA. It was 2,111 bp long, encoding a 511 amino acid trans-membrane glycoprotein composed of 12 transmembrane domains. The predicted amino acid sequence was found to be 91%, 90%, 87% and 87% identical to those of cattle, human, mouse and rat $y^{+}$LAT1 respectively. Real-time RT-PCR results indicated that the small intestine had the highest $y^{+}$LAT1 mRNA abundance and the lung had the lowest $y^{+}$LAT1 mRNA abundance. Baby hamster kidney (BHK) cells transfected with green fluorescent protein (GFP) tagged porcine $y^{+}$LAT1 cDNA indicated that the cellular localization of the gene product in BHK was on the plasma membrane.