• Title/Summary/Keyword: quinone reductase (QR)

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Potential Induction of Quinone Reductase Activity of Natural Products in Cultured Murine Hepa1c1c7 Cells

  • Heo, Yeon-Hoi;Lee, Sang-Kook
    • Natural Product Sciences
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    • v.7 no.2
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    • pp.38-44
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    • 2001
  • NAD(P)H:quinone reductase (QR), known as DT-diaphorase, is a kind of detoxifying phase II metabolic enzyme catalyzing hydroquinone formation by two electron reduction pathway from quinone type compounds, and thus facilitating excretion of quinoids from human body. With the usefulness of QR induction activity assay system for the modulation of toxicants, in the course of searching for cancer chemopreventive agents from natural products, the methanolic extracts of approximately two hundreds of oriental medicines were primarily evaluated using the induction potential of quinone reductase (QR) activity in cultured murine Hepa1c1c7 cells. As a result, several extracts including Hordeum vulgare, Momordica cochinchinensis, Strychnos ignatii, Houttuynia cordata, and Polygala japonica were found to significantly induce QR activity. In addition, the methylene chloride fraction of H. vulgare, one major dietary food source, showed potent induction of QR activity $(CD=6.4{\mu}g/ml)$. Further study for isolation of active principles from these lead extracts is warranted for the discovery of novel cancer chemopreventive agents.

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Screening of Quinone Reductase Inducers from Agricultural Byproducts Using Mouse Hepatoma Cell Line (Mouse hepatoma 세포를 이용한 농산부산물로부터 quinone reductase활성물질의 탐색)

  • Kim, Jong-Sang;Nam, Young-Jung;Kim, Joo-Won
    • Korean Journal of Food Science and Technology
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    • v.27 no.6
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    • pp.972-977
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    • 1995
  • The induction of phase II enzymes including quinone reductase [NAD(P)H dehydrogenase(quinone): NAD(P)H : (quinone acceptor) oxidoreductase, EC 1.6.99.2] is a major mechanism of whereby a large group of heterogeneous compounds prevent the toxic, mutagenic, and neoplastic effects of carcinogen. Using murine hepatoma cells(Hepalclc7 cells), quinone reductase(QR) inducers as the possible chemopreventive agents were screened from rice bran, wheat bran, soymilk residue, defatted soybean cake, defatted sesame and perilla residues. The 80% methanol extracts of defatted sesame and perilla residues induced quinone reductase significantly while the others did have little effect on the enzyme induction. Thin layer chromatography of the extracts showed that the fastest moving band(Rf=0.70) in the developing solvent of n-butanol : n-propanol : 2N ammonia(10 : 60 : 30) was responsible for the enzyme induction by the 80% methanol extracts of defatted sesame and perilla residues. Further identification of active component(s) is in progress.

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Induction of Quinone Reductase by Extracts of Traditional Medicinal Plants (Quinone reductase 유도활성 측정에 의한 약용식물의 항암활성 검색)

  • Jin, Hai-Hong;Kim, Dong-Man;Hyun, Chang-Kee
    • Korean Journal of Pharmacognosy
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    • v.32 no.2 s.125
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    • pp.168-174
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    • 2001
  • Induction of phase II enzymes is a major mechanism of chemoprevention. The induction levels of quinone reductase (QR) activity in cultured murine hepatoma (Hepa 1c1c7) cells by 80%-methanol extracts of traditional medicinal plants were measured. Among the tested 81 plants, the extracts of Aralia continentalis, Magnolia obovata, and Viscum album were found to induce QR activities over 250%. The maximum induction levels obtained were 401.9%, 270.5%, and 301.8% by treatments of the extracts of A. continentalis $(318\;{\mu}g/ml)$, M. obovata $(53.8\;{\mu}g/ml)$ and V. album $(80.6\;{\mu}g/ml)$, respectively. These QR induction activities were more potent than those of the known QR inducers, t-butylhydroquinone (170.1%) and ${\beta}-naphthoflavone$ (320.0%).

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Induction of Quinone Reductase Activity by Stilbene Analogs in Mouse Hepa 1c1c7 Cells

  • Heo, Yoen-Hoi;Kim, Sang-hee;Park, Jae-Eun;Jeong, Lak-Shin;Lee, Sang-Kook
    • Archives of Pharmacal Research
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    • v.24 no.6
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    • pp.597-600
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    • 2001
  • Based on the potential cancer chemoprebentive activity of resveratrol, a trihydroxystilbene with the induction of quinone reductase activeity this study was designed to determine if stilbene-related compounds were inducers of phase ll detoxifying metabolic enzyme quinone reductase (QR) in the mouse hepatoma Hepa 1c1c7 cells. Among the thirteen compounds tested, several compounds including 3,4,5,3',5'-pentamethoxy-trans-stibene were found to potentially induce QR activity in this cell line. In addition, substitution with 3-thiofurane ring instead of phenyl ring in the stilbene skeleton also exhibited potential induction of QR activity. This result will give primary information to design the potential inducers of QR activity in the stilbene analogs.

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Effect of Sophorae Flos Aqua-acupuncture Solution on the Quinone Reductase and Glutathione S-transferase Activities of Hepa 1c1c7 Cells (괴화약침액이 간세포의 Quinone redutase 와 Glutathione S-transferase 활성에 미치는 영향)

  • Lee Ki-Taek;Lim Jong-Kook
    • Korean Journal of Acupuncture
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    • v.20 no.1
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    • pp.39-43
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    • 2003
  • Sophorae Flos aqua-acupuncture solution(SFAS) was prepared and tested for the induction of quinone reductase and glutathione S-transferase activities and glutathione. SFAS significantly induced QR activity at the concentrations of $0.5{\times},\;1{\times}\;and\;3{\times}$ in cell culture. However, GST activity in murine Hepa 1c1c7 cells was slightly increased with SFAS. SFAS increased GSH levels.

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Growth Inhibitory and Quinone Reductase Activity Stimulating Effects of Internal Organs of Todarodes pacificus Fractions on Human Cancer Cell Lines In vitro (오징어내장 분획물의 in vitro에서의 암세포 성장억제 및 quinone reductase유도 활성 증가 효과)

  • Shin, Mi-Ok;Bae, Song-Ja
    • Journal of Life Science
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    • v.19 no.9
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    • pp.1251-1257
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    • 2009
  • We investigated the growth inhibitory effect of internal organs of Todarodes pacificus (TP) on proliferation in human cancer cell lines in vitro. The internal organs of TP were extracted with methanol (TPM), which was then further fractionated into four subfractions by using a solvent partition method, resulting in hexane (TPMH), methanol (TPMM), butanol (TPMB), and aqueous (TPMA) soluble fractions. We determined the cytotoxic effect of these four fractions in three kinds of cancer cell lines - HepG2, MCF-7 and HT-29 - by MTT assay. Among the four subfractions of TPM, TPMH showed the strongest cytotoxic effects at a concentration of $300{\mu}g$/ml, displaying 91.56% on the HepG2 cell line and 85.93% on the MCF-7 cell line. Morphological changes such as membrane shirinking and blebbing of cells were also observed during TPMH treatment of HepG2 cells. In addition, we also observed quinone reductase (QR) induced effect in the methanol (TPMM) layer of HepG2 cells. TPMM showed the highest induction activity of quinone reductase on HepG2 cells among the other partition layers. The QR induced effect of TPMM was determined to be 2.7 at a level of $360{\mu}g$/ml with a control value of 1.0. Although further studies are needed, the present work suggests that internal organs of Todarodes pacificus (TP) may be a chemopreventive agent for the treatment of human cells.

Growth Inhibitory and Quinone Reductase Activity Stimulating Effects of Internal Organs of Aplysia kurodai Fractions on Cancer Cell Lines In vitro (군소내장 분획물의 in vitro에서의 암세포 성장억제 및 quinone reductase 유도 활성 증가 효과)

  • Shin, Mi-Ok
    • Journal of Life Science
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    • v.20 no.6
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    • pp.877-884
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    • 2010
  • We investigated the growth inhibitory effect of internal organs of Aplysia kurodai (AK) on proliferation in cancer cell lines in vitro. The internal organs of AK were extracted with methanol (AKM), which were then further fractionated into four subfractions by using solvent partition method, resulting in hexane (AKMH), methanol (AKMM), butanol (AKMB), and aqueous (AKMA) soluble fractions. We determined the cytotoxic effect of these four fractions in four kinds of cancer cell lines - HepG2, MCF-7, HT29 and B16-F10 - by MTT assay. Among the four subfractions of AKM, AKMM showed the strongest cytotoxic effects on all cancer cell lines which were used. Morphological changes such as membrane shrinking and blebbing of cells were also observed in AKMM treatment in HepG2 cells. In addition, we also observed quinone reductase (QR) induced effect in the methanol layer (AKMM) of HepG2 cells. AKMM showed the highest induction activity of quinone reductase on HepG2 cells among the partition layers. The QR induced effect of AKMM was determined to be 2.4 at $100\;{\mu}g/ml$ level with a control value of 1.0. Although further studies are needed, the present work suggests that internal organs of Aplysia kurodai (AK) may be a chemopreventive agent for the treatment of human cells.

Taraxacum mongolieum Hand-Mass Aqua-acupuncture Solution as the Blocking Agent of Carcinogenesis (포공영약침액의 발암과정 blocking agent로서의 활성)

  • 손윤희;김소연;임종국;남경수
    • Journal of Life Science
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    • v.12 no.5
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    • pp.549-554
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    • 2002
  • Taraxacunf mongofieum Hand-Mass aqua-acupuncture solution (TMAS) was prepared and investigated og, the effect on initiation of carcinogenesis. The following effe.Is as a blocking agent were measured. .(a) Indu.ction of quinone reductase, (b) Induction of glutathione S-transferase activity (c) Increase of reduced glutathione. TMAS was potent inducer of quinone reductase in Hepa Iclc7 murine hepatoma cells. Clutathione S-transferase activity was increased with TMAS. In addition glutathione levels were increased about 1.6-fold with TMAS in cultured murine hepatoma Hepa Iclc7 cells.

The Effects on Quinone ReductaseInduction of Daucus carota L. (당근 추출 성분의 Quinone reductase 유도활성 효과)

  • 한은주;노승배;배송자
    • Journal of Life Science
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    • v.10 no.1
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    • pp.79-85
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    • 2000
  • Chemoprevention is one of the major strategies for cancer control. It is well established that dietary factors play an important role in modulating the development of certain types of human cancer. The experiment was conducted to determine quinone reductase(QR) activity induction of Daucus carota L. on HepG2 cells. Among various partition layers of roots of Daucus carota L., the ethyl acetate partition layer(DCMEA) and the n-hexane partition layer(DCMH) tested to be most effective which resulted 2.1 and 1.6 respectively compared to the control value of 1.0. In the case of seeds of Daucus carota L. n-butanol partition layer (DCMB) on HepG2 cells at a dose of 200 $\mu\textrm{g}$/$m\ell$ showed the highest induction activity of QR which was 3.0. These results suggest that potentially useful cancer chemoprevention chemicals could be isolated from DCMEA and DCMH of the roots and DCMB of the seeds of Daucus carota L.

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Induction of Quinone Reductase , an Anticarcinogenic Marker Enzyme, by Vitamin E in Both Hepalclc7 Cells and Mice

  • Kwon, Chong-Suk;Kim, Jong-Sang
    • Preventive Nutrition and Food Science
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    • v.4 no.2
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    • pp.122-124
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    • 1999
  • Induction of NAD(P)H : (quinone-acceptor) oxidoreductase (QR) which obligatory two electron reduction of quinones and prevents their participation in oxidative cycling and thereby the depletion of intracellular glutathione, has been used as a marker for chemopreventive agents. We postulated that vitamin E, an antioxidant, which induces QR as the gene of QR was reported to contain antioxidant reponsive element in the 5'-flanking region. Vitamin E resulted in significant induction of QR in both hepalclc7 cells and mouse tissues. QR induction was observed; to be maximal at 25uM vitamin E for hepalclc7 cells while it was maximal in the level of 2.5∼5 μmoles vitamin E/㎏ BW for mouse tissues. Thus the cancer-preventive effect of vitamin E may be exerted by it induction of intracellular detoxifying enzymes.

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