• 한국동물위생학회지
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• 제41권2호
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• pp.79-83
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• 2018

This study compared serum concentrations of suppression of tumorigenicity 2 (ST2) and N-terminal pro-B-type natriuretic peptide (NT-proBNP) between healthy and heartworm- infected dogs. Eighteen heartworm-infected dogs and five healthy dogs were included in the study. Dogs were diagnosed and categorized by history, clinical signs, and blood assay, thoracic radiography, echocardiography, and commercial ELISA kit results. Serum samples were sent to the IDEXX reference laboratory for NT-proBNP measurement. ST2 was examined by using a canine interleukin 33 receptor ELISA kit with the quantitative sandwich ELISA method. The severely infected group showed significant elevation of NT-proBNP concentration over those of the control (P=0.03) and mildly infected (P=0.04) group. There were no significant difference in ST2 concentrations among the three groups. The usefulness of NT-proBNP as a cardiac biomarker in dogs with severe heartworm disease was confirmed by the results of this study. Further investigations to assess ST2 as a cardiac biomarker are warranted.

• 대한토목학회논문집
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• 제37권3호
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• pp.629-636
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• 2017

도시철도는 수도권 및 주요 광역지자체에서 버스와 함께 대표적으로 수도권 시민이 가장 많이 이용하는 교통수단이다. 그러나 관련 연구 결과를 살펴보면 안내표지에 대한 만족도는 낮고, 안내표지 개선의 경우 대부분 안내표지의 설치위치에 대해 안내표지가 많이 또는 적게 설치되어 있다는 것을 사진 분석 위주로 제시하고 있어 정량적인 분석 기법을 통한 문제점 도출 및 개선이 어려웠다. 따라서 본 연구에서는 Test-bed 역사(수서역)을 대상으로 환승 동선(분당선 ${\rightarrow}$ 3호선 방면)내 주요의사결정지점에서의 안내표지 정보부하 및 적정 설치위치를 Positive Guidance를 적용하여 평가하는 방안을 제시하였다. Positive Guidance는 운전자 측면에서의 제공되어야 하는 적정 정보량과 정보 제공 위치 선정을 평가하는 방법으로 FHWA에 의해 1973년에 처음으로 도입되었다. 해당 기법을 통한 평가 결과 해당 동선 내 주요 의사결정지점에서 동선 결정을 방해하는 요인(다수의 시인성 저하 시설물, 높은 안내표지 정보 부하량)이 많아 이용자들의 혼란이 발생하고 있었다. 따라서 환승 동선 내 결절점 중심으로 필요 정보를 제공해야 하며, 시거 방해 요인(높은 조도의 광고 표지, 원형기둥, 높은 천장 등)을 제거하기 위한 안내표지 설치 방안이 필요함을 도출하였다. 향후 안내표지 디자인 개선시 유니버설 디자인을 적용하여 모든 이용자들이 도시철도역사 이용시 불편함이 없도록 해야 할 것이며, 해당 연구는 향후 도시철도역사 안내표지의 적정성을 평가하는 정량적인 방법으로 안내표지 유형 및 배치에 중요한 기초자료로 활용 가능할 것이다.

• 한국습지학회지
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• 제25권4호
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• pp.408-416
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• 2023

IUCN 생태계 적색목록은 생물다양성의 감소 위험이 높은 생태계를 평가하고 식별하기 위한 글로벌 표준으로, 효과적인 생태계 관리 및 보전 정책 수립에 필요한 과학적 근거를 제공한다. IUCN 생태계 적색목록은 쿤밍-몬트리올 글로벌 생물다양성 프레임워크 목표 A의 핵심지표(A.1)로 지정하였다. 생태계 적색목록 평가는 특정 생태계 유형을 분포의 감소(기준 A), 제한된 분포(기준 B), 환경 황폐화(기준 C), 생물학적 상호작용의 변화(기준 D), 생태계 붕괴 위험의 정량적 추정(기준 E)으로 생태계 붕괴 징후를 판별한다. IUCN 생태계 적색목록은 2014년부터 110개 이상의 국가에서 평가를 진행하였다. 80% 이상이 육상 및 육상-담수 생태계에서 평가를 진행하였으며, 이중 열대림과 아열대림에 위협 받는 생태계가 집중 분포하고 있다. 평가 기준은 공간적 징후(기준 A 및 B)에서 68.8%로 집중되어 있다. 국내에서 생태계 적색목록을 평가를 적용하기 위한 고려사항은 크게 세가지로 나뉜다. 첫째, 국내 적용가 능한 지구 생태계 유형(GET)을 적립해야 한다. 둘째, 생태계 적색목록 범주·기준 중 공간적 징후 평가에서 국내의 소규모로 이루어진 다양한 생태계 유형에 적용 가능한지를 판단해야 한다. 마지막으로 평가를 위한 활용 가능한 시계열(50년) 데이터 수집을 고려해야 한다. 이러한 고려사항을 바탕으로 국내에 IUCN 생태계 적색목록 평가를 적용하면, 우리나라의 독특한 생태계 유형의 현재 상태를 정확하게 파악할 수 있을 것이며, 이는 세계적인 생태계 보전 및 복원 노력에 기여할 것이다.

• 한국동물위생학회지
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• 제34권4호
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• pp.341-352
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• 2011

Colibacillosis in pigs remain a major swine industry bruden worldwide. Although some progress has been made in treating collibacillosis in pigs by using biosecurity and antimicrobials, it still remain a considerable problem. The use of host-specific bateriophages as a biocontrol is one possible alternative. The purpose of this study was to evaluate the effect of bacteriophage against enterotoxigenic Escherichia coli (ETEC) K88 infection in piglets. Twenty-eight piglets were randomly divided into four groups and each group was allocated with 7 pigs. Group B, C and D were inoculated with 5 ml of ETEC K88 ($1{\times}10^8$ CFU/ml) per head of piglet via oral. Group C and D were fed with bacteriophages (Group C, $1.0{\times}10^6$ PFU/g; Group D, $1.0{\times}10^8$ PFU/g; CJ CheilJedang Corp., Korea) orally as treatment. In piglets administrated bacteriophages and challenged with ETEC K88 (Group C and D), Clinical signs and the growth performance were improved and antibody titers were maintained low level compared with piglets challenged with ETEC K88 (Group B, P<0.05). Group B were shown high pH in the alimentary tracts compared with other piglets (P<0.05). In quantitative analysis by real-time PCR, the results of Group C and D were lower than those Group B in faecal and intestinal samples (P<0.05). Severe villus atrophy and crypt hyperplasia were observed in Group B consequently V/C ratio increased, compared with other piglets. These results indicate that feeding with bacteriophage has effect to prevent ETEC K88 infection in piglets and suggest that use of bacteriophage can be considered a valid antibiotic alternative.

• 한국건설관리학회논문집
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• 제20권1호
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• pp.62-72
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• 2019

북한 건설 인프라 재건사업은 남 북한 경제협력 초기 단계에서 급격한 수요증가가 발생될 것으로 예상되고 있다. 그러나 현재까지 북한 건설 인프라에 대한 현황 분석은 과거 시점을 기준으로 이루어져있을 뿐만 아니라, 정성적인 접근을 통해 장황하게 서술되어 있는 경우가 대다수이다. 북한 내 건설 인프라 시설물별 구축 현황 및 노후화 진행 정도 등에 대한 일관된 평가 체계가 부재함에 따라 인프라 현황에 대해 신속한 적정 수준 판단 및 향후 재건사업의 범위 및 규모 등에 대한 선제적인 의사결정 수립이 어려운 실정이다. 이에 본 연구에서는 우선 북한 건설 인프라의 기능 및 노후화 정도 등과 관련된 구축 현황 정보와 운영 실태를 파악하기 위한 평가 요소를 체계화하고, 인프라의 적정 수준 평가에 활용될 수 있는 성능지표를 제시하고자 한다. 그리고 둘째, 평가지표를 기반으로 북한 개별 인프라에 대한 구축 현황을 신속하게 판단할 수 있는 적용 방안을 리포트카드 형태로 마련하였다. 본 연구의 결과인 성능지표 및 리포트카드 기틀은 향후 북한 건설 인프라의 구축 현황 및 향후 투자범위 등에 대한 의사결정에 기반을 제공할 것으로 기대된다.

• 한국동물위생학회지
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• 제46권4호
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• pp.269-281
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• 2023

In this study, a new triplex real-time quantitative reverse transcription polymerase chain reaction (tqRT-PCR) assay was developed for the rapid and differential detection of three feline viral pathogens including feline calicivirus (FCV), feline herpesvirus 1 (FHV-1), and influenza A virus (IAV) in a single reaction. The assay specifically amplified three targeted viral genes with a detection limit of below 10 copies/reaction. The assay showed high repeatability and reproducibility, with intra- and inter-assay coefficients of variation of less than 1%. Based on the diagnostic results of the assay using 120 clinical samples obtained from cats with feline respiratory disease complex (FRDC)-suspected signs, the prevalence of FCV, FHV-1, or IAV was 43.3%, 22.5%, or 0%, respectively, indicating that the diagnostic sensitivity was comparable or superior to those of previously reported monoplex qRT-PCR/qPCR assays. The dual infection rate for FCV and FHV-1 was 8.3%. These results indicate that FCV and FHV-1 are widespread and that co-infection with FCV and FHV-1 frequently occur in the Korean cat population. The developed tqRT-PCR assay will serve as a promising tool for etiological and epidemiological studies of these three bacterial pathogens, and the prevalence data for three feline viruses obtained in this study will contribute to expanding knowledge about the epidemiology of FRDC in the current Korean cat population.

• 대한지리학회지
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• 제47권4호
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• pp.541-553
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• 2012

한국도시지리학의 태동기 ${\rightarrow}$ 성립기 ${\rightarrow}$ 전환기 ${\rightarrow}$ 절정기로 구분될 수 있으며, 이러한 추세가 지속된다면 향후 성숙기의 단계로 접어들 것으로 전망된다. 본 연구는 한국도시지리학의 연구동향을 회고하기 위해 1960~2012년의 62년간 출간된 논문 686편과 단행본 177권을 연구영역별 연구지역별 분석방법별 및 학회지별로 고찰한 것이다. 도시지리학자의 수적 증가에 따라 학자들 간에 상호관계가 더 복잡하고 다양하게 나타나고 있으나 학파의 징후는 아직 보이지 않고 있다. 도시연구지역은 여전히 수도권을 대상으로 한 논문이 많고, 해외의 경우는 미국도시로부터 벗어나 다양화되어가고 있음이 밝혀졌다. 도시연구방법은 계량화가 더 많아지는 경향이 있고, 2000년 이후부터는 한국도시지리학회에 게재된 논문이 국내 학회지 중 가장 많았다.

• Journal of Oral Medicine and Pain
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• 제24권4호
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• pp.455-466
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• 1999

The purpose of this study was to investigate the magnitude of mandibular rotational torque movements in subjects with TMJ sounds, and to analyse correlation between quantitative characteristics of TMJ sounds and mandibular rotational torque movement. Twenty dental college students with TMJ clicking and twenty students without any TMD signs and symptoms were examined by mean of SonoPak and Rotate program of BioPAK system(Bioresearch Inc. MilWaukee, wisconsin, USA) in this study. Mandibular rotational torque movements were recorded and analysed during maximum mouth opening, protrusion, and lateral excursion in frontal and horizontal planes. The obtained results were as follows: 1. On maximum mouth opening, mandibular rotational angle and distance of clicking group were significantly greater than those of control group in frontal plane. (P<0.05). 2. During maximum mouth opening closing, maximum mandibular rotational angle and distance of clicking group were significantly greater than those of control group in frontal plane. (P<0.01). 3. On protrusion, mandibular rotational angle and distance of clickin group were significantly greater than those of control group in horizontal plane. (P<0.05). 4. On lateral excursion, there was no significant difference in mandibular rotational angle and distance between clicking group and control group in frontal and horizontal planes. 5. There were significant correlations between peak amplitude of TMJ sounds and maximum mandibular rotational distance during maximum mouth opening (r=-.481) and mandibular rotational distance on maximum mouth opening (r=-.455) in horizontal plane. 6. There were significant correlations between Above 300/(0-300)Hz ratio of TMJ sounds and mandibular rotational angle (r=-.499) and distance (r=-.457) on maximum mouth opening in frontal plane.

• Journal of Oral Medicine and Pain
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• 제45권3호
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• pp.56-64
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• 2020

Purpose: Temporomandibular disorders (TMD) is a mosaic of clinical signs and symptoms that can be regarded as a set of phenotypes that are affected by various factors including pain sensitivity, pain disability, sleep and psychological functioning. The aims of this study were to evaluate association of pain experience, sleep quality and psychological distress with different phenotypes of TMD patients. Methods: This retrospective study included a cohort (n=1,858; 63.8% for female, mean age=34.9±15.9 years) of patients with TMD. A set of self-administered questionnaires concerning pain interference (Brief Pain Inventory), pain disability (Graded Chronic Pain Scale), sleep quality (Pittsburg Sleep Questionnaire Index), psychological distress (Symptom Checklist-90 revised), and pain catastrophizing (Pain Catastrophizing Scale) were administered to all participants at the first consultation. All TMD patients were classified into four groups including TMD with internal derangement without pain (TMD_ID, n=370), TMD with joint pain (TMD_J, n=571), TMD with muscle pain (TMD_M, n=541) and TMD with muscle-joint combined pain (TMD_MJ, n=376). Results: The female ratio was particularly high in the group with TMD_MJ (p=0.001). The patients with muscle pain and both muscle and joint pain had longer symptom duration (p=0.004) and presented significantly higher scores in pain experience (p<0.001), subjective sleep quality (p<0.001), pain catastrophizing (p<0.001) and psychological distress (p<0.05) except for paranoid-ideation than the groups with only joint problems. Conclusions: The results of this study highlight the importance of multi-dimensional approach that consider pain disability, sleep quality, and psychological functioning in the management of TMD with muscle component. This study would contribute to a better understanding of interaction between heterogeneous TMD and multiple risk factors in order to build tailored treatment based on different phenotypes.

• 한국어병학회지
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• 제24권3호
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• pp.279-287
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• 2011

Transcriptional response patterns of mud loach (Misgurnus mizolepis; Cypriniformes) hepcidin, a potential ortholog to human hamp1, in response to experimental challenges with non-pathogenic and pathogenic bacterial species were analyzed based on the semi-quantitative reverse transcription-PCR assay. Mud loach hepcidin transcripts were much more preferentially induced by pathogenic bacterial species (Edwardsiella tarda and Vibrio anguillarum) causing apparent pathological symptoms than by non-pathogenic species (Escherichia coli and Bacillus thuringiensis) displaying neither clinical signs nor mortality. However in overall, the induced amounts of hepcidin transcripts were positively related with the number of bacterial cells delivered in both pathogenic and non-pathogenic bacterial species. Inducibility of hepcidin transcripts were variable among three tissues examined (liver, kidney and spleen) in which kidney and spleen were more responsive to the bacterial challenge than liver. Time course expression patterns of hepcidin mRNAs after challenge were different between groups challenged with pathogenic and non-pathogenic species, although the overall pattern of hepcidin expression was in accordance with that generally observed in battery genes appeared during early phase of inflammation. Fish challenged with E. coli (non-pathogenic) showed the significant induction of hepcidin transcripts within 24 hr post injection (hpi) but the level was rapidly declined to the basal level either at 48 or 96 hpi. On the other hand, hepcidin transcript levels in E. tarda (pathogenic)-challenged fish were continuously elevated until 48 hpi, then downregulated at 96 hpi, although the level at 96 hpi was still significantly higher than control level observed in non-challenged fish. This expression pattern was consistent in all the three tissues examined. Taken together, our data indicate that hepcidin is tightly in relation with pathological and/or inflammation status during bacterial challenge, consequently providing useful basis to extend knowledge on the host defensive roles of hepcidin under infectious conditions in bony fish.