• 생명과학회지
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• 제19권10호
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• pp.1389-1395
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• 2009

천연물 신약으로 응용 가능성이 있는 목초액을 산화응집반응과 활성탄 등을 첨가하여 증류하는 방법으로 제조하였으며, 장기보존과 가속 저장 조건에서 목초액의 이화학적 변화(pH, 비중, 굴절률 및 용해 타르), 유해물질(카르보닐기, 벤조피렌 및 잔류용매) 그리고 유기산(개미산, 초산 및 프로피온산)의 변화를 검토하여 안정성을 평가하였다. 또한, 발모 모델로서 C57BL/6 마우스를 이용하여 모발 성장 효과를 살펴보았다. 보관 기간 중에 어떤 화학 결합이 일어나거나 또는 분해반응이 반복적으로 일어나지 않았다. 또한, 유해성분이나 주성분이 일정하게 유지되었고 특히 발암물질인 벤조피렌은 검출되지 않았다. 정제된 목초액 또는 미녹시딜을 2주 동안 등에 도포한 후, 어떤 임상 증상 없이 음성 대조군인 생리식염수보다 모발 성장을 촉진하였다. 따라서 화장품이나 천연물 신약으로 응용가능성이 높은 목초액을 함유한 제품 개발 시 일정한 양으로 유지되는 주성분을 함유하면서 페놀과 같은 유해물질을 줄일 수 있는 방법이 가능할 뿐만 아니라 모발 성장 촉진 효과가 있어 탈모환자에도 유용하게 사용될 것으로 기대되어진다.

• 한국식품영양학회지
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• 제22권3호
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• pp.430-442
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• 2009

This study was designed to investigate the relationship between the dietary intake according to calorie intake from a coffee containing beverage and the bone health status of 189 female collegians. The study was conducted through questionnaires, anthropometric checkup, 3-days food records and ultrasound measurement of calcaneus bone mineral density. Subjects were divided into three groups: students not drinking coffee(non-coffee group, N=56), students consuming <100 kcal daily from coffee(low-calorie coffee group, N=84), and students consuming $\geq100$ kcal of their total daily calories from coffee(high- calorie coffee group, N=49). There were no significant differences in weight, height, body mass index, body fat and calcaneus bone mineral density among the three groups. The low-calorie coffee group usually drank black coffee or instant coffee mix, and the high-calorie coffee group habitually drank coffee with milk or sugar syrup. There were no significant differences in the mean daily energy and food intake among the three groups. However, vitamin $B_2$(p<0.05) and calcium (p<0.01) intake in the high-calorie coffee group were higher than in the non-coffee group. Also, mean intake of sugars, fish and shellfishes, milks and beverages in the high-calorie coffee group were also significantly higher than in non coffee group(p<0.05). There was no significant difference in the Korean Dietary Diversity Score(KDDS) among the three groups. The main calcium source was milk in all three groups, and milk intake(total and included with coffee) was highest in the high-calorie coffee group. Although no significant difference was apparent between the high-calorie and non-coffee groups concerning anthropometric factors and calcaneus bone mineral density, consumption of coffee may have influenced food and nutrient intake. The results suggest that consumption of milk-supplemented coffee may be of nutritional benefit.

• 지질공학
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• 제26권3호
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• pp.371-382
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• 2016

해안지역의 염수침입은 지하수내 다양한 지화학 성분들에 의해서 탐지될 수 있다. 그러나 불충분한 지하수 자료는 염수침입의 인지를 어렵게 한다. 확률밀도함수는 제한된 수질자료를 이용하여 더 넓은 범위의 확률적 예측이 가능하며 특성화된 확률밀도 분포 도출을 통하여 해안지역의 염수침입을 효과적으로 판단할 수 있다. 본 연구에서는 부산시의 해안지역 지하수, 해안유출수, 하천수의 화학성분에 대해서 확률통계기법을 적용함으로써 염수침입을 평가하였다. 이 연구에 의해서, 해수 중에 농도가 높은 항목인 Na⁺, Mg²⁺, K⁺, SO₄²⁻, Cl⁻ 은 염수의 영향유무 판별의 유용한 지시자인 것으로 판명된다. 한편, 지하수, 해안유출수 및 하천수에서 유사한 확률분포형태를 보이는 항목인 Si⁴⁺, Fe²⁺, NO₃⁻, PO₄³⁻은 염수영향에 대한 지시자로서 적당하지 않은 것으로 판단된다.

• 한국수정란이식학회지
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• 제28권3호
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• pp.243-250
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• 2013

Pyracantha is a genus of thorny evergreen large shrubs in the family of Rosaceae, with common names Firethorn or Pyracantha. It's extract has also been used in cosmetics as a skin-whitening agent and functioning through tyrosinase inhibition. Recent studies have shown that pyracantha extract possesses antioxidant activities and may significantly improve lipoprotein metabolism in rats. Although the mode of action of Pyracantha extract is not fully understood, a strong relationship was observed between antioxidant and apoptosis in some types of cells. Thus, the aim of this study was to evaluated the effect of pyracantha extract on blastocysts formation and their quality of the porcine parthenogenetic embryos. After parthenogenetic activation by chemicals, presumptive porcine parthenogenetic embryos were cultured in PZM-3 medium supplemented with extracts of pyracantha leaf, stalk and root for 6 day (1, 5 and $10{\mu}g/ml$, respectively). In our results, the frequency of blastocyst formation in pyracantha root extract ($5{\mu}g/ml$) treated group had increased that of other groups. Furthermore, blastocysts derived from pyracantha root extract ($5{\mu}g/ml$) treated group had increased the total cell numbers and reduced apoptotic index. Blastocyst development was significantly improved in the pyracantha root extract ($5{\mu}g/ml$) treated group when compared with the $H_2O_2$ treated group (p<0.05). Subsequent evaluation of the intracellular levels of ROS in pyracantha root extract ($5{\mu}g/ml$) treated groups under $H_2O_2$ induced oxidative stress were decreased (p<0.05). In conclusion, our results indicate that treatment of pyracantha root extract may improve in vitro development of porcine parthenogenetic embryos through its antioxidative and antiapoptotic effects.

• 대한후두음성언어의학회지
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• 제26권2호
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• pp.117-121
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• 2015

Background : Cepstral analysis which is obtained from Fourier transformation of spectrum has been known to be effective indicator to analyze the voice disorder. To evaluate the voice disorder, phonation of sustained vowel /a/ sound or continuous speech have been used but the former was limited to capture hoarseness properly. This study is aimed to compare the effectiveness in analysis of cepstrum between the sustained vowel /a/ sound and continuous speech. Methods : From March 2012 to December 2014, total 72 patients was enrolled in this study, including 24 unilateral vocal cord palsy, vocal nodule and vocal polyp patients, respectively. The entire patient evaluated their voice quality by VHI (Voice Handicap Index) before and after treatment. Phonation of sustained vowel /a/ sample and continuous speech using the first sentence of autumn paragraph was subjected by cepstral analysis and compare the pre-treatment group and post-treatment group. Results : The measured values of pre and post treatment in CPP-a (cepstral peak prominence in /a/ vowel sound) was 13.80, 13.91 in vocal cord palsy, 16.62, 17.99 in vocal cord nodule, 14.19, 18.50 in vocal cord polyp respectively. Values of CPP-s (cepstral peak prominence in text-based speech) in pre and post treatment was 11.11, 12.09 in vocal cord palsy, 12.11, 14.09 in vocal cord nodule, 12.63, 14.17 in vocal cord polyp. All 72 patients showed subjective improvement in VHI after treatment. CPP-a showed statistical improvement only in vocal polyp group, but CPP-s showed statistical improvement in all three groups (p<0.05). Conclusion : In analysis of cepstrum, text-based analysis is more representative in voice disorder than vowel sound speech. So when the acoustic analysis of voice by cepstrum, both phonation of sustained vowel /a/ sound and text based speech should be performed to obtain more accurate result.

• 한국가금학회지
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• 제40권3호
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• pp.223-234
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• 2013

Poultry industry is abounding day by day as it engrosses less cost of investment per bird as compared to large animals. Poultry have the most copious genomic tool box amongst domestic animals for the detection of quantitative trait loci (QTL) and marker assisted selection (MAS). Use of multiple markers and least square techniques for mapping of QTL affecting quality and production traits in poultry is in vogue. Examples of genetic tests that are available to or used in industry programs are documented and classified into causative mutations (direct markers), linked markers in population-wide linkage disequilibrium (LD) with the QTL (LD markers), and linked markers in population wide equilibrium with the QTL (LE markers). Development of genome-wide SNP assays, role of 42 K, 60 K (Illumina) and 600 K (Affymetrix$^{(R)}$ Axim$^{(R)}$) SNP chip with next generation sequencing for identification of single nucleotide polymorphism (SNP) has been documented. Hybridization based, PCR based, DNA chip and sequencing based are the major segments of DNA markers which help in conducting of MAS in poultry. Economic index-marker assisted selection (EI-MAS) provides platform for simultaneous selection for production traits while giving due weightage to their marginal economic values by calculating predicted breeding value, using information on DNA markers which are normally associated with relevant QTL. Understanding of linkage equilibrium, linkage dis-equilibrium, relation between the markers and gene of interest are quite important for success of MAS. This kind of selection is the most useful tool in enhancing disease resistance by identifying candidate genes to improve the immune response. The application of marker assisted selection in selection procedures would help in improvement of economic traits in poultry.

• 대한예방한의학회지
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• 제8권1호
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• pp.19-32
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• 2004

This study was conducted to evaluated musculoskeletal subjective symptoms and the degree of stress of industrial workers to present fundamental materials of preventive oriental medicine for improving their health and quality of life. During the medical examination with oriental medicine method, presence of subjective symptoms of musculoskeletal pain, Psychosocial Well-being Index(PWI) and life style were checked by using questionnaire method in 474 industrial workers. The collected data were analyzed with crosstabs, ANOVA and T-test. The results were as follows; 1. In general differences according to musculoskeletal subjective symptoms, education level of high school graduation had significantly higher distribution than that of below middle school or above university graduation in the pain present group. 2. In the musculoskeletal subjective symptoms and the degree of stress, all of Factor1, Factor2. Factor3, Factor4. and PWI had higher score in pain present group than in pain free group and the difference was statistically significant. 3. According to degree of stress and general character of subjects, single and education level of high school graduation had high score in Factor2, Factor3 and PWI. Factor1 and Factor3 was high in income group of low 1.49 million won. Factor2, Factor4, and PWI was high significantly in income group of 1.50 - 2.99 million won. In job type, manufacturing worker group had significantly high score in Factor3. 4. In degree of stress and life style difference, there was significant difference in PWI score in the field of alcohol chinking, smoking, exercise, obesity except sleeping hours. In the present study. as a result, it is found out that musculoskeletal subjective symptoms present group had higher score in psychosocial stress across the fields than symptom-free group and it is recommended that continuous studies on the relationship of job-related musculoskeletal disorders and psychosocial stress should be performed for improvement and prevention of mental and physical health of industrial worke

• 한국방사선학회논문지
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• 제9권7호
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• pp.467-471
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• 2015

본 연구에서는 L-tube insertion 환자의 흉부 AP 검사 시 tube tip이 환자의 자세나 방사선 조사각에 의해 왜곡되는 정도를 파악하고 정확한 임상정보를 제공하기 위해 검사 시 정확한 tube 각도를 유지하여 영상의 질을 높이고자 하는데 있다. 실험 장비로 SHIMADZU사의 ELMO-T6S를 사용하였으며, Chest phantom의 표면에 1 mm 간격의 눈금격자를 부착한 L-tube를 부착하여 실험을 하였다. 실험영상의 영상 획득 조건은 90 kVp, 4 mAs, SSD 120 cm로 하여 영상을 획득하였다. Phantom position은 table에서 supine, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$로 변화시키고 각 position마다 Head 방향과 Feet 방향으로 수직, ${\pm}5^{\circ}$, ${\pm}10^{\circ}$, ${\pm}15^{\circ}$ 촬영을 하여 영상을 획득하였다. 본 실험 결과로 L-tube tip의 위치는 환자의 자세와 방사선 입사각에 따라 달라지며 환자의 position이 30, 45, $60^{\circ}$일 때 보다 supine 일 때 tip의 위치변화가 적은 것으로 나타났다. 흉부 방사선 검사를 통해 L-tube tip의 정확한 위치를 보고자 할 때 잘못 된 입사각에 의한 영상의 왜곡이 발생하지 않도록 환자의 자세나 입사각을 조정해야 하며, 해당 시술을 확인하는데 정확한 평가 지표로 이용될 수 있도록 임상에서 업무를 수행하는 방사선사들의 세심한 노력이 필요할 것으로 사료된다.

• 대한지역사회영양학회지
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• 제18권1호
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• pp.77-87
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• 2013

This study was conducted to investigate the nutrient intakes of subjects by quartile of percent energy intake from cooked rice, consumption of cooked rice mixed with multi-grains and to evaluate rice consumption in relation to the risk of metabolic syndrome. The subjects were 5,830 males and females aged between 20~64 years based on 2007-2008 KNHNES data. Levels of percent energy intake from cooked rice were classified into 4 groups (Q1, Q2, Q3, Q4 groups: 25% of each) using data of 24-hour recall method from KNHNES. Using medical examination and questionnaire, subjects were classified according to diagnostic criteria of metabolic syndrome. The subjects with higher age, being married, lower education, lower economic level were more likely to take higher percent energy intake from cooked rice. Quartile Q3 of percent energy intake from cooked rice tended to show higher Index of Nutritional Quality (INQ) for fiber, calcium, iron, potassium and vitamin A. INQ of protein, dietary fiber, calcium, thiamin, phosphorus, potassium, riboflavin, niacin and vitamin C by consumption of cooked rice mixed with multi-grains was higher than that by consumption of cooked white rice when adjusted for age. No association with a risk for metabolic syndrome was found for quartile of percent energy intake from cooked rice or cooked rice mixed with multi-grains compared to cooked white rice after adjusting for energy, gender, age, BMI, alcohol, smoking, income and physical activity. In conclusion, consumption of over 54% energy intake from cooked rice or only cooked white rice showed relatively low INQs, but was not associated with a higher risk for metabolic syndrome.

• 환경생물
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• 제27권1호
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• pp.31-39
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• 2009

농업생태계의 환경별로 깔따구류의 분포를 밝히고 우점종을 이용한 유기물 분해능을 평가하고자 대단위경작지, 친환경농업지, 생활하수 유입지, 축산폐수 유입지 및 주변수로 등의 5개 서식처별로 나누어 '04$\sim$'06년까지 각 구획을 대표하는 총 78개 지점에서 깔따구과 및 공서 무척추동물을 정량 채집하였다. 그 결과, 농업지대의 깔따구류는 총 16종으로 동정되었다. 서식지 유형별로 출현한 종수를 보면, 축산폐수 유입지에서 11종, 대단위 농경지는 8종, 농경지 주변수로는 7종, 친환경농업지는 6종, 생활하수 유입지는 2종으로 조사되었다 구획별로 나타난 우점종을 보면, 유기물의 농도가 높은 대단위경작지(A), 생활하수 유입지(C) 및 축산폐수 유입지(D)에서는 닙폰깔따구(C. nipponensis), 유기물 함량이 낮은 주변수로(E)에서는 숲아기깔따구(C. sylvestris), 친환경농업지(B)에서는 서산장부깔따구(Tanytarsus sesanensis)였다. 수질과 군집지수는 논주변의 수로에서 높았고, 대단위 경작지, 친환경농업지, 생활하수 유입지, 축산폐수 유입지는 낮은 수준으로 조사되었다. 저질내에서 닙폰깔따구의 유기물 제거능력은 무처리구와 대비하여 90개체 처리구에서 18% 증가하였다.