• 제목/요약/키워드: putAP

검색결과 12건 처리시간 0.022초

Regulatory Characteristics of the Vibrio vulnificus putAP Operon Encoding Proline Dehydrogenase and Proline Permease

  • Lee Jeong-Hyun;Jeong So-Young;Choi Sang-Ho
    • Journal of Microbiology and Biotechnology
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    • 제16권8호
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    • pp.1285-1291
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    • 2006
  • The proline utilization (put) operon of Vibrio vulnificus consists of the putAP genes encoding a proline dehydrogenase and proline permease. The result of put-lux transcriptional fusion analysis suggests that the V vulnificus putAP operon is not autoregulated by the PutA protein. A putR null mutation decreased proline dehydrogenase activity and the level of the put transcripts, indicating that transcription of putAP is under the positive control of PutR. The deduced amino acid sequence of the putR was similar to those reported from other bacteria with high levels of identity. Chromatin IP and GST pull-down assays revealed that PutR specifically binds to the putAP promoter region in vivo, and interacts with CRP in vitro. Taken together, the results suggested that PutR exerts its effect on putAP expression by directly interacting with CRP bound to the upstream region of P$_{put}$.

Identification and Functional Analysis of the putAP Genes Encoding Vibrio vulnificus Proline Dehydrogenase and Proline Permease

  • Kim, Hye-Jin;Lee, Jeong-Hyun;Rhee, Jee-Eun;Jeong, Hye-Sook;Choi, Hyun-Kyung;Chung, Hee-Jong;Ryu, Sang-Ryeol;Choi, Sang-Ho
    • Journal of Microbiology and Biotechnology
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    • 제12권2호
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    • pp.318-326
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    • 2002
  • The pathogenic marine bacterium Vibrio vulnificus is the causative agent of food-borne diseases such as life-threatening septicemia. To better understand this organism's strategies to survive osmotic stress, a mutant that was more sensitive to high osmolarity was screened from a library of mutants constructed by a random transposon mutagenesis. By a transposon-tagging method, putAP genes encoding a proline dehydrogenase and a proline permease were identified and cloned from V. vulnificus. The amino acid sequences deduced from nucleotide sequences of putAP from V. vulnificus were 38 to $59\%$ similar to those of PutA and PutP reported from other Enterobacteriaceae. Functions of putAP genes were assessed by the construction of mutants, whose putAP genes were inactivated by allelic exchanges. When proline as the sole carbon or nitrogen source was used, the putA mutant was not able to grow to the substantial level, revealing the proline dehydrogenase is the only enzyme for metabolic conversion of proline into other amino acids. Although the growth rate of the putP mutant on proline as the sole carbon or nitrogen source was significantly reduced, the mutant still grew. This indicated that at least one more proline permease is produced by V. vulnificus. The putP mutant decreased approximately $2-log_10$ CFU/ml after a hyperosmotic challenge, while the parent strain decreased approximately $l-log_10$ CFU/ml. This result suggests that the gene product of putP contributes to the osmotic tolerance of V. vulnificus.

WiFi AP 성능 향상을 위한 무선 자원 관리 최적화 (RRM Optimization for the Throughput Enhancement of WiFi AP)

  • 정길현
    • 한국컴퓨터정보학회논문지
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    • 제17권12호
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    • pp.131-136
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    • 2012
  • 최근 스마트 이동 단말기기의 급속한 보급과 더불어 많은 수의WiFi Access Point (AP)가 설치되어 사용되고 있으며, WiFi AP의 증가는 AP 간의 상호 간섭에 의한 트래픽 처리 성능의 저하를 발생시킨다. 이런 현상은 기존 WiFi 망에 활용할 수 있는 트래픽 성능을 향상시키기 위한 방법을 요구한다. 본 연구에서는 트래픽 성능을 향상시키기 위하여 AP의 무선 자원 관리 (RRM : Radio Resource Management)를 최적화하기 위한 채널 할당 방법과 분산형 Wireless LAN (WLAN) 에서의 트래픽 성능 향상 방법에 대하여 연구하였다. 그 결과, AP 단독으로 실행되어 기존방식의 할당 오류를 개선하고 실행속도를 증가시킬 수 있었다.

Performance of Seamless Handoff Scheme with Fast Moving Detection

  • Kim Dong Ok;Yoon Hong;Yoon Chong Hoo
    • 대한전자공학회:학술대회논문집
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    • 대한전자공학회 2004년도 학술대회지
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    • pp.588-591
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    • 2004
  • This paper describes a new approach to Internet host mobility. We argue that local mobility, the performance of existing mobile host protocol can be significantly improved. It proposes Fast Moving Detection scheme that based on neighbor AP channel information and moving detection table. And, it composes Local Area Clustering Path (LACP) domain that collected in AP's channel information and MN interface information. It stored the roaming table to include channel information and moving detection. Those which use the proposal scheme will need to put LACP information into the beacon or probe frame. Each AP uses scheme to inform available channel information to MN. From the simulation result, we show that the proposed scheme is advantageous over the legacy schemes in terms of the burst blocking probability and the link utilization.

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무선 랜에서 우선순위를 할당한 다중 버퍼 사용에 따른 성능 향상에 관한 연구 (The Study for Improvement Performance on Priority Multi-Buffer Scheme in Wireless LAN)

  • 김동옥
    • 정보통신설비학회논문지
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    • 제3권2호
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    • pp.42-50
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    • 2004
  • This paper describes a new approach to Internet host mobility. We argue that local mobility, the performance of existing mobile host protocol can be significantly improved. It proposes Fast Moving Detection schemethat based on neighbor AP channel information and moving detection table. And, it composes Local Area Clustering Path (LACP) domain that collected in AP's channel information and MN interface information. It stored the roaming table to include channel information and moving detection. Those which use the proposal scheme will need to put LACP information into the beacon or probe frame. Each AP uses scheme to inform available channel information to MN. From the simulation result, we show that the proposed scheme is advantageous over the legacy schemes in terms of theburst blocking probability and the link utilization.

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4차원 방사선 치료시 Body Fix의 유용성 평가 (Evaluation on Usefulness of Applying Body-fix to Four Dimensional Radiation Therapy)

  • 김영재;장영일;지연상;한재복;최남길;장성주
    • 한국콘텐츠학회논문지
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    • 제13권10호
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    • pp.419-426
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    • 2013
  • 본 연구에서는 간암 치료시 Body fix를 이용하여 4DRT를 실시할 경우 종양의 체적, 위치의 변화정도, 종양 및 정상조직의 선량흡수정도를 파악하여 Body fix의 유용성을 평가하였다. 사전 연구에 동의한 23명의 환자를 대상으로 Body fix의 착용 유무에 따라 각기 다른 4차원 모의치료영상을 획득하여 영상을 바탕으로 종양조직 및 정상조직을 설정하고 분석하였다. 종양의 용적을 분석해본 결과 Body fix의 사용이 GTV는 0.17%, CTV와 PTV는 3.2% 정도 체적을 줄일 수 있었으며, 종양의 운동성은 AP방향에서 29.8%, UL방향에서 5.31%의 움직임을 감소시킬 수 있었다. 종양의 흡수선량 값은 Body fix를 사용한 경우가 다소(1.3%) 높았으며 정상조직(정상간, 위, 우측신장, 척수)의 흡수선량은 5% 정도 줄일 수 있었다. 따라서 간암의 4DRT시 Body fix는 유용하게 사용할 수 있을 것으로 생각된다.

설악산 식물군락 유기물층의 화분분석학적 연구 (Pollen Analytical Study on Humus Accumulated at Plant Communities of Mt. Seolag)

  • 강상준
    • Journal of Plant Biology
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    • 제30권4호
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    • pp.267-276
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    • 1987
  • A pollen analytical study was carried out on the accumulated humus samples collected from 27 sites under the different plant communities of Mt. Seolag. These pollen spectra were then compared with the actual vegetation. The aboreal pollen(AP) and non-abnoreal pollen(NAP) were 1 Family 25 Genus and 5 Family 5 Genus, respectively. Among the aboreal pollen identified, the pollen of Pinus, Quercus, Betula and Acer were appeared in abundance and the coniferous pollen of Pinus was occupied at least 20%-65% through all the sites studied. The pollens of Abies, Betula, accer, Quercus, Carpinus, Corylus, Fraxinus, Styrax, Prunus and Ericaceae were in accordance with the present vegetation but the other pollens were inconsistent with the actual vegetaton. It suggests that it was caused by the difference between Wehseolag and Namseolag of subalpine zone in Mt. Seolag was 200 m by pollen spectra. Quercus was distributed at the lower part of Namseolag and the subalpine boreal elements were increased with increasing altitudes. The pollen spectra of each site was put together into 5 groups of pollen assemblage by cluster analysis and the community coefficient (CC) was over 60% between all sites studied.

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Mobile Chest AP 검사 시 환자자세와 입사각도에 따른 Levin-tube tip의 영상왜곡 분석 (The Image Distortion Analysis of Levin-tube tip by Patient position and Incidence Angle when taking Mobile Chest AP Projection)

  • 이진수;박형후
    • 한국방사선학회논문지
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    • 제9권7호
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    • pp.467-471
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    • 2015
  • 본 연구에서는 L-tube insertion 환자의 흉부 AP 검사 시 tube tip이 환자의 자세나 방사선 조사각에 의해 왜곡되는 정도를 파악하고 정확한 임상정보를 제공하기 위해 검사 시 정확한 tube 각도를 유지하여 영상의 질을 높이고자 하는데 있다. 실험 장비로 SHIMADZU사의 ELMO-T6S를 사용하였으며, Chest phantom의 표면에 1 mm 간격의 눈금격자를 부착한 L-tube를 부착하여 실험을 하였다. 실험영상의 영상 획득 조건은 90 kVp, 4 mAs, SSD 120 cm로 하여 영상을 획득하였다. Phantom position은 table에서 supine, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$로 변화시키고 각 position마다 Head 방향과 Feet 방향으로 수직, ${\pm}5^{\circ}$, ${\pm}10^{\circ}$, ${\pm}15^{\circ}$ 촬영을 하여 영상을 획득하였다. 본 실험 결과로 L-tube tip의 위치는 환자의 자세와 방사선 입사각에 따라 달라지며 환자의 position이 30, 45, $60^{\circ}$일 때 보다 supine 일 때 tip의 위치변화가 적은 것으로 나타났다. 흉부 방사선 검사를 통해 L-tube tip의 정확한 위치를 보고자 할 때 잘못 된 입사각에 의한 영상의 왜곡이 발생하지 않도록 환자의 자세나 입사각을 조정해야 하며, 해당 시술을 확인하는데 정확한 평가 지표로 이용될 수 있도록 임상에서 업무를 수행하는 방사선사들의 세심한 노력이 필요할 것으로 사료된다.

택사약침(澤瀉藥鍼)의 항산화효과(抗酸化效果)에 관(關)한 실험적(實驗的) 연구(硏究) (The Experimental Study about Antioxidant Activities of Alismatis Rhizoma Herbal Acupuncture)

  • 이종무;이병렬
    • Journal of Acupuncture Research
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    • 제20권1호
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    • pp.159-176
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    • 2003
  • Objective : The purpose of this study is to investigate the antioxidant activities of Alismatis Rhizoma herbal acupuncture by experimental methods. Methods : For this purpose, first, we put an emphasis in the control of enzymes of the antioxidant system in various changes inside the cell; these changes caused by the proliferation or the activation of the cell which were brought about by the handling of PMA and $TNF-{\alpha}$ into the THP-1 monocyte cell of the body each other. After that, we caused the acute oxidant symptom by the injection of AAPH into the mouse' abdominal cavity, and then applied the herbal acupuncture on S36 point(足三里), and finally, we measured the change of blood ingredient and the resistance against the activated oxygen of the red blood cell membrane, MDA, SOD, and catalase. Results : In vitro the revelation of $IL-1{\beta}$, IL-8, $TNF-{\alpha}$, NOS II and IL-6 were decreased and the revelation of IL-10, $TGF-{\beta}$, GM-CSFIL-12, GM-CSF and SOD were increased. The DNA-binding of $NF-{\kappa}B$ and AP-1 were activated and the formation of ROS in the THP-1 cell line was decreased. In vivo $IL-1{\beta}$ among producing the cytokine inside the plasma was meaningfully dwindled and the $INF-{\gamma}$ was meaningfully increased. The resistance of red blood cell membrane against the activated oxygen was meaningfully increased and the MDA formation was meaningfully dwindled, In the activation of hepatic antioxidase, the SOD was meaningfully increased. Conclusion : Alismatis Rhizoma herbal acupuncture by experimental methods has effected on the antioxidant activities.

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