• Journal of Korean Society of Forest Science
• /
• v.79 no.3
• /
• pp.278-284
• /
• 1990

We have demonstrated expression of bacterial genes transferred into cells of Populus nigra ${\times}$ P. maximowiczii by A. tumefaciens strain 6044 (pGA 472). We determined the optimum concentration of kanamycin sulfate for effective selection of punctured leaf transformed using Agrobacterium binary vector pGA 472 containing a neomycine phosphotransferase gene (NPT-II) which confers kanamycin resistance. The combination of cefotaxime (200mg/l) and carbenicillin (300mg/l) showed good performance of discarding Agrobacterium from inoculated punctured leaf. A relatively low concentration (10mg/l) of kanamycin sulfate inhibited callus and shoots induction from punctured leaf. Number of shoots regenerated from co-cultured punctured leaf was 3.0 on MS basal medium supplemented with 10 mg/l kanamycin sulfate, while that of not co-cultured punctured leaf was none. The regeneration rate was 10% from the punctured leaf co-cultured on MS medium with 10 mg/l kanamycin. Regenerated shoots are developing from micropropagation for Southern blot analysis and inheritance of the kanamycin resistance trait (NPT-II).

• Korean journal of applied entomology
• /
• v.21 no.4 s.53
• /
• pp.175-178
• /
• 1982

Field studies on the damage type of rice plants by the rice stem maggot in the second generation were conducted at Yong-in, Gyonggi Province, in 1982. Rate of damaged ears and the number of punctured leaves were higher in Tongil line than Japonica line. Early heading cultivars usually had bottom-damaged ears and on the contrary late heading cultivars had upper?damaged ears. In Tongil line, many of the upper-damaged or middle-damaged ears had punctured-flag leaves and less first leaves with punctures, but most of the bottom-damaged ears had not punctured-leaves. When matured, the larvae moved up to the upper part of stem and pupated on the upper and inner part of leaf-sheath of flag or first leaves.

• Horticultural Science & Technology
• /
• v.17 no.4
• /
• pp.485-488
• /
• 1999

This study was conducted to obtain a basic information on the structural and histological characteristics of Gerbera hybrida Hort. leaf injured by american serpentine leafminer (ASL), Liriomyza trifolii, by using light and scanning electron microscope (SEM). Based on the anatomical observation of leaf blade injured by L. trifolii, the injury process could be divided into three stages. In the initial stage, the punctured tiny holes where ASL layed eggs after suction in the upperside of leaf were observed in the palisade parenchyma. In the middle stage, the hatched larvae made mines in the palisade parenchyma only. In the final stage, the mature larvae grew up making the mines bigger, and just before going out from the epidermis, it injured the inside of leaf containing one layer palisade parenchyma and two layers of spongy parenchyma.

• Korean Journal of Plant Tissue Culture
• /
• v.22 no.6
• /
• pp.329-334
• /
• 1995

Transformation system of rolC gene, dwarf gene in Lycium chinenese Mill. established by using system. Pin-punctured leaves induced numerous adventious buds in abaxial side when cultured on 3/2 MS medium containing 2.0 mg/L zeatin. Survival rate and shoot regeneration frequency of leaf explants decreased as kanamycin sulfate level increased. Shoot buds were not regenerated on 3/2 MS medium containing 10 mg/L kanamycin sulfate and 2.0 mg/L zeaein. Of the level tested, 10 mg/L of kanamycin sulfate was optimum in selection of kanamycin sulfate resistant plant. Co-culture time of bacteria and leaf explants was affected at the frequency of shoot regeneration and survival of leaf explants. Leaf explants co-cultivated during above 48hr severely decreased survival rate and shooting rate. Best result on survival rate and shooting rate were obtained when exposed for 24 h. 80 explants of 105 leaf explants survived on 3/2 MS medium containing 2.0 mg/L zeatin and 10 mg/L kanamycin sulfate, and 15 shoots was regenerated on the same medium. To select kanamycin sulfate resistant plant, regenerate as cultured on 3/2 MS medium containing 10 mg/L kanamycin sulfate, and obtained 5 kanamycin resistant plants. Southern blot analysis conformed that the rolC gene was incorporated into the genomic DNA of kanamycin resistant plants.

• Journal of Korean Society of Forest Science
• /
• v.84 no.1
• /
• pp.41-47
• /
• 1995

A micropropagation system for black Locust(Robinia pseudoacacia) was established by using shoots and pin-punctured leaves of in vitro germinated seedlings. The greatest number of shoots (an average of 10.5 shoots) was obtained when shoot tips were cultured on MS medium supplemented with 1.0 mg/l BAP and 0.01 mg/l NAA. When pin-punctured leaf explants were cultured on the same medium, mean number of 13.5 shoots were produced. Shoot growth was accelerated by adding 50 mg/l of silver nitrate ($AgNO_3$), an anti-ethylene compound to the culture medium. Each shoot was excised from the mass and transferred onto half strength MS medium for rooting. Zygotic embryos at different developmental stages were cultured on LS medium supplemented with various growth regulators to induce somatic embryos. When cultured on LS medium with 1.0 mg/l 2,4-D. 14.3% of the zygotic embryos induced somatic embryos. Upon transfer onto the basal medium, somatic embryos sporadically converted into plantlets.