• Advances in pediatric surgery
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• 제4권2호
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• pp.172-175
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• 1998

With the recent advances in instruments and techniques, laparoscopic procedure have extended to neonates with congenital anomalies. The author reports a 6-day-old boy with Hirschsprung's disease, treated successfully by the laparoscopic endorectall pull-through procedure. The technique and its potential role in the treatment of Hirschsprung's disease are described. One camera port and three working ports were used for access to the peritoneal cavity. The descending and sigmoid colon were mobilized laparoscopically. The submucosal dissection was done transanally. The colon was then pulled down in continuity, divided above the transition zone, and secured to the anal mucosa about 10 mm above the pectinate line. Author concluded that endorectal pull-through can be performed safety with the laparoscope.

• 대한전자공학회논문지
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• 제26권2호
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• pp.102-111
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• 1989

본 논문에서는 두 가지의 새로운 형태의 non-inverting BiCMOS 구동회로를 제안한다. 이들은 pull-dwon용 바이폴라 트랜지스터의 베이스를 pMOS 트랜지스터를 통해 충전시킴을 그 특징으로 한다. 이들의 pull-up시의 지연시간은 기존의 BiCMOS 구동회로들보다 20%~47% 작다. 또한 본 논문에서 제안하는 구동회로에 사용되는 MOS 트랜지스터의 갯수는 기존의 구동회로에서보다 1~3개가 적기 때문에 그 차지하는 면적이 감소된다.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2008년도 International Meeting on Information Display
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• pp.1301-1304
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• 2008

In this study, we used a multiple phase scheme for the clock in the dual-pull-down driver for TFT display panels. In this scheme, the turn-on time for the transistors in the dual-pull-down structure was reduced from 1/2 to 1/4 or 1/8 of the period cycle time. While keeping proper operation of the transistor size of circuit was fine tuned to achieve an optimal performance. The relation between the active time and the transistor dimensions was obtained for the optimal design.

• 대한전기학회논문지:전기물성ㆍ응용부문C
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• 제50권9호
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• pp.471-475
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• 2001

ln this paper, a micromachined micromechanical switch is presented. The presented switch is operated in the vertical direction to the substrate by an electrostatic force between two parallel plates. The moving plate is pulled down to connect the bumps of the bias node$(V_{DD}/ or GND)$ to the bumps of the output node when a oltage difference exists between the moving plate and the input plate. The switch was designed to operate at a low switching voltage$(\risingdotseq5V)$ by including a large-area, narrow-gap, parallel plate capacitor A theoretical analysis of the designed switch was performed in order to determine its geometry fitting the desired pull-in voltage and release voltage. The designed switch was fabricated by surface micromachining combined with Ni electroplating. From the experimental results of the fabricated switch, its pull-in voltage came Out to be less than 5V and the measured maximum allowable current was 150mA. The measured average ON-state resistance was about 8$\Omega$, and the OFF-state resistance was too high to be measured with digital multimeter.

• 한국철도학회:학술대회논문집
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• 한국철도학회 2008년도 춘계학술대회 논문집
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• pp.332-338
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• 2008

This paper handles, implementation of multiple access bidirectional serial communications protocol using two common DC power lines, whict are power supply and ground, connecting multiple devices. Communication between the host and the multiple clients are performed using unique packet data with device ID unique to each devices connected on the common power lines. Host initiates data communications by transmitting command packet to the designated client with the client's ID and the client responds by transmitting response packet to the host and in this way, multiple clients and host exchange the packet through the common power lines. The normal voltage of the power communication line maintains 24V corresponding to level 1 and the host drops the voltage to 12V on sending level 0 signal, also the clients normally keeps the line voltage to 24V use pull-down circuit to drop the voltage to 12V on sending level 1 signal. Power supply originates from the host, the host senses the voltage level of the power communication lines and when the clients activates pull down circuit to send level 0 signal and the voltage drops to 12V, the hosts switches power source from 24V to 12V. Also, when clients deactivate pull down circuit to send level 1 signal, the host senses the voltage increase and switches the power source from 12V to 24V. Experimental circuit is designed with one hosts and four clients and verified the power line voltage switching operation depending on the data signal levels on the power line. The proposed research result can be applied to two wire power communications system with one host and multiple low current consumption clients.

• 생명과학회지
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• 제23권11호
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• pp.1311-1316
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• 2013

hnRNP L은 pre-mRNA에 결합하는 단백질들 중에서 핵심이 되는 단백질이다. hnRNP L은 양이 아주 많은 핵 단백질로서 핵과 세포질을 왕복하는 특성을 지니고 있다. 이 단백질은 염색질 변형(chromatin modification), pre-mRNA 스플라이싱, 인트론이 없는 유전자들에서 유래한 mRNA들의 세포질로의 반출(export), IRES-매개성 번역, mRNA의 안정성 조절, 정자형성과정 등, 세포 내의 여러 가지 과정에 관여하고 있는 것으로 알려져 있다. 이 논문에서는 hnRNP L과 결합하는 세포 내 단백질을 찾아내기 위하여 사람의 간세포 cDNA library를 사용하여 이스트 two-hybrid 탐색 실험을 수행하였다. 그 결과 사람의 간세포에서 IGFBP-4가 hnRNP L과 상호결합하는 새로운 파트너라는 것을 발견하였다. 본 연구를 통하여 hnRNP L이 이스트 two-hybrid 시스템에서 IGFBP-4와 특이적으로 상호 결합한다는 것을 처음으로 발견하였다. 본 연구에서는 또한 이스트 two-hybrid 시스템에서 hnRNP L이 IGFBP-4와 상호결합한다는 점을 in vitro pull-down 실험을 통하여 재확인하였다.

• Journal of Microbiology
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• 제44권5호
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• pp.577-580
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• 2006

GST pull-down assays were used to characterize the SARS-CoV membrane (M) and nucleocapsid (N) interaction, and it was found that the amino acids 211-254 of N protein were essential for this interaction. When tetrad glutamines (Q) were replaced with glutamic acids (E) at positions of 240-243 of the N protein, the interaction was disrupted.

• 대한의생명과학회지
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• 제9권4호
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• pp.231-240
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• 2003

Deciphering the molecular interactions of proteins forming Z-lines is pivotal for understanding the regulation of myofibril assembly, sarcomeric organization, and mechanical properties of striated muscle. The purpose of this study is to searched for potential novel ligands of the Z-line portion of nebulin. In this study interacting proteins with intra-Z-line region of nebulin were screened using a yeast two-hybrid approach. The interaction was conformed by GST pull-down assay. We identified 269 residues within villin/gelsolin homology domain of supervillin that intreacts with the serine rich region of nebulin. The specific interactions of nebulin and supervillin were confirmed in vitro by GST pull-down experiments. Our data suggest that supervillin attaches directly to the Z-line through its interaction with the serine rich domain of nebulin in skeletal muscles. This interaction may link myofibrillar Z-discs to the membrane-associated complexes, costameres.

• 대한전자공학회논문지
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• 제26권6호
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• pp.131-139
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• 1989

본 논문에서는 CMOS 및 domino CMOS 의 특징과 PLA등 array logic의 특징을 동시에 살리면서 동작특성이 좋고 집적도가 높으며 테스트 생성이 쉬운 domino CMOS NOR-NOR array logic의 설계방식을 제안하였다. 이 방식은 pull-down 특성을 개선하여 기생 커패시턴트의 문제점을 해결하며 간단한 부가회로를 사용하여 회로내의 모든 고정들을 검출할 수 있도록 한 testable design 방식이다. PLA의 적항군의 개념 및 특성 행렬을 이용한 테스트 생성 알고리듬과 절차를 제안하였고 이를 PASCAL 언어로 실현하였다. 또한 SPICE 및 P-SPICE를 이용하여 본 설계방식에 대한 검증을 행하였다.

• 대한의생명과학회지
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• 제12권4호
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• pp.303-310
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• 2006

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. We described here that the C-terminal domain of ankyrin-B interact specifically with Z-line portion of titin in yeast two-hybrid analysis, in vitro pull-down assays and localization experiments in COS-7 cells. In this study we provide the first experimental evidence that Z-line portion of titin is necessary for the localization of ankyrin-B and ankyrin-B links between the sarcolemma and the myofibril in costameres.