Biomedical Science Letters (대한의생명과학회지)

The Korean Society for Biomedical Laboratory Sciences (대한의생명과학회)
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C-Terminal Region of Ankyrin-B Interact with Z-Line Portion of Titin

  • Kim, Myong-Shin (Department of Biomedical Laboratory of Science, Inje University) ;
  • Kim, Hyun-Suk (Department of Biomedical Laboratory of Science, Inje University) ;
  • Park, Eun-Ran (Department of Biomedical Laboratory of Science, Inje University) ;
  • Lee, Yeong-Mi (Department of Biomedical Laboratory of Science, Inje University) ;
  • Lee, Min-A (Department of Biomedical Laboratory of Science, Inje University) ;
  • Kim, Ji-Hee (Department of Biomedical Laboratory of Science, Inje University) ;
  • Choi, Jae-Kyong (Department of Biology, Graduate School, Inje University) ;
  • Ahn, Seung-Ju (Department of Clinical Pathology, Daegu Health Collage) ;
  • Min, Byung-In (Department of Radiation Applied Engineering, Inje University) ;
  • Shon, Myeong-Hwan (Department of Orthopedic Surgery, Inje University) ;
  • Choi, Jang-Seok (Department of Orthopedic Surgery, Inje University) ;
  • Kim, Chong-Rak (Department of Biomedical Laboratory of Science, Inje University)
  • Published : 2006.12.31
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Abstract

Ankyrins are a ubiquitously expressed family of intracellular adaptor proteins involved in targeting diverse proteins to specialized membrane domains in both the plasma membrane and the endoplasmic reticulum. We described here that the C-terminal domain of ankyrin-B interact specifically with Z-line portion of titin in yeast two-hybrid analysis, in vitro pull-down assays and localization experiments in COS-7 cells. In this study we provide the first experimental evidence that Z-line portion of titin is necessary for the localization of ankyrin-B and ankyrin-B links between the sarcolemma and the myofibril in costameres.

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