• Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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• 2004.05a
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• pp.314-319
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• 2004

Many of researches regarding mechanical properties of composite materials are associated with humid environment and temperature. Especially the temperature is a very important factor influencing the design of thermoplastic composites. However, the effect of temperature on impact behavior of reinforced composites have not yet been fully explored. An approach which predicts critical fracture toughness GIC was performed by the impact test in this work The main goal of this work is to study effects of temperature in the impact test with glass fiber/polypropylene(GF/pp) composites. The critical fracture energy and failure mechanisms of GF/PP composites are investigated in the temperature range of $60^{\circ}C\;to\;-50^{\circ}C$ by impact test. The critical fracture energy shows a maximum at ambient temperature and it tends to decrease as temperature goes up or goes down. Major failure mechanisms can be classified such as fiber matrix debonding, fiber pull-out and/or delamination and matrix deformation.

• Bulletin of the Korean Chemical Society
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• v.33 no.9
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• pp.3071-3075
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• 2012

Human ${\alpha}$-synuclein is the major component of the protein aggregates known as Lewy bodies or Lewy neurites, which define the intracellular lesions of Parkinson's disease. Despite extensive efforts, the physiological function of ${\alpha}$-synuclein has not yet been elucidated in detail. As an approach to defining its function, proteins that interacted with ${\alpha}$-synuclein were screened in phage display assays. The SNARE protein vesicle t-SNARE-interacting protein homologous 1B (VTI1B) was identified as an interacting partner. A selective interaction between ${\alpha}$-synuclein and VTI1B was confirmed by coimmunoprecipitation and GST pull-down assays. VTI1B and ${\alpha}$-synuclein were colocalized in N2a neuronal cells, and overexpression of ${\alpha}$-synuclein changed the subcellular localization of VTI1B to be more dispersed throughout the cytosol. Considering the role played by VTI1B, ${\alpha}$-synuclein is likely to modulate vesicle trafficking by interacting with a SNARE complex.

• Journal of Environmental Science International
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• v.9 no.6
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• pp.443-447
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• 2000

Chonan and Asan city have been a focal point due to rapid development as the first station for Express Railroad and key cities West Coast Development Region. Gokgyo stream adjacent to the cities plays an important role as a drainage channel for an agriculture and a discharger of urban storm water. Waster quality of the stream has been deteriorating caused by pollution sources such as a untreated wastewater discharge and runoff from the watershed. In the study, we conducted 4 surveys in April, May, July, and September to understand the current state of water quality for the stream and to make it possibe to predict future water-quality variation for future development. The system runs on a personal computer under the windows enviroment and provides extensive graphic user interface(GUI) for user-friendly assessment. Using the pull-down menus provided by the GUI panel, the user is able to operate the system by pointing and clicking the icon to identify the state of water-quality at locations concerned. Furthermore, we developed an integrated watershed management system. The constructed system could be a useful tool as a decesion maker for pollution source control strategy.

• BMB Reports
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• v.45 no.9
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• pp.515-520
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• 2012

High expression of osmotically responsive genes1 (HOS1), a key regulator of low temperature response and flowering time, encodes an E3 ubiquitin ligase in Arabidopsis. Here, we report characterization of a newly identified splice variant (HOS1-L) of HOS1. Comparative analyses revealed that HOS1-L has a longer 5' nucleotide sequence than that of the previously identified HOS1 (HOS1-S) and that its protein sequence was more conserved than that of HOS1-S in plants. HOS1-L transcripts were spatio-temporally more abundant than those of HOS1-S. The recovery rate of HOS1-S expression was faster than that of HOS1-L after cold treatment. Diurnal oscillation patterns of HOS1-L revealed that HOS1-L expression was affected by photoperiod. An in vitro pull-down assay revealed that the HOS1-L protein interacted with the ICE1 protein. HOS1-L overexpression caused delayed flowering in wild-type plants. Collectively, these results suggest regulation of HOS1 expression at the post-transcriptional level.

• BMB Reports
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• v.42 no.11
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• pp.764-768
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• 2009

The tandem ubiquitin-interacting motif (UIM) domain located at the N-terminus of Receptor Associated Protein 80 (RAP80) plays a crucial role in ionizing radiation (IR)-induced DNA damage response. RAP80 translocates to sites of IR-induced DNA damage through interaction of its UIM domain with ubiquitinated H2A and Lys63-linked polyubiquitin chains. The exact mechanism, however, through which RAP80 associates with Lys63-linked polyubiquitin chains is not clear. Here, we show by in vitro GST-pull down assays that modifying the linker region between the tandem ubiquitin binding domains of RAP80 changes the binding affinity for Lys63-linked polyubiquitin chains and affects translocation to sites of DNA breaks. Based on these findings, we suggest that the length of the linker region between the tandem ubiquitin binding domains of RAP80 may be a key factor in the binding of RAP80 with Lys63-linked polyubiquitin chains as well as in the translocation of RAP80 to DNA break sites.

• Korean Journal of Applied Biomechanics
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• v.13 no.3
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• pp.15-26
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• 2003

This study was conducted to investigate the performance times, CM position and CM speed, pole chord length and pole chord angle, whole body angular momentum(X axis), and grip width in pole vault event according to the event and phase; touch down, pole plant, take-off, maximum pole bending pole straight, pole release, peak height, and foot contact, pole contact, free flight. The pole vaulting of four male elite vaulters including six trial were filmed using two video digital cameras at 60 Hz at 56th national athletic match, and data were collected through the DLT method of three dimensional cinematography. In general the better jumper is, the longer the performance time is. And the greater CM speed is, and the better his transformation ability of CM horizontal speed into vertical speed is. As he uses a longer pole, his grip is higher, and it is a enough for him to rock back his body, so that he pulls and pushes the pole well keeping his hips close to. An greater maximum angular momentum and early positioning of the hips parallel to the bar makes his body far side of the bar and his bar clearance easier. Specially our national jumper needs to have more powerful braking force during foot contact phase, and take his body on the pole after maximum pole bending, and pull and push the pole strongly keeping his hips close to. Also he needs to have stronger muscular strength in order to control the longer pole and use the pole of proper tension more efficiently.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2010.07a
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• pp.74-76
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• 2010

Digital images or videos are used in modern digital devices. The resolution of HDTV in digital broadcasting system is higher than that of previous analog systems. Also, mobile phone with 3G can provide images as well as video streaming services in realtime. In these circumstances, the visual quality of images has become an important factor. We can make image clear by transient improvement process that reduces transient in edges. In this paper, we present an transient improvement algorithm. The proposed algorithm improves edges by making smooth edge to steep edge. Before performing transient improvement algorithm, edge detection algorithm should be operated. Laplacian operator is used in edge detection, and the absolute value of it is used to calculate gain value. Then, local maximum and minimum values are computed to discriminate current pixel value to raise up or pull down. Compensating value that gain value multiplies with the difference between maximum (or minimum) value and current pixel value adds (or subtracts) to current pixel value. That is, improved signal is generated by making the narrow transient of edge. The advantage of proposed algorithm is that it doesn't produce shooting problem like overshoot or undershoot.

• Journal of Microbiology and Biotechnology
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• v.16 no.8
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• pp.1285-1291
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• 2006

The proline utilization (put) operon of Vibrio vulnificus consists of the putAP genes encoding a proline dehydrogenase and proline permease. The result of put-lux transcriptional fusion analysis suggests that the V vulnificus putAP operon is not autoregulated by the PutA protein. A putR null mutation decreased proline dehydrogenase activity and the level of the put transcripts, indicating that transcription of putAP is under the positive control of PutR. The deduced amino acid sequence of the putR was similar to those reported from other bacteria with high levels of identity. Chromatin IP and GST pull-down assays revealed that PutR specifically binds to the putAP promoter region in vivo, and interacts with CRP in vitro. Taken together, the results suggested that PutR exerts its effect on putAP expression by directly interacting with CRP bound to the upstream region of P$_{put}$.

• Computers and Concrete
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• v.26 no.3
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• pp.257-273
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• 2020

Concrete bond strength with steel re-bars depends on multiple factors including concrete-steel interface and mechanical properties of concrete. However, the hydration development of cementitious paste, and in turn the mechanical properties of concrete, are negatively affected by cold weather. This study aimed at exploring the concrete-steel bonding behavior in concrete cast and cured under freezing temperatures. Three concrete mixtures were cast and cured at -10 and -20℃. The mixtures were protected using conventional insulation blankets and a hybrid system consisting of insulation blankets and phase change materials. The mixtures comprised General Use cement, fly ash (20%), nano-silica (6%) and calcium nitrate-nitrite as a cold weather admixture system. The mixtures were tested in terms of internal temperature, compressive, tensile strengths, and modulus of elasticity. In addition, the bond strength between concrete and steel re-bars were evaluated by a pull-out test, while the quality of the interface between concrete and steel was assessed by thermal and microscopy studies. In addition, the internal heat evolution and force-slip relationship were modeled based on energy conservation and stress-strain relationships, respectively using three-dimensional (3D) finite-element software. The results showed the reliability of the proposed models to accurately predict concrete heat evolution as well as bond strength relative to experimental data. The hybrid protection system and nano-modified concrete mixtures produced good quality concrete-steel interface with adequate bond strength, without need for heating operations before casting and during curing under freezing temperatures down to -20℃.

• Journal of Microbiology and Biotechnology
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• v.12 no.2
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• pp.286-291
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• 2002

Histone deacetylase I (HDAC1) works as one of the components in a nucleosome remodeling (NuRD) complex that consists of several proteins, including metastasis-associated protein 1 (MTA1). Since the protein-protein interaction of HDAC1 and MTA1 would appear to be important for both the integrity and functionality of the HDAC1 complex, the interruption of the HDAC1 and MTA1 interaction may be an efficient way to regulate the biological function of the HDAC1 complex. Based on this idea, a yeast two-hybrid system was constructed with HDAC1 and MTA1 expressing vectors in the DNA binding and activation domains, respectively. To verify the efficiency of the assay system, 3,500 microbial metabolite libraries were tested using the paper disc method, and KB0699 was found to inhibit the HDAC1 and MTA1 interaction without any toxicity to the wild-type yeast. Furthermore, KB0699 blocked the interaction of HDAC1 and MTA1 in an in vitro GST pull down assay and induced morphological changes in B16/BL6 melanoma cells, indicating the interruption of the HDAC1 complex function. Accordingly, these results demonstrated that the yeast assay strain developed in this study could be a valuable tool for the isolation of a HDAC1 complex disruptor.