• Title/Summary/Keyword: protoplast fusion. carotenoid formation

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Fusion Hybrid and Carotenoid Formation from the Yeast, Phaffia rhodozyma (효모 Phaffia rhodozyma의 융합체와 Carotenoid 생성)

  • Chang, Kee-Myung;Kim, Moon-Hee;Song, Myung-Hee;Kim, Sang-Moon;Chun, Soon-Bai
    • The Korean Journal of Mycology
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    • v.21 no.1
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    • pp.9-15
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    • 1993
  • ABSTRACT: The cell fusants were constructed from complementary auxotrophic mutants of Phffia rhodozyma. The nuclear fusion of the fusants was demonstrated by several techniques including comparision of cell volume, estimation of DNA content and nuclear staining. The cell fusants were very stable for succeeding transfer culture on complex medium for more than one year. Malt extract (1%, w/v) and abscisic acid(1 mM) increased the carotenoid formation whereas gibberellic acid(5mM) and riboflavin(0.1 mM) decreased the corresponding content.

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