• Journal of the Korean Society of Clothing and Textiles
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• v.17 no.3
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• pp.491-505
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• 1993

The influences of protease on the removal of various protein soils from cotton fabrics were studied. The human epidermal stratum corneum, hemoglobin and casein were used as protein soils. The soiled fabrics were denatured by steaming for 30 min. before washing and laundered using Terg-O-Tometer under washing conditions. The removal efficiency was evaluated by analysis of protein on the fabrics before and after washing by means of copper-Folin method. The relations between the removal and the characteristics of protease were discussed. Also the degradation of protein were examined by microscopy. The seperation of human epidermal stratum corneum after hydrolysis was examined by SDS-PAGE. The results obtained were as follow : 1. The protein from the soiled cotton fabric was removed effectively by adding protease. The removal of protein was increased in proportion to increasing of the enzyme concentration up to a certain point, but it began to decrease above the point. The removal effect was high in the order of casein>human epidermal stratum corneum>hemoglobin. Especially the protein was more effectively removed in ADS solution(pH 9.5) containing enzyme. 2. When protease was used with ADS. the removal of protein was efficiently showed in relatively short time(5~15min.) compared to using ADS only. It is due to the properties of this enzyme that reacts with very short time. 3. Even at low temperature the removal efficiency of enzyme was relatively higher compared with the activity of enzyme. The removal of protein soil was increased up to a maximum near $50^{\circ}C$, and then decreased. 4. The removal of protein by protease was improved with the increase of alkalinity in the pH range from 9.5 to 11.0 but it began to decrease above pH 11.0. 5. According to the increase of mechanical agitation, the removal effect was increased. But the removal efficiency of protease was more effective compared with the agitation in detergency. 6. According to the SDS-PAGE separation and micrograph it was confirmed that the human epidermal corneum was effectively hydrolysed by the enzyme added. So the fragments of protein were removed more efficiently by means of the interfacial reaction of AOS.

• Research in Plant Disease
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• v.23 no.1
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• pp.65-68
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• 2017

Barley yellow mosaic virus (BaYMV), which is transmitted by the root-inhabiting protist Polymyxa graminis, causes a soil-borne disease. In this study, we detected BaYMV from soil using two-step nested polymerase chain reaction (PCR). Specific primers based on a coat protein region of BaYMV segment RNA1 were used in the first round of amplification. Based on the sequenced amplicon, an inner primer was designed for the second round of amplification. A PCR product of 372 bp exhibited 98%-100% nucleotide sequence identity with the coat protein region of BaYMV segment RNA1. In this study, we propose an easy method for the detection of BaYMV from soil, may considerably assist in accurate fungus-transmitted virus diagnosis and subsequent disease forecasting. This is the first report on the detection of BaYMV from soil.

• Journal of Microbiology and Biotechnology
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• v.26 no.2
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• pp.248-254
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• 2016

A soil metagenome contains the genomes of all microbes included in a soil sample, including those that cannot be cultured. In this study, soil metagenome libraries were searched for microbial genes exhibiting lipolytic activity and those involved in potential lipid metabolism that could yield valuable products in microorganisms. One of the subclones derived from the original fosmid clone, pELP120, was selected for further analysis. A subclone spanning a 3.3 kb DNA fragment was found to encode for lipase/esterase and contained an additional partial open reading frame encoding a wax ester synthase (WES) motif. Consequently, both pELP120 and the full length of the gene potentially encoding WES were sequenced. To determine if the wes gene encoded a functioning WES protein that produced wax esters, gas chromatography-mass spectroscopy was conducted using ethyl acetate extract from an Escherichia coli strain that expressed the wes gene and was grown with hexadecanol. The ethyl acetate extract from this E. coli strain did indeed produce wax ester compounds of various carbon-chain lengths. DNA sequence analysis of the full-length gene revealed that the gene cluster may be derived from a member of Proteobacteria, whereas the clone does not contain any clear phylogenetic markers. These results suggest that the wes gene discovered in this study encodes a functional protein in E. coli and produces wax esters through a heterologous expression system.

• Journal of the Korean Society of Clothing and Textiles
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• v.20 no.3
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• pp.550-559
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• 1996

The Effect of protease (subtilisin Carlsberg) on the removal of hemoglobin as protein soil was studied. The hydrolysis characteristics of subtilisin Carlsberg was examined by electrophoretic techniques. The fragmentation patterns of hemoglobin were analyzed by SDS-PAGE. The hydrolysis efficiency was evaluated by analysis of protein bands shown on gels before and after hydrolysis by using densitometer. 1. The hydrolysis of hemoglobin by subtilisin Carlsberg was increased markedly with the increase of the enzyme concentration. 2. The hydrolysis of hemoglobin by subtilisin Carlsberg was effectively increased in proportion to increasing of the hemoglobin concentration up to a certain point, but it began to decrease above the point. 3. The hydrolysis of hemoglobin by subtilisin Carlsberg followed the first order kinetics, yielding a rate constant of $4.05\time10^{-4}S^{-1}s$. 4. The hydrolysis of hemoglobin by subtilisin Carlsberg was highest at $50^{\circ}C$ and was decreased markedly at $80^{\circ}C$. 5. The hydrolysis of hemoglobin was comparatively low at pH 7.0~8.0, and highest at pH 11.0.

• Journal of The Korean Society of Grassland and Forage Science
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• v.42 no.3
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• pp.137-145
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• 2022

Aluminum (Al) is one of the major factors adversely affects crop growth and productivity in acidic soils. In this study, the effect of Al on plants in soil was investigated by comparing the protein expression profiles of alfalfa roots exposed to Al stress treatment. Two-week-old alfalfa seedlings were exposed to Al stress treatment at pH 4.0. Total protein was extracted from alfalfa root tissue and analyzed by two-dimensional gel electrophoresis combined with MALDI-TOF/TOF mass spectrometry. A total of 45 proteins differentially expressed in Al stress-treated alfalfa root tissues were identified, of which 28 were up-regulated and 17 were down-regulated. Of the differentially expressed proteins, 7 representative proteins were further confirmed for transcript accumulation by RT-PCR analysis. The identified proteins were involved in several functional categories including disease/defense (24%), energy (22%), protein destination (9%), metabolism (7%), transcription (5%), secondary metabolism (4%), and ambiguous classification (29%). The identification of key candidate genes induced by Al in alfalfa roots will be useful to elucidate the molecular mechanisms of Al stress tolerance in alfalfa plants.

• Journal of The Korean Society of Grassland and Forage Science
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• v.19 no.4
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• pp.373-378
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• 1999

This study was conducted to investigate the effects of application of the dolomite particles and the shell powder on soil characteristics, dry matter yield and nutritive value of forage in sandy loam soil at the Experimental Field of National Livestock Research Institute, Suweon, from 1994 to 1996. This was compared with control, lime, dolomite 0.5mm, 2.0mm, 4.0mm, and shell powder in mixed pasture. Although there was no significant difference, average dry matter yield for 3 years was increased with the application of lime, dolomite 2.0mm, 0.5mm, control, 4.0mm and shell powder in order. Average crude protein yield of forages was increased with the application of dolomite 2.0mm, 0.5mm, control, lime, shell powder and 4.0mm in order. Lime requirement was slightly increased from 2,410 to 4,853kg per ha with the lapse of time. Although soil hardness was optimum level by second year, it was apt to become hard little by little. Solid phase of soil was lowered with dolomite 0.5mm treatment. The results demonstrated that dolomite and shell powder could be settled a dust problem in farms and a coast pollution as lime substitutes for soil improvement. Therefore, it is desirable for dry matter yield and crude protein yield to applicate the dolomite 2.0mm every 3 years and the shell powder more frequently in sandy loam soil.

• Korean Journal of Soil Science and Fertilizer
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• v.42 no.spc
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• pp.33-39
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• 2009

The practice of supplying nutrients for paddy soil with sustaining human health and ecological soundness is to utilize indicators considering soil chemical reactions. The long-term basis experiment of fertilizer and amendment of paddy soil and an experiment of yield response of soil types on nitrogen level from 2000 till 2002were used to search indicators of nutrient supplying capacity related to soil chemical reactions. Chemical reactions of paddy soil was composed of dissociating and/or adsorbing nutrients and of decomposing soil organic matter (SOM) into $H^+$, $e^-$, $CO_2$ in paddy soil. The indicators of nutrient supplying capacity, which were established by considering soil chemical reactions, were SOM or soil protein for nitrogen and available phosphate for phosphorus and cation exchangeable capacity (CEC) and exchangeable potassium for potassium. Korea has used fertilizer recommendation equations established with the indicators of nutrient supplying capacity for paddy soil.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.1
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• pp.7-11
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• 2016

Winter crops have been recognized as an alternative to soil management for fertility and crop productivity in paddy soil. Recently, rape has been produced at winter season and there is little research results on reduction of N fertilizer by adding rape residues for rice cultivation. In this study, we investigated the productivity and quality of rice by applying with 0, 27, 63, $90kg\;ha^{-1}$ of N fertilizer after input of rape residues into soil for two years. The highest yield of rice was average $4.68Mg\;ha^{-1}$ at the treatment applied with the $90kg\;N\;ha^{-1}$ with rape residue. It was reduced to N rate from 31.6 to $43.2kg\;N\;ha^{-1}$ in comparison to $4.53Mg\;ha^{-1}$ of maximum yield in treatment added $90kg\;N\;ha^{-1}$ with rape. Amylose content in rice was similar among treatments with/without rape residue, but protein content was lower in treatment with rape residue than in those without rape residue. In conclusion, input of rape residue in rice cultivation could be alternative to reduction of N fertilization and improvement of quality by adjusting rice productivity in paddy soil under cropping system.

• Journal of Animal Environmental Science
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• v.5 no.1
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• pp.63-72
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• 1999

This study was carried out to improve utilization of fly ash. Each animal waste was mixed with fly ash and composted This compost used at forage crops with corn, rye and alfalfa to examine to examine the fertilized efficiency and investigated productivity of forage crops, composition of this copmost and effect of fly ash on soil characteristics and composition. Content of organic matte, P2O5, K2O, CaO, MgO, Mn and B at the soil, which is given fly ash, increased. After the test crops were harvested, pH of the soil was maintained about 7 and contents of organic matter, phosphoric aicd, K, Mg, and B was increased at the soil of used fly ash. As fly ash was mixed, each DM yield of corn and rye was increased 10∼13% and 14∼21% especially alfalfa was increased 35% at the soil which is mixed fly ash with cage layer manure. As fly ash was mixed, each Crude protein (CP) of corn and rye was increased 6∼17% and about 29%, especially, as fly and cage layer manure was mixed CP of alfalfa was increased 33%. In conclusion, as fly ash is mixed with anlmal waste and use at forage crops, It makes the soil good and improve the productivity of forage crops.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.409-413
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• 2015

To enhance phytoremediation, which removes heavy metal from soil, transgenic plants were applied to contaminated soil. We constructed a transformation vector expressing both $TgMTP_1$ (T. goesingense metal tolerance protein):HA and TgMTP:GFP genes. Transgenic plants were generated using an Agrobacterium-mediated transformation system that expressed the two vectors. Screening and analysis confirmed the incorporation of foreign genes into the Arabidopsis thaliana genome. Callus was induced in the 116 T3 line. These transgenic plants and calli were used for further analyses on the accumulation of Ni. The 116 T3-line plants and calli from selected lines were resistant to heavy metals and accumulated Ni in their leaves. The expression level of TgMTP RNA was equal in all leaves, but protein stability increased in the leaves with Ni treatment. According to these results, we suggest that $TgMTP_1$-overexpressing plants may be useful for phytoremediation of soil.