• 한국식품과학회지
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• 제25권5호
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• pp.475-480
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• 1993

본 연구는 육류등급제 시행에 따라 유화형 소시지 제조시 원료육의 일반성분과 기능성 규명을 통해서 최소사격배합에 입력자료로서 사용될 원료육의 결착지수를 국내 실정에 맞도록 추정하고자 실시하였다. 각 원료육의 일반성분, 기능성 및 육색소 함량은 축종별 부위별로 다양한 수치를 보였으며 원료육의 결착력(cohesiveness)과 높은 상관산계를 갖는 것이 많았다. 특히 수분, 단백질함량과는 정의 상관관계를, 지방함량과는 부의 상관관계를 나타냄으로써 단지 일반성분 분석치만으로도 원료육의 결착성 추정을 위한 회귀식 산출이 가능하였다. 따라서 분석기기가 갖추어지지 않은 공장현장에서도 최소가격배합에 필요한 결착지수의 추정이 가능하며 기존의 결착지수가 밝혀지지 않은 원료육의 결착지수 추정도 가능하였다.

• 한국식품저장유통학회지
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• 제10권2호
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• pp.213-218
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• 2003

재래식 된장의 품질을 고급화하기 위해 재래식 된장과 각종 한약제와 약초류를 달리 첨가하여 제조한 두 종류의 약용 된장들을 6개월간 숙성시키면서 성인병에 관련된 몇 가지 생리 기능성의 변화를 조사하였다 약용 된장들의 고혈압을 예방하는 엔지오텐신 전환효소(ACE) 저해활성은 숙성기간이 길어짐에 따라 급격히 감소하여 숙성 6개월 후에 39.0%∼51.7%을 보였고 혈전용해활성은 숙성 6개월후에 10.4∼11.3 U를 보였다. 약용 된장들의 전자공여능은 제조직후 95%∼97%을 보였고 숙성 기간이 길어짐에 따라 큰 변화가 없었으며 SOD 유사활성은 숙성 6개월 후에 모두 10% 내외를 보였다. 약용 된장(I)의 tyrosinase 저해활성은 숙성 기간이 길어짐에 따라 증가하여 숙성 6개월 후에 상온물 추출물에서 97.6%의 높은 활성을 보였다. 6개월 숙성된약용 된장들의 조단백질과 총당 함량은 각각 14%와 33%로 재래식 된장보다 높았다.

• Bulletin of the Korean Chemical Society
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• 제26권12호
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• pp.1911-1920
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• 2005

Effective artificial metalloproteases have been designed by using cross-linked polystyrene as the backbone. Artificial active sites comprising Cu(II) complexes as the catalytic site and other metal centers or organic functionalities as binding sites were synthesized. The activity of Cu(II) centers for peptide hydrolysis was greatly enhanced on attachment to polystyrene. By placing binding sites in proximity to the catalytic centers, the ability to hydrolyze a variety of protein substrates at selected cleavage sites was improved. Thus far, the most advanced immobile artificial proteases have been obtained by attaching the aldehyde group in proximity to the Cu(II) complex of cyclen.

• Preventive Nutrition and Food Science
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• 제5권4호
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• pp.200-204
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• 2000

Protein conjugation of curdlan belonging to $\beta$-1, 3-glucan was carried out to improve it surface functionalities. The glucan was mixed with phosvitin, {TEX}$$\alpha$_{s}${/TEX}-casein, lysozyme or ovalbumin, respectively. The mixture was freeze-dried, and he resulting powder was incubated at 6$0^{\circ}C$ and 79% relative humidity for 12 days in order to generate a controlled Maillard reaction between curdlan and proteins. conjugation with unfolding proteins, i.e., phosvitin and {TEX}$$\alpha$_{s}${/TEX}-casein, drastically increased the solubility of the glucan, whereas lysozyme and ovalbmin did not. The solubility in water of curdlan was 3.44% for the phosvitin conjugate and 1.09% for the {TEX}$$\alpha$_{s}${/TEX}-casein conjugate. SDS-slab polyacrylamide gel electrophoresis showed that curdlan was solubilized due to covalent binding with phosvitin. Emulsifying properties of curdlan were substantially improved by the conjugation with phosvitin and {TEX}$$\alpha$_{s}${/TEX}-casein. Emulsion stability of the curdlan-phosvitin conjugate was about 2.9 times greater than that of the curdlan-phosvitin mixture.

• Applied Biological Chemistry
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• 제42권2호
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• pp.128-133
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• 1999

참치내장 중 유문수 조직에 내재되어 있는 단백질 분해효소를 부분정제하여 추출한 후 민태 frame 단백질의 효소적 가수분해에 이용하였으며, 제조된 가수분해물을 한외여과막을 이용하여 분자량 크기에 따라 분획한 후 기능성을 개선시킬 목적으로 cyclic sodium trimetaphosphate를 이용하여 인산화 민태 frame 단백질 가수분해 물을 제조하고, 용해도, 유화성 및 포말성 등과 같은 기능성을 검토하였다. 1 가수분해물의 기능성 개선을 목적으로 가수분해물에 STMP를 처리하여 인산화된 가수분해물을 제조하였으며, IR 스펙트럼으로 인산화를 확인한 결과, $1300\;cm^{-1}$에서 P=O의 흡수띠를, $1000{\sim}1100\;^{-1}$에서 P-O-C와 alkyl group에 결합된 P-O-C의 흡수띠를 확인하였다. 2. 민태 frame 단백질 가수분해물을 인산화시킴으로써 용해도는 물론 유화성 및 유화 안정성 그리고 포말 안정성 등 기능성의 개선에 크게 효과를 나타내었다. 특히 분자량이 가장 큰 획분인 P-30K 가수분해물은 모든 기능성 부분에서 좋은 결과를 나타내어 미이용자원으로부터 기능성이 부여된 새로운 가치의 물질로서 활용이 기대된다.

• BMB Reports
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• 제41권3호
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• pp.184-193
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• 2008

Living organisms are comprised of various systems at different levels, i.e., organs, tissues, and cells. Each system carries out its diverse functions in response to environmental and genetic perturbations, by utilizing biological networks, in which nodal components, such as, DNA, mRNAs, proteins, and metabolites, closely interact with each other. Systems biology investigates such systems by producing comprehensive global data that represent different levels of biological information, i.e., at the DNA, mRNA, protein, or metabolite levels, and by integrating this data into network models that generate coherent hypotheses for given biological situations. This review presents a systems biology framework, called the 'Integrative Proteomics Data Analysis Pipeline' (IPDAP), which generates mechanistic hypotheses from network models reconstructed by integrating diverse types of proteomic data generated by mass spectrometry-based proteomic analyses. The devised framework includes a serial set of computational and network analysis tools. Here, we demonstrate its functionalities by applying these tools to several conceptual examples.

• KSBB Journal
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• 제32권3호
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• pp.233-237
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• 2017

Melanin is one of the most important factors affecting skin color. Melanogenesis is the bioprocess of melanin production by melanocytes in the skin and hair follicles and is mediated by several enzymes, such as tyrosinase, tyrosinase related protein (TRP)-1, and TRP-2, MITF. In this study, we investigated the effect of Artemisia fukudo Makino extracts on tyrosinase activity and melanin production as natural products of whitening functional cosmetics. Melanin content in murine B16F10 melanoma cells were decreased by Artemisia fukudo Makino extracts in a dose-dependently. In addition, the inhibition of tyrosinase activity of Artemisia fukudo Makino extracts showed to decrease tyrosinase activity as the concentration of ${\alpha}-MSH$ was increased. Furthermore, western blot analysis revealed that Artemisia fukudo Makino extracts significantly downregulated the expression of tyrosinase, TRP-1 which treat of ${\alpha}-MSH-induced$ melanogenesis in murine B16F10 melanoma cells. As a result, Artemisia fukudo Makino extract showed functionalities as an effective whitening agent to inhibit melanin formation.

• Biomolecules & Therapeutics
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• 제26권3호
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• pp.255-267
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• 2018

Inflammation is one of the main causes of pathologic pain. Knowledge of the molecular links between inflammatory signals and pain-mediating neuronal signals is essential for understanding the mechanisms behind pain exacerbation. Some inflammatory mediators directly modulate the excitability of pain-mediating neurons by contacting the receptor molecules expressed in those neurons. For decades, many discoveries have accumulated regarding intraneuronal signals from receptor activation through electrical depolarization for bradykinin, a major inflammatory mediator that is able to both excite and sensitize pain-mediating nociceptor neurons. Here, we focus on the final effectors of depolarization, the neuronal ion channels, whose functionalities are specifically affected by bradykinin stimulation. Particular G-protein coupled signaling cascades specialized for each specific depolarizer ion channels are summarized. Some of these ion channels not only serve as downstream effectors but also play critical roles in relaying specific pain modalities such as thermal or mechanical pain. Accordingly, specific pain phenotypes altered by bradykinin stimulation are also discussed. Some members of the effector ion channels are both activated and sensitized by bradykinin-induced neuronal signaling, while others only sensitized or inhibited, which are also introduced. The present overview of the effect of bradykinin on nociceptor neuronal excitability at the molecular level may contribute to better understanding of an important aspect of inflammatory pain and help future design of further research on the components involved and pain modulating strategies.

• Food Science and Biotechnology
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• 제15권3호
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• pp.418-423
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• 2006

Egg white powders (EWPs) were produced after pH adjustment (PH 6-9) of fresh egg white, followed by spray-drying, and foaming and gelling properties of EWPs were examined. EWP produced after pH adjustment to 6.5 (EWP-6.5) resulted in significantly higher foaming ability and gel hardness than control and other pH-adjusted EWP. Significant increases in surface -SH content and surface hydrophobicity of EWP-6.5 coincided with improved foaming ability and gel hardness. Significantly higher consistency index for reconstituted EWP-6.5 indicates unfolding of egg white protein was substantially increased in EWP-6.5. Decreased a-helix content in EWP-6.5 was confirmed by circular dichroism spectral analysis. These results indicate pH adjustment prior to spray-drying leads to structural changes in egg white proteins, significantly affecting major functionalities of EWP.

• 방사선산업학회지
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• 제10권4호
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• pp.243-248
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• 2016

Argonaute 2 (AtAGO2) is a well characterized effector protein in Arabidopsis for its functionalities associated with DNA double-strand break (DSB)-induced small RNAs (diRNAs) and for its inducible expression upon ${\gamma}$-irradiation. However, its transcriptional regulation depending on the recovery time after the irradiation and on the specific response to DSBs has been poorly understood. We analyzed the 1,313 bp promoter sequence of the AtAGO2 gene ($1.3kb_{pro}$) to characterize the transcriptional regulation of AtAGO2 at various recovery times after ${\gamma}$-irradiation. A stable transformant harboring $1.3kb_{pro}$ fused with GUS gene showed that the AtAGO2 is highly expressed in response to ${\gamma}$-irradiation, after which the expression of the gene is gradually decreased until 5 days of DNA damage recovery. We also confirm that the AtAGO2 expression patterns are similar to that of ${\gamma}$-irradiation after the treatments of radiomimetic genotoxins (bleomycin and zeocin). However, methyl methanesulfonate and mitomycin C, which are associated with the inhibition of DNA replication, do not induce the expression of the AtAGO2, suggesting that the expression of the AtAGO2 is closely related with DNA DSBs rather than DNA replication.