Applied Biological Chemistry
- Volume 42 Issue 2
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- Pages.128-133
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- 1999
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Improvement of the Functionalities by Phosphorylation of Hoki(Johnius belengeri) Frame Protein Hydrolysates
민태(Johnius belengeri) frame 단백질 가수분해물의 인산화에 의한 기능성 개선
- Jeon, You-Jin (Dept. of Chemistry, Pukyong National University) ;
- Lee, Byoung-Jo (Dongwon Industry Co. LTD.) ;
- Park, Pyo-Jam (Dept. of Chemistry, Pukyong National University) ;
- Byun, Hee-Guk (Dept. of Chemistry, Pukyong National University) ;
- Kim, Se-Kwon (Dept. of Chemistry, Pukyong National University)
- Published : 1999.05.31
Abstract
To enhance functional properties of 4 different hoki frame protein hydrolysates (30K, 10K, 5K and 1K hydrolysate) fractionated through a series of 30, 10, 5 and 1 kDa molecular weight cut-off membranes in order to decrease pore size, all hydrolysates were phosphorylated with sodium trimetaphosphate and altered phosphorylated 30K, 10K, 5K and 1K (P-30K, P-10K, P-5K and P-1K), respectively. The covalent attachment of anionic phosphate groups to polypeptide chains improved the functional properties, such as solubility, emulsifying properties and foaming properties, of hoki frame protein hydrolysates. Especially, P-30K hydrolysate with the highest molecular weight fraction possessed the most excellent functional properties among 4 different phosphorylated hydrolysates.
참치내장 중 유문수 조직에 내재되어 있는 단백질 분해효소를 부분정제하여 추출한 후 민태 frame 단백질의 효소적 가수분해에 이용하였으며, 제조된 가수분해물을 한외여과막을 이용하여 분자량 크기에 따라 분획한 후 기능성을 개선시킬 목적으로 cyclic sodium trimetaphosphate를 이용하여 인산화 민태 frame 단백질 가수분해 물을 제조하고, 용해도, 유화성 및 포말성 등과 같은 기능성을 검토하였다. 1 가수분해물의 기능성 개선을 목적으로 가수분해물에 STMP를 처리하여 인산화된 가수분해물을 제조하였으며, IR 스펙트럼으로 인산화를 확인한 결과,