• Korean Journal of Fisheries and Aquatic Sciences
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• v.2 no.1
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• pp.71-82
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• 1969

The present investigations were carried out with the purpose of making clear the fundamental features of the seasonal variations in chemical composition of the brown algae. Three species of samples, Ecklonia cava, Sargassum sagamianum and Hizikia fusiforme have been analyzed monthly for their contents of total ash, crude protein, alginic acid, mannitol, and laminarin over a year period. Three kinds of samples were collected from the same locality, situated on the southern coast of the Che-ju Island, from September 1966 to August 1967. In addition, the comparative analysis was made on fronds and stipes of the plant for their chemical composition. The results obtained are summarized as follows: 1, In general, the three species examined underwent a similar mode of seasonal variation, and no essential difference was detected among them. 2. The chemical composition of the plant exhibited a considerable difference between the species. The content of total ash in H. fusiforme was remarkably higher than those in the two other species respectively. The alginic acid content was relatively high in S. sagamianum and low in H. fusiforme. The contents of crude protein, mannitol and laminarin were appreciably high in E. cava and low in H. fusiforme in general. 3. The most outstanding feature in the seasonal variation was that, in general, the total ash, crude protein, and alginic acid contents were at a maximum in the winter months while laminarin and mannitol contents were at a minimum. The converse was true in summer. Total ash-Maximum values were observed from December to February and minimum from August till October. Crude Protein-All species exhibited maxima in January and February, and minima from August to October. Alginic acid-Maximum contents occured from January to March and minimum from September to November. Mannitol-The maximum content of mannitol was In May and lune and minimum in January, February and March. Leminerin-Maximum content was in September and October, and minimum in January and February. 4. The wide seasonal variation in chemical constitution occured in the fronds, but the stipes showed a slight seasonal variation. In the chemical composition, the stipe was high in alginic acid, low in mannitol and laminarin. The reverse was in the frond.

• Applied Biological Chemistry
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• v.23 no.1
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• pp.14-22
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• 1980

White and black sesame produced in Korea were defatted with ethyl ether or n-hexane. Defatted sesame meal was extracted with water and salt solution, and protein extraction was precipitated at various pH 1 through 12, with trichloro acetic acid (TCA), tannic acid and ammonium sulfate, respectively. Protein was purified by Sephadex A-25, G-75, G-100 and G-200, and identified its protein fraction by polyacrylamide gel electrophoresis. Amino acids composition of protein in white sesame was analyzed by automatic amino acid analyzer. Protein contents of white sesame, black sesame and sesame meal are 20.5%, 19.2%, and 44.7%, respectively. n-Hexane was the most suitable solvent for extraction of oil from sesame. Crude protein precipitation was better in higher pH. The protein extraction was more effective with the solution containing sodium chloride tinder the pH 8. Globulin in total protein was high and prolamin was less than in other cereal proteins. Glutamic acid contents of white sesame and sesame globulin were 17.1%, and 20%, respectively. Both proteins contained relatively high levels of essential amino acids. 12-13 bands were found in water soluble protein and 2 bands in salt soluble protein were detected by the disc gel electrophoresis, and were identified in both of white and black sesame. The salt soluble protein of white sesame could be purified by Sephadee G-100 and G-200.

• The Journal of the Korea Contents Association
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• v.8 no.6
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• pp.74-81
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• 2008

Protein interaction network contains a small number of highly connected protein, denoted hub and many destitutely connected proteins. Recently, several studies described that a hub protein is more likely to be essential than a non-hub protein. This phenomenon called as a centrality-lethality rule. This nile is widely credited to exhibit the importance of hub proteins in the complex network and the significance of network architecture as well. To confirm whether the rule is accurate, we Investigated all protein interaction DBs of yeast in the public sites such as Uetz, Ito, MIPS, DIP, SGB, and BioGRID. Interestingly, the protein network shows that the rule is correct in lower scale DBs (e.g., Uetz, Ito, and DIP) but is not correct in higher scale DBs (e.g., SGD and BioGRID). We are now analyzing the features of networks obtained from the SGD and BioGRD and comparing those of network from the DIP.

• Journal of the Korean Society of Tobacco Science
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• v.15 no.1
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• pp.63-74
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• 1993

Changes in the amount of ribulose 1, 5-bisphosphate carboxylase/oygenase(=RuBisCO) protein, namely fraction I protein, and the protease activity were determined in the 10th leaf of tobacco(Nicotiana tabacum, var. Ky-57) from 10 days after emergence through senescence at 5 days interval. The amount of RuBisCO per deveined leaf rapidly increased during the early growing season, reached a maximal quantity at the around 20 days after leaf emergence, when the leaf has gone through its most rapid expansion, and began gradually to decrease till 30 days after leaf emergence, thereafter significantly declined to 45 days that the leaf has been dried up partly. The pattern of the ratio of RuBisCO protein to soluble protein in quantity changed similar to that of RuBisCO contents in a leaf, that was 43%, 60%, and 21% at the around 10 days, 20 days, and 45 days, respectively. And RuBisCO contents was linearly correlated with the concentration of chlorophyll(r=0.98) throughout the life span of the leaf. So, it was assumed that the leaf color can be a useful indicator for judging whether RuBisCO contents higher or not in tobacco leaves without homogenization. On the other hand, the protease activities for degradation of casein were assayed at pH 5.5. 7.0. and 8.5 with crude extracts desalted on Sephadex G-25. The highest caseolytic activity was found at pH 5.5 throughout the life sawn of the leaf. Also, the activity at 5.5 became gradually to increase from 30 days after leaf emergence, when RuBisCO protein had became to disappear and remarkably increased in the last stage of senescence, although nitrogen contents of the leaf had reached low levels. The caseolytic activity at pH 5.5 was in negative correlation with RuBisCO contents throughout the life span of the leaf, but not in lineality between them. In other words, the caseolytic activity increased in a rapid exponential manner when RuBisCO contents had reached some low levels. These results showed that the leaf age, namely harvesting time, is a very important factor for the production of the tobacco leaf containing higher RuBisCO protein. It was concluded that the practical harvesting time is between 20 days and 30 days after the leaf emergence from the present results.

• Journal of the Korean Society of Food Science and Nutrition
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• v.13 no.2
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• pp.156-162
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• 1984

The objective of this study was to investigate the influence of three types of protein source in a basal diet during the prenatal and lactating periods on the cellular growth and development of organs in rate. Twenty four pregnant Sprague-Dawley rats weighing 187.7${\pm}$18.5 g were fed ad libitum diets containing casein, soy protein, or corn gluten. The weight of organ and the contents of DAN, RNA, and protein of organs in pups were determined at 0, 3, 7, 14, and 21 days after the birth. Dietary intake of rats during trimester of pregnancy and lactation were significantly higher in the rats fed case in containing diet than those of rats fed the corn gluten diet. Body weight of the progeny fed casein diet at birth and after 7 days were significantly higher than those of rats fed the corn gluten diet. The weight of brain, liver, and kidney of pups which fed corn gluten diet were significantly lower than those fed the casein diet. DNA contents in the brain of pups fed the casein and the soy protein were slightly higher than those fed the corn gluten diet. RNA and protein contents of the brain, and DNA, RNA and protein contents of the liver of the corn gluten treated group were significantly lower than those of the others.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.10a
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• pp.13-13
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• 2019

Soybean (Glycine max L. (Merr) is commonly consumed and found in major foods including soymilk, soy sauce, tofu, and soy sprout in Korea and east Asia. In addition, it is common to cook the whole seeds with rice. Soybean is known to have ranges of health benefits including antiaging, anticancer, neuroprotective and antidiabetic taken either as supplement or dietary food. Anthocyanins and flavonoids in G. max are found to be the main contributors to such wide arrays of health benefits. Due to increasing economic values of soybean, development of specialty soybean cultivars is becoming an area of interest worldwide. In this study, 746 black soybean accessions from National Agrobiodiversity Center were characterized as part of an attempt to identify important germplasms of G. max. Seed coats of each accession were analyzed for their total anthocyanin, cyanidin 3-O-Glucoside (C-3-O-G), delphinidin 3-O-glucoside (D-3-O-G), petunidin-3-O-glucoside (Pt-3-O-G), and their whole seeds for crude protein contents. HPLC was used to determine and quantify the anthocyanin compositions while crude protein was determined using Kjeldahl method by Kjeltec auto-analyzer (Kjeltec 8400, Foss, Sweden). Accessions were grouped according to their anthocyanins and protein contents; the mean content of which were correlated to agronomic traits including maturity date, one hundred seed weight, cotyledon color and seed lust color. The results indicated that the total anthocyanin content (TAC) ranged from 273.77 to 6250.52 mg/100 g, with mean value of 1853.03 mg/100 g while the crude protein content (CPC) being between 33.43 and 47.51%, with mean value of 40.81%. The highest number of accessions (45.97%) showed TAC between 1000~1900 mg/100 g while 30.96% of accessions showed CPC between 41~43%. Among the 746 accessions considered, 11 (IT142935, 175818, 175855, 177191, 177209, 177211, 177214, 177216, 177218, 177220, 177274) of them showed TAC above 4000 mg/100 g. C-3-O-G was found to be the major contributor to TAC showing strong correlation. Accessions with green cotyledon color showed high mean TAC compared to those having yellow cotyledon color, and accessions with dull seed lust color showed high mean TAC than those having shiny seed lust color. One hundred seeds weight and maturity date showed positive correlation with all anthocyanin contents, except for Pt-3-O-G in the latter case. The overall result of the present study could be used as background for developing new black soybean cultivars and breeds with high anthocyanin and protein contents. The result depicted that many of the accessions could be used as potential parental lines.

• Korean Journal of Veterinary Research
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• v.11 no.2
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• pp.145-148
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• 1971

Serum Samples from adult of Korean cattles including 40 females and 20 males were analyzed by sodium salt precipitation and colorimetric method in the purpose of the determination of total serum protein, albumin, globulin, ${\alpha}$-globulin, ${\beta}$-globulin and ${\gamma}$-globulin. The results obtained arc summarized as follows: 1. Mean value of total serum protein showed a slight variation from 7.6%, and its regional and sex differences were not found to be significant. 2. Contents of albumin in serum showed lower level than that of globulin as low level of A/G ratio 0.4 in proportion. 3. Contents of Serum ${\alpha}$-globulin showed 1.4w/v% and $1.51{\pm}0.46$w/v% in each group of female, and $1.31{\pm}0.26$w/v%, in the group of male. 4. Contents of serum ${\beta}$-globulin showed 1.74w/v%, 1.95w/v%, in each group of female, and 1.82w/v% in the group of male. 5. Contents of serum ${\gamma}$-globulin showed 2.32w/v%, 2.30w/v% in each group of female, and 2.30w/v%, in the group of male.

• Journal of the Korean Society of Tobacco Science
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• v.19 no.2
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• pp.77-82
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• 1997

In order to investigate biochemical activities of harvested tobacco leaves, photosynthetic rate, soluble protein contents, and peroxidale activities were analysed during different maturing period. Physiological activities of harvested leaves during maturing period were higher in topped than those of non-topped plants. Chlorophyll content and photosynthetic rate in both topped and non-topped plants decreased at 4 days and 3 days before harvest, respectively. The chloroplast numbers in topped and non-topped plants decreased at 3 days and 5 days before harvest, respectively. Changes of soluble protein and total RNA contents showed similar patterns during maturing period. Soluble protein contents were slightly decreased from 5 days before harvest in topped plants, but decreased drastically from 3 days before harvest in non-topped plants. Not much changes were found in total RNA contents in topped plants until 2 days before harvest, and it was largely decreased after 5 days before harvest in non-topped plants. The peroxidase activities drastically decreased in topped plants and increased in non-topped plants after 3 days before harvest during maturing period. The largest change of biochemical activities in tobacco leaves during maturing periods were observed at 3 days before harvest.

• The Korean Journal of Food And Nutrition
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• v.31 no.3
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• pp.435-440
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• 2018

This study analyzed the general components of rice bread with 75% rice content such as water, crude ash, crude protein, crude fat and carbohydrate, and its nutrients such as vitamin C, vitamin A, vitamin E content, minerals, amino acids, and fatty acids. The contents of crude protein were high and those of crude fat were low with the contents of crude ash, crude protein, and crude fat being 1.61, 18.50, 0.04 g/100 g respectively. Vitamin A was not detected and the contents of vitamin C and vitamin E were 3.85 and 3.04 mg/100 g, respectively. The calcium, potassium, magnesium, iron and sodium contents were 222.0, 117.90, 24.12, 2.30, 555.90 mg/100 g respectively. Rice bread contains 9 essential amino acids such as valine, leucine, isoleucine, methionine, threonine, lysine, phenylalanine, histidine, and arginine. The analysis of rice bread fatty acid showed 58.04 mg/100 g of saturated fatty acid, 26.31mg/100 g of monounsaturated fatty acid and 15.64 mg/100 g of polyunsaturated fatty acid. The total essential fatty acid content was 15.49 mg/100 g. With the rising interest in processed rice products, well-being, and diet, it is necessary to develop processed rice foods that are nutritional and low in calories using rice powder that is nutritionally better than flour.

• Korean Journal of Microbiology
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• v.19 no.2
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• pp.52-62
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• 1981

In order to interpret the effect of IAA on the phosphate metabolism and biosynthesis of organic compounds, Saccharomyces uvarum were cultured in the media treated with various concnetration of IAA $(10^{-3}M,\;10^{-5}M,\;10^{-7}M)$. Sampling at the beginning and intervals of culture, yeast cells fractionated were traced the contents of inorganic phosphate and organic compounds of various fractions. 1. Growth of Saccharomyces uvarum were enhanced by IAA $(10^{-3}M,\;10^{-5}M)$ and phosphate contents in DNA and RNA fractions treated with IAA were accelerated 2.3 times and 2 times in comparison with those of control. 2. Amounts of poly-P"A" and poly-p"B" were increased but poly-P"C" decreased during the culture. Therefore, it is considered that poly-P"C" play on most important role as a phosphate pool. 3. It is suggested that because phosphate contents in DNA, protein and lipid fractions increased, inorganic phosphates required phosphates required RNA were transferred from phosphates in cytoplasm, because these increased slowly during the culture. 4. Alkali-labile protein were accelerated by IAA and alkali stable protein only were inhibiction were enhanced by IAA while, ethanol : ether soluble fraction was induced by $10^{-7}M$ IAA in comparison with those control.X> IAA in comparison with those control.