• The Plant Pathology Journal
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• 제36권5호
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• pp.385-397
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• 2020

The ascomycete fungus Colletotrichum gloeosporioides infects a wide range of plant hosts and causes enormous economic losses in the world. The transcription factors (TFs) play an important role in development and pathogenicity of many organisms. In this study, we found that the C₂H₂ TF CgCrzA is localized in both cytoplasm and nucleus under standard condition, and it translocated from cytoplasm to nucleus in a calcineurin-dependent manner. Moreover, the ΔCgCrzA was hypersensitive to cell wall perturbing agents and showed severe cell wall integrity defects. Deletion of the CgCRZA inhibited the development of invasive structures and lost pathogenicity to plant hosts. Our results suggested that calcineurin-responsive TF CgCrzA was not only involved in regulating cell wall integrity, but also in morphogenesis and virulence in C. gloeosporioides.

• Journal of Microbiology and Biotechnology
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• 제30권7호
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• pp.967-973
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• 2020

The fungal cell wall is a major target of antifungals. In this study, we report the antifungal activity of an ethanol extract from Aucklandia lappa against Candida albicans. We found that the extract caused cell wall injury by decreasing chitin synthesis or assembly and (1,3)-β-D-glucan synthesis. A sorbitol protection assay demonstrated that the minimum inhibitory concentration (MIC) of the A. lappa extract against C. albicans cells increased eight-fold from 0.78 to 6.24 mg/ml in 72 h. Cell aggregates, which indicate damage to the cell wall or membrane, were commonly observed in the A. lappatreated C. albicans cells through microscopic analysis. In addition, the relative fluorescence intensities of the C. albicans cells incubated with the A. lappa extract for 3, 5, and 6 h were 92.1, 84.6, and 79.8%, respectively, compared to the controls, estimated by Calcofluor White binding assay. This result indicates that chitin content was reduced by the A. lappa treatment. Furthermore, synthesis of (1,3)-β-D-glucan polymers was inhibited to 84.3, 79.7, and 70.2% of that of the control treatment following incubation of C. albicans microsomes with the A. lappa extract at a final concentration equal to its MIC, 2× MIC, and 4× MIC, respectively. These findings suggest that the A. lappa ethanol extract may aid the development of a new antifungal to successfully control Candidaassociated disease.

• 한국건축시공학회지
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• 제13권6호
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• pp.513-521
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• 2013

This study aimed to examine whether heat curing methods of concrete subjected to $-10^{\circ}C$ could be effective by varying the combination of heating cable and surface heat insulations. Three different concrete specimens incorporating 30% fly ash with 50% W/B were fabricated to simulate wall, column and slab members with dimensions of $1600{\times}800{\times}200$ mm for slab, $800{\times}600{\times}200$ mm for wall and $800{\times}800{\times}800$ mm for column. For heat curing combinations, Type-1 specimens applied PE film for slab, plywood for wall and column curing. Type-2 specimens applied double layer bubble sheet (2LB) and heating coil for slab, and 50 mm styrofoam for wall and column curing. Type-3 specimen applied 2LB for slab, electrical heating mat for wall and column inside heating enclosure. The test results revealed that the temperature of Type 1 specimen dropped below $0^{\circ}C$ beginning at 48 hours after placement due to its poor heat insulating capability. Type 2 and 3 specimens maintained a temperature of around $5{\sim}10^{\circ}C$ after placement due to favorable heat insulating and thermal resistance.

• The Plant Pathology Journal
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• 제31권1호
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• pp.50-60
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• 2015

The use of novel isolates of Trichoderma with efficient antagonistic capacity against Fusarium oxysporum f. sp. lycopersici (FOL) is a promising alternative strategy to pesticides for tomato wilt management. We evaluated the antagonistic activity of 30 isolates of T. asperellum against 4 different isolates of FOL. The production of extracellular cell wall degrading enzymes of the antagonistic isolates was also measured. The random amplified polymorphic DNA (RAPD) method was applied to assess the genetic variability among the T. asperellum isolates. All of the T. asperellum isolates significantly reduced the mycelial growth of FOL isolates but the amount of growth reduction varied significantly as well. There was a correlation between the antagonistic capacity of T. asperellum isolates towards FOL and their lytic enzyme production. Isolates showing high levels of chitinase and ${\beta}$-1,3-glucanase activities strongly inhibited the growth of FOL isolates. RAPD analysis showed a high level of genetic variation among T. asperellum isolates. The UPGMA dendrogram revealed that T. asperellum isolates could not be grouped by their antagonistic behavior or lytic enzymes production. Six isolates of T. asperellum were highly antagonistic towards FOL and potentially could be used in commercial agriculture to control tomato wilt. Our results are consistent with the conclusion that understanding the genetic variation within Trichoderma isolates and their biochemical capabilities are required for the selection of effective indigenous fungal strains for the use as biocontrol agents.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2002년도 학술대회지
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• pp.229-232
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• 2002

For the purpose of Thermal Protection Material design problem, a numerical analysis of axisymmetric high temperature supersonic impinging jet flows of exhaust gas from combustor on curved surfaces has been accomplished. A modifed CSCM Upwind Navier-Stokes method which is able to cure the carbuncle Phenomena has been developed to study strong shock wave structure and thermodynamic wall properties such as pressure and heat transfer rate on various curved surfaces. The results show that the maximum heat transfer rate which is the most important parameter affecting thermo-chemical surface ablation on the plate did not occur at the center of jet impingement, but rather on a circle slightly away from the center of impingement and the shear stress distribution alone the wall is similar to the wall heat transfer late distribution.

• 복합신소재구조학회 논문집
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• 제1권4호
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• pp.6-12
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• 2010

This study investigates how to apply composite material to the blast loading protection devices, mainly used for military purpose. Traditionally, earth-filled blast walls have been used for protecting important parts of military facilities and personnels. However these types of blast walls show difficulty in fabrication and portability because of their nature of heavy weight. Composite materials are known to have relatively higher specific stiffness and strength than any other metallic and earth-filled materials such as sand and gravels. Totally 4 times of TNT blast experiments were performed on the carbon/epoxy blast walls. After the end of each test, the improvement of blast wall was implemented to the structure. The test results show that the use of composite material in the blast protecting area is the one of very effective and reliable alternatives.

• 대한방사선방어학회:학술대회논문집
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• 대한방사선방어학회 2011년도 추계 학술발표회 및 심포지엄
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• pp.236-237
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• 2011

• 한국환경과학회지
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• 제13권6호
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• pp.505-512
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• 2004

This study was attempted to review the information data for minimizing the destruction of environmental naturalness and the visual damage of landscape from road construction by establishing a model of environmental naturalness for national roads in the suburb areas to suggest an answer to a research question, ' hat does decide the environmental naturalness of roadscape?'. We found that 1) The road-side slope showed no statistical significance in the description of environmental naturalness of roadscape, but the fact that the road-side slope from road construction is the destruction of natural topography cannot be overlooked. 2) In terms of the direction of value variations for independent variables, signboard and telegraph post, soundproofing and protection wall, structure, and building acted toward negative (-) direction, while mountains, sky, road trees, fields, and surrounding green including the road-side slope acted toward positive(+) direction. 3) The variable with highest relative contribution to dependent variables among independent variables is building, which has importance as many as 148 times of road-side slope, while the variable road-side slope has the least importance. Building has the importance of 7.22 times, mountains 5.51 times, road trees 2.59 times, surrounding green 2.54 times, structure 2.41 times, signboard and telegraph post 2.37 times, soundproofing and protection wall 2.20 times, and sky 1.32 times of the fields as a standard criterion values 1.

• 한국조경학회지
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• 제17권2호
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• pp.1-14
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• 1989

The purpose of this study was to develop the principle of space composition in Jungja of a category of korean traditional space. The results which analyzed outside space, prospect angle and preference factors for landscape with making, Jungja which has artificially constructed pond within Gyeongbug province, the subject of study were as fellows ; Size of pond was quite different in each Jungja, and its form was retangular. There was built circle island or figured ten-long life within pond. These pursued harmony of the cosmic dual force or eternal youth. Average angle of depression and horizontal angle from floor of Jungja to pond were respectively 16$^{\circ}$ 23', 48$^{\circ}$ 26'. Average angle of depression to island in pond was 13$^{\circ}$28'. Average angle of depression from floor of Jungia to wall was 2$^{\circ}$58'. With controlling height of wall, there was maintained privacy and used borrowed landscape. Tree within garden was mainly planted a deciduous tall tree, the important species of tree were bamboo, pomegranate tree, crape myrtle, etc. Variables that gave influence on landscape preference degree were things about tree and pond. On prospecting pond, these variables-feeling for shore protection, impression for water, visual depth to pond feeling for shore protection, impression for water, visual depth to pond-gave Largely influences.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2008년도 추계 학술발표회
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• pp.660-671
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• 2008

The suspension bridge between Myodo and Gwangyang is located in the main navigation channel to Gwangyang Harbor. So, there is need for the collision protection against large vessels. As ship collision protection, artificial island with concrete block quay wall is planned. The risk analysis and non-linear numerical analysis are introduced to consider the ship collision effects. In the Gwangyang bay area, there are some different sedimental conditions in clayey stratums. For a desirable design, we classify into four zones and 2 layers in each zone, and then determine suitable soil properties considering these zones. As a ground improvement under artificial island, DCM and SCP methods are Planned.