• Archives of Plastic Surgery
• /
• 제40권6호
• /
• pp.676-686
• /
• 2013

Background To overcome the potential drawbacks of a short half-life and dose-related adverse effects of using active transforming growth factor-beta 1 for cartilage engineering, a cell-mediated latent growth factor activation strategy was developed incorporating latent transforming growth factor-${\beta}$1 (LTGF) into an electrospun poly(L-lactide) scaffold. Methods The electrospun scaffold was surface modified with NH3 plasma and biofunctionalised with LTGF to produce both random and orientated biofunctionalised electrospun scaffolds. Scaffold surface chemical analysis and growth factor bioavailability assays were performed. In vitro biocompatibility and human nasal chondrocyte gene expression with these biofunctionalised electrospun scaffold templates were assessed. In vivo chondrogenic activity and chondrocyte gene expression were evaluated in athymic rats. Results Chemical analysis demonstrated that LTGF anchored to the scaffolds was available for enzymatic, chemical and cell activation. The biofunctionalised scaffolds were non-toxic. Gene expression suggested chondrocyte re-differentiation after 14 days in culture. By 6 weeks, the implanted biofunctionalised scaffolds had induced highly passaged chondrocytes to re-express Col2A1 and produce type II collagen. Conclusions We have demonstrated a proof of concept for cell-mediated activation of anchored growth factors using a novel biofunctionalised scaffold in cartilage engineering. This presents a platform for development of protein delivery systems and for tissue engineering.

• 공업화학
• /
• 제31권5호
• /
• pp.581-584
• /
• 2020

The effect of a chemical pretreatment on the surface carbon was investigated using a scanning electron microscope (SEM) and electrochemical methods. Primitive carbon has a reducing power likely due to incompletely oxidized functional groups on the surface. We aim to control this reducing power by chemical treatment and apply for the spontaneous deposition of nanoparticles (NPs). Highly ordered pyrolytic graphite (HOPG) was initially treated with a reducing agent, NaBH4 or an oxidizing agent, KMnO₄, for 5 min. Subsequently, the pretreated carbon was immersed in a platinum (Pt) precursor. Unexpectedly, SEM images showed that the reducing agent increased spontaneous PtNPs deposition while the oxidizing agent decreased Pt loading more as compared to that of using bare carbon. However, the amount of Pt on the carbon obviously decreased by NaBH₄ treatment for 50 min. Secondly, spontaneous reduction on pretreated glassy carbon (GC) was investigated using the catalytic hydrogen evolution reaction (HER). GC electrode treated with NaBH4 for a short and long time showed small (onset potential: -640 mV vs. MSE) and large overpotential for the HER, respectively. Although the mechanism is unclear, the electrochemistry results correspond to the optical data. As a proof-of-concept, these results demonstrate that chemical treatments can be used to design the shapes and amounts of deposited catalytic metal on carbon by controlling the surface state.

• 한국실내디자인학회논문집
• /
• 제18권6호
• /
• pp.150-157
• /
• 2009

In the 1970's the Interior Design Associations was established and the activity of youth designers who werecalled the "first generation of Korean interior designers" were created. This study is aimed to analyze characteristics and trends of commercial interior design in the 1970's. The design methods include the documentary research and the actual proof research conducted. The frames of analysis were made by the background theories about Korean interior design, and the annual case studies were analyzed and estimated according to the design types. The design types were analyzed by the geometrical simplicity research, the romantic emotional expression, the Korean identity expression, the machine technical asthetic expression and the eclectic style with western classics. In the early 1970's, the abstract, brief, and simple expression were presented most frequently by the geometrical form and the repetition of the pattern. From the mid-1970's the romantic and emotional atmosphere of the youth culture that was popular at that time were expressed as vernacular design by the rough finishing of the natural materials such as plaster, brick, and wood floorings etc. The space such as a Korean food restaurant relates to the Korean traditional culture aims to be different through the expression by the Korean traditional patterns, furniture, and materials. In the late 1970's the metals and glass were used for the expression of the machine aesthetic form but was not popular because of the rare application. The type that revived the past western traditional form was presented by using the arch, dome, and the curved and luxurious moldings.

• 한국전자통신학회논문지
• /
• 제17권6호
• /
• pp.1157-1166
• /
• 2022

비대면 시대의 돌입 후 가상현실 플랫폼인 메타버스(Metaverse)에서 아바타를 활용한 새로운 유형의 디지털 성범죄가 발생하고 있다. 이러한 아바타의 성범죄는 현재, 법률 및 정책적 체계가 명확히 확립되지 않아 범죄자에 대한 처벌과 디지털 증거를 수집하는 방법에 어려움을 겪고 있다. 본 논문에서는 가상현실 공간인 메타버스에서 발생하는 디지털 성범죄를 중심으로 범죄예방을 원활하게 하고 디지털 증거를 수집할 수 있는 서비스·기술적 방법을 플랫폼 구축으로 제안하고자 한다. 연구 방법으로 메타버스에서 법률적 적용이 어려운 이유에 대하여 알아본 뒤, 메타버스 안에서 발생할 수 있는 디지털 성범죄의 형태를 파악하고 아바타의 범죄행위를 예방하기 위해 메타버스 플랫폼에서 어떠한 것을 활용할 수 있는지 제작 및 설계 단계부터 갖추어야 할 서비스 요소는 어떠한 것들이 있는지를 제안한다.

• 융합보안논문지
• /
• 제23권5호
• /
• pp.45-55
• /
• 2023

IT 기술의 발전으로 따른 디지털 기기 사용의 보편화와 함께, 익명 통신 기술의 규모 또한 기하급수적으로 증가하고 있다. 이러한 상황에서 특히, 다크 웹(Dark web)과 딥웹(Deep web) 등 익명성을 보장하는 보안 메신저가 디지털 범죄의 온상지가 되고 있다. 익명 네트워크를 이용한 범죄 행위는 사용 기기에 로컬 데이터를 거의 남기지 않아 행위 추적이 어렵다. 미국 연방형사소송규칙과 영국 수사권한법에서는 온라인 수색 관련 법 및 제도 도입을 통해 대응하고 있으나, 한국은 관련 법의 부재로 인하여 수사적 대응 또한 전무한 실정이다. 종래의 (해외에서 사용되는) 온라인 수색 기법은 프로세스가 종료되면 아티팩트(Artifact) 수집을 할 수 없고, 메모리에만 데이터를 저장하는 악성코드에 대응할 수 없으며, 민감 데이터 식별이 어렵고, 무결성이 침해된다는 기술적 한계가 확인된다. 본 논문에서는 기본권 침해를 최소화하는 방향에서 물리 메모리 데이터 분석을 통한 익명 네트워크 사용자 행위 추적 기반 블록체인 범죄 수사방식의 국내 도입 방안을 제안한다. 클로링을 통해 수집한 다크 웹 사이트 사용자의 행위를 추적해 물리 메모리의 잔존율과 77.2%의 합의 성공률을 확인함으로써 제안 방안의 수사로서의 실효성을 입증하고자 하였다.

• 한국식품과학회지
• /
• 제12권1호
• /
• pp.53-58
• /
• 1980

건조(乾燥) 고추의 변색(變色) 요인(要因)을 구명(究明)키 위(爲)하여 수분(水分) 활성(活性), 광선(光線), 산소(酸素) 및 온도(溫度)를 달리 했을 때의 capsanthin의 소장(消長)과 또 몇가지 구장재(句裝材)로 가루, 원형(原形) 및 원형(原形) 압축(壓縮) 상태(狀態)로 포장(包裝)하여 capsanthin 양(量)과 색도(色度)에 관한 시험(試驗) 결과(結果)는 다음과 같다. 1. 고추의 capsanthin에 미치는 상온하(常溫下)($25^{\circ}C$)의 한계(限界) 수분(水分) 활성(活性)은 0.75이었다. 2. Capsanthin의 감소(減少)는 적외선(赤外線), 백열등(白熱燈) 하(下)에서 보다 자외선(紫外線) 이 심했다. 3. Capsanthin의 질산(窒素) 가스 내(內)에서는 거의 감소(減少)가 없었고 공기(空氣) 내(內)에서 심함 감소(減少)가 있는 것으로 보아 고추의 변색(變色)은 주(主)로 산화(酸化) 작용(作用)에 기인(基因)하는 것으로 본다. 4. $9{\sim}11$월(月)의 상온(常溫)과 저온(低溫) 저장(貯藏) 시(時)에는 포장(包裝)된 고추의 capsanthin의 변화(變化)는 별로 차이(差異)가 없었다. 5. 형태(形態) 별(別) 3개월(個月) 저장(貯藏) 시험(試驗) 결과(結果)는 capsanthin 양(量)과 색도(色度)는 가루 저장(貯藏)이 가장 높았다. 6. 포장(包裝) 재료(材料) 별(別) 밀봉(密封) 저장(貯藏) 시험(試驗) 결과(結果)는 투수성(透水性)과 투기성(透氣性)이 없는 재료(材料)가 P.E.보다 capsanthin 양(量)과 색도(色度)가 높았다.

• 대한두경부종양학회지
• /
• 제13권2호
• /
• pp.206-212
• /
• 1997

H$\"{u}$rthle cell neoplasm of the thyroid gland is an uncommon, but potentially malignant lesion. However, in many instances, the malignant potential of the H$\"{u}$rthle cell neoplasm is very difficult to judge histologically. For this reason, the biologic behavior of this tumor and its optimal treatment have come under considerable debate in recent years. In order to review the clinicopathologic features of the H$\"{u}$rthle cell neoplasm and to determine its optimal treatment modalities, we studied 26 patients with path logical proof of H$\"{u}$rthle cell tumor from January 1987 to September 1997. We also performed an immunohistochemical study using the monoclonal antibodies against antigen CD34 for the angiogenic activity of this tumor and evaluated the differences of microvessel density(MVD) between benign and malignant tumors. The age of the patients ranged from 1 to 71 years with a mean of 44.2 years. There were 6 males and 20 females(M : F= 1 : 3.3). The accuracies of fine needle aspiration biopsy and frozen section were very low; 6.3% and 34.8%, respectively. There were 20 benign tumors and 6 malignant tumors(23.1%). All the malignant tumors were microinvasive(intermediate) type which had minimal capsular invasion and most of them(5 cases) were diagnosed postoperatively. Any specific clinicopathologic differences were not seen between benign and intermediate groups. Most of the cases had conservative surgeries(15 ipsilateral lobectomy-isthmusectomy, 7 subtotal thyroidectomy) while total thyroidectomy was performed in 4 cases. Of the cases with malignant tumor, 2 had ipsilateral lobectomy-isthmusectomy, 3 had subtotal thyroidectomy and the remaining 1 had total thyroidectomy. Mean size of the tumors was 3.0 cm(0.1- 8.5 cm) in the greatest diameter and multiple tumors were seen in 6 cases(23.1 %). During the follow-up period, only one recurrence(3.8%) of benign tumor occurred but distant metastasis or cause-specific death was seen in the benign or intermediate groups. Mean MVDs of the benign(n=13) and intermediate(n=6) groups were $121.7{\pm}35.3$ and $114.3{\pm}31.7$, respectively and there was no statistical significance between them. In conclusion, because of the low accuracies of fine needle aspiration biopsy and frozen section for the H$\"{u}$rthle cell neoplasm, the extent of surgery could be individualized based on permanent pathologic examination; Conservative surgery would be adequate for patients with benign or intermediate H$\"{u}$rthle cell neoplasm and total or near-total thyroidectomy for those with definite malignancy.

• 한국식품저장유통학회지
• /
• 제24권7호
• /
• pp.934-941
• /
• 2017

파프리카는 우리나라의 대표적인 수출작물로 알려져 있지만 장거리 유통 중 일어나는 품질열화로 인해 상품적 가치가 감소되는 문제가 있다. 따라서 본 연구는 농산물 유통 시 주로 사용되는 골판지상자의 내부에 알루미늄을 코팅하여 기능성을 부여한 포장용기를 개발하였으며, 이를 이용하여 파프리카를 포장하여 저장 중 선도유지 및 품질특성에 미치는 영향을 알아보았다. 골판지상자 내부에 알루미늄을 코팅함으로써 방습성을 향상시키고 기체투과도를 저하시킴으로써 포장된 파프리카의 펙틴분해효소 활성이 저하됨에 따라 파프리카의 중량변화율과 조직연화 현상이 저해되었고, 산화효소 활성이 감소함으로써 총 페놀 함량 및 항산화 활성이 향상되는 것으로 나타났다. 결과적으로 알루미늄 코팅한 골판지상자는 파프리카의 상품성 및 저장성을 향상시키는 포장용기로 이용될 수 있을 것으로 생각되며, 알루미늄박의 내수성, 내한성, 보향성, 내오염성 등의 특성을 활용한 추가연구를 통해 국내 농산물의 장거리 유통 및 수출 활성화에 이바지할 수 있을 것이라 생각된다.

• Nutrition Research and Practice
• /
• 제17권2호
• /
• pp.371-385
• /
• 2023

BACKGROUND/OBJECTIVES: Soy isoflavone (SIF) and soy lecithin (SL) have beneficial effects on many chronic diseases, including neurodegenerative diseases. Regretfully, there is little evidence to show the combined effects of these soy extractives on the impairment of cognition and abnormal cerebral blood flow (CBF). This study examined the optimal combination dose of SIF + SL to provide evidence for improving CBF and protecting cerebrovascular endothelial cells. MATERIALS/METHODS: In vivo study, SIF50 + SL40, SIF50 + SL80 and SIF50 + SL160 groups were obtained. Morris water maze, laser speckle contrast imaging (LSCI), and hematoxylin-eosin staining were used to detect learning and memory impairment, CBF, and damage to the cerebrovascular tissue in rat. The 8-hydroxy-2'-deoxyguanosine (8-OHdG) and the oxidized glutathione (GSSG) were detected. The anti-oxidative damage index of superoxide dismutase (SOD) and glutathione (GSH) in the serum of an animal model was also tested. In vitro study, an immortalized mouse brain endothelial cell line (bEND.3 cells) was used to confirm the cerebrovascular endothelial cell protection of SIF + SL. In this study, 50 µM of Gen were used, while the 25, 50, or 100 µM of SL for different incubation times were selected first. The intracellular levels of 8-OHdG, SOD, GSH, and GSSG were also detected in the cells. RESULTS: In vivo study, SIF + SL could increase the target crossing times significantly and shorten the total swimming distance of rats. The CBF in the rats of the SIF50 + SL40 group and SIF50 + SL160 group was enhanced. Pathological changes, such as attenuation of the endothelium in cerebral vessels were much less in the SIF50 + SL40 group and SIF50 + SL160 group. The 8-OHdG was reduced in the SIF50 + SL40 group. The GSSG showed a significant decrease in all SIF + SL pretreatment groups, but the GSH showed an opposite result. SOD was upregulated by SIF + SL pretreatment. Different combinations of Genistein (Gen)+SL, the secondary proof of health benefits found in vivo study, showed they have effective anti-oxidation and less side reaction on protecting cerebrovascular endothelial cell. SIF50 + SL40 in rats experiment and Gen50 + SL25 in cell test were the optimum joint doses on alleviating cognitive impairment and regulating CBF through protecting cerebrovascular tissue by its antioxidant activity. CONCLUSIONS: SIF+SL could significantly prevent cognitive defect induced by β-Amyloid through regulating CBF. This kind of effect might be attributed to its antioxidant activity on protecting cerebral vessels.

• 한국산학기술학회논문지
• /
• 제15권7호
• /
• pp.4184-4190
• /
• 2014

본 연구의 목적은 요양시설 입소노인의 일반적 특성에 따른 스트레스와 생활행동지수를 파악하고자 한다. 연구대상은 서울시 N구에 거주하는 입소노인과 강원도 횡성군 S면 거주하는 입소노인 110명 대상으로 2013년 5월 1일부터 9월30일까지 설문조사를 실시하였다. 자료 분석은 SPSS/WIN 18.0 프로그램을 이용하여 t-test, 상관분석, 다중회귀분석방법 등을 실시하였고, 실증분석은 유의수준 5%에서 검증하였다. 분석결과는 첫째, 입소 증후군은 일상적 스트레스의 가족왕래부족 스트레스와 정의 상관이 있었다. 둘째, 우울증상은 일상적 스트레스의 개인시공간부족, 가족왕래부족 스트레스와 정의 상관이 있었다. 셋째, 체중조절은 일상적 스트레스의 개인시공간부족 스트레스와만 정의 상관이 있었으며, 영양결핍증상은 일상적 스트레스의 가족왕래부족 스트레스와만 정의 상관이 있었다. 본 연구의 시사점은 입소노인의 일반적 특성에 따른 스트레스와 생활행동지수 정책의 기초자료가 될 것으로 사료된다.