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검색결과 394건 처리시간 0.025초

한우 보증씨수소 집단의 유전적 다양성 및 구조 변화 분석 (Analysis of Genetic Diversity and Structural Changes in Hanwoo Proven Bulls Population)

  • 신동현;김도현;오재돈
    • 동물자원연구
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    • 제29권4호
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    • pp.142-149
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    • 2018
  • 본 연구는 한우 보증씨수소 844두를 출생년도를 기준으로 8개 집단으로 분류하고, 각 개체들의 친자확인용 유전자 마커정보를 농협경제지주 한우개량사업소 홈페이지에서 제공 받아 유전적 다양성 및 구조 변화 분석에 활용하였다. 한우 보증씨수소 전체 집단의 대립유전자수(number of alleles)의 평균은 10.54개, 기대 및 관측 이형접합율($H_{ex}$, $H_{ob}$)의 평균은 각각 0.764, 0.773, 다형성 정보량 지수(PIC)의 평균은 0.727 그리고 $F_{is}$의 평균은 -0.014로 확인되었다. 한우 보증씨수소 집단을 출생년도 별로 구분한 8개 집단의 유전적 다양성 및 구조 분석 결과, D집단(2005-2004년)의 기대이형접합율(0.777), 관측이형접합율(0.792) 그리고 다형성정보지수(0.740)가 가장 높은 것으로 확인되었다. C집단(2003-2004년)과 E집단(2007-2008년)에서는 기대이형접합율이 관측이형접합율 보다 큰 것으로 확인되었고, 나머지 그룹 모두에서는 관측이형접합율이 기대이형접합율 보다 큰 것으로 확인되었다. 대립유전자 출현빈도를 기반으로 유전적 조성과 구조를 추론하기 위해 STRUCTURE software를 이용하여 분석한 결과 세대가 지남에 따라 특정 유전적 성분의 변화 또는 비중의 증감을 확인 할 수 있었다. 이는 개량 목표를 설정하고 지속적으로 추진되고 있는 개량 사업이 한우 씨수소 집단의 유전적 구조 변화에 영향을 미치고 있음을 확인 할 수 있는 중요한 자료로, 한우 개량 사업의 효율적인 추진을 위해 유용하게 활용 될 것으로 사료된다.

우리나라 대학생의 음주행태 심층조사 (College Alcohol Study for Alcohol-Related Behaviors and Problems)

  • 주영준;오소연;박상익;이혜자;유민규;박은철
    • 보건행정학회지
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    • 제29권1호
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    • pp.58-67
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    • 2019
  • Background: In this study, we aimed to investigate the drinking behaviors and drinking-related problems of college students in South Korea to produce national alcohol statistics. Methods: We carefully examined the questionnaires and previous research developed in the previous research project and selected questions that reflect the special environment and culture of college students. In order to stratify a nationally representative sample of college students, the distribution of students around the country were found through the educational statistics database of the Korea Educational Development Institute. Based on this information, we conducted a survey in collaboration with Gallup (Korea) to survey and analyze the drinking behaviors of 5,024 Korean students. Results: A nationwide cross-sectional survey was conducted in 2017, for Korean college students. A total of 5,024 students were recruited and analyzed. The monthly drinking rate was 78.0% for male students and 72.9% for female students. The high-risk drinking rate was 23.3% for male students and 17.2% for female students. The most popular category for number of drinks per drinking session was 'more than 10 glasses' per drinking session for both male (44.1%) and female (32.8%). On the alcohol use disorders identification test, the greatest proportion of male students were in the high-risk drinking category (score 8 to 15) 43.8%, followed by the 'low-risk drinking' (score 0 to 7) in 43.6%, 'alcohol abuse' (score 16 to 19) 7.2%, and 'alcohol dependence' (greater than 20) 5.4% categories, respectively. For female students, the greatest proportion of female students were in the 'low-risk drinking' in 49.6%, followed by 'high-risk drinking' 37.1%, 'alcohol abuse' 8.4%, and 'alcohol dependence' 4.9% categories, respectively. Conclusion: The results of the study showed that the drinking behavior of Korean college students was excessive. Overall, it was found that the college population has a greater high-risk drinking behaviors than general adult population. Furthermore, these problem drinking behaviors were prominent among female college students. Results from the present study suggest that it is necessary to monitor the drinking behavior of college students with constant interest and to prepare policies and strategies suitable for these circumstances.

Adjunctive hyperbaric oxygen therapy for irradiated rat calvarial defects

  • An, Heesuk;Lee, Jung-Tae;Oh, Seo-Eun;Park, Kyeong-mee;Hu, Kyung-Seok;Kim, Sungtae;Chung, Moon-Kyu
    • Journal of Periodontal and Implant Science
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    • 제49권1호
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    • pp.2-13
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    • 2019
  • Purpose: The aim of this study was to conduct a histologic evaluation of irradiated calvarial defects in rats 4 weeks after applying fibroblast growth factor-2 (FGF-2) with hyaluronan or biphasic calcium phosphate (BCP) block in the presence or absence of adjunctive hyperbaric oxygen (HBO) therapy. Methods: Twenty rats were divided into HBO and non-HBO (NHBO) groups, each of which was divided into FGF-2 and BCP-block subgroups according to the grafted material. Localized radiation with a single 12-Gy dose was applied to the calvaria of rats to simulate radiotherapy. Four weeks after applying this radiation, 2 symmetrical circular defects with a diameter of 6 mm were created in the parietal bones of each animal. The right-side defect was filled with the materials mentioned above and the left-side defect was not filled (as a control). All defects were covered with a resorbable barrier membrane. During 4 weeks of healing, 1 hour of HBO therapy was applied to the rats in the HBO groups 5 times a week. The rats were then killed, and the calvarial specimens were harvested for radiographic and histologic analyses. Results: New bone formation was greatest in the FGF-2 subgroup, and improvement was not found in the BCP subgroup. HBO seemed to have a minimal effect on new bone formation. There was tendency for more angiogenesis in the HBO groups than the NHBO groups, but the group with HBO and FGF-2 did not show significantly better outcomes than the HBO-only group or the NHBO group with FGF-2. Conclusions: HBO exerted beneficial effects on angiogenesis in calvarial defects of irradiated rats over a 4-week healing period, but it appeared to have minimal effects on bone regeneration. FGF-2 seemed to enhance new bone formation and angiogenesis, but its efficacy appeared to be reduced when HBO was applied.

BIM 기반 위험요소 도출을 통한 정량적 위험성 평가 모델 개발 - 떨어짐 사고를 중심으로 - (Development of A Quantitative Risk Assessment Model by BIM-based Risk Factor Extraction - Focusing on Falling Accidents -)

  • 고휘재;현지훈;이주희;안요섭
    • 한국건설관리학회논문집
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    • 제23권4호
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    • pp.15-25
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    • 2022
  • 건설산업의 중대재해 발생률 및 사망률이 가장 높아짐에 따라 이를 감축하기 위해 국내에서도 다양한 노력을 기울이고 있다. 그 중 위험성 평가는 시공단계 위험요소를 평가하고 재해 감소대책을 위한 자료로 활용되고 있다. 그러나 기존의 위험성 평가는 수행자의 주관이 개입되며 국내 건설현장에 취약한 측면이 있다. 본 연구는 위험성 평가 분야에 BIM을 활용하여 정량적으로 위험요소를 도출함으로써 리스크를 조기 식별하고 사전제거하는 것을 목표로 위험성 평가를 위한 DB 분류체계를 구축하였으며 BIM을 활용한 위험성 평가의 방법론을 제시한다. 이를 통해 리스크의 사전 제거로 시공 작업자의 안전성을 증대시키고 안전관리 분야의 추가비용을 절감한다. 또한, 신규 공법에도 적용 가능하므로 프로젝트 참여자들의 이해도를 높이며 의사소통의 도구가 된다. 본 연구는 BIM을 기반으로 정량적인 위험도를 도출하는 프레임워크를 제안하는 연구로써, 향후 BIM을 활용한 위험성 평가 분야에 기반 기술로 활용될 것이다.

국가전거공동활용시스템의 활용 및 발전 방안에 관한 연구 (A Study on the Utilization and Development of the Korean National Authority Data Sharing System)

  • 백지원;이성숙
    • 한국비블리아학회지
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    • 제34권1호
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    • pp.121-143
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    • 2023
  • 본 연구는 국가지식정보자원의 효과적인 구축과 활용을 위해 국립중앙도서관을 중심으로 한 국가 전거데이터 구축 사업의 현황을 파악하고, 국가전거의 국제표준식별체계와의 연계 가능성 및 개선사항을 밝히고, 향후 발전 방안을 제시하는 것을 목적으로 하여 수행되었다. 이를 위해 이 연구는 국가전거공동활용시스템 가입 기관을 대상으로 면담 및 설문조사를 실시하였다. 조사는 전거 관련 현황과 어려움, 국가전거공동활용시스템의 활용, 식별자에 대한 인식 및 활용, ISNI와의 연계에 대한 인식 및 활용, 국가전거공동활용시스템 활성화 요인에 대한 중요도 및 달성도, 전반적인 만족도 및 개선 방향에 대한 항목으로 구성되었고, 이러한 조사 연구를 통해 국가전거공동활용시스템을 통한 국가 전거데이터 구축과 활용의 현황 및 문제점을 파악하고, 국가 전거데이터와 국제 표준 식별 시스템의 연계 가능성을 분석하였으며, 중요도-성과 분석을 통해 개선 사항의 우선순위를 제시하였다. 분석의 결과, 향후 국가 전거 구축의 발전 방안으로는 국가전거공동활용시스템의 활성화를 위해 전거 관련 규칙과 지침 개발, 전거데이터의 양적 확보 및 품질 제고, 전거 업무의 중요성 인식 제고와 효율적인 업무 체계 마련, 전거 서비스의 다양화와 활용 모델 개발, 국가전거공동활용시스템과 국제표준 식별자의 연계, 국가전거공동활용시스템의 필요성 공유 등이 제안되었다.

Introduction of Child and Adolescent Mental Health Services in Korea and Their Role During the COVID-19 Pandemic: Focusing on the Ministry of Education Policy

  • Seo Jung Kim;Jongha Lee
    • Journal of the Korean Academy of Child and Adolescent Psychiatry
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    • 제34권1호
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    • pp.4-14
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    • 2023
  • This study aimed to discuss mental health services for children and adolescents that are being implemented as initiatives of the Korean government and to review the functions and roles of these projects during the COVID-19 pandemic. Three government departments are in charge of providing mental health services for children and adolescents: Ministry of Education, Ministry of Gender Equality and Family, and Ministry of Health and Welfare. The Ministry of Education has implemented several policies to facilitate the early detection of mental health issues among school students (from preventive interventions to selective interventions for high-risk students). The Ministry of Gender Equality and Family additionally serves out-of-school children and adolescents by facilitating early identification of adolescents in crises and providing temporary protection or emergency assistance (as required) through the Community Youth Safety-Net Project. Furthermore, the Ministry of Health and Welfare operates relevant mental health agencies for individuals of all ages including children and adolescents. Any high-risk students who have been screened through the projects of the Ministry of Education are supported through referrals to the following institutions for appropriate treatment of their symptoms: specialized hospitals, the Youth Counseling and Welfare Center operated by the Ministry of Gender Equality and Family, the National Youth Healing Center, the Mental Health Welfare Center operated by the Ministry of Health and Welfare, the Suicide Prevention Center, and the Child Welfare Center. To assist students who are facing any psychological difficulties because of the COVID-19 pandemic, the Ministry of Education has established a psychiatric support group for providing emergency mental health care; furthermore, schools are promoting psychological surveillance (e.g., provision of non-face-to-face counseling services that are centered around the Wee Center). The Ministry of Education, Ministry of Gender Equality and Family, and Ministry of Health and Welfare have provided varied mental health support services in order to address the challenges faced by children and adolescents during the pandemic. Nevertheless, the mental health services operated by each ministry do show some limitations because their service provision system is insufficiently collaborative. The present study discussed the positive effects of each initiative as well as its limitations; furthermore, it suggested improvements for facilitating the healthy development of children and adolescents' mental health.

APPLICATION OF WIFI-BASED INDOOR LOCATION MONITORING SYSTEM FOR LABOR TRACKING IN CONSTRUCTION SITE - A CASE STUDY in Guangzhou MTR

  • Sunkyu Woo;Seongsu Jeong;Esmond Mok;Linyuan Xia;Muwook Pyeon;Joon Heo
    • 국제학술발표논문집
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    • The 3th International Conference on Construction Engineering and Project Management
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    • pp.869-875
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    • 2009
  • Safety is a big issue in the construction sites. For safe and secure management, tracking locations of construction resources such as labors, materials, machineries, vehicles and so on is important. The materials, machineries and vehicles could be controlled by computer, whereas the movement of labors does not have fixed pattern. So, the location and movement of labors need to be monitored continuously for safety. In general, Global Positioning System(GPS) is an opt solution to obtain the location information in outside environments. But it cannot be used for indoor locations as it requires a clear Line-Of-Sight(LOS) to satellites Therefore, indoor location monitoring system could be a convenient alternative for environments such as tunnel and indoor building construction sites. This paper presents a case study to investigate feasibility of Wi-Fi based indoor location monitoring system in construction site. The system is developed by using fingerprint map of gathering Received Signal Strength Indication(RSSI) from each Access Point(AP). The signal information is gathered by Radio Frequency Identification (RFID) tags, which are attached on a helmet of labors to track their locations, and is sent to server computer. Experiments were conducted in a shield tunnel construction site at Guangzhou, China. This study consists of three phases as follows: First, we have a tracking test in entrance area of tunnel construction site. This experiment was performed to find the effective geometry of APs installation. The geometry of APs installation was changed for finding effective locations, and the experiment was performed using one and more tags. Second, APs were separated into two groups, and they were connected with LAN cable in tunnel construction site. The purpose of this experiment was to check the validity of group separating strategy. One group was installed around the entrance and the other one was installed inside the tunnel. Finally, we installed the system inner area of tunnel, boring machine area, and checked the performance with varying conditions (the presence of obstacles such as train, worker, and so on). Accuracy of this study was calculated from the data, which was collected at some known points. Experimental results showed that WiFi-based indoor location system has a level of accuracy of a few meters in tunnel construction site. From the results, it is inferred that the location tracking system can track the approximate location of labors in the construction site. It is able to alert the labors when they are closer to dangerous zones like poisonous region or cave-in..

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미생물법의학: 차세대염기서열분석 방법에 따른 MLVA 결과 비교 및 이를 활용한 DNA 감식 (Microbial Forensics: Comparison of MLVA Results According to NGS Methods, and Forensic DNA Analysis Using MLVA)

  • 윤형석;이승호;임승현;이대상;구세훈;김정은;정주환;김성주;허경행;송동현
    • 한국군사과학기술학회지
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    • 제27권4호
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    • pp.507-515
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    • 2024
  • Microbial forensics is a scientific discipline for analyzing evidence related to biological crimes by identifying the origin of microorganisms. Multiple locus variable number tandem repeat analysis(MLVA) is one of the microbiological analysis methods used to specify subtypes within a species based on the number of tandem repeat in the genome, and advances in next generation sequencing(NGS) technology have enabled in silico anlysis of full-length whole genome sequences. In this paper, we analyzed unknown samples provided by Robert Koch Institute(RKI) through The United Nations Secretary-General's Mechanism(UNSGM)'s external quality assessment exercise(EQAE) project, which we officially participated in 2023. We confirmed that the 3 unknown samples were B. anthracis through nucleic acid isolation and genetic sequence analysis studies. MLVA results on 32 loci of B. anthracis were analysed by using genome sequences obtained from NGS(NextSeq and MinION) and Sanger sequencing. The MLVA typing using short-reads based NGS platform(NextSeq) showed a high probability of causing assembly error when a size of the tandem repeats was grater than 200 bp, while long-reads based NGS platform(MinION) showed higher accuracy than NextSeq, although insertion and deletion was observed. We also showed hybrid assembly can correct most indel error caused by MinION. Based on the MLVA results, genetic identification was performed compared to the 2,975 published MLVA databases of B. anthracis, and MLVA results of 10 strains were identical with 3 unkonwn samples. As a result of whole genome alignment of the 10 strains and 3 unknown samples, all samples were identified as B. anthracis strain A4564 which is associated with injectional anthrax isolates in heroin users.

한국의 유전적 정보 생산 구조 (The Production Structure of Genetic Information in South Korea)

  • 이정호
    • 과학기술학연구
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    • 제5권1호
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    • pp.55-92
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    • 2005
  • 한국에서 중요한 과학적 개념의 형성과 그 사회적 유통에 기여하는 것은 미국과 유럽, 그리고 일본에서 형성, 성숙, 정립된 후에 한국으로 유입되고 수용되는 과학 지식과 한국 사회가 근대화의 과정에 형성한 제도이다. 유전적 정보라는 개념도 이러한 맥락에서 예외일 수는 없다. 유전적 정보 개념은 고전적 유전학의 틀에서 이해되는 유전, 또는 계승성의 개념에서 인간유전체 연구사업의 와중에서 등장하여 성숙한 유전체학과 생물정보학에 의해 확대 심화된 것이다. 본 연구는 서구적 개념 및 지식 생산 구조를 모델로 하는 개념적, 과학지식적, 제도적 통합성을 기준으로 한국에서 유전적 정보의 생산 구조가 어떻게 형성되어 있는가에 대한 것이다. 한국에서 1980년대 중반에 나타났던 유전공학 담론은 한국에서 분자생물학의 발달은 촉진시켰지만, 생화학-생화학교실과 같은 균형성이 없이 유전공학-유전학교실의 불균형성이 존재하게되었다. 주로 의과대학의 (인간)유전학과 혹은 유전학교실의 수와 질에 있어서의 부족함 때문에 생명과학 전체에 미치는 영향력에서도 크게 성공하지 못했고, 통합적, 거시적 발전을 이루지 못한 것으로 보인다. 유전학의 발전적 재구성이라고 할 수 있는 유전체학은 한국에서는 유럽, 미국, 일본의 인간유전체연구사업의 발전 궤적의 '기초단계' 혹은 '제 1기' 형태에는 거의 인프라, 투자, 연구개발이 없었고 기능유전체학과 단백체학을 중심으로 하는 '성숙단계' 혹은 '제 2기' 형태를 주축으로 하여 한국의 연구개발이 진행되고 있다. 유전체학과 같이 발달한 생물정보학에는 내적 구조에 이미 정보학과 연결되는 논리와 내용을 가지고 있는데, 한국에서는 정보기술(IT)의 아류 정도로 보는 편협하고 왜곡된 시각이 주도적인 가운데 시작된 것으로 보인다. 결과적으로 한국 생물정보학은 유전학 및 생명과학과의 통합적인 면에서는 결함을 노출하고 있다. 이러한 유전적 정보의 생산 구조가 가지는 문제점으로 인하여 한국에서 유전적 정보는 기초가 부실한 편이며 파편화된 유형으로 생산되어 나올 개연성을 가진다. 개념적, 제도적인 파편화의 사례는 개인식별의 유전학이 기존의 유전공학-유전학교실 체제로 흡수되지 못하고 의과대학 법의학교실에서 전문성과 연구실천을 확보한 것에서도 확인된다. 유전적 정보의 생산 구조에 영향을 미치는 환경은 한국의 생명공학과 시민사회운동으로 존재한다.

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가정간호사업 운용을 위한 정보전달체계 개발 I (가정간호 데이터베이스 구축과 뇌졸중 환자의 가정간호 전산개발) (Development of the Information Delivery System for the Home Nursing Service)

  • 박정호;김매자;홍경자;한경자;박성애;윤순녕;이인숙;조현;방경숙
    • 가정∙방문간호학회지
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    • 제4권
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    • pp.5-22
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    • 1997
  • The purpose of the study was to development an information delivery system for the home nursing service, to demonstrate and to evaluate the efficiency of it. The period of research conduct was from September 1996 to August 31, 1997. At the 1st stage to achieve the purpose, Firstly Assessment tool for the patients with cerebral vascular disease who have the first priority of HNS among the patients with various health problems at home was developed through literature review. Secondly, after identification of patient nursing problem by the home care nurse with the assessment tool, the patient's classification system developed by Park (1988) that was 128 nursing activities under 6 categories was used to identify the home care nurse's activities of the patient with CAV at home. The research team had several workshops with 5 clinical nurse experts to refine it. At last 110 nursing activities under 11 categories for the patients with CVA were derived. At the second stage, algorithms were developed to connect 110 nursing activities with the patient nursing problems identified by assessment tool. The computerizing process of the algorithms is as follows: These algorithms are realized with the computer program by use of the software engineering technique. The development is made by the prototyping method, which is the requirement analysis of the software specifications. The basic features of the usability, compatibility, adaptability and maintainability are taken into consideration. Particular emphasis is given to the efficient construction of the database. To enhance the database efficiency and to establish the structural cohesion, the data field is categorized with the weight of relevance to the particular disease. This approach permits the easy adaptability when numerous diseases are applied in the future. In paralleled with this, the expandability and maintainability is stressed through out the program development, which leads to the modular concept. However since the disease to be applied is increased in number as the project progress and since they are interrelated and coupled each other, the expand ability as well as maintainability should be considered with a big priority. Furthermore, since the system is to be synthesized with other medical systems in the future, these properties are very important. The prototype developed in this project is to be evaluated through the stage of system testing. There are various evaluation metrics such as cohesion, coupling and adaptability so on. But unfortunately, direct measurement of these metrics are very difficult, and accordingly, analytical and quantitative evaluations are almost impossible. Therefore, instead of the analytical evaluation, the experimental evaluation is to be applied through the test run by various users. This system testing will provide the viewpoint analysis of the user's level, and the detail and additional requirement specifications arising from user's real situation will be feedback into the system modeling. Also. the degree of freedom of the input and output will be improved, and the hardware limitation will be investigated. Upon the refining, the prototype system will be used as a design template. and will be used to develop the more extensive system. In detail. the relevant modules will be developed for the various diseases, and the module will be integrated by the macroscopic design process focusing on the inter modularity, generality of the database. and compatibility with other systems. The Home care Evaluation System is comprised of three main modules of : (1) General information on a patient, (2) General health status of a patient, and (3) Cerebrovascular disease patient. The general health status module has five sub modules of physical measurement, vitality, nursing, pharmaceutical description and emotional/cognition ability. The CVA patient module is divided into ten sub modules such as subjective sense, consciousness, memory and language pattern so on. The typical sub modules are described in appendix 3.

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