• Title/Summary/Keyword: program similarity

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Identification of genes expressed in abalone tissues(Haliotis discus hannai) using expressed sequence tags

  • Nam, Yoon-Kwon;Lee, Sang-Jun;Kim, Koung-Kil;Park, Ji-Eun;Kim, Dong-Soo
    • Proceedings of the Korean Aquaculture Society Conference
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    • 2003.10a
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    • pp.44-44
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    • 2003
  • Gene expression in five tissues of the abalone (Haliotis discus hannai) was investigated using an expressed sequence tag (EST) analysis. Randomly selected clones were obtained from cDNA libraries constructed with gill (GI), digestive diverticula(DD), hepatopancreas (HP), foot/mucus (FM) and rectangular muscle (RM). Of 1,235 clonesanalyzed (288 clones for GI, DD, HP each,166 for FM, and 205 for RM), 741 (60.0%) clones in total turned out to share significant similarity with the sequences from NCBI GenBank (less than 10/sup -3/ of e-values), 423 sequences showed poor similarity (> 10/sup -3/), and 71 sequences didn't match with any sequences in GenBank. The percent unique sequence (singleton) was ranged from 56.1% (RM) to 74.7% (FM) among libraries. On the other hand, overall percent singleton was 55.3% when all the ESTs from five libraries were assembled into contigs. Analysis of the organisms represented by the best hit for each EST (e-values < 10/sup -3/) showed that 23.8% matched with mammalian entries, 24.0% with mollusks, 14.4% with insects, 11.6% with fish and 26.2% with others. The expressed patterns differed among the tissues when judged by the categorization of the sequences from each library into 10 broad functional classes. In all the libraries, the class I (no hit o. poor similarity) was the largest category with an average of 40.1%. This largest class was followed by class V (general metabolisms) in DD (21.9%), GI (14.6%) and HP (16.7%), while the 'cell structure and motility'(class VI) was the second largest class in remaining two libraries (31.2% for RM and 9.6% for FM). The class IX (cell division and proliferation) was the smallest class in all the libraries (less than 3%). This report provides the first tissue-specific lists of expressed abalone genes, which could be a fundamental basis for genomics program of abalone species.

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In silico characterisation, homology modelling and structure-based functional annotation of blunt snout bream (Megalobrama amblycephala) Hsp70 and Hsc70 proteins

  • Tran, Ngoc Tuan;Jakovlic, Ivan;Wang, Wei-Min
    • Journal of Animal Science and Technology
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    • v.57 no.12
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    • pp.44.1-44.9
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    • 2015
  • Background: Heat shock proteins play an important role in protection from stress stimuli and metabolic insults in almost all organisms. Methods: In this study, computational tools were used to deeply analyse the physicochemical characteristics and, using homology modelling, reliably predict the tertiary structure of the blunt snout bream (Ma-) Hsp70 and Hsc70 proteins. Derived three-dimensional models were then used to predict the function of the proteins. Results: Previously published predictions regarding the protein length, molecular weight, theoretical isoelectric point and total number of positive and negative residues were corroborated. Among the new findings are: the extinction coefficient (33725/33350 and 35090/34840 - Ma-Hsp70/ Ma-Hsc70, respectively), instability index (33.68/35.56 - both stable), aliphatic index (83.44/80.23 - both very stable), half-life estimates (both relatively stable), grand average of hydropathicity (-0.431/-0.473 - both hydrophilic) and amino acid composition (alanine-lysine-glycine/glycine-lysine-aspartic acid were the most abundant, no disulphide bonds, the N-terminal of both proteins was methionine). Homology modelling was performed by SWISS-MODEL program and the proposed model was evaluated as highly reliable based on PROCHECK's Ramachandran plot, ERRAT, PROVE, Verify 3D, ProQ and ProSA analyses. Conclusions: The research revealed a high structural similarity to Hsp70 and Hsc70 proteins from several taxonomically distant animal species, corroborating a remarkably high level of evolutionary conservation among the members of this protein family. Functional annotation based on structural similarity provides a reliable additional indirect evidence for a high level of functional conservation of these two genes/proteins in blunt snout bream, but it is not sensitive enough to functionally distinguish the two isoforms.

Identification of Species and Sex of Korean Roe Deer (Capreolus pygargus tianschanicus) Using SRY and CYTB Genes

  • Han, Sang-Hyun;Cho, In-Cheol;Lee, Sung-Soo;Tandang, Leoncia;Lee, Hang;Oh, Hong-Shik;Kim, Byoung-Soo;Oh, Moon-You
    • Animal cells and systems
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    • v.11 no.2
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    • pp.165-168
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    • 2007
  • The nucleotide sequences of a male-specific marker sex determining region Y (SRY) gene and a mitochondrial cytochrome B (CYTB) gene were characterized and analyzed to establish a molecular method for identification of species and sex of Korean roe deer (Capreolus pygargus tianschanicus). Similarity search result of SRY sequences showed very similar result to those reported in Moose (Alces alces) and Reindeer (Rangifer tarandus), both of which had 95.9% similarity in identity. CYTB genes were very similar to those reported in Siberian roe deer (C. pygargus pygargus) which had 98.6% similarity and not to European roe deer (C. capreolus), suggesting that the DNA samples tested were of Siberian roe deer lineage. Polymerase chain reaction (PCR)-based sex typing successfully discriminated between carcasses of male and female roe deer. Males had SRY band on agarose gels and females did not. The result of this molecular sex typing provided similar information with that obtained by genital organ observation. Therefore, this molecular method using male specific marker SRY and mitochondrial CYTB genes would be very useful for identification of the species and sex of the carcass remains of roe deer.

Function partitioning methods for malware variant similarity comparison (변종 악성코드 유사도 비교를 위한 코드영역의 함수 분할 방법)

  • Park, Chan-Kyu;Kim, Hyong-Shik;Lee, Tae Jin;Ryou, Jae-Cheol
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.25 no.2
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    • pp.321-330
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    • 2015
  • There have been found many modified malwares which could avoid detection simply by replacing a sequence of characters or a part of code. Since the existing anti-virus program performs signature-based analysis, it is difficult to detect a malware which is slightly different from the well-known malware. This paper suggests a method of detecting modified malwares by extending a hash-value based code comparison. We generated hash values for individual functions and individual code blocks as well as the whole code, and thus use those values to find whether a pair of codes are similar in a certain degree. We also eliminated some numeric data such as constant and address before generating hash values to avoid incorrectness incurred from them. We found that the suggested method could effectively find inherent similarity between original malware and its derived ones.

The Development of the User-Customizable Favorites-based Smart Phone UX/UI Using Tap Pattern Similarity (탭 패턴 유사도를 이용한 사용자 맞춤형 즐겨찾기 스마트 폰 UX/UI개발)

  • Kim, Yeongbin;Kwak, Moon-Sang;Kim, Euhee
    • Journal of the Korea Society of Computer and Information
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    • v.19 no.8
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    • pp.95-106
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    • 2014
  • In this paper, we design a smart phone UX/UI and a tap pattern recognition algorithm that can recognize tap patterns from a tapping user's fingers on the screen, and implement an application that provides user-customizable smart phones's services from the tap patterns. A user can generate a pattern by tapping the input pad several times and register it by using a smart phone's favorite program. More specifically, when the user inputs a tap pattern on the input pad, the proposed application searches a stored similar tap pattern and can run a service registered on it by measuring tap pattern similarity. Our experimental results show that the proposed method helps to guarantee the higher recognition rate and shorter input time for a variety of tap patterns.

A Study of Symmetry in the Patterns of Muscle Coordination and Interjoint Coordination in the Upper Limb Activity Among Subjects With Stroke (뇌졸중 환자의 상지에서 근육협응 패턴과 관절협응 패턴의 유사성에 관한 연구)

  • Lee, Jung-Ah;Shin, Hwa-Kyung;Chung, Yi-Jung;Cho, Sang-Hyun
    • Physical Therapy Korea
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    • v.13 no.1
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    • pp.54-60
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    • 2006
  • This study aimed to compare movement patterns of shoulder joints between the right and left symmetry in stroke patients and control subjects. This study proposes use of the voluntary response index (VRI) calculated from quantitative analysis of surface electromyographic (sEMG) and motion data recorded during voluntary movement as a feeding task. The VRI is comprised of two numeric values, one derived from the total muscle activity recorded for the voluntary motor task (magnitude), and the other from the sEMG distribution across the recorded muscles with the similarity index (SI). Five stroke patients and five age-matched healthy controls were recruited. Feeding motion was performed using the provided spoon five times with rests taken on a chair in between tasks. EMG data were digitized and analyzed on the basis of the root mean square (RMS) envelope of activity. The average amplitude of responses was calculated. Responsiveness and clinically meaningful levels of discrimination between stroke patients and control for EMG magnitude and SI were determined. The similarity index of the results from two successive examinations of both sides apart for stroke patients and control subjects were .86 and .95 in motion analysis and .84 and .99 in electromyographic analysis. The SI of sEMG data and motion data was significantly correlated in stroke patients. The data suggest that SI is a sensitive program for comparing and analyzing the symmetry of muscle activity and motion in both sides. This analysis method has a clinical value in grading muscular activity and movement impairment after brain injury.

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A comparative study of major of social indicators focusing on the population between Korea and Taiwan (한국과 대만의 주요 사회지표 비교고찰 -인구지표를 중심으로-)

  • 한영자;고갑석
    • Korea journal of population studies
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    • v.9 no.2
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    • pp.30-41
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    • 1986
  • It is worthwhile to compare the major social indicators between Korea the and Taiwan particularly focusing on population because two countries share close similarities in many aspects and gave access to the advanced level among the developing countries in recent years. Similarities or dissimilarities presented in this paper will be helpful to the researchers and the policy makers of the two countries by giving them insight on the situation. The similarities and dissimilarities between the two countries in the field of demography and social indicators are summarized as follows : 1. Similarity indicators can be found in fertility. One of them is Net Reproduction Rate and it was less than one in both countries in 1985. The past trend of fertility, in fact level and pattern of it, of the two countries from 1960 to 1985 has been very similar. 2. Level and pattern of mortality is quite different between two countries. Mortality level of Taiwan is lower than that of Korea. 3. The close similarity between two countries was shown in population structure and aging index. 4. On future population projection, the population structure and the level and pattern of fertility of the two countries are very similar. During fourty years, the period from the current population growth rate which is a little more than 1% to the zero growth rate, the annual population growth rate of the two countries is approximately 0.6% and that is similar to those of the current industrialized countries. According to the medium variant of the projection, Korean population will reach Zero Growth Rate between 2020 and 2025 whereas Taiwan between 2025 and 2020 5. Current level of contraceptive prevalence of both countries is very similar showing above 70% of the eligible women in 1985 and one of the valuable factors of achieving this high rate within short period must be the national program of the family planning. A close cooperation in the field of population policy formulation and its implementation is indispensable because Korea and Taiwan have similarity in many population indicators.

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A Study on the Comparison of 3D Virtual Clothing and Real Clothing by Neckline Type (네크라인 종류에 따른 3D 가상착의와 실제착의 비교 연구)

  • Nam, Young-Ran;Kim, Dong-Eun
    • Fashion & Textile Research Journal
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    • v.23 no.2
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    • pp.247-260
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    • 2021
  • While it is an important element of clothing construction, research has so far been very limited on the similarities between virtual and real clothing in terms of the type of neckline. The purpose of this study is to verify the similarity, accuracy of virtualization, and actuality of neckline, which all play an important role in individual impressions and image formation, and require considerable modification when fitting real samples. A total of 5 neckline models were selected through the analysis of dress composition textbooks. The selected designs were then planned and manufactured in muslin. The specimen clothes were then tested on a female model in her 20s. 2 kinds of virtual bodies were created in order to compare the real and the virtual dressing. The first virtual body was made through an Artec 3D Eva scan of the model, and the other was made by entering the model's measurements in a CLO 3D program. A visual image of the front, side, and back image of both the real and virtual dressing were subsequently collected. The collected images were then evaluated by 20 professional fashion workers who checked the similarity between the real and the virtual versions. The current study found that the similarity between the actual and virtual wearing of the five neckline designs with reality appeared higher with the virtual wearing image using the 3D-scanned body. The results of this study could provide further information on the selection of appropriate avatars to clothing companies that check the fit of clothing by utilizing 3D virtualized programs.

Mechanism Design of Cane-like Passive Type Walking Aid For the Elderly Using 3-RPS Parallel Manipulator (3-RPS 평형기구를 이용한 노인용 지팡이형 보행보조기기 메커니즘 개발)

  • Kim, Jeong-Hun;Jang, Dae-jin;Park, Tae-Wook;Yang, Hyun-Seok;Lee, Sang-Moo
    • Proceedings of the KSME Conference
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    • 2004.11a
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    • pp.725-730
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    • 2004
  • This paper has regarded mechanism design of cane-like passive type walking aid for the elderly using 3-RPS parallel manipulator. First, gait patterns of the elderly have been experimented. By means of motion capturing and image processing, we decided loaded forces and places of the cane when the elderly walked with a cane. Using these results we have developed a passive type walking aid. Second, the walking pattern has been simulated using dynamic analysis program, ADAMS and we find out the similarity between the real walking and the simulated walking. Finally after assuring the similarity, with adjusting the new mechanism design to the simulated walking we will decide whether the walking aid is safe and stable when the elderly walks with this cane-like walking aid. This paper will be basis for the development of the mechanism design applying 3-RPS parallel manipulator.

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A report on 17 unrecorded bacterial species in Korea isolated from Lakes Soyang and Chungju in 2016

  • Jeon, Hyoung Tae;Joung, Yochan;Kim, Suhyun;Lim, Yeonjung;Cho, Jang-Cheon
    • Journal of Species Research
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    • v.6 no.2
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    • pp.163-170
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    • 2017
  • As a part of the research program 'Survey of freshwater organisms and specimen collection', freshwater samples were collected from Lakes Soyang and Chungju in 2016. Hundreds of bacterial strains were isolated from the samples and were identified based on 16S rRNA gene sequences. Among the bacterial isolates, strains showing higher than 98.7% sequence similarity with validly published bacterial species not reported in Korea were selected as unrecorded bacterial species. Based on 16S rRNA gene sequence similarity, 17 strains were identified as unrecorded bacterial species in Korea. The 17 bacterial strains were phylogenetically diverse and belonged to four phyla, seven classes, 13 orders, 14 families, and 16 genera. At generic level, the unreported species were affiliated with Caulobacter, Paracoccus, and Mesorhizobium of the class Alphaproteobacteria, Deefgea, Undibacterium, Chitinimonas, Inhella, and Sphaerotilus of the class Betaproteobacteria, Vibrio and Cellvibrio of the class Gammaproteobacteria, Sanguibacter and Clavibacter of the phylum Actinobacteria, Lactococcus of the phylum Firmicutes, Deinococcus of the class Deinococci, and Chryseobacterium and Flavobacterium of the phylum Bacteroidetes. The unreported species were further characterized by examining Gram reaction, colony and cell morphology, biochemical properties, and phylogenetic position. The detailed description of the 17 unreported species are also provided.