• 미생물학회지
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• 제21권2호
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• pp.51-60
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• 1983

Cultural characteristics of Strptomyces griseolus isolated from the soil were investigated. This strain was disclosed to utilize D-xylose, and D-glactose in preference order as a carbon source with the formation of glucose isomerase. The addition of sweet potato starch also proved effective promoting the total enzyme activity measured at 29% higher than the control. Corn cob, one of waste agricultural resources, was hydrolyzed in 2~3% $H_2SO_4$ solution at $100^{\circ}C$, 3~5 hours to produce a xylose syrup which gave rise to the recovery of 19.9% in a batch system and 28.2% in a repeated system. By the addition of both 2% of xylose syrup(Be'28) prepared by and us 65% of corn steep liquor (total nitrogen 1.2%), enzyme induction was maximized. The enzyme activity was stimulated by the xylose and the cell growth by the C.S.L. Also, remarkable increase of enzyme activity was noticed by the addition of protein acid hydrolysate 86.2% higher than the control. $QO_2$ of the biomass cultured in 30L capacity jarfermentor recorded low oxygen requirement of 251.2 1/hr. Maximum activity of glucose isomerase was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery phase of the biomass growth. Glucose isomerase from the strain was activated by adding the $Co^{++}\;and\;Mg^{++}$ with optimum temperature of $73^{\circ}C$ and pH of 7.2. Conversion ratio of 60% glucose to frutose was 42.5% after 70 hours reaction.

• Journal of the Korean Wood Science and Technology
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• 제51권1호
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• pp.23-37
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• 2023

One type of biomass that has promising potential for bio pellet production is spent coffee grounds (SCGs). However, previous studies have shown that SCGs in bio pellets cause a lot of smoke. Therefore, they need to be mixed with a material that has a higher calorific value to produce better quality pellets. One material that can be used is pine wood because it has a natural resin content that can increase the calorific value. The aim of this study was to examine the quality of bio pellets produced with SCGs and pine wood charcoal at different particle sizes. The charcoal was ground using either a hammer mill (HM) or a ball mill (BM). Pine wood charcoal was mixed with SCGs at ratios of SCGs to pine wood charcoal of 4:6 and 6:4 by weight, respectively, and the adhesive used a tapioca with a composition ratio 5% of the raw material. The bio pellets were produced using a manual pellet press. The quality of the bio pellets was assessed based on Indonesian National Standard (SNI) 8021-2014, and the physical observations include flame length, burning rate, and compressive strength. The average water content, ash content, and calorific value of the bio pellets were in accordance with SNI 8021-2014, but the density and ash content values were below the standard values. The BM variation of bio pellets had a higher compressive strength than the HM variation, and the 4:6 BM variation had the longest burning time compared with 4:6 HM.

• 한국환경농학회지
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• 제28권4호
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• pp.397-402
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• 2009

In Korea, salt stress is one of the major problems limiting crop production and eco-environmental quality in greenhouse soil. The objective of this study was to evaluate the effectiveness of organic residues (Chinese milk vetch, maize stalk, rice straw, and rye straw) for reducing salt activity in greenhouse soil. Organic residues was incorporated with salt-accumulated soil (EC, 3.0 dS $m^{-1}$) at the rate of 5% (wt $wt^{-1}$) and the changes of electrical conductivity (EC) was determined weekly for 8 weeks under incubation condition at $30^{\circ}C$. The EC, microbial biomass carbon (MBC), and water soluble ions in soil was strongly affected by C/N ratio of organic residues. After 8 weeks incubation, the concentration of water soluble $NO_3{^-},\;Ca^{2+}$, and $Mg^{2+}$ was significantly decreased in organic residues having high C/N ratio (maize stalk, rice straw, and rye straw) incorporated soil compared to organic residues having lower C/N ratio (Chinese milk vetch) incorporated soil. The EC value in Chinese milk vetch incorporated soil was higher than control treatment. In contrast, maize stalk, rice straw, and rye straw amended soil was highly decreased the EC value compared to control and Chinese milk vetch applied soil after 4 weeks incubation. Our results indicated that incorporation of organic residues having high C/N ratio (>30) could reduce salt activity resulting from reducing concentration of water soluble ions.

• 한국균학회지
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• 제44권3호
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• pp.155-165
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• 2016

송이산의 방치, 기주식물의 질병, 산불, 기후변화 등 다양한 원인에 의해 국내외 송이 생산량이 감소하고 있어 송이균의 생태적 특성을 이용한 인공재배 기술 개발이 필요한 실정이다. 본 연구에서는 송이 접종묘 생산에 적합한 균주를 선발하기 위해 무균 배양병 내에서 소나무 유묘와 송이균 간의 외생균근 합성을 시도하였다. 송이 균근 형성률은 총 19개 균주 중 5개 균주(NIFoS 421, 434, 1681, 1984, 2001)가 80% 이상을 나타냈다. 7개 송이 균주(NIFoS 434, 441, 561, 562, 1016, 1807, 1812)는 평균 3.0 이하의 S/R ratio를 나타내었고, 소나무 유묘 지상부의 생물량이 지하부보다 2.0~4.8배 정도 높았다. 8개 균주(NIFoS 441, 561, 562, 1016, 1807, 1812, 1984, 2001)는 유묘의 지상부 부피 생장에, 3개 균주(NIFoS 441, 562, 1812)는 균근을 형성하면서 지하부 부피 생장을 돕는 것을 알 수 있었다. 결론적으로 시험균을 접종한 지 6개월 후, 총 19개 시험균 중 4 균주 (NIFoS 434, 561, 1984, 2001)는 타 균주들에 비해 송이균의 균근 형성력과 소나무 유묘 생장력이 우수한 것으로 나타나 송이 접종묘 제작에 있어 이들의 활용이 기대된다.

• 한국미생물·생명공학회지
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• 제24권1호
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• pp.92-100
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• 1996

For the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate)(P(3HB-co-3HV)) from butyric acid and valeric acid, 10 strains of bacteria capable of producing P(3HB-co-3HV) were isolated from soil. Among them, the strain HJ-067 showed the best ability of producing P(3HB-co-3HV), and was indentified as a Azotobacter sp. For the production of P(3HB-co-3HV), the optimum concentrations of butyric and valeric acid were 3.0g/l, respectively. The most effective nitrogen source was $(NH_4)_{2}SO_4$ at an optimum concentration of 0.75g/l, which was equivalent to 21.36 in C/N ratio. Deficiency of the cationic metal ions ($Zn^{2+},\;Co^{2+},\;Mn^{2+}$) in the proguction medium had stimulating effect on P(3HB-co-3HV) accumulation, especially in the manganese. deficient medium. The optimum temperature for P(3HB-co-3HV) production was 27$^{\circ}C$ and the optimum initial pH was 7.0. Under the optimum conditions, 1.82g/l of P(3HB-co-3HV) and 3.00g/l of dry biomass were produced after 36 hour cultivation, and the P(3HB-co-3HV) yield and HV% were 60.60% (w/w), 15.92%, respectively.

• 생태와환경
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• 제37권3호통권108호
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• pp.272-281
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• 2004

팔당호의 유기물 수지 산정을 위해 팔당호내 일차생산력을 측정하고 유입하천의 유입유기물량 및 방류유기물량을 측정하여 유기물부하에서 내부생성유기물의 기여도를 평가하였다. 식물플랑크톤에 의한 일차생산력은 지점과 시기에 따라 큰 차이를 보여 3회 조사결과 $101{\sim}2701\;mgC\;m^{-2}day{-1}$의 범위로 나타났으며, 체류시간이 길고, 수체내 클로로필 a농도가 높았던 6월에 모든 지점에서 높은 생산력을 보였다. 빈번한 강우로 체류시간이 짧고 조류현존량이 적었던 4월과 8월의 조사에서는 총 유기물유입량에서 내부생성유기물량은 약 7%를 차지하였고 이중 식물플랑크톤에 의한 내부생산기여도는 약 5%로 매우 낮았다. 그러나 조사일 전후로 강우가 없어 수체가 안정되었고 외부유입유기물량이 적었던 6월 조사에서는 식물플랑크톤에 의한 내부생산 기여도가 29.0%를 보였다.

• 원예과학기술지
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• 제35권1호
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• pp.21-29
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• 2017

This study was conducted to determine the effects of artificial lighting sources on vegetative growth of Phalaenopsis and Doritaenopsis (an intergeneric hybrid of Doritis and Phalaenopsis) orchids. One - month - old plants were cultivated under fluorescent lamps, cool - white light - emitting diodes (LEDs), or warm - white LEDs at 80 and $160{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. The blue (400 - 500 nm) : green (500 - 600 nm) : red (600 - 700 nm) : far - red (700 - 800 nm) ratios of the fluorescent lamps, cool-white LEDs, and warm-white LEDs were 1 : 1.3 : 0.8 : 0.1, 1 : 1.3 : 0.6 : 0.1, and 1 : 2.7 : 2.3 : 0.4, respectively. Each light treatment was maintained for 16 weeks in a closed plant-production system maintained at $28^{\circ}C$ with a 12 h photoperiod. The longest leaf span, as well as the leaf length and width of the uppermost mature leaf, were observed in plants treated with warm-white LEDs. Plants grown under fluorescent lamps had longer and wider leaves with a greater leaf span than plants grown under cool-white LEDs, while the maximum quantum efficiency of photosystem II was higher under cool-white LEDs. The vegetative responses affected by different lighting sources were similar at both 80 and $160{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$. Leaf span and root biomass were increased by the higher light intensity in both cultivars, while the relative chlorophyll content was decreased. These results indicate that relatively high intensity light can promote vegetative growth of young Phalaenopsis plants, and that warm - white LEDs, which contain a high red-light ratio, are a better lighting source for the growth of these plants than the cool-white LEDs or fluorescent lamps. These results could therefore be useful in the selection of artificial lighting to maximize vegetative growth of Phalaenopsis plants in a closed plant - production system.

• Journal of Microbiology and Biotechnology
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• 제28권10호
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• pp.1671-1682
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• 2018

Alginate lyases (endo and exo-lyases) are required for the degradation of alginate into its constituting monomers. Efficient bioethanol production and extraction of bioactives from brown algae requires intensive use of these enzymes. Nonetheless, there are few commercial alginate lyase preparations, and their costs make them unsuitable for large scale experiments. A recombinant expression protocol has been developed in this study for producing seven endo-lyases and three exo-lyases as soluble and highly active preparations. Saccharification of alginate using 21 different endo/exo-lyase combinations shows that there is complementary enzymatic activity between some of the endo/exo pairs. This is probably due to favorable matching of their substrate biases for the different glycosidic bonds in the alginate molecule. Therefore, selection of enzymes for the best saccharification results for a given biomass should be based on screens comprising both types of lyases. Additionally, different incubation temperatures, enzyme load ratios, and enzyme loading strategies were assessed using the best four enzyme combinations for treating Macrocystis pyrifera biomass. It was shown that $30^{\circ}C$ with a 1:3 endo/exo loading ratio was suitable for all four combinations. Moreover, simultaneous loading of endo-and exo-lyases at the beginning of the reaction allowed maximum alginate saccharification in half the time than when the exo-lyases were added sequentially.

• 한국재료학회지
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• 제22권6호
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• pp.321-327
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• 2012

The production of functional activated carbon materials starting from inexpensive natural precursors using environmentally friendly and economically effective processes has attracted much attention in the areas of material science and technology. In particular, the use of plant biomass to produce functional carbonaceous materials has attracted a great deal of attention in various aspects. In this study the preparation of activated carbon has been attempted from rice husks via a chemical activation-assisted microwave system. The rice husks were milled via attrition milling with aluminum balls, and then carbonized under purified $N_2$. The operational parameters including the activation agents, chemical impregnation weight ratio of the calcined rice husk to KOH (1:1, 1:2 and 1:4), microwave power heating within irradiation time (3-5 min), and the second activation process on the adsorption capability were investigated. Experimental results were investigated using XRD, FT-IR, and SEM. It was found that the BET surface area of activated carbons irrespective of the activation agent resulted in surface area. The activated carbons prepared by microwave heating with an activation process have higher surface area and larger average pore size than those prepared by activation without microwave heating when the ratio with KOH solution was the same. The activation time using microwave heating and the chemical impregnation ratio with KOH solution were varied to determine the optimal method for obtaining high surface area activated carbon (1505 $m^2$/g).

• 환경위생공학
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• 제14권3호
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• pp.130-138
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• 1999

The feasibility of municipal sewage sludge digestion was investigated by using thermophilic anaerobic sequencing batch reactor(ASBR). One-day settle time was enough for the high performance of solid-liquid separation. The conversion of semi-continuous mode to sequencing batch mode is easily achieved without any adverse effects, although the large amount of sludge equal to the volume ratio of 0.3~06 to reactor volume was added in the feed step of the start-up. The ASBRs had higher conversion capability of organics to biogas than the control reactor. Gas yields of the ASBRs were increased by the average of 50% over the control reactor across a range of hydraulic retention time(HRT)s from 10days to 5days. The thermophilic reactors showed higher gas production than mesophilic reactor. Removal efficiencies of organic matter exceeded 80% on the basis of supernatants, except that at the reactor. Solid-liquid separation was essential in the performance of the ASBR, especially, at the lower HFT. The ASBRs were highly efficient in the retention of activated biomass within the reactor. thus compensating for increased equivalent organic loading rate through increased solids retention times followed by the increased solids, while maintaining shorter HRTs.