• Journal of Microbiology and Biotechnology
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• 제33권3호
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• pp.410-418
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• 2023

Bacilysin is a dipeptide antibiotic composed of L-alanine and L-anticapsin produced by certain strains of Bacillus subtilis. Bacilysin is gaining increasing attention in industrial agriculture and pharmaceutical industries due to its potent antagonistic effects on various bacterial, fungal, and algal pathogens. However, its use in industrial applications is hindered by its low production in the native producer. The biosynthesis of bacilysin is mainly based on the bacABCDEF operon. Examination of the sequence surrounding the upstream of the bac operon did not reveal a clear, strong ribosome binding site (RBS). Therefore, in this study, we aimed to investigate the impact of RBS as a potential route to improve bacilysin production. For this, the 5' untranslated region (5'UTR) of the bac operon was edited using the CRISPR/Cas9 approach by introducing a strong ribosome binding sequence carrying the canonical Shine-Dalgarno sequence (TAAGGAGG) with an 8 nt spacing from the AUG start codon. Strong RBS substitution resulted in a 2.87-fold increase in bacilysin production without affecting growth. Strong RBS substitution also improved the mRNA stability of the bac operon. All these data revealed that extensive RBS engineering is a promising key option for enhancing bacilysin production in its native producers.

• Asian-Australasian Journal of Animal Sciences
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• 제19권8호
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• pp.1159-1163
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• 2006

This experiment was performed to examine the influences of high ambient temperature on milk production, nutrient digestibility, energy and protein sufficiency ratio, and plasma metabolites concentration in lactating cows. In a $2{\times}2$ crossover design, four multiparous lactating Holstein cows were maintained in a chamber under treatment of constant moderate ($18^{\circ}C$) ambient temperature (MT) or high ($28^{\circ}C$) ambient temperatures (HT). The DMI and milk protein yield were significantly lower in HT (p<0.05). The milk yield, milk lactose yield, and milk SNF yield tended to be lower in HT (p<0.10). No statistical differences for 4% fat-corrected milk and milk fat yield were observed. Rectal temperatures were significantly higher in HT than MT (p<0.05). The apparent DM, OM, ether extract, CF, and ash digestibility did not differ between treatments. On the other hand, the apparent CP digestibility was increased significantly (p<0.05) and nitrogen free extract tended to increase (p<0.10) in HT. The sufficiency ratio of ME and DCP intake for each requirement tended to be lower in HT than in MT (p<0.10). Concentrations of total protein (TP), albumin, and urea nitrogen in plasma did not differ between treatments. Plasma 3-methylhistidine (3MH) concentration as a marker of myofibrillar protein degradation tended to be higher in HT (p<0.15). In conclusion, high ambient temperature was associated with a lower energy and protein sufficiency ratio, and decreased milk protein production, even though the body protein mobilization tended to be higher.

• Animal Bioscience
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• 제37권8호
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• pp.1474-1482
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• 2024

Objective: The experiment aimed to determine the standardized ileal digestibility (SID) of crude protein (CP) and amino acids (AA) in 10 brown rice samples fed to pigs, and to construct predictive models for SID of CP and AA based on the physical characteristics and chemical composition of brown rice. Methods: Twenty-two cannulated pigs (initial body weight: 42.0±1.2 kg) were assigned to a replicated 11×3 incomplete Latin square design, including an N-free diet and 10 brown rice diets. Each period included 5 d adaptation and 2 d ileal digesta collection. Chromic oxide was added at 0.3% to all the diets as an indigestible marker for calculating the ileal CP and AA digestibility. Results: The coefficients of variation of all detected indices for physical characteristics and chemical composition, except for bulk weight, dry matter (DM) and gross energy, in 10 brown rice samples were greater than 10%. The SID of CP, lysine (Lys), methionine, threonine (Thr), and tryptophan (Trp) in brown rice was 77.2% (62.6% to 85.5%), 87.5% (80.3% to 94.3%), 89.2% (78.9% to 98.9%), 55.4% (46.1% to 67.6%) and 92.5% (86.3% to 96.3%), respectively. The best prediction equations for the SID of CP, Lys, Thr, and Trp were as following, SID_CP = -664.181+8.484×DM (R² = 0.40), SID_Lys = 53.126+6.031×ether extract (EE)+0.893×thousand-kernel volume (R² = 0.66), SID_Thr = 39.916+7.843×EE (R² = 0.41), and SID_Trp = -361.588+4.891×DM+0.387×total starch (R² = 0.85). Conclusion: Overall, a great variation exists among 10 sources of brown rice, and the thousand-grain volume, DM, EE, and total starch can be used as the key predictors for SID of CP and AA.

• 대한의생명과학회지
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• 제26권3호
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• pp.136-148
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• 2020

A bispecific antibody (BsAb) is an artificial protein containing two kinds of specific antigen binding sites. BsAb can connect target cells to functional cells or molecules, and thus stimulate a directed immune response. Last several decades a wide variety of bsAb formats and production technologies have been developed. BsAbs are constructed either chemically or biologically, exploiting techniques like cell fusion and recombinant DNA technologies. There are over 100 different formats of bsAb so far developed, but they could be classified into the two main categories such as Fc-based (with a Fc region) bsAbs and fragment-based (without a Fc region) bsAbs. BsAb has a broad application prospect in tumor immunotherapy and drug delivery. Here, we present a brief introduction to the structure of antibody, pharmacological mechanisms of antibodies and the trend in the production technologies of therapeutic antibodies. In addition, we address a review on the current status of various bsAb format development and their production technologies together with global situation in the clinical studies of bsAb.

• Journal of Species Research
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• 제3권1호
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• pp.35-38
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• 2014

The article considers the influence of hunting (legitimate and uncontrolled) on the population of the Siberian roe deer, musk deer, elk, red and northern deer in Pribaikalje. The data on the forms and methods of hunting, the dynamics of the number of animals and the results of their production are given. For comparison, the acts of such a limiting factor as predators are specified. Reasons for the low number of wild ungulates are determined and main problems are formulated.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.99-99
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• 2022

This study was carried out to select the excellent silage maize varieties customized for paddy cultivation that is vulnerable to lodging and waterlogging in the central and region. Ten varieties (Kwangpyeongok, P3394, etc.) were sown with 3 replicates in Suwon, Gyeonggi-do. It was sown twice in April and June of each year in 2020-2021 and harvested during the yellow ripe stage suitable for silage. The number of days to flowering stage in lowland condition increased up to 11 days compared to that in upland condition. In April seedling, varieties that showed a relatively small decrease in biomass production and total digestible nutrients (TDN) under lowland condition compared to upland condition were Gangdaok and Kwangpyeongok in 2020, and Dacheongok, Gangdaok and Kwangpyeongok in 2021. Kwangpyeongok, Gangdaok, and Sinhwangok showed relatively higher biomass production and TDN than the other varieties under lowland compared to upland in both 2020 and 2021. Our results suggest that Kwangpyeongok and Gangdaok are suitable silage maize varieties for lowland cultivation in the central region of Korea.

• Journal of Microbiology and Biotechnology
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• 제23권10호
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• pp.1386-1394
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• 2013

In our previous study, the 25-mer antimicrobial peptide pleurocidin (Ple) had been thought to induce apoptosis in Candida albicans. This study demonstrated that reactive oxygen species (ROS) production was a major cause of Ple-induced apoptosis. Four truncated analogs were synthesized to understand the functional roles in the N- and C-terminal regions of Ple on the apoptosis. Ple, Ple (4-25), Ple (1-22), and Ple (1-19) produced ROS, including hydroxyl radicals, on the order of [Ple > Ple (1-22) > Ple (4-25) > Ple (1-19)], whereas Ple (7-25) did not induce any ROS production. The results suggested that the N-terminal deletion affected the ROS-inducing activities much more than that of the C-terminal deletion, and net hydrophobicity [Ple > Ple (1-22) > Ple (4-25) > Ple (1-19) > Ple (7-25)] was related to ROS generation rather than other primary factors like net charge. Hence, we focused on the N-terminal-truncated peptides, Ple (4-25) and Ple (7-25), and examined other apoptotic features, including mitochondrial membrane depolarization, caspase activation, phosphatidylserine externalization, and DNA and nuclear fragmentation. The results also confirmed the disappearance of apoptotic activity of Ple (7-25) by the truncation of the N-terminal region (1-6) and the specific activity patterns between Ple and analogs. In conclusion, the N-terminal region of Ple played an important role in apoptosis.

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.104-117
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• 2002

Objective : The aim of this study was to investigate the skin-whitening effect of Kakamseosiokyong-san Method : We investigated that the extracts of Kakamseosiokyong-san inhibit activity of tyrosinase, the enzyme which converts 3-(3,4-dihydroxyphenyl)alanine to dopachrom in the biosynthetic process of melanin. the UV absorbance of the extracts in the UV - A region and UV - B region was measured by UV scanning. the effect of extracts on cell viability and melanin production in cultured B16 mouse melanoma cells was measured, and cytoprotective effects of extracts on PC12 cells injured by hydrogen peroxide was measured by MTT assay Results: The extracts of Kakamseosiokyong-san inhibited activity of tyrosinase. The extracts not only showed inhibitory effects on melanin production in cultured B16 mouse melanoma cells, but also exhibited cytoprotective effects on PC12 cells injured by hydrogen peroxide, but did not showed an absorbance in the UV - A region and UV - B region. Conclusion: These results suggest that Kakamseosiokyong-san inhibit melanin biosynthesis which is involved in hyperpigmentation and could be used as a whitening agent for the skin.

• Journal of applied mathematics & informatics
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• 제19권1_2호
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• pp.165-177
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• 2005

In this paper, an adaptive trust region method based on the conic model for unconstrained optimization problems is proposed and analyzed. We establish the global and super linear convergence results of the method. Numerical tests are reported that confirm the efficiency of the new method.

• 만화애니메이션 연구
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• 통권45호
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• pp.147-175
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• 2016

본 논문은 2013년 이후 급변하는 한국의 만화산업에서 수도권에 집중된 창, 제작 환경의 지역 균형 발전을 위한 방안을 연구하기 위한 것이다. 만화는 여타 문화 콘텐츠 산업에 비해 제작비가 낮을 뿐만 아니라 제작기반이 최소한의 요건만 갖추어지면 가능하므로 지역 분권이 상대적으로 매우 용이한 분야이다. 현재 대부분의 만화 관련 기업, 진흥 기관 등은 서울과 부천 등에 분포하고 있다. 그러나 작가의 경우 전국적으로 분산되어 있으며 해외에서 작품 활동을 전개하는 작가도 유의미한 수치에 이른다. 대전, 부산, 순천, 경북 등에서는 만화 창 제작 센터를 구축하기 위해 2015년부터 본격적인 움직임을 보이고 있다. 본 논문에서는 지역별 만화 창 제작센터 구축 움직임을 해외 사례와 함께 살펴보고 각 지역별 특색과 균형, 조화를 어떻게 이룰 것인가를 점검해보고자 한다. 먼저 정부의 만화산업 육성 정책의 현황을 분석했다. 만화산업중장기발전계획을 축으로 한 정부의 만화진흥정책은 그간 한국콘텐츠진흥원과 부천의 한국만화영상진흥원을 축으로 전개되어 왔으나 웹툰 산업이 급성장하면서 각 지역에서도 진흥 기관의 필요성이 2015년 이후 제기되어 왔다. 2019년부터 시행하게 될 4차 만화산업 중장기발전계획 수립을 앞두고 본격적인 지역균형발전의 지원 틀이 갖춰져야 할 것으로 보인다. 해외의 경우는 미국 샌디에고(코미콘), 프랑스의 앙굴렘(국립이미지센터), 일본의 교토(만화박물관), 사카이미나토(미즈키시게루로드) 등 일찍부터 지역을 중심으로 만화진흥 기관, 행사 등이 발달해 왔던 점은 시사하는 바가 크다. 결론에서는 지역마다 만화창 제작센터가 구축된다고 하더라도 지역의 성격과 특징에 맞게 설립되는 것의 중요성과 필요성을 구체적인 방안을 들어 제안하고자 했다. 이를 바탕으로 문화콘텐츠 산업에서 거의 유일하게 지역 창 제작 시스템이 정착될 수 있는 만화, 웹툰 산업의 비전을 제시하고자 한다.