• Journal of Animal Science and Technology
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• 제46권5호
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• pp.729-734
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• 2004

본 연구는 한우의 mtDNA D-loop 영역 염기 변이가 산유량에 미치는 효과를 검증하기 위하여 수행하였다. 젖소의 mtDNA D-loop 영역에서 단일염기의 치환이 20 개의 지역에서 확인되었다. 그중 8, 169, 16042, 16051, 16057, 16093, 16119, 16122, 16209, 16255, 16302 bp 지역에서 높은 빈도의 염기치환이 검출되었다. 16119bp 지역에서는 T가 C로 치환된 빈도가 0 .138로 검출되었으며, 이 위치에서의 염기치환에 의한 산유량 효과는 -1.61 (p<0.1)로 나타났다. 16185bp 지역에서의 염기치환에 의한 산유량 효과는 3.557(p<0.05)로 나타났으며 G가 A로 치환된 빈도는 0.063로 나타났다. 본 연구에서 검출한 한우 다유계통 집단의 mtDNA내 D- loop 영역의 염기서열 변이 빈도와 유량과의 연관성분석 결과 등은 한우 집단의 유전적 변이성 추정과 좀 더 다양한 경제형질과의 관련성 분석으로 다양한 유전적 지표인자 발굴에 도움 될 것이며 이를 통해 분자유전학적 기법을 이용한 한우의 육종 전략을 확립하논데 기초 자료가 되는 것은 물론 우려나라 고유 품종인 한우의 유전자원 보존과 분자육종학적인 연구에 기초 자료로서 유용하게 활용 할 수 있을 것으로 기대된다.

• 지역과문화
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• 제7권2호
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• pp.1-29
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• 2020

지역문화진흥법의 시행과 함께 문화매개 영역에 대한 관심이 증가하는 가운데, 본 연구자는 지역에서 독립적으로 활동해 온 문화매개자의 역할을 기술할 수 있는 이론적 논의와 사례 연구를 모색하였다. 이에 이 연구는 지역의 문화매개 활동을 지역과 문화를 둘러싼 능동적인 의미 생산 활동으로 의미화하고, 기존의 가치와 인식에 대해 대안적 의미를 생산하고 지역의 문화 공론장을 역동적으로 구성하는 데 기여하는 문화매개자 역할을 조명하는 데 그 목적을 두었다. 피에르 부르디외의 개념을 경유하여 지역을 둘러싼 의미 생산에 기여하는 문화매개자의 역할을 이론적으로 검토한 후, 지역과 문화의 매개가 기존의 중앙 집중화된 문화생산 구조에 대해 대안적 의미를 생산하고, 도시재생의 국면에서 지역의 구체성을 드러내는 로컬 기획이 문화 공론장을 역동적으로 구성하는 역할을 수행할 수 있다는 점을 논하였다. 구체적인 논의를 위해 인천에서 활동해 온 로컬 기획자(스페이스빔, 임시공간, 인천스펙타클)의 사례를 연구하였다. 연구 결과, 각각의 사례는 1) 주민들과 이슈를 공유하고 지역의 대안을 제시함으로써 삶의 환경을 변화시키는 역할 2) 지역의 전문예술 영역을 공적인 장으로 재구성하는 역할 3) 독립출판물을 통해 지역의 일상문화를 드러내고 네트워크를 형성하는 역할을 보여주었다. 이들은 각자의 영역과 관심 속에서 지역의 다양한 면모를 드러내는 과정을 통해 인천에서 문화를 둘러싼 공적 영역의 구성에 관여하고 있는 것으로 확인되었다. 이번 연구는 지역문화의 자율성과 다양성의 확보라는 과제를 둔 상황에서, 문화매개자의 역할을 지역에서 대안적 의미를 생산하는 활동주체로 의미화하고 각각의 매개 실천들의 특이성을 분석하고자 했다는 점에서 의미를 가진다.

• Applied Biological Chemistry
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• 제40권1호
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• pp.58-64
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• 1997

카이이케호에서 농밀하게 분포하는 Chromatium sp.의 수소생산을 몇몇 환경조건하에서 측정하였다. 최대수소생산 ($0.01\;{\mu}mol/hr/(mg\;dry\;cell\;weight)$)은 낮은 조도 (1000 lux), 20 mg HS-S/l의 황화수소 농도와 $30^{\circ}C$의 조건하에서 얻을 수 있었다. 수소생산은 질소가스 또는 암모늄에 의해 상당히 저해를 받았다. Chromatium sp.의 수소생산속도는 타 광합성세균에 비해 낮았다. 본 균은 광독립영양적으로 낮은 조도 또는 낮은 $H_2S$ 농도하에서도 쉽게 수소를 생산하는 점으로부터 카이이케흐의 가장 유력한 수소생산자라고 고려되었다. 이러한 Chromatium sp.의 광독립영양적 수소생산특성으로부터, 본 광합성세균은 온대역에서의 생물적 수소생산시스템에 대한 경제적 이고 실용적인 생물종이 될 수 있으리라 제안되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제18권3호
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• pp.308-313
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• 2005

Logistic Regression Analysis was used to compute the odds ratio (OR) and probability of conception of Holstein dairy cows of AL-MARAIE company. Data consisted of 103,778 reproductive records collected from three farms in the central region in the Kingdom of Saudi Arabia. Records were classified according to lactation number, season of calving and milk level. At first lactation, OR of first service was 0.63 of other services and probability of pregnancy from first services was 0.39. Odds ratio increased to 1.72 at fourth lactation or probability of conception reached 0.63. The probability of conception increased from 0.39 for cows inseminated at first services to 0.75 at fifth service insemination. Odds ratio of cows calving in winter were higher than those calving in summer. Odds ratio of conception of low producing cows is about twice as likely to occur from first service as from other services. However, OR of conception of high producing cows was higher than one (probability=0.56) from first service and increased to 1.63 (probability=0.65) from third service. Odds ratio was in favor of the right uterus horn where probability of conception from first service was slightly greater than 50% in first and second lactations and less than 50% in favor of left horn in later lactations.

• Asian-Australasian Journal of Animal Sciences
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• 제13권3호
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• pp.401-412
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• 2000

Like other livestock, monogastric animals are essential components of the farming systems in the tropical countries. Pigs, chicken and ducks are by far the most important animals in the culture of the peoples of developing countries in the tropics. Traditionally these animals are raised in small farms and they are also the bulk producers of meat, eggs etc. in the tropics. In many countries the farmers of these small farms are unable to meet the requirement set by financial institution and other loan giving agencies for agricultural loan. Thus, the small farmers can get neither the opportunity to generate sufficient income to support the family nor to extend the livestock activities. The production systems are characterized by small number of animals with no or minimal inputs, low outputs and periodic destruction of animals by disease. Typically the litter size or flocks are small in number with each household containing 5-6 pigs and 7-10 poultry. Animals are owned by individual households and mostly maintained under a scavenging systems with little or no inputs for housing, feeding or health care. Because of the nature of this production system, productivity of these animals is rather low. The low level of inputs is due to a lack of capital and a low risk oriented outlook. The feed resource base for monogastric is scavenging and consists of household waste, roots and tuber, grain by-products and anything edible found in the immediate environment. Usually farmers select breeding gilts from their own female piglets or to a lesser extent, buy them from neighbors for natural mating. As regards poultry attempts have been made to increase egg and meat production by improving local poultry birds by upgrading and crossbreeding with exotic germ plasma in the tropics. Animal disease present a major constraint to animal production in the tropical region and the extent of the losses due to disease is very high.

• International journal of advanced smart convergence
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• 제9권1호
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• pp.82-89
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• 2020

This study is a design study related to the application of 3D printer technology for garment printing. Knitting, which used to be a home industry in the early days, originally comes from hand-knitting. This evolved with various personal devices as a small job for the self-sufficiency of early European housewives. In addition, since the Industrial Revolution, mechanical production entered the mass production mass supply system, and various apparel products were provided to consumers in accordance with mass standardized dimensions. This is similar to the development process in Korea. In addition, it has formed a considerable market with the situation that it can produce and supply apparel products at low labor costs of first-generation Namdaemun and Dongdaemun merchants. As the production shifted to the Southeast Asian region due to the increase in labor costs in the domestically developed social situation, the production of garment products in Korea is now almost 5%. As a result, apparel-related production facilities and related companies are constantly moving to other countries to move production facilities sensitively due to rising labor costs. Recently, smart factory automation has been planned to explore new possibilities. In addition, in recent years, with the evolved appearance of consumers, the appropriate supply of the right amount of production has appeared, and the 3D printer applied to personal garment output has attracted considerable interest in the customized market. Therefore, in order to become a new hope and a small addition to various clothing workers, this study conducted related research on the following 3D printers for clothing output and attempted to proceed with a new design.

• Mycobiology
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• 제45권4호
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• pp.362-369
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• 2017

We assessed the regulation of cryparin, a class II hydrophobin, using three representative mitogen-activated protein kinase (MAPK) pathways in Cryphonectria parasitica. Mutation of the CpSlt2 gene, an ortholog of yeast SLT2 in the cell wall integrity (CWI) pathway, resulted in a dramatic decrease in cryparin production. Similarly, a mutant of the CpBck1 gene, a MAP kinase kinase kinase gene in the CWI pathway, showed decreased cryparin production. Additionally, mutation of the cpmk1 gene, an ortholog of yeast HOG1, showed decreased cryparin production. However, mutation of the cpmk2 gene, an ortholog of yeast Kss1/Fus3, showed increased cryparin production. The easy-wet phenotype and accumulation of the cryparin transcript in corresponding mutants were consistent with the cryparin production results. In silico analysis of the promoter region of the cryparin gene revealed the presence of binding motifs related to downstream transcription factors of CWI, HOG1, and pheromone responsive pathways including MADS-box- and Ste12-binding domains. Real-time reverse transcriptase PCR analyses indicated that both CpRlm1, an ortholog of yeast RLM1 in the CWI pathway, and cpst12, an ortholog of yeast STE12 in the mating pathway, showed significantly reduced transcription levels in the mutant strains showing lower cryparin production in C. prasitica. However, the transcription of CpMcm1, an ortholog of yeast MCM1, did not correlate with that of the mutant strains showing downregulation of cryparin. These results indicate that three representative MAPK pathways played a role in regulating cryparin production. However, regulation varied depending on the MAPK pathways: the CWI and HOG1 pathways were stimulatory, whereas the pheromone-responsive MAPK was repressive.

• Asian-Australasian Journal of Animal Sciences
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• 제32권1호
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• pp.82-91
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• 2019

Objective: This experiment was conducted to compare the structure and composition of ruminal microorganisms in goats with high and low neutral detergent fibre (NDF) digestibility. Methods: Nineteen crossbred goats were used as experimental animals and fed the same total mixed rations during the 30-day pre-treatment and 6-day digestion trialperiods. All faeces were collected during the digestion period for measuring the NDF digestibility. Then, high and the low NDF digestibility individuals were chosen for the high NDF digestibility group (HFD) and low NDF digestibility group (LFD), respectively. Rumen contents were collected for total microbial DNA extraction. The V4 region of the bacterial 16S rRNA gene was amplified using universal primers of bacteria and sequenced using high-throughput sequencer. The sequences were mainly analysed by QIIME 1.8.0. Results: A total of 18,694 operational taxonomic units were obtained, within 81.98% belonged to bacteria, 6.64% belonged to archaea and 11.38% was unassigned microorganisms. Bacteroidetes, Firmicutes, and Proteobacteria were the predominant microbial phyla in both groups. At the genus level, the relative abundance of fifteen microorganisms were significantly higher (p<0.05) and six microorganisms were extremely significantly higher (p<0.01) in LFD than HFD. Overall, 176 core shared genera were identified in the two groups. The relative abundance of 2 phyla, 5 classes, 10 orders, 13 families and 15 genera had a negative correlation with NDF digestibility, but only the relative abundance of Pyramidobacter had a positive correlation with NDF digestibility. Conclusion: There were substantial differences in NDF digestibility among the individual goats, and the NDF digestibility had significant correlation with the relative abundance of some ruminal microorganisms.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8_spc호
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• pp.1266-1274
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• 2019

This paper presents a review of dairy goat production in sub-Saharan Africa (SSA) from 2010- 2017, its current state, constraints and prospects for research and development. Since the introduction of dairy goats in SSA in pre-colonial times, their populations have continued to increase due to declining land size as a result of land fragmentation and increasing demand for goat milk. The current goat population in SSA is 372,716,040 head of which only 15.98% used for milk production. Populations in the Eastern and Western regions of SSA have shown an increasing trend from 2010 to 2017. The Southern Africa goat population is on the decline at an annual rate of about 1.77% whereas Central Africa has had a constant goat population within the same period. Eastern Africa reported the highest increase in the population of goats used for milk production. Milk production was highest in Eastern Africa and lowest in Southern Africa. However, dairy goat productivity remained constant in the Eastern region throughout the review period. Dairy goats are mainly raised under smallholder mixed crop-livestock systems. To enhance the development of the dairy goat, concerted efforts should be made to alleviate the constraints that stifle its growth. These constraints can be categorized into nutrition and feeding, breeding and reproduction, diseases, parasites, climate change, and underdeveloped dairy goat products market. Effective management of dairy goats requires a holistic approach and there is the need to expand the markets by further sensitization on the nutritional and medicinal advantages of dairy goat products. In order to achieve rapid development in the dairy goat sub sector, research and development initiatives should be directed towards alleviating the hurdles in nutrition and feeding, breeding, animal health and resilience as well as dairy goat markets.

• Asian-Australasian Journal of Animal Sciences
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• 제18권11호
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• pp.1548-1551
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• 2005

The leptin, an anti-obesity protein, is a hormone protein expressed and secreted mainly from adipocyte tissue, and involved in regulation of body weight, food intake and energy metabolism. In an effort to discover polymorphism(s) in genes whose variant(s) might be implicated in phenotypic traits of growth, we have sequenced exons and their boundaries of leptin gene including 1,000 bp upstream of promoter region with twenty-four unrelated Korean cattle. Fifty-seven sequence variants were identified: fourteen in 5' flanking region, twenty-seven in introns, eight in exons, and eight in 3' flanking region. By pair-wise linkage analysis among polymorphisms, ten sets of SNPs were in absolute linkage disequilibrium (LD) (|D'| = 1 and $r^2$ = 1). Among variants identified, thirty-six SNPs were newly identified, and twenty-one SNPs, which were reported in other breeds, were also confirmed in Korean cattle. The allele frequencies of variants were quite different among breeds. The information from SNPs of bovine leptin gene could be useful for further genetic studies of this gene.