• Molecules and Cells
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• 제40권2호
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• pp.109-116
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• 2017

Soybean agglutinin (SBA) is an anti-nutritional factor of soybean, affecting cell proliferation and inducing cytotoxicity. Integrins are transmembrane receptors, mediating a variety of cell biological processes. This research aims to study the effects of SBA on cell proliferation and cell cycle progression of the intestinal epithelial cell line from piglets (IPEC-J2), to identify the integrin subunits especially expressed in IPEC-J2s, and to analyze the functions of these integrins on IPEC-J2 cell cycle progression and SBA-induced IPEC-J2 cell cycle alteration. The results showed that SBA lowered cell proliferation rate as the cell cycle progression from G0/G1 to S phase (P < 0.05) was inhibited. Moreover, SBA lowered mRNA expression of cell cycle-related gene CDK4, Cyclin E and Cyclin D1 (P < 0.05). We successfully identified integrins ${\alpha}2$, ${\alpha}3$, ${\alpha}6$, ${\beta}1$, and ${\beta}4$ in IPEC-J2s. These five subunits were crucial to maintain normal cell proliferation and cell cycle progression in IPEC-J2s. Restrain of either these five subunits by their inhibitors, lowered cell proliferation rate, and arrested the cells at G0/G1 phase of cell cycle (P < 0.05). Further analysis indicated that integrin ${\alpha}2$, ${\alpha}6$, and ${\beta}1$ were involved in the blocking of G0/G1 phase induced by SBA. In conclusion, these results suggested that SBA lowered the IPEC-J2 cell proliferation rate through the perturbation of cell cycle progression. Furthermore, integrins were important for IPEC-J2 cell cycle progression, and they were involved in the process of SBA-induced cell cycle progression alteration, which provide a basis for further revealing SBA anti-proliferation and anti-nutritional mechanism.

• 한국경영과학회지
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• 제31권2호
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• pp.57-67
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• 2006

One of challenging issues of market oriented manufacturing organization is product proliferation. However, increasing product variety not only incurs additional direct and indirect production cost, but also aggravates production system performance such as inventory level, customer lead time and fill rate. Recently, as one of the most beneficial strategic choices to menage the risks associateed with product variety and uncertain sales, special attention is focused on the product/process redesign to delayed product differentiation. In this paper, we develop an analytical model to demonstrate the existence of an optimal level of product differentiation, which can be used to provide theoretical basis in establishing market oriented SCM strategy.

• 마케팅과학연구
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• 제19권4호
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• pp.24-31
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• 2009

公司生产不同功能, 设计, 颜色的产品. 产品扩散的出现有三个不同的原因. 当存在规模经济, 当这种产品和别的产品一起生产时, 单位成本比单独生产要低. 二, 消费者是异构的, 即它们具有不同的品味, 喜好, 或价格弹性. 一家公司可赚取细分为具有类似特点的不同群体的消费者更多的利润. 例如, 产品扩散通过更准确地满足不同消费者的需要来帮助公司增加利润. 产品扩散的第三个原因是基于战略. 生产一定数量的产品, 不仅可以阻止通过提供给一些少数市场的产品进入, 也使得公司可以有效地应付低价格进入. 通过生产各种产品, 公司可以减少利基, 使潜在进入者有较少进入的诱因. 此外, 企业可以生产新产品来应对进入, 我们称之为战斗品牌. 也就是说, 当一个进入者试图以低廉的价格吸引消费者, 已存在者介绍新的低质量的产品, 同时保持现有产品的价格. 产品扩散的缺点是同型装配. 一些买了高价位的产品的消费者会转向低价位的产品. 此外, 当新产品与对手现有的产品的异化程度不高时, 由于激烈的竞争, 产品扩散会降低利润. 许多研究已经在经济分析和市场营销等领域的产品线竞争的影响. 它们展示了一个垄断者可以通过调整质量来解决市场中的消费者对质量的偏好不同的同型装配的问题. 他们发现, 喜欢高品质的产品的消费者将获得他或她最喜欢的质量, 但没有这方面的偏好的消费者将获得比他或她所喜爱的质量低的产品. 本研究分析了产品的竞争在一个双头垄断市场, 两种不同类型的消费者对质量偏好的影响. 我假设这两家公司将在这个意义上的不对称, 一个运营商可以同时生产高, 低质量的产品, 而一个进入者只能产生低质量的产品. 产品扩散的影响是可以通过比较市场结果来解释, 当已存在的运营商生产两种产品和只生产一种产品时. 在这个案例中, 当已存在的运营商只生产高品质的产品, 由于激烈的竞争, 在喜欢低质量产品的消费群中低质量的产品价格趋于下降. 但由于缺乏竞争, 在喜欢高质量产品的消费群中价格会上涨. 据了解, 当企业在进行价格竞争时, 理想状况是当公司的对 手提高价格时, 此公司也提高价格, 这被称为战略补充. 由于价格是战略性的补充, 我们有两种相反的效果. 事实证明, 一个高品质的产品价格上升, 因为竞争力减弱的积极作用超过了战略互补的负面影响. 这意味着, 已存在的运营商推出了低质量的产品时还需要增加高品质产品的价格. 然而, 在进入者的低质量产品的价格变化是模糊的. 二, 此案例中, 已存在的运营商只生产低品质的产品, 在偏好低质量的消费群中价格往往增加. 但在偏好高质量的消费群中价格往往下降. 低质量产品的价格下降是因为负面影响大于正面影响. 而且, 当已存在的运营商生产两种产品时, 其低质量产品的价格往往较高, 尽管两家的低品质的产品质量一样. 此原因由于对高品质产品价格的负面影响, 运营商没有较大的动机去降低低品质产品的价格. 事实上, 竞争的产品线对利润的影响不仅取决于价格变化, 还取决于销售和同型装配. 如果在边际成本同产品质量的差异相比是适中的话, 产品扩散的积极影响大于负面影响, 从而增加利润. 此外, 如果成本差异是非常大(小), 运营商最好只生产一种低 (高)质量的产品. 而且, 本研究还分析了当公司通过关注战斗品牌来决定产品特征时, 竞争产品线的影响. 最近, 大韩航空和韩亚航空公司建立了廉价航空线路, 分别是Jin线路和釜山线. 来应对Hansung航空和济州航空. 另外, 很多网上书店也进入市场, 例如处于领先地位的实体书店Kyobo已经有了自己的网上书店. 通过战斗品牌, 在它的低品质产品跟新成员比起来有差别时, 一个具有高品质产品的运营商通过生产更多的低质量产品可以增加利润.

• Asian-Australasian Journal of Animal Sciences
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• 제19권11호
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• pp.1665-1670
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• 2006

The objective of this research was to provide the characterization and method for producing anti-E. coli O157:H7 antibodies in egg-laying hens and to determine if the antibody can restrain the proliferation of E. coli O157:H7 in-vitro. Selected antigenic fractions (whole cell, outer membrane protein and lipopolysaccharide (LPS)) from E. coli O157:H7 were injected to hens in order to produce anti-E. coli O157:H7 antibodies. The immune response and the egg yolk antibodies of laying hens against the whole cell, outer membrane protein and LPS antigens were monitored by ELISA. The level of antibodies against whole cell antigen monitored through ELISA sharply increased after the initial immunization, and it was found to be maximum on day 49 however, the level was maintained up to day 70. Antibodies (5 mg/ml) directed against the whole cell inhibited E. coli proliferation 10-13 times more than outer membrane protein or LPS. The antibody response against the whole cell antigens appeared to have higher activity in restraining the proliferation of E. coli O157:H7 than antibody against outer membrane protein or LPS. Results reflected that increasing the IgY's in the egg yolk could prevent greater economic losses due to human and animal health from pathogenic bacteria i.e. E. coli O157:H7.

• Nuclear Engineering and Technology
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• 제40권7호
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• pp.581-592
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• 2008

In this study, a conceptual pyroprocess flowsheet has been devised by combining several dry-type unit processes; its applicability as an alternative fuel cycle technology was analyzed. A key point in the evaluation of its applicability to the fuel cycle was the recovery yield of fissile materials from spent fuels as well as the proliferation resistance of the process. The recovery yields of uranium and transuranic elements (TRU) were obtained from a material balance for every unit process composing the whole pyroprocess. The material balances for several elemental groups of interest such as uranium, TRU, rare earth, gaseous fission products, and heat generating elements were calculated on the basis of the knowledge base that is available from domestic and foreign experimental results or technical information presented in open literature. The calculated result of the material balance revealed that uranium and TRU could be recovered at 98.0% and 97.0%, respectively, from a typical PWR spent fuel. Furthermore, the anticipated TRU product was found to emit a non-negligible level of $\gamma$-ray and a significantly higher level of neutrons compared to that of a typical plutonium product obtained from the PUREX process. The results indicate that the product from this conceptual pyroprocessing should be handled in a shielded cell and that this will contribute favorably to retaining proliferation resistance.

• 생약학회지
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• 제40권1호
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• pp.77-81
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• 2009

Abies nephrolepis(Pinaceae) extracts were tested for determined immune system regulating activity based on antiinflammatory activity, antioxidant activity and anti-proliferative effect on HeLa cell line. The A. nephrolepis extracts increased dose-dependently anti-proliferation of HeLa cell line. The DM fraction of the extracts having anti-proliferatative effects of HeLa cell line was fractionalized four subfractions($D1{\sim}D4$). Inflammation-induced NO production was inhibited by D2 and D4 in LPS-activated RAW264.7 macrophages. And also, this fractions showed antioxidant activity examined by DPPH radical scavenging effects. These results suggest that the potential use of DM fraction of A. nephrolepis in chemoprevention and regulation overproduction of NO on pathogenic conditions. The mechanism of the inflammatory effects, however, must be evaluated through various parameters in the induction cascade of NO production.

• 생약학회지
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• 제37권3호
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• pp.212-216
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• 2006

The effect of aqueous extracts of medicinal plants $AMP-365^{TM}$ was tested for immune system regulating activity based on anti-inflammatory activity, anti-oxidant, macrophage proliferation and T-lymphocyte proliferation activity. $AMP-365^{TM}$ dose-dependently increased proliferation of RAW264.7 macrophage cells and its nitric oxide production as well as DPPH radical scavenging activity. On the other hand, T-lymphocyte proliferation activity was decreased on dose-dependent manner. Passive cutaneous anaphylaxis reaction was alleviated by 49% by administering 250 mg/kg of $AMP-365^{TM}$. The results suggest that $AMP-365^{TM}$ can be beneficial in the treatment of immediate allergic reactions as an adjuvant supplement material.

• Industrial Engineering and Management Systems
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• 제8권1호
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• pp.47-53
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• 2009

We define product line (or mix) selection problem as selecting a subset of potential product variants that can simultaneously minimize product proliferation and maintain market coverage. Selecting the most efficient product mix is a complex problem, which requires analyses of multiple criteria. This paper proposes a method based on Data Envelopment Analysis (DEA) for product line selection. Data Envelopment Analysis (DEA) is a linear programming based technique commonly used for measuring the relative performance of a group of decision making units with multiple inputs and outputs. Although DEA has been proved to be an effective evaluation tool in many fields, it has not been applied to solve the product line selection problem. In this study, we construct a five-step method that systematically adopts DEA to solve a product line selection problem. We then apply the proposed method to an existing line of staplers to provide quantitative evidence for managers to generate desirable decisions to maximize the company profits while also fulfilling market demands.

• 한국경영과학회지
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• 제29권1호
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• pp.101-112
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• 2004

Customer satisfaction level is usually measured in terms of price, qualify, customization, after-sale-service, product variety etc. Each firm sets up a distinctive production/marketing strategy to gain competitive advantage by Prioritizing the customer satisfaction measures. The market differentiation strategy directly results in supply chain performance such as lead time, inventory, customer fill rate and market share. Product proliferation desirable in customization sense often conflicts the economies of scale effect in production side. This paper focuses the relationship between product variety and market share. Specifically, we investigate how introduction of new product affects the current market share, i.e., formation of customer preference and provide some insight into the optimal range of product variety.

• Journal of Nutrition and Health
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• 제52권6호
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• pp.515-528
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• 2019

본 연구는 괭생이 모자반 추출물의 추출방법에 따른 면역증강활성에 관하여 비교 관찰하였고, 추출물간의 혼합비율을 다르게 혼합하여 제조한 괭생이 모자반 추출물의 최적 면역활성을 갖는 혼합비를 평가하였다. 비장세포에 괭생이 모자반 열수 (HWE), 주정 (EE), 초임계 (SFE) 추출물을 각각 처리하였을 때 HWE 및 SFE 처리구에서 비장세포 증식능 및 사이토카인 (IL-2, IFN-γ) 분비능이 높게 나타나는 것으로 관찰되었고, 각각의 추출물을 50, 100 mg/kg BW의 농도로 2주간 마우스에 투여하고 비장을 적출하여 비장세포 활성능에 관하여 관찰한 결과, HWE 및 SFE 투여구에서 비장세포 증식능 및 사이토카인 분비능이 높게 나타나는 것으로 관찰되었다. 동물실험에서 높은 활성을 보인 HWE와 SFE의 추출물의 혼합 최적비를 평가하기 위하여 HWE에 SFE를 5, 10, 15 및 20%로 혼합하여 대식세포에 처리하여 NO 및 사이토카인 분비능에 관하여 관찰한 결과, SFE를 5%, 10% 혼합한 추출물 처리구에서 활성이 높게 나타나는 것으로 관찰되었으며 혼합추출물을 100 mg/kg BW으로 14일간 투여한 마우스의 비장세포 활성에 관하여 관찰한 결과, SFE를 5%의 비율로 혼합한 추출물 처리구 비장세포 활성이 높게 나타나는 것으로 관찰되어 HWE와 SFE의 최적 혼합 비율을 HWE에 SFE를 5% 첨가하는 것으로 결정하였다. 결정된 최적혼합비로 100 mg/kg BW의 농도로 14일간 투여한 마우스의 비장세포에서 NK 세포를 분리하여 NK 세포의 활성능을 평가한 결과, 대조구 대비 높게 증가하는 것으로 나타났다. 따라서 괭생이 모자반 추출물을 최적비율로 혼합하여 제조한 혼합추출물은 비장세포, 대식세포 및 NK 세포에 대한 높은 활성능을 나타내어 면역증진에 기여하는 것으로 판단된다.