• Journal of the Korean Magnetic Resonance Society
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• v.19 no.2
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• pp.74-82
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• 2015

Ryanodine receptor (RyR) is one of the two major $Ca^{2+}$ channels in membranes of intracellular $Ca^{2+}$ stores and is found in sarcoplasmic reticulum (SR), endoplasmic reticulum (ER). RyR1 is also the major calmodulin-binding protein of sarcoplasmic reticulum membranes. Residues 4064-4210 in the RyR1 polypeptide chain has similar primary sequence with calmodulin (CaM) and was designated as CaM-like domain (CaMLD). When expressed as a recombinant peptide, CaMLD showed several CaM-like properties in previous studies. Still, previous studies of CaMLD were focused on protein-protein interactions rather than its own properties. Here, we studied the expression of CaMLD and its sub-domains corresponding to each lobe of CaM in Escherichia coli. CaMLD could be obtained only as inclusion body, and it was refolded using urea solubilization followed by dialysis. Using spectroscopic approaches, such as NMR, circular dichroism, and gel filtration experiment, we found that the refolded CaMLD exists as nonspecific aggregate, even though it has alpha helical secondary structure. In comparison, the first half of CaMLD (R4061-4141) could be obtained as natively soluble protein with thioredoxin fusion. After the removal of the fusion tag, it exhibited folded and helical properties as shown by NMR and circular dichroism experiments. Its oligomeric status was different from CaMLD, existing as dimeric form in solution. However, the second half of the protein could not be obtained as soluble protein regardless of fusion tag. Based on these results, we believe that CaMLD, although similar to CaM in sequence, has quite different physicochemical properties and that the second half of the protein renders it the aggregative properties.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.19 no.1
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• pp.48-55
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• 2018

A framework has recently been developed to evaluate the user experience (UX) design for a virtual reality (VR) indoor bike. However, no comparative evaluation has been performed for an actual product using this framework. This paper investigates the effectiveness of the framework and identifies some problems when applying it. To validate the framework, we applied the proposed framework to a Korean VR indoor bike (KoVIB) prototype and VirZOOM, a commercial VR bike. The evaluation criteria consist of usability, emotional aspects, user values, and sense of reality. These four criteria were interpreted to evaluate prototypes and include 13, 6, 8, and 16 sub-elements, respectively. The results revealed the strengths and weakness between the Korean prototype and the commercial product, which are valuable for promotion and improvement. The framework helps to identify some problems when applied to real domains. As a result, we recommend a revised framework to compare prototypes with existing VR bikes.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.660-664
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• 2000

To clone genes related UK-58,852 production, genomic DNA of strain Actinodura roseorufa was used for the construction of genomic library using pOJ446 cosmid vector. The genomic library was screened rising dehydratase PCR product and eryA gene as a DNA hybridization probe. pHD54 was isolated, which contained an approximately 35kb of inserted DNA. BamHI, SmaI and sonicater fragments hybridized to eryA probe. All of pHD54 BgmHI, SmaI and sonicater fragments were subcloned into pGEM7 and some fragments which hybridized to eryA probe were sequenced. The nucleotide sequence was analysed using BLAST program. The sequence identities were observed in KS,AT, KR, ER and PKS loading domains. Also oxidoreductase showed similarity to rifamycin module10, and dTDP-D-glucose 4,6 dehydratase and TDP-D-glucose synthase involved in biosynthesis of sugar showed similarity to Streptomyces argillaceus.

• Mycobiology
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• v.42 no.1
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• pp.34-40
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• 2014

Usnea longissima has a long history of use as a traditional medicine. Several bioactive compounds, primarily belonging to the polyketide family, have been isolated from U. longissima. However, the genes for the biosynthesis of these compounds are yet to be identified. In the present study, three different types of non-reducing polyketide synthases (UlPKS2, UlPKS4, and UlPKS6) were identified from a cultured lichen-forming fungus of U. longissima. Phylogenetic analysis of product template domains showed that UlPKS2 and UlPKS4 belong to group IV, which includes the non-reducing polyketide synthases with an methyltransferase (MeT) domain that are involved in methylorcinol-based compound synthesis; UlPKS6 was found to belong to group I, which includes the non-reducing polyketide synthases that synthesize single aromatic ring polyketides, such as orsellinic acid. Reverse transcriptase-PCR analysis demonstrated that UlPKS2 and UlPKS4 were upregulated by sucrose; UlPKS6 was downregulated by asparagine, glycine, and alanine.

• Journal of the Korean Society of Marine Environment & Safety
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• v.23 no.3
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• pp.250-257
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• 2017

This paper investigates minimum safe distances relative to a ship's four cardinal sides, as perceived by Filipino navigators when encountering dangerous elements and in adverse weather conditions when maneuvering in and around harbors. It uses a descriptive research method in the form of a questionnaire survey for experienced Filipino navigators of various ranks. During the course of research, 71 responses were colleted and the resulting data is presented in graphical and tabulated forms. Statistical methods including Pearson-product moment correlations, Cronbach's Alpha and ANOVA were used to identify internal associations, consistencies and significances, respectively. It has been proven that there are no significant differences in minimum safe distances relative to a ship's four cardinal sides, whether maneuvering while approaching a port or within an inner harbor. This study has been deemed significant for training future navigators, managing traffic in fairways, and designing harbors and maneuvering areas in the approaches to ports, among other applications. This work can also be used as a preliminary study for comparison with the well known safe domains presently in use.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.35 no.7
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• pp.657-664
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• 2011

This study proposes an inverse method for estimating the boundary temperature in a gas-filled, onedimensional parallel domain enclosed by parallel plates. The distance between the plates is considered submicron to one mm. In the current method, it is assumed that the conditions of both heat flux and temperature are simultaneously applicable to one boundary, while no conditions are applicable to the other boundary The temperature on one of the boundaries should be inversely determined from the known temperature and heat flux on the other boundary. This study proposes a procedure for estimating the unknown boundary temperature through Monte Carlo simulation. Both the forward and inverse problems employ the Monte Carlo approach. The forward (direct) problem is solved by using the direct simulation Monte Carlo while the inverse solution is obtained by the simulated annealing.

• Journal of Applied Biological Chemistry
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• v.48 no.3
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• pp.113-119
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• 2005

O-methylation mediated by O-methyltransferases (OMTs) is a common modification in natural product biosynthesis and contributes to diversity of secondary metabolites. OMTs use phenylpropanoids, flavonoids, other phenolics and alkaloids as substrates, and share common domains for S-adenosyl-L-methionine (AdoMet) and substrate binding. We searched Arabiposis genome and found 17 OMTs genes (AtOMTs). AdoMet- and substrate-binding sites were predicted. AdoMet binding domain of AtOMTs is highly conserved, while substrate-binding domain is diverse, indicating use of different substrates. In addition, expressions of six AtOMT genes in response to UV and in different tissues were investigated using real-time quantitative reverse transcriptase-polymerase chain reaction. All the AtOMTs investigated were expressed under normal growth condition and most, except AtOMT10, were induced after UV illumination. AtOMT1 and AtOMT8 were expressed in all the tissues, whereas AtOMT10 showed flower-specific expression. Analysis of these AtOMT gene expressions could provide some clues on AtOMT involvement in the cellular processes.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1993.10a
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• pp.1222-1229
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• 1993

An advanced design environment , which is based on adaptive and knowledge -based finite elements (INTELMESH), has been developed. Unlike other approaches, INTEMMESH incorporates the information about the object geometry as well as the boundary and loading conditions to generate an ${\alpha}$-priori finite element mesh which is more refined around the critical regions of the problem domain. INTEMMESH is designed for planar domains and axisymmetric 3-D structures of elasticity and heat transfer subjected to mechanical and thermal loading . It intelligently identifies the critical regions/points in the problem domain and utilize the new concepts of substructuring and wave propagation to choose the proper mesh size for them. INTEMMESH generates well-shaped triangular elements by applying trangulartion and Laplacian smoothing procedures. The adaptive analysis involves the intial finite elements analyze and an efficient ${\alpha}$-posteriori error analysis involves the initial finite element anal sis and an efficient ${\alpha}$-posteriori error analysis and estimation . Once a problem is defined , the system automatically builds a finite element model and analyzes the problem though automatic iterative process until the error reaches a desired level. It has been shown that the proposed approach which initiates the process with an ${\alpha}$-priori, and near optimum mesh of the object , converges to the desired accuracy in less time and at less cost. Such an advanced design/analysis environment will provide the capability for rapid product development and reducing the design cycle time and cost.

• Korean Journal of Computational Design and Engineering
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• v.19 no.2
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• pp.191-201
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• 2014

A 3D CAD system that can be used on a smart device is proposed. Smart devices are now a part of everyday life and also are widely being used in various industry domains. The proposed 3D CAD system would allow modeling in a rapid and intuitive manner on a smart device when an engineer makes a 3D model of a product while moving in an engineering site. There are several obstacles to develop a 3D CAD system on a smart device such as low computing power and the small screen of smart devices, imprecise touch inputs, and transfer problems of created 3D models between PCs and smart devices. The author discusses the system design of a 3D CAD system on a smart device. The selection of the modeling operations, the assignment of touch gestures to these operations, and the construction of a geometric kernel for creating both meshes and a procedural CAD model are introduced. The proposed CAD system has been implemented for validation by user tests and to demonstrate case studies using test examples. Using the proposed system, it is possible to produce a 3D editable model swiftly and simply in a smart device environment to reduce design time of engineers.

• The Journal of Society for e-Business Studies
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• v.2 no.1
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• pp.21-37
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• 1997

As the complexity in design and manufacturing activities of distributed virtual enterprises rapidly increases, the issue of process management becomes more critical to shorten the time-to-market, reduce the manufacturing cost and improve the product quality. This paper proposes a unified framework to manage design and manufacturing processes in a distributed environment. We present a methodology which utilizes process flow graphs to depict the hierarchical structure of workflows and process grammars to represent various design processes and design tools. To implement the proposed concept, we develop a process management system which mainly consists of a cockpit and manager programs, and we finally address a preliminary implementation procedure based on the Object Modeling Technique. Since the proposed framework can be a formal approach to the process management by providing formalism, parallelism, reusability, and flexibility, it can be effectively applied to further application domains of distributed virtual enterprises.