• BMB Reports
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• v.42 no.7
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• pp.418-420
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• 2009

We describe a method for producing polyclonal antibodies against peptide antigen cytochrome P450 1A2 and 3A4 using a tandem repeat of the epitope region and incorporation of proline residue between the repeated sequences. An ELISA assay revealed more efficient generation of polyclonal antibodies to tandem repeat peptide antigens than mono-epitope peptides. The incorporation of proline residues further stimulated antibody production.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.332.1-332.1
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• 2002

Streptococcus vestibularis is a urease-producing oral bacterium. frequently isolated from vestibular mucosa of human oral cavity. Ureolysis by S. vestibularis and other ureolytic oral bacteria is believed to be crucially involved in oral microbial ecology and oral health. Genomic library of the S. vestibularis ATCC49124 was constructed in an E. coli plasmid vector and the urease-positive transformants harboring the urease gene cluster were isolated on Christensen-urea agar plates. The minimal DNA region required for the urease activity was located on a 5.6 kb DNA fragment. (omitted)

• Microbiology and Biotechnology Letters
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• v.28 no.6
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• pp.322-328
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• 2000

To screen agent for the treat-ment of Alzhimers Disease several strains of bacteria producing acetylcholinesterase inhibitor ware isolated from soil. Strain 960903 showed strong acetylcholinesteras inhibitory activity and low butyrylcholinesterse inhibitory activity. The strain 960903 was identified as Pseudomonas sp. Acetylcholinesterase inhibitor ws highly achieved in fermentation medium containing soluble starch 3.0%, glycerol 1.0%, pharmamedia 0.5%, KCI 0.3%, $CaCO_3$ 0.2%, MgS $O_4$..$7H_2$O 0.05%, $KH_2$$PO_4$ 0.05%(pH6.5) at $30^{\circ}C$ for 4 days. Acetylcholinesterase inhibitor was purified by Diaion WA-30($OH^{-}$) column charomatography and cellulose column chromatography. Acetylcholinesterase inhibi-tor showd the maximum wavelength at 205 nm and was soluble in water, acetic acid, ethanol, methanol and dime-thyl sulfoxide. The concentration of 50% inhibition($IC_{50}$) of inhibitor against acetylcholinesterase was 25$\mu\textrm{g}$/ml. The inhibitor was inactivated on heating ar $100^{\circ}C$ fro 15 min and more stable in acidic region than alkaline region.n.

• Journal of the Korean Society of Safety
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• v.17 no.2
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• pp.32-38
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• 2002

In this paper, a new approach for the SynRM(Synchronous Reluctance Motor) control which ensures producing MTPA(Maximum Torque per Ampere) over the entire field weakening region is presented. In addition, this paper presents a speed sensorless control scheme of SynRM using flux observer. Also, by adjusting the base speed for the field weakening operation according to the flux level, the current and voltage limit, the smooth and precise transition into the field weakening operation can be achieved. The validity of the proposed scheme is verified through simulation.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2013.06a
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• pp.315-318
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• 2013

A novel foveation filter method is proposed in DCT domain. For countering the blocking and ringing artifacts, we devise the tools measuring the signal gradient, the block signal variations in the DCT domain. Each measurement is properly applied to each enhancement region and smoothing region. The proposed method optimally adapts the enhancement factors to the characteristics of the underlying signals and so maximizes the enhancement performances with significantly suppressing the artifacts. The subjective and objective evaluations verify that the proposed method sustains producing the improved video qualities for various sequences without tuning any parameters to individual sequences.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2003.06a
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• pp.1605-1608
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• 2003

A unstructured tetrahedral mesh generation algorithm has been presented. To make better meshes in interior region using an advancing front technique, a connecting operator has been developed in addition to the existing operators. Before applying digging operators that generate new nodes inside of a meshing region, a connecting operator is employed that uses existing nodes which satisfy certain conditions for producing well-conditioned elements if possible. By introducing this new operator, tetrahedral meshing process becomes more robust and produces better quality of meshes.

• Molecules and Cells
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• v.23 no.2
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• pp.215-219
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• 2007

In mammals light input resets the central clock of the suprachiasmatic nucleus by inducing secretion of pituitary adenylate cyclase-activating polypeptide (PACAP) from retinal ganglion cells (RGCs). We previously showed that thyroid transcription factor 1 (TTF-1), a homeodomain-containing transcription factor, specifically regulates PACAP gene expression in the rat hypothalamus. In the present study we examined the expression of TTF-1 in PACAP-synthesizing retinal cells. Fluorescence in situ hybridization (FISH) showed that it is abundantly expressed in RGCs of the superior region of the retina, but in only a small subset of RGCs in the inferior region. Double FISH experiments revealed that TTF-1 is exclusively expressed in PACAP-producing RGCs. These results suggest that TTF-1 plays a regulatory role in PACAP-expressing retinal ganglion cells.

• Biomedical Science Letters
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• v.25 no.4
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• pp.372-380
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• 2019

Mice with specific modified genes are useful means of studying development and disease. The CRISPR/Cas9 system is a very powerful and effective tool for generating genetically modified mice in a simple and fast manner. To generate human IgG light kappa constant knock-in mice, we tested by microinjection of a mixture of Cas9 protein, single-guide RNA and target homologous recombinant donor DNA into zygotes. We found that the injection of 10 ng/μL of Cas9 protein and crRNA/tracrRNA, rather than single guide RNA, induced the production of knock-in mice more effectively. Thus, our study provides valuable information that will help to improve the production of knock-in mice and contribute the successful generation of humanized Ab-producing mice in Korea.

• Journal of the Korean Society for Precision Engineering
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• v.21 no.3
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• pp.91-99
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• 2004

An unstructured tetrahedral mesh generation algorithm has been presented. In order to construct better meshes in interior region by using an advancing front technique, a connecting operator and a local finishing operator II have been developed in addition to the existing operators. Before applying digging operators that generate new nodes inside of a meshing region, a connecting operator is employed that uses existing nodes which satisfy certain conditions for producing well-conditioned elements. The local finishing operator II is introduced to terminate the meshing process more flexibly on remaining subregions. With these new operators, tetrahedral meshing process becomes more robust and good quality of meshes are constructed.

• Proceedings of the Korea Multimedia Society Conference
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• 2003.05b
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• pp.431-434
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• 2003

In this paper, we propose an efficient method not only f3r producing accurate region segmentation, solving the over-segmentation problem of watershed algorithm but also f3r reducing post-processing time by reducing computation loads. Through this proposed method, region segmentation of neighboring objects and discrimination of similar intensities were effectively obtained. Input image of watershed algorithm has used the derivative-based detectors such as Sobel and Canny. But proposed method uses the pixels-similarity-based detector, that is, SUSAN. By adopting this proposed method, we can reduce the noise problem and solve the problem of over-segmentation and not lose the edge information of objects. We also propose Zero-Crossing SUSAN. With Zero-Crossing SUSAN, the edge localization, times and computation loads can be improved over those obtained from existing SUSAN