• Title/Summary/Keyword: producing condition

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The Physical Property of the Structural Color Yarn and Fabric for Emotional Garment Using Biomimetic Technology (생체모방기술을 응용한 감성의류용 구조발색사와 직물의 물성)

  • Kim, Hyun-Ah;Kim, Seung-Jin
    • Science of Emotion and Sensibility
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    • v.15 no.1
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    • pp.141-148
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    • 2012
  • This study investigated the structural coloration and fabric hand of the caustic reduced fabrics for emotional garment using structural color yarns, which was spun by 37 alternating nylon and polyester layers capable of producing basic colors using biomimetic technology. The colorations of the three kinds of structural color yarns were confirmed using multi angle spectro-photometer, and their triangular cross sections composed with 37 alternating nylon and polyester layers were measured using SEM and were discussed with layer length in relation with coloration and spinning conditions were also set up. The apparent color difference and reflectance of the three kinds of fabrics with different density and weave pattern were analysed as ranging from 400nm to 700nm. The optimum fabric structural design which is made by warp and weft densities(194ends/in ${\times}$ 105picks/in) and caustic reduction condition by $100^{\circ}C$ temperature and 60minutes with NaOH, 20g/l solution were decided through analysis of the mechanical properties and fabric hands of these three kinds of fabrics treated with 3 kinds of the caustic reduction conditions. And it was shown that the rate of caustic reduction was increased from 13% to 23% with increasing temperature and time of caustic reduction. The extensibility, bending rigidity and shear modulus of caustic reduction treated fabrics were decreased by treatment of caustic reduction, on the other hand fabric compressibility was increased. And it was shown that the hand value of specimen number one which was treated with temperature $100^{\circ}C$ and time 60minute was the best and the hand of this fabric was better than that of Morpho $fabric^{(R)}$ made by Teijin co. Japan.

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Fabrication of Electrospun Juniperus Chinensis Extracts loaded PVA Nanofibers (향나무 추출물을 함유하는 PVA 나노섬유 제조)

  • Kim, Jeong-Hwa;Lee, Jung-Soon
    • Science of Emotion and Sensibility
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    • v.19 no.2
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    • pp.35-42
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    • 2016
  • Electrospinning is a simple and effective process for producing nanofiber with diameter range from nanometers to micrometers which have high specific surface area. Hence, medicated nanofibers can be readily fabricated using a solution containing a mixture of a plant-extracts and a polymer. It has proved that Juniperus Chinensis can be effectively used for the prevention of UV and SLS-induced advers skin reaction such as radical production, inflammation and skin cell damage. It also found that Juniperus Chinensis has efficient ingredient of antifungal activity and house dust mite repellent effect. The fabrication of PVA nanofibers containing Juniperus Chinensis extracts by electrospinning has been studied. PVA/Juniperus Chinensis extracts composite nanofibers were produced at different Juniperus Chinensis concentrations (0.25, 0.5, 1.5 wt. %). The parameters of electrospinning including polymer contents, voltage and tip-to-collector distance (TCD) were optimized for fabrication process. The study show that 12 wt. % PVA, 10kV applied voltage and TCD 10~20 cm are the best condition to obtain uniform PVA/Juniperus Chinensis extracts composite nanofibers. Morphologies of the electrospun composite nanofiber were observed by using a field emission scanning electron microscope. It has been found that the average diameters of fibers increased by the adding of Juniperus Chinensis extracts. As the results, PVA/Juniperus Chinensis extracts composite nanofibers having a diameter in the range from 310~360 nm were successfully prepared via an electrospinning.

Characteristics and Action Pattern of ${\alpha}-galactosidase$ from Scopulariopsis brevicaulis in Korean Traditional Meju (한국 재래 간장에서 분리한 Scopulariopsis brevicaulis가 생성하는 ${\alpha}-galactosidase$의 특성 및 작용양상)

  • Choi, Kwang-Soo;Lee, Seon-Ho;Hong, Seung-Pyo;Lee, Hee-Duck;Bae, Du-Kyung;Choi, Cheong
    • Applied Biological Chemistry
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    • v.41 no.7
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    • pp.489-495
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    • 1998
  • The optimum culture condition of Scopulariopsis brevicaulis for the production of ${\alpha}$-galactosidase was as follows: Tryptone 1.5%, $NH_4NO_3$ 0.2%, Raffinose 2.5%, $KH_2PO_4$ 0.5%, yeast extract 0.5%, pH 7.0, $27^{\circ}C$. The optimum pH and temperature for the enzyme activity of ${\alpha}$-galactosidase producing Scopulariopsis brevicaulis were pH 7.0 and $27^{\circ}C$, respectively. The enzyme was relatively stable at $pH\;6.0{\sim}8.0$ and at temperature below $40^{\circ}C$. The activity of the enzyme was inhibited by $Ag^{2+},\;Hg^{2+},\;Cu^{2+}$, p-chloromercuribenzoic acid and Iodine. These results would indicate the presence of -SH groups in the catalytic site of the enzyme. Km value was 1.9 mM for $p-nitrophenyl-{\alpha}-D-galactopyranoside$ and Vmax value was $9.66{\times}10^2\;{\mu}M/min$. Sugar constituents of culture broth were identified by HPLC that the enzyme liberated sucrose, glucose and fructose from raffinose and raffinose was significantly decreased.

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Freeze Treatment of Sludge for the Biogas Production from Brown Macroalgae (슬러지 냉동처리에 의한 갈조류로부터 바이오가스 생산)

  • Kim, Ji-Youn;Jeong, Haeng Soon;Woo, Dae-Sik;Kim, Sang-Min;Kim, In Soo;Lee, Jae-Hwa
    • Applied Chemistry for Engineering
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    • v.23 no.6
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    • pp.594-598
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    • 2012
  • In the present study, biogas was produced from the anaerobic digestion of marine macroalgae (Laminaria japonica) biomass. The optimal anaerobic condition for producing the sludge was the freeze treatment at $-70^{\circ}C$ for 20 min. Total amounts of hydrogen and methane gas produced were 667.28 mL/L and 3420.24 mL/L, respectively, which were 2.7 and 3.4 times greater than that in the control group. Freeze treatment of sludge produced the maximum biogas under an initial optimum pH of 7.0 and the maximum biomass at an initial optimum pH of 8.0. We confirmed that biogas production was greatly reduced under acidic conditions compared to that under alkaline conditions. Sludge was freeze treated, and the biomass and sludge production was optimal the total amounts of hydrogen and methane gas produced were 643.73 mL/L and 4291.6 mL/L, respectively, which were 2.6 and 4.3 times greater than in the control group. Also the results showed that under optimal conditions in a 5-L bioreactor, a maximum of 1605.03 mL/L of hydrogen and 4593.71 mL/L of methane gas could be produced by the substrate contained in the marine macroalgae biomass.

Purification and characterization of the chitinase from Bacillus subtilis JK-56 (Bacillus subtilis JK-56이 생산하는 chitinase isozyme의 정제와 특성 규명)

  • 전홍기;김낙원;정영기
    • Journal of Life Science
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    • v.12 no.1
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    • pp.77-86
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    • 2002
  • Chitin, a $\beta$-1,4 polymer of N-acetyl-D-glucosamine, is one of the most abundant organic compounds in nature. Chitinase (EC 3.2.1.14) is an enzyme that degrades chitin to chito-oligosaccharides, diacetyl rhitobiose and N-acetyl-D-glucosamine. An extracellular chitinase-producing bacterial strain was isolated from soil and named to as Bacillus subtilis JK-56. Optimum culture condition of B. subtilis JK-56 for the production of chitinase was 1% chitin, 0.5% polypepton, 0.1% KCl, 0.05% MnS $O_4$.4$H_2O$, 37$^{\circ}C$, initial pH 7.0 and 40 hour culture time. When B. subtilis JK-56 was grown in the optimum medium, one major active band and two minor active bands were detected by native-PAGE and active staining of the gel. Among them, the major band was purified from the culture supernatant by 70% ammonium sulfate precipitation and native-PAGE with BIO-RAD Model 491 Prep-Cell and named as Chi-56A. Its molecular weight was estimated to be 53kDa monomer and the isoelectric point (pI) was pH 4.3. The pH and temperature for the optimum activity of Chi-56A were pH 6.0 and $65^{\circ}C$, respectively. Chi-56A was stable up to $65^{\circ}C$ and in alkaline region. Its $K_{m}$ value for colloidal chitin was 17.33g/L. HPLC analysis of the reaction products confirmed that Chi-56A was an exo type chitinase.e.

Changes of Sensory Properties of Alaska Pollack Sikhae during Fermentation (전통 명태식해의 숙성중 관능적 특성 변화)

  • Kim So-Jung;Jeong Eun-Jeong;Kim Hun;Cho Woo-Jin;Kim Kwang-Ho;Lim Chi-Won;Cha Yong-Jun
    • The Korean Journal of Food And Nutrition
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    • v.17 no.4
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    • pp.405-411
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    • 2004
  • The sensory properties in Alaska pollack sikhae were compared in 3 different temperature conditions, 5$^{\circ}C$, 20$^{\circ}C$ and alternating temperature(stored at 5$^{\circ}C$ after 10 days of fermentation at 20$^{\circ}C$), respectively, during fermentation. The change of instrumental texture including hardness, cohesiveness, adhesiveness and chewiness increased and/or decreased without significant difference in the sikhae fermented at 5$^{\circ}C$ during fermentation. Three profiles, hardness, chewiness and cohesiveness in the sikhae fermented at 20$^{\circ}C$ increased up to 12 day and then decreased, whereas those in alternating temperature decreased significantly. From the acceptance test during fermentation, sikhae products fermented for 14 days at 5$^{\circ}C$, 9 days at 20$^{\circ}C$ and 13 days at alternating temperature were superior in sensory properties. The score more over 5 point in overall acceptance was maintained until 14 days in 5$^{\circ}C$, 9 days in 20$^{\circ}C$ and 13 days in alternating temperature, respectively, and particularly, alternating temperature condition was superior to the different temperature conditions. The sensory texture and overall acceptance had the high positive correlation with chewiness and taste, respectively. From the result of quantitative descriptive analysis, the intensities of acidic odor and taste in alternating temperature maintained and/or increased during 27 days of fermentation, whereas those in sikhae fermented at 5$^{\circ}C$ decreased. These results demonstrated that cold chain system such as alternating temperature was needed for shelf-life extension and producing of marketable Allaska pollack sikhae.

Production of Cyclodextrin Glucanotransferase from Aspergillus sp. CC-2-1 and its Characterization (Aspergillus sp. CC-2-1에 의해 생산되는 Cyclodextrin Glucanotransferase의 생산 및 특성)

  • Cho, Young-Je;Kim, Myoung-Uk
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.1158-1167
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    • 2000
  • To produce ${\beta}-cyclodextrin({\beta}-CD)$, a cyclodextrin glucanotransferase(CGTase) producing Aspergillus sp. CC-2-1 was isolated from soil. The enzyme was purified and its enzymological characteristics were investigated. It was found that production of CGTase reached to the maximum when the wheat bran medium containing 0.1% albumin, 2% $(NH_4)_2S_2O_8$, 2% soluble starch and 0.2% $KH_2PO_4$ was cultured for 5 days at $37^{\circ}C$. The purity of CGTase was increased by 13.14 folds after DEAE-cellulose ion exchange chromatography and Sephadex G-100, G-150 gel filtration and the specific activity was 172.14 unit/mg. Purified enzyme was confirmed as a single band by the polyacrylamide gel electrophoresis. The molecular weight of CGTase was estimated to be 27,800 by Sephadex G-100 gel filtration and SDS-polyacrylamide gel electrophoresis. The optimum pH and temperature for the CGTase activity were 9.0 and $80^{\circ}C$, respectively. The enzyme was stable in pH $8.0{\sim}11.0$ at $60{\sim}80^{\circ}C$. The activity of purified enzyme was activated by $K^+,\;Cu^{2+}$ and $Zn^{2+}$. The activity of the CGTase was inhibited by the treatment with 2,4-dinitrophenol and iodine. The result suggests that the purified enzyme has phenolic hydroxyl group of tyrosine, histidine imidazole group and terminal amino group at active site. The reaction of this enzyme followed typical Michaelis-Menten kinetics with the $K_m$ value of 18.182 g/L with the $V_{max}$ of 188.68 ${\mu}mole/min$. The activation energy for the CGTase was calculated by Arrhenius equation was 1.548 kcal/mol.

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Effect of Long Day Period Before Short Day treatment on the Dry Matter Production and Flower Quality of Greenhouse-Grown Cut Chrysanthemum cv, Reagan Improved (단일처리전 장일처리 기간이 온실재배 스프레이 절화국의 건물생산과 절화품질에 미치는 영향)

  • 백철기;이정현;안규빈;한태호;정순주
    • Journal of Bio-Environment Control
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    • v.12 no.1
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    • pp.1-6
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    • 2003
  • This study was conducted under the condition of greenhouse to investigate the relationships between the period of long day (LD) treatment before short day treatment, the dry matter production and flower quality of cut chrysanthemum (Indicum group) cv. Reagan Improved at Wagenigen University in the Netherlands. Rooted cuttings of chrysanthemum (Indicum group) cv. Reagan Improved were transplanted on 6th, 13th, loth of September and all of them were treated with short-days (SD) on 21th September. The periods from planting until final harvest were 70, 77, 84 days after being planted for 1, 2, and 3 week-LD period, respectively. The time of flower initiation was similar in all treatments. The number of flowers per plant was greater in the plot of 3 week-LD period than that in the plot of 1 or 2 week-LD period. The fresh weight and dry weight of flowers also increased more in the plot of 3 week-LD period than that in the 1 week-LD period by 4g and 0.4g, respectively. The final dry matter production ($g{\cdot}m$^{-2}$ ) was greater in the treatment of 3 week-LD period than 1 or 2week-LD period. In this study, LD period before short day treatment did not affect the time of flower initiation and flower quality. As a result, 3 week-LD period before short day treatment was strongly recommanded for producing high quality cut flowers of greenhouse-grown chrysanthemum.

Ameliorating Effects of Nokyongdaebo-tang on Experimental Subacute Hemorrhagic Anemia in Rats (녹용대보탕 열수 추출물의 실험적으로 유발된 랫트 아급성 출혈성 빈혈에 대한 효과)

  • Kim, Jung-Ah;Kim, Dong-Chul
    • The Journal of Korean Obstetrics and Gynecology
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    • v.30 no.3
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    • pp.1-19
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    • 2017
  • Objectives: The object of this study is to observe the possible ameliorating effects of Nokyongdaebo-tang (NYDBT) on the experimental subacute hemorrhagic anemia (SHA) in rats. Methods: In the present study, SHA in rats was induced by exsanguinations from orbital plexus, and ameliorating effects of NYDBT was observed based on the changes of body and hematopoietic organ (spleen, liver and femur) weights, red blood cell (RBC) related hematological values, smear cytology, histopathological changes and immunohistochemistrical analysis of hematopoietic stem cells in the femur bone marrow, liver and spleen. In addition, the gastrointestinal motility and the surface mucosa thicknesses of remnant fecal pellets in the colon lumen, mucosa thicknesses and the mucous producing cell numbers in the colonic mucosa were analyzed to observe the digestive disorders, especially on the constipation, the major discomfort problems in iron supplement. Results: SHA related abnormal anemic signs were markedly and dose-dependently inhibited by oral administration of NYDBT 500, 250 and 125 mg/kg in a condition of this experiment. In addition, no meaningful changes on the gastrointestinal motilities and mucous component on the colon and remnant feces were noticed in all three different dosages of NYDBT treated rats as compared with intact vehicle and SHA control rats in this study. Conclusions: It, therefore, is expected that NYDBT will be promising as a novel alternative hematopoietic and therapeutic agent for anemia.

Physical Properties of the Horticultural Substrate According to Mixing Ratio of Peatmoss, Perlite and Vermiculite (원예용 상토 재료 피트모스, 펄라이트, 버미큘라이트의 혼합비율에 따른 물리적 특성 변화)

  • Kim, Hyuck-Soo;Kim, Kye-Hoon
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.3
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    • pp.321-330
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    • 2011
  • The physical properties of horticultural substrate are important for optimal plant growth. The physical properties should be properly maintained during the crop growing season for producing higher yield. This experiment was carried out to evaluate the physical properties of different mixtures from various raw materials as horticultural substrates. The mixtures at the different ratios of peatmoss, perlite and vermiculite subjected to 10:0:0, 8:2:0, 6:4:0, 4:6:0, 2:8:0, 0:8:2, 0:10:0, 0:6:4, 0:4:6, 0:2:8, 8:0:2, 0:0:10, 6:0:4, 4:0:6, 2:0:8, 2:6:2, 2:4:4, 4:2:4, 4:4:2, 6:2:2 and 2:2:6 were prepared and analyzed according to two methods of the European Standardization (EN) and Rural Development Administration (RDA). The optimum range of physical properties of a specific horticultural substrate can be predicted using physical-property-triangle. This triangle can also be used to convert a physical property from the EN method to that from the RDA method. Results showed that the mixture at a ratio of > 60% peatmoss, in most cases, is in the range of optimum physical condition for plant growth. We conclude that the developed physical-property-triangle can be suitable to suggest the optimum ratios of horticultural substrates used in this study.