• Clinical and Experimental Reproductive Medicine
• /
• v.28 no.3
• /
• pp.183-190
• /
• 2001

Objective: Recently, microdissection of tissue sections has been used increasingly for the isolation of morphologically identified homogeneous cell populations, thus overcoming the obstacle of tissue complexity for the analysis cell-specific expression of macromolecules. The aim of the present study was to establish the minimal conditions required for the RNA extraction and amplification from the cells captured by the laser captured microdissection. Methods : Mouse ovaries were fixed and cut into serial sections (7 im thickness). Oocytes were captured by laser captured microdissection (LCM) method by using PixCell $II^{TM}$ system. The frozen sections were fixed in 70% ethanol and stained with hematoxylin and eosin, while the paraffin sections were stained with Multiple stain. Sections were dehydrated in graded alcohols followed by xylene and air-dried for 20 min prior to LCM. All reactions were performed in ribonuclease free solutions to prevent RNA degradation. After LCM, total RNA extraction from the captured oocytes was performed using the guanidinium isothiocyanate (GITC) solution, and subsequently evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR) for glyceraldehyde-3-phosphate-dehydrogenase (GAPDH). Results: With the frozen sections, detection of the GAPDH mRNA expression in the number of captured 25 oocytes were not repeatable, but the expression was always detectable from 50 oocytes. With 25 oocytes, at least 27 PCR cycles were required, whereas with 50 oocytes, 21 cycles were enough to detect GA PDH expression. Amount of the primary cDNA required for RT-PCR was reduced down to at least 0.25 $\grave{i}$ l with 50 oocytes, thus the resting 19.75 il cDNA can be used for the testing other interested gene expression. Tissue-to-slide, tissue-to-tissue forces were very high in the paraffin sections, thus the greater number of cell procurement was required than the frozen sections. Conclusion: We have described a method for analyzing gene expression at the RNA level with the homogeneously microdissected cells from the small amount of tissues with complexity. We found that LCM coupled with RT-PCR could detect housekeeping gene expression in 50 oocytes captured. This technique can be easily applied for the study of gene expression with the small amount of tissues.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.10
• /
• pp.394-400
• /
• 2018

Recently, it is expected to significantly increase the defense budget by acquiring large-scale weapons systems to enhance defensive capabilities. In order to secure funds needed to acquire such weapons systems, it is necessary to improve the efficiency of the defense budget. In addition, interest in economic factors affecting the domestic economy such as export opportunities and employment creation are increasing, as well as budget reduction of the defense acquisition business. In order to respond to these demands, this study conducted cost- benefit analysis and examined economic ripple effects in the implementation of defense procurement projects. However, research on economic analysis methodology considering characteristics of defense acquisition projects is still in its early stage. This study proposes a methodology to analyze the economic feasibility of defense acquisition projects and conducted a case study on the economic feasibility of the ${\bigcirc}{\bigcirc}$ guided missile project which is being prepared against local provocation. The proposed economic analysis method provides the cost-effectiveness analysis that has been performed in many weapon system acquisition projects in connection with the more general cost-benefit analysis method. This study contributes to the establishment of an improved theoretical methodology for weapon system acquisition projects.

• Journal of Life Science
• /
• v.20 no.1
• /
• pp.60-65
• /
• 2010

Since organic sulfur compounds respond to GC/ECD sensitively, they interfere with quantitative separation of pesticides during residual pesticide analysis of Allium species. In this study, it was intended to develop a rapid and simple method for pesticide multi-residues analysis through clean-up and interferences by a solid-phase extraction (SPE). An SPE method employing silver nitrate impregnated Florosil cartridge was developed and evaluated for the elimination of sulfur compounds from the test solution of Allium species during pesticide residues analysis. The silver nitrate impregnated Florosil cartridge was prepared by efflux of 3 ml of 20% silver nitrate solution through Florosil cartridge (1 g packing, 6 ml tube). The extracts equivalent to 2, 4 6, and 10 g of each sample were loaded onto the cartridge and allowed to exude, and then the exudations were analyzed by GC/ECD. More than 95% of sulfur compounds were removed from the loaded extracts equivalent, up to 6 g in onion, 4 g in spring onion and 4 g in shallot, respectively. 40 pesticides were spiked in the Allium species and loaded onto the cartridge to determine the recoveries; from this, the recoveries of 34 pesticides were within 70~120%.

• Korean Journal of Construction Engineering and Management
• /
• v.9 no.5
• /
• pp.168-175
• /
• 2008

Project risk factors are major triggers in cost performance in construction projects. Both owners and contractors are highly concerned in managing their risks in earlier times. As such, it is needed to provide a robust risk evaluation methodology in identifying and assessing the potential risks before project execution. In previous research, risk identification was conducted only in specific phases, i. e., design, procurement, construction. Therefore, the comprehensive approach in risk management was limited and their quantifying method was not well-defined. Since the benefit of planning in earlier times are maximized compared to later times, the risk management should be implemented in earlier planning stage. This study provide a new risk evaluation method by incorporating previous research and extensive literature review. By quantifying each risk factor from an extensive industry survey, the proposed tool can enhance the reliability of each factor weight and also the weights are categorized from a various perspectives, i.e., owners, contractors, lager-scale company, smaller-scale company, building projects. The proposed tool can be also useful in deciding on whether to proceed a particular project and How much the project contingency would be set aside in project execution. The study findings can also expedite the risk management procedure in a more systematic approach.

• Korean Journal of Construction Engineering and Management
• /
• v.4 no.3 s.15
• /
• pp.128-136
• /
• 2003

The contractors which execute the design-build project have difficulties in design management and contract administration in the pre-construction phase because they have managed construction projects focused on only construction phase so far. Moreover, they have to manage the design development stage without any contingency following-up various design revisions from the design reviews of outside experts. Therefore, the design management in the design development stage is a critical success facto. of the design-build project. The objective of this study is to structure the problems in the management skill for design development phase in the domestic design-build projects and to suggest a design development management method to settle the problems. To achieve these objectives, this study 1) analyzes the prior studies about the design-build procurement methods, 2) designs and performs a case study to embody the problems of the domestic design-build projects, and 3) proposes a method of design development management by analyzing the results of case study and interviewing experts.

• Journal of Environmental Health Sciences
• /
• v.49 no.3
• /
• pp.134-148
• /
• 2023

Background: The conducting of health effect surveys (HESs) in environmentally contaminated vulnerable areas (ECVAs) by the central and local governments has been increasing apace with the increase in demand for HESs since the Environmental Health Act was enacted in South Korea in 2008. Objectives: This study aimed to review the HESs of residents in ECVAs conducted in South Korea. Methods: An analysis was performed on 125 reports obtained from the Environment Digital Library, PRISM, and local government websites after selecting from 803 projects obtained as ECVAs from the Korea ON-Line E-Procurement System (1997~2021), National Institute Environment Research (2000~2021), and Korea Environmental Industry and Technology Institute (2009~2021). The reports were classified by background (residents' demand, HES, and more), research design (cross-sectional study, cohort, ecological study, and panel), pollution source (abandoned metal mine (AMM), industrial complex (IC), and more), and assessment method of exposure and health effects. The survey area was converted into administrative district codes for geographical mapping. Results: There were 37, 34, 18, and 10 cases associated with AMM, IC, waste incinerators, and coal-fired power plants, respectively. Most of the studies conducted were cross-sectional studies and ecological studies. The proportion of epidemiological investigations by residents' demand showed an increase from 0.0% to 8.9% for the central government while decreasing from 16.7% to 14.3% for local governments after 2008 compared to before 2008. HESs increased at both the central and local government levels since 2014. For the evaluation method, 365 environmental hazards, 319 health outcomes, and 302 biological markers were investigated, with the most commonly investigated items being metals, cancer, and blood metals. Conclusions: HESs of residents in ECVAs in South Korea have been continuously developed both quantitatively and qualitatively. Future improvements are expected, and systematic review and classification of the HESs is warranted.

• Korean Journal of Construction Engineering and Management
• /
• v.21 no.2
• /
• pp.79-87
• /
• 2020

The Korean domestic construction management at risk (CMAR) market is in the process of completing the pilot project execution under the leadership of the Ministry of Land, Infrastructure and Transport as of December 2019. The government starts practicing CMAR an alternative delivery method widely in order to diversify delivery methods and enhance construction technology. The CMAR market is thus expected to grow. This study was conducted to improve CMAR firms' capability by developing self-assessment tools for them to evaluate current capability more effectively. As a result of defining standard core capability and additional elements categorized by project execution phase and management area, and performing evaluation from the CMAR project participants, it was found that the general project management capability in the pre-design and procurement phase and quality management area was lower compared to the construction phase and other areas. In addition, the capability of cost management area was lower in spite of its high importance. Communication and coordination, process optimization, and target values achievement were at the initial level of capability and continuous improvement was required.

• Land and Housing Review
• /
• v.8 no.1
• /
• pp.23-29
• /
• 2017

The BIM design is expected to expand to the domestic and overseas construction industries, depending on the effect of construction productivity and quality improvement. However, with the obligation of Public Procurement Service to design the BIM design, it includes a design error and the problem of utilization of 3D design by choosing a simple 2D to 3D remodelling method that can not be modelled in 3D modeling or use of the construction and maintenance phases. The results reviewed by BIM design results were largely underutilized and were not even performed with the verification of the error. In order to resolve this, one must develop the check system that secures the quality of BIM design and ensure that the reliability of BIM results are available. In this study, it is designed to develop a program that can automatically verify the design of the BIM design results such as violation of the rules of the BIM design, design flaws, and improve the usability of the BIM design. In particular, this programs were developed not only to identify programmes that were not commercially available, but also to validate drawings in low-light computer environments. The developed program(LH-BIM) store the information of attribute extracted from the Revit file(ArchiCAD, IFC file included) in the integrated DB. This provides the ability to freely lookup the features and properties of drawings delivered exclusively by the LH-BIM Program without using the Revit tools. By doing so, it was possible to resolve the difficulties of using traditional commercial programs and to ensure that they operate only with traditional PC performance. Further, the results of the various BIM software can be readily validated, which can be solved the conversion process error of IFC in the case of SMC. Additionally, the developed program has the ability to automatically check the error and design criteria of the drawings, as well as the ability to calculate the area estimation. These functions allow businesses to apply simple and easy tasks to operate tasks of BIM modelling. The developed system(LH-BIM) carried out a verification test by reviewing the review of the BIM Design model of the Korea Land & Housing Corporation. It is hoped that the verification system will not only be able to achieve the Quality of BIM design, but also contribute to the expansion of BIM and future construction BIM.

• The Journal of Engineering Geology
• /
• v.20 no.2
• /
• pp.191-202
• /
• 2010

In this study, the relationship between the settlements and the horizontal displacements according to embankment was analyzed at the marine sedimentary grounds for preparation of a site, and then the empirical equations of both the settlement and the horizontal displacement considering the embankment load and the thickness were proposed. To do this, the field and laboratory tests were performed at the improvement section where the pre-loading method was applied, and the field monitoring was performed using various sensors. Based on the results of the tests and monitoring, the ground deposits, soil characteristics and engineering properties were analyzed and the settlements and lateral movements were estimated by the Regression analysis. The ground deposits from the ground surface were composed of reclaimed soils, sedimentary soils and based rocks. The thickness of clay in the sedimentary soils layer was ranged from 3.9 m to 44.5 m. The embankment heights to improve the ground during pre-loading were constructed from 4.7 m to 7.8 m in each section. The settlements during embankment were ranged from 0.959 m to 2.217 m and the lateral movements were ranged from 0.048 m to 0.313 m. As the result of regression analysis, the equations of settlements and horizontal displacements according to embankments may be proposed as $s=0.02h^2+0.11h$ and ${\delta}=0.01e^{0.37h}$, respectively. The proposed empirical equations of the settlements and the horizontal displacements according to embankment on the marine sedimentary ground may be applied to the site where has a similar condition of study area.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.5
• /
• pp.257-265
• /
• 2020

Parts obsolescence is a component type, in which raw materials or parts that make up a weapons system, are developed and produced according to demand, but they age due to changes in technology or market conditions. The weapon system equipment currently being used by the military is having difficulty in procurement, maintenance, and operation because of the obsolescence of parts. Parts obsolescence during the life cycle of a weapons system affects not only the combat readiness of the military but also the economic loss of material, parts, and equipment manufacturers and suppliers. On the other hand, advanced technology has reduced the life span of electronic components that make up the weapons system. In addition, because of the nature of the weapons system, the obsolescence of components takes decades from development to disposal, which has a serious adverse effect on equipment readiness and life cycle costs. Therefore, it is necessary to manage systematically the parts obsolescence from development until the disposal of the weapons system equipment. This study examined the system of component control in developed countries to present a proper management plan for local situations and propose development plans for the early settlement of component control.