• Clinical and Experimental Reproductive Medicine
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• v.51 no.1
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• pp.42-47
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• 2024

Objective: This study evaluated the effects of temperature and storage time on the quality and DNA integrity of freeze-dried sperm from individuals with normozoospermia. Methods: Normal sperm samples from 15 men aged 24 to 40 years were studied. Each sample was divided into six groups: fresh, freezing (frozen in liquid nitrogen), freeze-dried then preserved at room temperature for 1 month (FD-1m-RT), freeze-dried then preserved at room temperature for 2 months (FD-2m-RT), freeze-dried then preserved at 4 ℃ for 1 month (FD-1m-4 ℃), and freeze-dried then preserved at 4 ℃ for 2 months (FD-2m-4 ℃). The morphology, progressive motility, vitality, and DNA integrity of the sperm were evaluated in all groups. Results: In all freeze-dried groups, sperm cells were immotile after rehydration. The freeze-dried groups also showed significantly less sperm vitality than the fresh and frozen groups. Significantly more morphological sperm abnormalities were found in the freeze-dried groups, but freeze-drying did not lead to a significantly higher DNA fragmentation index (DFI). The DFI was significantly higher in the FD-2m-RT group than in the other freeze-dried groups. Conclusion: The freeze-drying method preserved the integrity of sperm DNA. The temperature and duration of storage were also identified as factors that influenced the DFI. Accordingly, more research is needed on ways to improve sperm quality in the freeze-drying process.

• Journal of information and communication convergence engineering
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• v.8 no.3
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• pp.339-342
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• 2010

An iris image pattern positioning with preserved edge detector, ring zone and clock zone, frequency distribution and overlay matching is presented in this paper. Edge detector is required to be powerful and detail. That is proposed by overlaying Canny with LOG (CLOG). The two reference patterns are made from allocating each gray level on the clock zone and ring zone respectively. The normalized target image is overlaid with the clock zone reference pattern and the ring zone pattern to extract overlapped number, and make a matched frequency distribution to look through a symptom and position of human organ and tissue. The iterating experiments result in the ring and clock zone positioning evaluation.

• Journal of the Korean Home Economics Association
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• v.18 no.4
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• pp.87-94
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• 1980

This study was carried out to make a comparison between iced and partial freezing of bastard, yellow tail, poragy, pomfret that were generally used for Sashimi and the results that measured k-value, VB-N, TMA-N were measured and the results are as follows : 1. Regardless of the kinds of fish, freshness is better preserved in partial freezing than in iced storage. 2. In Bastard, Yellow tail When iced its freshness estimation index its K-value rose above 20% after 4 days of storage. When stored partially frozen, its K-value reached 20% after 8 days. 3. Porgy when iced, its K-value reach 20% after 6 days of storage. But when stored partially frozen, its K-value could be prolonged until 9 days with same degree. Porge was preserved for the longest time among the four fishes. 4. Pomfret When iced during 4 days and stored partially frozen during 6 days, their K-value reached about 20% Pomfret was preserved for the shortest time among the four fishes. 5. According to the kinds of fish, the results that measured VB-N, TMA-N, total bacteria have some differences, but the method of partial freezing was superior to iced storage.

• Journal of Conservation Science
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• v.35 no.6
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• pp.588-599
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• 2019

Conservation measures for excavated and surveyed buried cultural heritages are decided by the administrator of the Cultural Heritage Administration according to their scientific and historic values. However, management, preservation, and use after relocation and restoration remain unsystematic. An issue regarding preservation plan and use has arisen due to the damage of preserved heritages and protective facilities. Thus, this study aims to suggest a plan for preservation and use by investigating the present conditions of the preserved heritages in the Chungbuk area. Results showed that there are 43 preserved heritages in Chungbuk, most of which remain unmanaged systematically irrespective of the managing body. Remainder and protective facilities have been damaged due to wrong preservation treatments and selection of materials, and a problem also rises in terms of utilization, such as exhibition, education, and experience of preserved heritages. To improve such problems, a medium and long-term plan shall be established for the improvement of legal and institutional instruments, securing of budget, increase in professional manpower, development of standardization and inspection manuals, continuous monitoring, preventive preservation, research on relocation methods and materials, listing, and the creation of specialized museum and complex theme park. If a preservation plan suited to the investigated heritages can be developed, heritages can be preserved and managed more systematically and scientifically, and be used for various purposes like education, exhibition, public relations, etc.

• Journal of Embryo Transfer
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• v.33 no.1
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• pp.23-30
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• 2018

The purpose of this study was to investigate the effects of the concentration of seminal plasma in aerobic and anaerobic conditions on the total motility(TM) and the progressive motility(PM) of spermatozoa in long term preservation of cooled equine semen. We also examine the pregnancy rates after artificial insemination using fresh, cooled or frozen semen, and different durations of cooled-preserved equine semen. In the aerobic state of cooled-preserved semen, As the increase of preserved duration to 24h, 48h, 72h, and 96h, TM tended to decrease in each of different concentrations of formalin-containing experimental group, TM tended to decrease regardless of the concentrations of SP. In different concentrations of SP, TM of without seminal plasma(SP W/O) group tended to be higher than that of SP 20%, SP 33% and SP 50%, especially TM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). PM was higher in the groups of SP W/O and SP 20% than in the groups of SP 33% and SP 50% from 24 h to 72 h in cooled-preservation, especially PM of SP W/O group was significantly higher than other groups at 96 h (p<0.05). In the anaerobic condition of cooled-preserved semen, the results of TM and PM at different concentrations of SP were similar to the results in the aerobic condition although there was a difference in the ratio. The pregnancy rates of fresh-cooled, cooled-preserved and frozen semen were 66.3%, 60.7% and 34.5%, respectively, and the pregnancy rate of frozen semen was the lowest. We also found that it is possible to pregnancy after artificial insemination using 72 h cooled-preserved equine semen. There was similar of the pregnancy rates in the different month from April to August.

• Journal of Chest Surgery
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• v.24 no.7
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• pp.712-718
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• 1991

The pectus excavatum or funnel shaped thorax is a relatively frequent malformation of the chest wall which composed of depressed sternum and costal cartilage. The so-called sternal turnover operation for pectus excavatum has been disappointing due to postoperative complication such as fistula formation with necrosis of bone and muscle. Thus, the need of preserved vascular pedicle to sternum was stressed in this type of operation, keeping the bilateral internal mammary vessels preserved. We have experienced two cases of modified sternal turnover in children and obtained satisfactory postoperative results.

• Journal of the Korean Institute of Intelligent Systems
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• v.3 no.4
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• pp.21-23
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• 1993

Under the maxmin compositional rule of inference which is used in applications while executing fuzzy algorithms, Pappis showed that the property of approximation is preserved. In this paper, we generalize a measure of proximity of fuzzy subsets on any set, without the restriction of finiteness. And it is shown that the same property of approximation if preserved under the supmin compositional rule of inference.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.4
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• pp.526-530
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• 2003

Changes of physicochemical properties of brined cucumber were investigated in the samples mixed cucumber and water at 1 : 1.2 ratio in various salt concentrations from 15 to 30%. As salt concentrations in brine solutions were increased, cucumber was getting constricted and decreased in the size of diameter. Salt concentrations of brined cucumber were reached an equilibrium after 30 days of brine storage. When cucumber was preserved at 30% salt concentration, pH was gradually decreased until 60 days of brine storage. When cucumber was preserved in 15% salt solution, the yellowness on the surface of cucumber peels was the most intense among the groups. Among the groups preserved by more than 20% of salt, no difference was observed in intensity of yellowness after 30 days of brine storage. The absorbances of isopropyl alcohol extracts at 410, 505, 607, and 665 nm were different from those of raw cucumbers. Regardless of salt concentration, the hardness of cucumber was increased as preservation was prolonged except the group preserved in 15% salt solution.

• Journal of Korean Society of Steel Construction
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• v.12 no.3 s.46
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• pp.259-268
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• 2000

In the friction-type joints the external applied load is transmitted by frictional force acting on the contact area of the plates fastened by the high strength bolts. This frictional force is proportional to the product of the bolt clamping force and slip coefficient of the faying surface. But the bolt clamping force is dependent on many factors when the turn-of-nut method is used. The preserved thread length and length-to-diameter ratios are one of the major factors governing the bolt clamping force. This paper presents the correct method of high strength bolt tightening through the experiment on the mechanical properties on sets of high strength bolts in accordance with preserved thread length and length-to-diameter ratios.

• Asian-Australasian Journal of Animal Sciences
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• v.9 no.1
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• pp.91-99
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• 1996

About 7.7 million tons of straw dry matter are being rotten during the monsoon. The objective of this trial was to develop a technique to preserve straw under wet condition. To simulate the moisture content of wet straw, a dry straw was deeped overnight in water. After draining the excess water, the wet straw(668 g moisture $kg^{-1}$) was divided into twenty fractions and preserved with 0, 30, 50 ro 70 g urea $kg^{-1}$ dry matter for either of 30, 60, 90, 120 or 180 days in sealed plastic container. Considering the colour, smell, fungal infestation and pH, the wert straw was preserved excellently up to 180 days when 50 or 70 g of urea per kg DM was used. Urea preservation increased the crude protein contents of straw by 3.6 to 6.4 times (174 to $364g{\cdot}kg^{-1}$) over that of the dry straw ($48g{\cdot}kg^{-1}$). Although the NDF content of straw was not effected by the level of urea or by the length of the preservation period, but the ADF content increased (p > 0.05) by 0.086 to $0.889g{\cdot}kg^{-1}$ straw DM for each g increase in the urea level. At 48 hours, the DM degradability of dry straw was $350g{\cdot}kg^{-1}$, which increased to $633g{\cdot}kg^{-1}$ when preserved with 50 g urea $kg^{-1}$ for 180 days. For the same straw, both the rate(0.0388 vs. 0.0136 fraction $h^{-1}$), the extent(717 vs. $631g{\cdot}kg^{-1}$) of straw degradation and the estimated ME(9.55 vs. $6.51MJ{\cdot}kg^{-1}$ straw DM) were higher in the preserved than the dry straw.